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Effects of Relative Lysyl Oxidase and Hydrogen Peroxide on Odontoblastic Differentiation  

Lee, Hwa-Jeong (Department of Maxillofacial Tissue Regeneration, School of Dentistry, Kyung Hee University)
Publication Information
Journal of dental hygiene science / v.13, no.3, 2013 , pp. 321-329 More about this Journal
Abstract
Although it has been reported that lysyl oxidase (LOX) is involved in odontoblastic differentiation, the role of LOX on odontoblastic differentiation by hydrogen peroxide ($H_2O_2$) have not been clarified. In the present study, we investigated whether $H_2O_2$, reactive oxygen species (ROS), is modulated the messenger RNA (mRNA) expression and activity of LOX during odontoblastic differentiation of human dental pulp (HDP) cells. The mRNA expression was quantified by reverse transcriptase polymerase chain reaction (RT-PCR) analysis, and LOX enzyme activity was measured by high sensitive fluorescent assay. Expression of the odontoblastic differentiation marker genes were assessed in the presence and absence of specific small interfering RNAs (siRNAs) of the LOX and LOXL. The $H_2O_2$-induced mRNA expression of LOX family was significant reduction of LOX, LOXL, and LOXL3 mRNA levels in HDP cells. LOX enzyme activity was increased at $H_2O_2$ 0.3 mM for 24 hours. The mRNA expression of alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCN) was inhibited by LOX- and LOXL-specific siRNAs whereas the mRNA expression of dentin matrix protein1 (DMP1), and dentin sialophosphoprotein (DSPP) was inhibited by LOX-specific siRNA. In LOX enzyme activity, siRNA-induced knockdown of both LOX and LOXL inhibited the total amine oxidase activity in HDP cells, as in the case of mRNA expression. In conclusion, the essential role of $H_2O_2$ on odontoblastic differentiation suggests that its regulation by LOX may have pharmacologic importance in HDP cells.
Keywords
Human dental pulp cells; Hydrogen peroxide; Lysyl oxidase; Odontoblastic differentiation; Small interfering RNA;
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1 Sulieman M, Addy M, Macdonald E, Rees JS: The bleaching depth of a 35% hydrogen peroxide based in-office product: a study in vitro. J Dent 33: 33-40, 2005.   DOI
2 Luk K, Tam L, Hubert M: Effect of light energy on peroxide tooth bleaching. J Am Dent Assoc 135: 194-201, 2004.   DOI
3 Benetti AR, Valera MC, Mancini MN, Miranda CB, Balducci I: In vitro penetration of bleaching agents into the pulp chamber. Int Endod J 37: 120-124, 2004.   DOI
4 Gokay O, Mujdeci A, Algn E: Peroxide penetration into the pulp from whitening strips. J Endod 30: 887-889, 2004.   DOI
5 Butler WT, Ritchie H: The nature and functional significance of dentin extracellular matrix proteins. Int J Dev Biol 39: 169-179, 1995.
6 Lee DH, Lim BS, Lee YK, Yang HC: Effects of hydrogen peroxide (H2O2) on alkaline phosphatase activity and matrix mineralization of odontoblast and osteoblast cell lines. Cell Biol Toxicol 22: 39-46, 2006.   DOI
7 Min KS, Lee HJ, Kim SH, et al.: Hydrogen peroxide induces heme oxygenase-1 and dentin sialophosphoprotein mRNA in human pulp cells. J Endod 34: 983-989, 2008.   DOI
8 Alliot-Licht B, Bluteau G, Magne D, et al.: Dexamethasone stimulates differentiation of odontoblast-like cells in human dental pulp cultures. Cell Tissue Res 321: 391-400, 2005.   DOI
9 Couble ML, Farges JC, Bleicher F, Perrat-Mabillon B, Boudeulle M, Magloire H: Odontoblast differentiation of human dental pulp cells in explant cultures. Calcif Tissue Int 66: 129-138, 2000.   DOI
10 Ruch JV, Lesot H, Bègue-Kirn C: Odontoblast differentiation. Int J Dev Biol 39: 51-68, 1995.
11 Ibaraki K, Termine JD, Whitson SW, Young MF: Bone matrix mRNA expression in differentiating fetal bovine osteoblasts. J Bone Miner Res 7: 743-754, 1992.
12 Rodan GA, Heath JK, Yoon K, Noda M, Rodan SB: Diversity of the osteoblastic phenotype. Ciba Found Symp 136: 78-91, 1988.
13 Weinreb M, Shinar D, Rodan GA: Different pattern of alkaline phosphatase, osteopontin, and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res 5: 831-842, 1990.
14 Kagan HM, Trackman PC: Properties and function of lysyl oxidase. Am J Respir Cell Mol Biol 5: 206-210, 1991.   DOI
15 Beck GR Jr, Zerler B, Moran E: Phosphate is a specific signal for induction of osteopontin gene expression. Proc Natl Acad Sci U S A 97: 8352-8357, 2000.   DOI
16 Nagaoka I, Igarashi M, Sakamoto K: Biological activities of glucosamine and its related substances. Adv Food Nutr Res 65: 337-352, 2012.   DOI
17 Kim EC, Lee HJ, Kim Y: Lysyl oxidase and the lysyl oxidase-like protein modulate odontoblastic differentiation of human dental pulp cells. J Endod 38: 769-773, 2012.   DOI
18 Kagan HM, Li W: Lysyl oxidase: properties, specificity, and biological roles inside and outside of the cell. J Cell Biochem 88: 660-672, 2003.   DOI
19 Payne SL, Fogelgren B, Hess AR, et al.: Lysyl oxidase regulates breast cancer cell migration and adhesion through a hydrogen peroxide-mediated mechanism. Cancer Res 65: 11429-11436, 2005.   DOI
20 Saito H, Papaconstantinou J, Sato H, Goldstein S: Regulation of a novel gene encoding a lysyl oxidase-related protein in cellular adhesion and senescence. J Biol Chem 272: 8157-8160, 1997.   DOI
21 Min KS, Kwon YY, Lee HJ, et al.: Effects of proinflammatory cytokines on the expression of mineralization markers and heme oxygenase-1 in human pulp cells. J Endod 32: 39-43, 2006.   DOI
22 Chang SW, Lee SI, Bae WJ, et al.: Heat stress activates interleukin-8 and the antioxidant system via Nrf2 pathways in human dental pulp cells. J Endod 35: 1222-1228, 2009.   DOI
23 Tang SS, Trackman PC, Kagan HM: Reaction of aortic lysyl oxidase with beta-aminopropionitrile. J Biol Chem 258: 4331-4338, 1983.
24 Wei X, Ling J, Wu L, Liu L, Xiao Y: Expression of mineralization markers in dental pulp cells. J Endod 33: 703-708, 2007.   DOI
25 Min KS, Lee YM, Hong SO, Kim EC: Simvastatin promotes odontoblastic differentiation and expression of angiogenic factors via heme oxygenase-1 in primary cultured human dental pulp cells. J Endod 36: 447-452, 2010.   DOI
26 Palamakumbura AH, Trackman PC: A fluorometric assay for detection of lysyl oxidase enzyme activity in biological samples. Anal Biochem 300: 245-251, 2002.   DOI
27 Hannon GJ, Rossi JJ: Unlocking the potential of the human genome with RNA interference. Nature 431: 371-378, 2004.   DOI
28 Shackelford RE, Kaufmann WK, Paules RS: Oxidative stress and cell cycle checkpoint function. Free Radic Biol Med 28: 1387-1404, 2000.   DOI
29 Ames BN, Shigenaga MK: Oxidants are a major contributor to aging. Ann N Y Acad Sci 663: 85-96, 1992.   DOI
30 Allen RG, Tresini M: Oxidative stress and gene regulation. Free Radic Biol Med 28: 463-499, 2000.   DOI
31 Pischon N, Darbois LM, Palamakumbura AH, Kessler E, Trackman PC: Regulation of collagen deposition and lysyl oxidase by tumor necrosis factor-alpha in osteoblasts. J Biol Chem 279: 30060-30065, 2004.   DOI
32 Rodriguez C, Alcudia JF, Martanez-Gonzalez J, Raposo B, Navarro MA, Badimon L: Lysyl oxidase (LOX) downregulation by TNFalpha: a new mechanism underlying TNFalpha-induced endothelial dysfunction. Atherosclerosis 196: 558-564, 2008.
33 Atsawasuwan P, Mochida Y, Parisuthiman D, Yamauchi M: Expression of lysyl oxidase isoforms in MC3T3-E1 osteoblastic cells. Biochem Biophys Res Commun 327: 1042-1046, 2005.   DOI