• Journal of dental hygiene science
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• v.13 no.3
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• pp.321-329
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• 2013

Although it has been reported that lysyl oxidase (LOX) is involved in odontoblastic differentiation, the role of LOX on odontoblastic differentiation by hydrogen peroxide ($H_2O_2$) have not been clarified. In the present study, we investigated whether $H_2O_2$, reactive oxygen species (ROS), is modulated the messenger RNA (mRNA) expression and activity of LOX during odontoblastic differentiation of human dental pulp (HDP) cells. The mRNA expression was quantified by reverse transcriptase polymerase chain reaction (RT-PCR) analysis, and LOX enzyme activity was measured by high sensitive fluorescent assay. Expression of the odontoblastic differentiation marker genes were assessed in the presence and absence of specific small interfering RNAs (siRNAs) of the LOX and LOXL. The $H_2O_2$-induced mRNA expression of LOX family was significant reduction of LOX, LOXL, and LOXL3 mRNA levels in HDP cells. LOX enzyme activity was increased at $H_2O_2$ 0.3 mM for 24 hours. The mRNA expression of alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCN) was inhibited by LOX- and LOXL-specific siRNAs whereas the mRNA expression of dentin matrix protein1 (DMP1), and dentin sialophosphoprotein (DSPP) was inhibited by LOX-specific siRNA. In LOX enzyme activity, siRNA-induced knockdown of both LOX and LOXL inhibited the total amine oxidase activity in HDP cells, as in the case of mRNA expression. In conclusion, the essential role of $H_2O_2$ on odontoblastic differentiation suggests that its regulation by LOX may have pharmacologic importance in HDP cells.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.10
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• pp.1460-1466
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• 2012

The purpose of this study was to evaluate plasticizer pollution levels in mudflat solar salt, salt water and sea water of the nationwide saltpan. Also, it was analyzed the relationship between DEHP in solar salt and PVC mat following private period. DEP (ranging from 0.00 to 1.55 ppb), DIBP (0.00~2.38), DBP (0.00~4.90), DEHA (0.00~2.32), and BBP (0.00~1.45) in solar salt were shown a extremely low concentrations, and DEHP was present a concentrations ranging from 0.00 to 268.5 ppb in solar salt. Further, DMP, DPrP, DNPP, DNHP, and DCHP were not detected in all solar salt. Phthalate in sea water and salt water was present a infinitesimal amount levels. DEHP levels in sea water and salt water were not shown a high risk levels ranging from 0.00 to 3.4 ppb, and from 0.00 to 21.4 ppb, respectively. As expected in PVC mat of nationwide saltpan, the correlation between DEHP in solar salt and PVC mat private period showed a low positive correlation coefficient ($r^2$=0.0362).

• Proceedings of the Korean Society for Information Management Conference
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• 2017.08a
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• pp.100-100
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• 2017

100년의 역사를 지닌 한국지질자원연구원(KIGAM)은 국내 유일의 지질자원 전문연구기관으로서 그간 생산한 조사 연구데이터는 우리나라 과학기술의 귀중한 역사적 학술적 가치가 큰 유산으로써 보존 가치가 매우 크다고 할 수 있다. 하지만 현재 KIGAM의 상황은 최종성과물 위주로 자료관리가 이루어지고 있으며, 조사 연구 과정에서 생산된 암석 토양 지하수샘플이나 조사 탐사장비를 통해 얻어지는 자료는 연구자 또는 연구실 팀에서 개별적으로 관리하고 있다. 이러한 자료관리체계는 자료의 공동 활용이 어렵고, 자료를 보유하고 있는 연구자의 퇴직이나, 조직개편으로 인한 팀 실의 분리 과정에서 자료의 손실과 훼손 가능성이 높고, 누가 어디에 어떤 자료를 무슨 형태로 보관하고 있는지 찾기 어려워 자료의 재활용도가 떨어질 뿐만 아니라, 이로 인한 중복 조사 연구 가능성도 배제할 수 없다. KIGAM은 지질자원분야 국가데이터센터 구축을 목표로 연구과정에서 생산되는 연구데이터의 체계적인 관리와 공유, 활용체계 구축을 위해 2015년도에 기획사업을 통해 중장기 로드맵을 포함한 추진전략을 수립하였으며, 한국과학기술정보연구원(KISTI)의 DataNest를 기술이전받아 지질자원 연구데이터 리포지토리 시스템(GDR: Geoscience Data Repository)를 개발하였다. GDR 시스템 개발을 위해 연구데이터 분류코드를 작성하였으며, 2016년부터 데이터관리계획(DMP: Data Management Plan)을 주요사업 연구계획서 양식에 포함시켜 제출하도록 하였다. 과거 KIGAM은 연구데이터를 수집, 관리하기 위해 몇 차례에 걸쳐 시도를 했지만 실패한 경험을 가지고 있다. 실패 요인에는 (1) 관련 정책, 제도, 조직, 인력, 예산 등 데이터 관리 인프라 부재, (2) 연구사업에서 생산된 데이터는 개인소유라는 인식 및 공유 의식 부족, (3) 데이터 관리 활동은 귀찮은 것이고, 시간 낭비라는 인식, (4) 데이터 관리 공개 공유 활동에 대한 보상체계 부재 등을 꼽을 수 있다. 즉, 제도를 포함한 인프라 부족과 경영진과 구성원의 인식부족이 제일 큰 원인으로 판단된다. 성공적인 연구데이터 관리를 위해서는 지속적이고 꾸준한 투자가 이루어져야 하지만 경영진의 의지에 따라 사업이 중단되기도 한다. 이러한 과거의 실패 요인에 대한 해결 없이 지난 1년 6개월 정도의 GDR 운영은 지지부진하였다. 이러한 문제점을 해결하기 위해서는 국가차원의 제도적 뒷받침이 따라야 한다. 즉 국가 R&D 성과물 관리차원에서 연구데이터를 주요 성과물로 관리해야 할 것으로 판단된다. 연구사업계획서에 DMP를 포함시키고, 연구주제 및 분야별로 데이터센터(혹은 데이터 리포지토리)를 지정하고, 국가 R&D에서 생산되는 연구데이터를 의무적으로 제출하도록 하는 것이다. 또한 데이터센터의 안정적이고 지속적인 운영을 위해 연구사업비 항목에 데이터 관리비를 신설하여 데이터센터의 운영비로 사용하도록 하면 예산문제도 어느 정도 해결 될 수 있을 것으로 본다. 또한 데이터 제출 및 인용도에 따라 데이터 생산부서 혹은 생산자에게 평가점수를 부여하는 등 보상체계 마련을 위한 연구도 필요할 것으로 보인다. 국가 R&D 연구데이터의 수집, 관리, 공유, 활용을 제대로 성공시키려면 국가 R&D 최고정책결정자의 지속적인 관심과 지원이 필수적이다.

• Journal of the Korean Association of Geographic Information Studies
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• v.22 no.3
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• pp.120-132
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• 2019

As a key infrastructure for positioning autonomous vehicles, HD maps are making efforts to equip autonomous vehicles and connect them with services worldwide. Global companies like Here and TomTom have contracted an agreement with three German automobile companies(BMW, Audi, Daimler) by establishing systems including from the production of HD maps to loading autonomous vehicles. Japan has organized a DMP(Dynamic Map Platform) by collaborating public and private section and performing establishment of HD map on private section-oriented. Korean companies related with autonomous vehicles are also cooperating with various companies such as vehicles, sensors and maps. But public and private sections are establishing HD maps respectively. Accordingly, Ministry of Land, Infrastructure and Transport implemented and signed memorandum for private and public cooperation in April 2019, Joint establishment council of HD map has launched in August 2019. This study performed domestic and foreign trend analysis about HD map and interviewed to related companies. And analyzed solutions for interactive cooperation of private and public section in HD map industry, purposed step-by-step strategy for operation and operation plan for joint establishment council of HD map.

• Journal of the korean academy of Pediatric Dentistry
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• v.46 no.2
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• pp.219-225
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• 2019

The purpose of this study was to investigate the odontoblast gene expression related to the subculture speed of supernumerary dental pulp stem cells (sDPSCs). The stem cell is undifferentiated cells which has the ability to differentiate into various cells. Specific stimulation or environment induces cell differentiation, and these differentiation leads to bone or muscle formation. 20 sDPSCs were obtained from 20 children under aseptic condition. During the culture through the 10th passage, the third passage cells which showed short subculture period and 10th passage cells which showed long subculture period were earned. Each cell was divided into differentiated group and non-differentiated group. Quantitative real-time polychain reaction (q-RT-PCR) was performed for each group. The genes related to odontoblast differentiation, Alkaline Phosphatase (ALP), Osteocalcin (OCN), Osteonectin (ONT), Dentin sialophosphoprotein (DSPP) and Dentin matrix acidic phosphoprotein 1 (DMP-1), were measured. Differentiated cells showed more gene expression levels. Undifferentiated cells showed higher gene expression level in 10th passages but differentiated cells showed higher gene expression level in 3rd passages. Cells that showed faster subculture period showed relatively lower gene expression level except for OCN and DSPP.

• Journal of the Korean Applied Science and Technology
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• v.38 no.3
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• pp.903-913
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• 2021

Three-type PVC membranes denoted by AEM-1, AEM-2, and AEM-3 with a cross-linked anion-exchange group were prepared by substitution reaction of PVC with triethyldiamine (TEDA), 1,4-dimethylpiperazine (DMP), and 1,4-bis(imidazol-1-ylmethyl)benzene (BIB) in cyclohexanone, respectively. We confirmed the successful preparation of the AEM-1, AEM-2, and AEM-3 via ionic conductivity (S/cm), water uptake (%), contact angle, ion-exchange capacity (m_eq/g), thermal properties, SEM and XPS analysis, respectively. The electrochemical capacitor experiments using PVC membrane with cross-linked anion-exchange group in organic electrolytes were performed. The prepared AEM-1, AEM-2 AEM-3 have a good stability by charge and discharge performance in organic electrolyte. As a result, the AEM-2 and AEM-3 membrane based on PVC prepared by the solvent casting method after substituent reaction is suitable for the use as a separator in organic electrochemical capacitor (supercapacitor).

• Bulletin of the Korean Chemical Society
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• v.33 no.2
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• pp.464-468
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• 2012

Three solvent systems, chlorobenzene (ink 1), chlorobenzene/o-dichlorobenzene (ink 2) and chlorobenzene/tetrahydronaphthalene (ink 3), were compared as printable inks for the fabrication of polymer light-emitting diodes (PLEDs) using poly[2-methoxy-5-(2-ethylhexyl-oxyl)-1,4-phenylenevinylene (MEH-PPV) as an emissive material and an inkjet printer (Fujifilm Dimatix DMP-2831). Ink 1 clogged the printer's nozzle and gave non-uniform film. Inks 2 and 3 were used to fabricate PLEDs with ITO/PEDOT:PSS/MEH-PPV/LiF/Al configurations. The best performance (turn-on voltage, 3.5 V; luminance efficiency, 0.17 cd/A; luminance, 1,800 cd/m) was obtained when ink 3 was used to form the emissive layer (thickness, 49 nm), attributable to the better morphology and suitable thickness of the MEH-PPV layer.

• Journal of Acupuncture Research
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• v.29 no.6
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• pp.57-71
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• 2012

Objectives : The purpose of this study is to find the effects of Sa-Am stomach tonifying acupuncture on radial pulse through the parameters difference. Methods : Forty healthy subjects participated in this study, acupuncture group and control group were divided. Radial pulse were measured using 3 dimensional pulse imaging system(DMP-3000). 19 parameters that significantly changed were selected after comparing between acupuncture group and control group. Then, 19 parameters were analyzed before, immediately after, 30 minutes after and 60 minutes after acupuncture in acupucture group. Results : 1. Heart rate, pulse period, T/T1, T2/T, T4/T, T5/T, T-T4, (T-T4)/T and T4/(T-T4) significantly changed after acupuncture. 2. H1 Amplitude, H2 Amplitude, H4 Amplitude and H5 Amplitude significantly changed after acupuncture. 3. Area of Pulse Wave, Systolic Pulse Area, Diastolic Pulse Area and Area of W significantly changed after acupuncture. 4. AIx/HR significantly changed after acupuncture. Conclusion : Stomach Tonifying Acupuncture exerts an influence on radial pulse parameters. Further radial pulse change study on various acupuncture treatment is required.

• Journal of the Korean Society for Precision Engineering
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• v.31 no.8
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• pp.737-742
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• 2014

This paper reports a customized silver ink and its inkjet printing process on a cellulose electro-active paper (EAPap). To synthesize a silver ink, silver nanoparticle is synthesized from silver nitrate, polyvinylpyrrolidone and ethylene glycol, followed by adding a viscosifier, hydroxyethyl-cellulose solution, and a surfactant, diethylene glycol. The silver ink is used in an inkjet printer (Fujifilm Dimatix DMP-2800 series) to print silver electrodes on cellulose EAPap. After printing, the electrodes are heat treated at $200^{\circ}C$. The sintered electrodes show that the thickness of the electrodes linearly increases as the number of printing layers increases. The electrical resistivity of the printed electrodes is $23.5{\mu}{\Omega}-cm$. This customized ink can be used in inkjet printer to print complex electrode patterns on cellulose EAPap to fabricate flexible smart actuators, flexible electronics and sensors.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.403.3-404
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• 2002

Dimethyl phthalate(DMP). diethyl phthalate(DEP), di-n-butyl phthalate (DBP). butyl benzyl phthalate(BBP). bis(2-ethylhexyl)phthalate(DEHP) and di-n-octyl phthalate(DOP) in lotions was determined by gas chromatography. and benzyl benzoate was used as the intermal standard. The separation of the six phthalates and internal standard was optimized, and the optimal analytical conditions were as follows: column. DB-1701 (I.D. 0.25mm): mobile phase. helium: oven temperature 20$0^{\circ}C$ (10 min) ${\rightarrow}10^{\circ}C$/min ${\rightarrow}260^{\circ}C$/min(30min), injector temperature 23$0^{\circ}C$, detector temperature 28$0^{\circ}C$. (omitted)