• Molecules and Cells
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• v.42 no.12
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• pp.840-849
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• 2019

The spatiotemporal mitotic processes are controlled qualitatively by phosphorylation and qualitatively by ubiquitination. Although the SKP1-CUL1-F-box protein (SCF) complex and the anaphase-promoting complex/cyclosome (APC/C) mainly mediate ubiquitin-dependent proteolysis of mitotic regulators, the E3 ligase for a large portion of mitotic proteins has yet to be identified. Here, we report c-Cbl as an E3 ligase that degrades DDA3, a protein involved in spindle dynamics. Depletion of c-Cbl led to increased DDA3 protein levels, resulting in increased recruitment of Kif2a to the mitotic spindle, a concomitant reduction in spindle formation, and chromosome alignment defects. Furthermore, c-Cbl depletion induced centrosome over-duplication and centriole amplification. Therefore, we concluded that c-Cbl controls spindle dynamics and centriole duplication through its E3 ligase activity against DDA3.

• Archives of Pharmacal Research
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• v.27 no.3
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• pp.351-356
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• 2004

A transdermal preparation containing diclofenac diethylammonium (DDA) was developed using an O/W microemulsion system. Of the oils tested, lauryl alcohol was chosen as the oil phase of the microemulsion, as it showed a good solubilizing capacity and excellent skin permeation rate of the drug. Pseudoternary phase diagrams were constructed to obtain the concentration range of oil, surfactant and cosurfactant for microemulsion formation, and the effect of these additives on skin permeation of DDA was evaluated with excised rat skins. The optimum formulation of the microemulsion consisted of 1.16% of DDA, 5% of lauryl alcohol, 60% of water in combination with the 34.54% of Labrasol (surfactant)/ethanol (cosurfactant) (1:2). The efficiency of formulation in the percutaneous absorption of DDA was dependent upon the contents of water and lauryl alcohol as well as Labrasol: ethanol mixing ratio. It was concluded that the percutaneous absorption of DDA from microemulsions was enhanced with increasing the lauryl alcohol and water contents, and with decreasing the Labrasol:ethanol mixing ratio in the formulation.

• Journal of the Korean Applied Science and Technology
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• v.20 no.3
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• pp.198-203
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• 2003

In this study, we synthesized p-hexyloxybenzaldehyde(HBA) by using p-hydroxybenzaldehyde and n-hexylchloride. p-dodecylaniline(DDA) prepared by nitration and reduction of dodecylbenzene. The schiff base, p-dodecylbenzylidene-$p^\prime$-hexyloxyaniline (DBHA) was synthesized by reaction of HBA and DDA. The color of synthetic compound was pale-brown and the yield was 62%. All the synthetic compounds were identified by TLC, FT-IR and ${^{i}H}$-NMR.In this study, we synthesized p-hexyloxybenzaldehyde(HBA) by using p-hydroxybenzaldehyde and n-hexylchloride. p-dodecylaniline(DDA) prepared by nitration and reduction of dodecylbenzene. The schiff base, p-dodecylbenzylidene-p'-hexyloxyaniline(DBHA) was synthesized by reaction of HBA and DDA. The color of synthetic compound was pale-brown and the yield was 62%. All the synthetic compounds were identified by TLC, FT-IR and $^1H$-NMR.

• Journal of Sensor Science and Technology
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• v.18 no.1
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• pp.95-101
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• 2009

A simple and new CMOS potentiostat circuit for amperometric sensor is described. To maintain a constant potential between the reference and working electrodes, only one differential difference amplifier (DDA) is needed in proposed design, while conventional potentiosatat requires at least 2 operational amplifiers and 2 resistors, or more than 3 operational amplifiers and 4 resistors for low voltage CMOS integrated potentiostat. The DDA with rail-to-rail design not only enables the full range operation to supply voltage but also provides simple potentiostat system with small hardwares and low power consumption.

• Journal of Korea Society of Industrial Information Systems
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• v.21 no.1
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• pp.61-70
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• 2016

This paper describes a new PMU(parametric measurement unit) design technique for automatic test equipment(ATE). Only one DDA(differential difference amplifier) is used to force the test signals to DUT(device under test), while conventional design uses two or more amplifiers to force test signals. Since the proposed technique does not need extra amplifiers in feedback path, the proposed PMU inherently guarantees stable operation. Moreover, to measure the response signals from DUT, proposed technique also adopted only one DDA amplifier as an IA(instrument amplifier), while conventional IA uses 3 amplifiers and several resistors. The DDA adopted two rail-to-rail differential input stages to handle full-range differential signals. Gain enhancement technique is used in folded-cascode type DDA to get open loop gain of 100 dB. Proposed PMU design enables accurate and stable operation with smaller hardware and lower power consumption. This PMU is implemented with 0.18 um CMOS process and supply voltage is 1.8 V. Input ranges for each force mode are 0.25~1.55 V at voltage force and 0.9~0.935 V at current force mode.

• Progress in Superconductivity and Cryogenics
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• v.18 no.1
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• pp.14-18
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• 2016

Organic compounds have a problem that the separation of structural isomer in the preparation process requires high energy consumption. This study proposes a new separation method of structural isomer using Magneto- Archimedes method. Firstly, the levitation height of 1, 6-DDA and 1, 10-DDA was respectively calculated by simulation of the forces acting on the particles under magnetic field, and it was indicated that they could be separated by the difference of levitation height. To confirm the phenomenon experimentally, white powders of 1, 6-DDA and 1, 10-DDA were formed into pellets, and were soaked in manganese chloride solution. Then the solution was put on the center of the cryostat of HTS bulk magnet (maximum magnetic flux density is 3T). As a result, it was confirmed that the separation of structural isomer by difference of levitation height could be possible.

• Tunnel and Underground Space
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• v.12 no.3
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• pp.158-170
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• 2002

Since the development of Discontinuous Deformation Analysis (DDA) by Shi (1984), there has been much improvement in the theory and programs. These, however, are all based on the assumption of a two-dimensional plane strain or plane stress state; and because a rock block system is a three-dimensional problem, a two-dimensional analysis has limited application. So a three-dimensional analysis is required in the design of rock slopes and underground spaces where three-dimensional discontinuities dominate stability. In this study three-dimensional DDA program is developed using the Shi's two-dimensional theory and program, and the two cases of three-dimensional block are analysed. The program is applied to one sliding-face blocks and wedge sliding and it gives the good results comparing to the exact solution. Multi-block cases will be analysed for many other application soon.

• Journal of Fisheries and Marine Sciences Education
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• v.17 no.3
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• pp.324-339
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• 2005

The WTO/DDA Negotiation on the Fisheries Subsidies started by DDA(Doha Development Agenda : 2001.11)at early 2002 has been held about 20 times until the fist half of 2005. The Negotiation will be terminated until the end of 2005 but now, it is impossible to predict the exact conclusion of the Negotiation. There is a sharp conflict of opinion on the WTO/DDA Negotiation on the Fisheries Subsidies between FFG(Fish Friends Groups : USA, New Zealand etc) and Anti-FFG(Rep. Korea, Japan etc). The Fisheries Subsidies is regulated by the WTO/DDA Negotiation that regulates on the subsidies of industrial products, fisheries products, forest products. Considering the progress of the WTO/DDA Negotiation on the Fisheries Subsidies, Guidelines for the Fisheries Subsidies Negotiation were established in Doha Ministerial Declaration at 2001 and a necessity of a special regulation on the Fisheries Subsidies has been discussed from 2002 to 2004. After june, 2004, the Negotiation was proceeding as Rep. Korea, Japan and Taiwan agreed with discussing a special regulation on the Fisheries Subsidies and At 1st, August, 2004, they reached an agreement on a necessity of a special regulation on the Fisheries Subsidies. At Feb, 2005, discussion was suspended because of presenting the joint proposal from 3 countries(Rep. Korea, Japan and Taiwan) and now discussion on the Fisheries Subsidies is giving the first consideration to classification of prohibited/non-actionable subsidies, S&D of a developing countries, small fisheries, problems of farming, grace period etc. From now on, Considering the 18th~the 20th negotiations, it is expected that discussion of fisheries subsidies regulation including property and range of prohibited/non-actionable subsidies, S&D of a developing countries, a definition of small fisheries is accelerated. In this point of view, this paper analyzed and arranged every country's proposals holding our own position and took a survey to fishermen and fisheries experts about prohibited/non-actionable subsidies. In addition, this paper intends to suggest the way of future-negotiation and provides essential data to help government's policy making through analyzing prohibited/ non-actionable subsidies, farming subsidies, S&D of a developing countries, small scale fisheries.

• Journal of Korean Society of Forest Science
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• v.98 no.4
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• pp.417-425
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• 2009

This study analyzed core issues addressed in the tentative agreement of WTO ministerial meetings held to finish WTO/DDA negotiations in Geneva in July 2008. The objectives of this study are to analyze changes in tariff reduction on timber products, and their influence on demand and supply of the items according to the modality of Non-Agricultural Market Access (NAMA) and to provide basic information for strategy formulation of our country for further WTO/DDA negotiations. The results indicate that there will not be significant changes in the tariff on sawnwood and on veneer sheets, however, the tariff on plywood need to be cut by around 50% from the applicable tariff rates of 2008 on condition that our country is in the position of developed countries. Therefore, the item of plywood is expected to be influenced greatly according to the change in tariff reduction. From the analysis of influence of tariff reduction on the demand and supply of timber products including sawnwood and wood based panels, such as plywood, particleboard, and fiberboard, the import quantities of the items are expected to be changed from 0.8% to 13.3% if our country is in the position of developed countries, however, they are expected to decline by 0.8%~44.3%, if our country is in the position of developing countries (22, coefficient for developing members).

• International Commerce and Information Review
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• v.15 no.2
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• pp.345-364
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• 2013

This paper analyzed the Trade Structure of environmental industry in Korea and some major countries in preparation for The Doha Development Agenda (DDA) Trade Liberalization Negotiation in the environmental goods. Based on 161 environmental goods list proposed by OECD, this paper examined the Trade Returns of worldwide environmental goods during the past 10 years from 2002 to 2011. The analysis showed that Korean environmental goods' International Market Share(IMS) in the world was 3.2%; trade specialization index(TSI) -0.128; Revealed Comparative Advantage(RCA) 1.002 in 2011. And Korea Export Similarity Index(ESI) to Japan was the highest rank in the world market. According to the results of investigation, it is clear that International Competitiveness of the Korean Environmental Industry was received a relatively poor evaluation compared with Korean trade scale. To strengthen the competitiveness, it is required that Korean government should make an effort to boost domestic environmental industry and promote systematically Trade Liberalization Negotiation including the enforcement of transient period for Korean environmental goods in the next DDA Negotiation.