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http://dx.doi.org/10.5395/JKACD.2007.32.5.419

Evaluation of the radiopacity and cytotoxicity of resinous root canal sealers  

Kim, Chang-Kyu (Department of Conservative Dentistry, College of Dentistry, Dankook University)
Ryu, Hyun-Wook (Department of Conservative Dentistry, College of Dentistry, Wonkwang University)
Chang, Hoon-Sang (Department of Conservative Dentistry, College of Dentistry, Wonkwang University)
Lee, Byung-Do (Department of Oral and Maxillofacial Radiology, College of Dentistry, Wonkwang University)
Min, Kyung-San (Department of Conservative Dentistry, College of Dentistry, Wonkwang University)
Hong, Chan-Ui (Department of Conservative Dentistry, College of Dentistry, Dankook University)
Publication Information
Restorative Dentistry and Endodontics / v.32, no.5, 2007 , pp. 419-425 More about this Journal
Abstract
The aim of this study was to evaluate the radiopacity and cytotoxicity of three resin-based (AH 26, EZ fill and AD Seal), a zinc oxide-eugenol-based (ZOB Seal), and a calcium hydroxide-based (Sealapex) root canal sealers. Specimens, 10 mm in diameter and 1 mm in thickness, were radiographed simultaneously with an aluminum step wedge using occlusal films, according to ISO 6876/2001 standards. Radiographs were digitized, and the radiopacity of sealers was compared to the different thicknesses of the aluminum step wedge, using the Scion image software. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the cytotoxicity of each material was determined in immortalized human periodontal ligament (IPDL) cells. The results demonstrated that EZ fill was the most radiopaque sealer, while Sealapex was the least radiopaque (p < 0.05). AH 26, AD Seal and ZOB Seal presented intermediate radiopacity values. All the materials evaluated, except for Sealapex, presented the minimum radiopacity required by ISO standards. The cell viabilities of resin-based root canal sealers were statistically higher than that of other type of root canal sealers through the all experimental time. Further, EZ fill showed statistically lower cell viability in 24 and 48 hours compared to AD Seal and in 72 hours compared to all other resin-based root canal sealers. However, there was no correlation between the radiopacity and cytotoxicity of three resin-based root canals sealers (p > 0.05). These results indicate that resin-based root canal sealer is more biocompatible and has advantage in terms of radiopacity.
Keywords
Radiopacity; Cytotoxicity; Root canal sealer; MTT assay; Aluminum step wedge; Immortalized human periodontal ligament cell;
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1 Imai Y. Komabayashi T. Properties of a new injectable type of root canal filling resin and adhesiveness to dentin. J Endod 29:20-3, 2003   DOI   ScienceOn
2 Eliasson ST, Haasken B. Radiopacity of impression maerials. Oral Surg Oral Med Oral Pathol 47:485-91, 1979   DOI   ScienceOn
3 Camps J, About I. Cytotoxic testing of endodontic sealers: a new method. J Endod 29:583-6, 2003   DOI   ScienceOn
4 Baksi Akdeniz BG, Eyuboglu TF, Sen BH, Erdilek N. The effect of three different sealers on the radiopacity of root fillings in simulated canals. Oral Surg Oral Med Oral Pathol Oral Radial Endod 103:138-41. 2007   DOI   ScienceOn
5 Aoyagi Y, Takahashi H, Iwasaki N, Honda E, Kurabayashi T. Radiopacity of experimental composite resins containing radiopaque materials. Dent Mater J 24:315-20. 2005   DOI   PUBMED   ScienceOn
6 Kuga MC, Moraes IG, Berbert A. Capacidade seladora do cimento Seal apex puro ou acrescido de iodofomio. Rev Odontol USP 2: 149-42. 1988
7 Munaco F. Miller W. Mona E. A study of long-term toxicity of endodontic materials with use of an in vitro model. J Endod 4:151-7, 1978   DOI   ScienceOn
8 Matsumoto K, Inoue K, Matsumoto A. The effect of newly developed root canal sealers on rat pulp cells in primary culture. J Endod 15:60-7, 1989   DOI   ScienceOn
9 Lindqvist L, Otteskog P. Eugenol: liberation from dental materials and effect on human diploid fibroblast cells. Scand J Dent Res 89:552-6, 1981
10 Valle FG, Taintor JF, Marsh CL. The effect of varying Iiquid-to-powder ratio to zinc oxide and eugenol of rat pulpal respiration. J Endod 6:400-4, 1980   DOI   ScienceOn
11 Laghios CD, Benson BW, Gutmann JL, Cutler CW. Comparative radiopacity of tetracalcium phosphate and other root-end filling materials. Int Endod J 33:311-5, 2000   DOI   ScienceOn
12 Spangberg LSW, Barbosa SV, Lavigne GD. AH 26 release formaldehyde. J Endod 19:596-8. 1994   DOI   ScienceOn
13 Higginbotham TL. A comparative study of physical properties of five commonly used root canal sealers. Oral Surg Oral Med Oral Pathol 24:89-101, 1967   DOI
14 Cox ST, Hembree JH. McKnight JP. The bactericidal potential of various endodontic materials for primary teeth. Oral Surg Oral Med Oral Pathol 45:947-54, 1978   DOI   ScienceOn
15 Azar NG, Heidari M, Bahrami ZS, Shokri F. In vitro cytotoxicity of a new epoxy resin root canal sealer. J Endod 26:462-5, 2000   DOI   ScienceOn
16 Kettering JD, Torabinejad M. Cytotoxicity of root canal sealers: a study using HeLa cells and fibroblasts. Int Endod J 17:60-6, 1984   DOI
17 Spangberg L, Langeland K. Biologic effect of dental materials. 1. Toxicity of root canal filling materials on HeLa cells in vitro. Oral Surg 35 :402-14. 1973   DOI   ScienceOn
18 de Oliveira Mendes ST, Ribeiro Sobrinho AP, de Carvalho AT, de Souza Cortes MI, Vieira LQ. Invitro evaluation of the cytotoxicity of two root canal sealers on macrophage activity. J Endod 29:95-9, 2003   DOI   ScienceOn
19 Limkangwalmongkol S, Abbott PV, Sandler AB. Apical dye penetration with four root canal sealers and guttapercha using longitudinal sectioning. J Endod 18: 535, 1992
20 Pi SH, Lee SK, Hwang YS, Choi MG, Lee SK, Kim EC. Differential expression of periodontal ligament-specific markers and osteogenic differentiation in human papilloma virus 16-immortalized human gingival fibroblasts and periodontal ligament cells. J Perio Res 42: 104-13, 2007   DOI   ScienceOn
21 Wilson AD, Batchelor RF. Zinc oxide-eugenol cements: IT. Study of erosion and disintegration. J Dent Res 49:593-8, 1970   DOI   PUBMED   ScienceOn
22 Mittal M, Chandra S. Comparative tissue toxicity evaluation of four endodontic sealers. J Endod 21 :622-4. 1995   DOI   ScienceOn
23 Maseki T, Nakata K, Kohsaka T, Kobayashi F, Hirano S, Nakamura H. Lack of correlation between the amount of eugenol released from zinc oxide-eugenol sealer and cytotoxicity of the sealer. J Endod 17: 76-9, 1991   DOI   ScienceOn
24 Yesilsoy C, Feigal RJ. Effect of endodontic materials on cell viability across standard pore size filters. J Endod 11:401-7, 1985   DOI   ScienceOn
25 Beyer-Olsen EM, Orstavik D. Radiopacity of root canal sealers. Oral Surg Oral Med Oral Pathol 51: 320-8, 1981   DOI   ScienceOn
26 Koulaouzidou EA, Papazisis KT, Economides NA, Beltes P, Kortsaris AH. Antiproliferative effect of mineraI trioxide aggregate. zinc oxide-eugenol cement, and glass-ionomer cement against three fibroblastic cell lines. J Endod 31:44-6, 2005   DOI   ScienceOn
27 Tai KW, Huang FM, Chang YC. Cytotoxic evaluation of root canal filling materials on primary human oral fibroblast cultures and a permanent hamster cell line. J Endod 27:571-3, 2001   DOI   ScienceOn
28 Watts DC, McCabe JF. Aluminum radiopacity standards for dentistry: an international survey. J Dent 27:73-8, 1999   DOI   ScienceOn
29 Katz A, Kaffe I, Littner M, Tagger M, Tamse A. Densitometric measurement of radiopacity of guttapercha cones and root dentin. J Endod 16:211-3, 1990   DOI   ScienceOn
30 McComb D. Smith DC. Comparison of physical properties of polycarboxylate-based and conventional root canal sealers. J Endod 2:228-35, 1976   DOI   ScienceOn
31 Hume VR. Effect of eugenol of respiration and division in human pulp, mouse fibroblasts, and liver cells in vitro. J Dent Res 63: 1262-5. 1984   DOI   PUBMED   ScienceOn
32 Schafer E, Zandbiglari T. Solubility of root-canal sealers in water and artificial saliva. Int Endod J 36; 6609, 2003
33 Geusten W, Leyhausen. Biological aspects of root canal filling materials-histocompatibility, cytotoxicity and mutagenicity. Clin Oral In vest 1: 5-11, 1997   DOI   ScienceOn