• Title/Summary/Keyword: DAP

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Cloning and Characterization of DAP10 homologue gene from Olive Flounder, Paralichthys olivaceus

  • Park, Chan-Il;Kim, Mu-Chan;Hwang, Jee-Youn;Kim, Ki-Hyuk;Kim, Joo-Won
    • Journal of fish pathology
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    • v.19 no.3
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    • pp.227-233
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    • 2006
  • Olive flounder immunoreceptor DAP10 homologue cDNA was cloned from a peripheral blood lymphocytes (PBLs) cDNA library. The length of the olive flounder DAP10 cDNA is 473bp and it contains an open reading frame of 234bp. The predicted polypeptide sequence is 78 amino acids, consisting of a 22-amino acid leader, an 11-amino acid extracellular domain, a 21-amino acid transmembrane segment, and a 24-amino acid cytoplasmic domain. The amino acid sequence of olive flounder DAP10 has 56%, 50%, 32%, 31%, and 31% sequence identity with zebrafish DAP10, catfish DAP10, cattle DAP10, rat DAP10 and Monkey DAP10, respectively. Olive flounder DAP10 has a conserved aspartic acid in the transmembrane domain and a phophatidylinositol-3 kinase-binding site (YxxM/V) in the cytoplasmic region. Genomic organization reveals that olive flounder DAP10 comprises five exons and four introns. A phylogenetic analysis based on the deduced amino acid sequence grouped the olive flounder DAP10 with other species DAP10. In RT-PCR analysis, DAP10 transcripts were detected predominantly in PBLs, kidney, spleen and intestine.

Cloning and Expression of the dapD Gene from Brevibacterium lactofermentum in E. coli (Brevibacterium lactofermentum의 dapD 유전자의 Cloning 및 E. coli에서의 발현)

  • 김옥미;박선희;박혜경;이승언;하대중;이갑랑
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.802-805
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    • 2001
  • The dapD gene of Brevibacterium lactofermentum encoding tetrahydrodipicolinate N-succinyl transferase, one of the enzymes involved in lysine biosynthesis, was cloned by complementation of Escherichia coli dapD mutnat. The recombinant plasmid pLS1 was found to contain a 3.6 kb DNA fragment. Southern hybridization analysis confirmed that the cloned DNA fragment originated from B. lactofermentum. The data of L-lysine production showed that the B. lactofermentum dapD gene was expressed in E. coli.

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The Effects of Teachers' DAP Beliefs and Teacher Efficacy on Conflict Resolution Attitudes (보육교사의 발달에 적합한 실제(DAP)에 대한 신념 및 교사효능감이 갈등해결태도에 미치는 영향)

  • Choi, Insuk
    • Korean Journal of Childcare and Education
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    • v.8 no.2
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    • pp.81-99
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    • 2012
  • This study examined the effects of teachers' DAP beliefs and teacher efficacy on the conflict resolution attitudes of teachers. The study was based on the survey data from 128 early childhood teachers working in day care centers in the Incheon area. The valuables were all measured by teachers. The collected data were analyzed by Manova and Logistic Regression. The main results of this study were as follows. Firstly, there was a significant difference in collaborating and avoiding types of conflict resolution attitudes according to only age among demographic variables. Secondly, teachers' DAP beliefs and teacher efficacy were positively related to the collaborating type but negatively related to avoiding and competing types of conflict resolution attitudes. Finally, teachers' DAP beliefs and teacher efficacy had positive effects on cooperative conflict resolution attitudes after controling for demographic variables. Our findings indicate that teachers' DAP beliefs and teacher efficacy have effects on their conflict resolution attitudes during preschoolers' peer conflicts.

A Study of Dap-ho(塔胡) in Joseon Dynasty - Until the Early $17^{th}$ Century - (조선시대 답호(塔胡) 연구 - 17세기 전기까지 -)

  • Song, Mi-Kyung
    • Journal of the Korean Society of Costume
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    • v.59 no.10
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    • pp.51-67
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    • 2009
  • The study is about 'Dap-ho', a kind of men's coat in the early, and mid period of Joseon Dynasty. The study examined the kinds, color, and fabric of Dap-ho from "The Annals of the Joseon Dynasty". It also examined the characteristic, and the change of shape through excavated costume, and the period is limited until early 17th century. In "The Annals of the Joseon Dynasty", there is record of Dap-ho from King Sejong period to Gwang-hae-gun period. After 160 years, it appears again in King Young-jo period, and remains until King Soon-jong period. It was mentioned 168 times, from over 100 cases. There are 26 colors of Dap-ho from "The Annals of the Joseon Dynasty", and among them, green appears the most. Thin fabric, like Joo [紬], Sa[紗], and Cho[綃] was used often, and there are records of double layered, and cotton padded Dap-ho. The period when Dap-ho appears as excavated costume is almost the same as, "The Annals of the Joseon Dynasty" and disappears after the decease of Dae-ho Kang(1541-1624), and Sun-un Yun(1580~1628). After in this period, Dan-ryung, and Jik-ryung changes into double layered clothes, and Jik-ryung functioned as the underclothes of Dan-ryung, instead of Dap-ho. The excavated costumes of Hwak Kim(1572~1633), and Eung-hae Lee(1547~1626) proofs this. But Dap-ho was called 'Jun-bok', 'Dugree', Que-ja', and 'Dap-ho' until the later period of Joseon Dynasty.

Molecular Cloning and Expression of dapA, the Gene for Dihydrodipicolinate Synthetase of Corynebacterium glutamicum (Dihydrodipicolinate Synthetase를 코딩하는 Corynebacterium glutamicum의 dapA 유전자의 클로닝 및 발현)

  • 오종원;한종권;이현환;현형환;이재흥;스테판정
    • Korean Journal of Microbiology
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    • v.29 no.4
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    • pp.203-208
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    • 1991
  • The dapA-complementing gene (L-2, 3-dihydrodipicolinate synthetase: DHDP synthetase, dapA) has been cloned by using a cosmid genomic bank of Corynebacterium glutamicum JS231 that is a lysine overproducer, AEC (s-(2-aminoethyl)-L-cysteine) resistant mutant. By enzymatic deletion analysis, the DNA region complementing the escherichia coli dapA host could be confined to 4.5kb SalI-generated DNA fragment. This DNA fragment was inserted into the C. glutamicum/E. coli shuttle vector pECCG117 to construct pDHDP5812. The specific activity of DHDP synthetase detected in C. glutamicum JS231/pDHDP5812 was increased about 10 fold above that of C. glutamicum JS231. The addition of leucine during growth did not repress the expressin of dapA, and the enzyme activity was not inhibited by lysine.

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아시아의 DAP 시장

  • 한국비료공업협회
    • 비료회보
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    • s.551
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    • pp.16-23
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    • 2003
  • DAP는 인산질비료의 원료로 날로 그 중요성이 더해가고 있지만, 아시아의 DAP 시장은 무역관행이 제한적이라는 비난을 받으며 정착이 어려운 곳으로 전락했다.

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A study on developing a new self-esteem measurement test adopting DAP and drafting the direction of digitalizing measurement program of DAP (청소년 자존감 DAP 인물화 검사 개발 및 디지털화 측정 시스템 방향성 연구)

  • Woo, Sungju;Park, Chongwook
    • Journal of the HCI Society of Korea
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    • v.8 no.1
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    • pp.1-9
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    • 2013
  • This is to develop a new way of testing self-esteem by adopting DAP(Draw a Person) test and to make a platform to digitalize it for young people in the adolescent stage. This approach is to get high effectiveness of the self-esteem measurement using DAP test, including some personal inner situations which can be easily missed in the large statistical analysis. The other objective of this study is digitalize to recover limits of DAP test in the subjective rating standard. It is based on the distribution of the figure drawing expressed numerically by the anxiety index of Handler. For these two examinations, we made experiment through 4 stages with second grade middle school 73 students from July 30th to October 31th in 2009 during 4 months. Firstly, we executed 'Self Values Test' for all 73 people, and divided them into two groups; one is high self-esteem group of 36 people, the other is low self-esteem group of 37 people. Secondly, we regrouped them following D (Depression), Pd (Psychopathic Deviate), Sc (Schizophrenia) scales of MMPI; one is high self-esteem group of 7 people, the other is low self-esteem group of 13 people. Thirdly, we conducted DAP test separately for these 20 people. We intended to verify necessity and appropriateness of direction of 'Digitalizing Measurement System' by comparing and analyzing relation between DAP and Self-esteem following evaluation criteria which has similarity in 3 tests, after executing DAP to reflect peculiarity of adolescents sufficiently. We compared and analyzed result abstracted by sampling DAP test of two groups; One is high self-esteem group of 2 people, the other is low self-esteem group of 2 people; to confirm whether we can improve limitation that original psychological testing has by comparing mutual reliance of measurement test. Finally, with DAP test gained from correlations between self-esteem and melancholia following as above-mentioned steps, we discovered possibility of realization to get a concrete and individual criteria of evaluation based on Expert System as a way of enhancing accessibility in quantitative manner. 'Digitalizing Measurement Program' of DAP test suggested in this study promote results' reliability based on existing tests and measurement.

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A Comparative Study on Early Childhood Teachers' Beliefs and Instructional Activities Regarding DAP in Korea and China (한국과 중국 유아교사의 DAP 관련 신념과 교수실제의 비교연구)

  • Seo, Hyun-Ah;Chun, Hui-Young;Lee, Mi-Ran;Jwa, Seung-Hwa
    • Korean Journal of Child Studies
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    • v.31 no.6
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    • pp.135-151
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    • 2010
  • The purpose of this study was to analyze the relationship between teachers' beliefs and instructional activities in regards to the DAP (Developmentally Appropriate Practice) of Korean and Chinese early childhood teachers. A number of both teacher variables and classroom environment variables were also examined. One hundred and ninety three Korean early childhood teachers and 385 Chinese early childhood teachers responded to the TeacherQuestionnaireas issued. The results of this study were as follows. First, Korean early childhood teachers showed higher scores in terms of teachers' beliefs about DAP than DIP (Developmentally Inappropriate Practice). Chinese early childhood teachers showed higher scores for teachers' beliefs about DIP. Second, Korean teachers' scores in terms of instructional activities for DAP showed differences depending on the degree of teaching experience and the teachers' age, and the scores for instructional activities related to DIP did not show any differences in any of the teacher variables. On the other hand, Chinese teachers exhibited different scores for the instructional activities for both DAP and DIP, again depending on the amount of teaching experience, the teachers' ages, and the ages of the children they taught. Third, a relationship between teachers' beliefs and instructional activities for DAP was found to be in evidence in Korea, but not in China.

Isolation and Identifition of DAP-Auxotrophs from E. coli pRDI (E. coli pRDI에서의 DAP-영양요구성 변이주 분리 및 동정)

  • 이호자
    • Korean Journal of Microbiology
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    • v.22 no.4
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    • pp.265-269
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    • 1984
  • For the utilization as donor cells of conjugation, DAP-Auxotrophs were isolated from C. coli cells, carrying plasmid $p^{RD1}$ with(a) drug resistance makers from Pseudononas $(Km^r, \;Carb^r, \;Tc^r)$ and (b) the nif-gene group from Klebsiela. E. coli $p^{RD1}$ cells were treated with nitrosoguanidine for the mutagenesis and cephalexin for the isolation of DAP-Auxotrophs. The nature of auxotrophs was verified by suitable biochemical test and checking with 6-cyanopurine as a color indicator for the presence of nif-gene.

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A study on the design using characteristic the Joseon dynasty Dap-ho (조선시대 답호의 조형특성을 응용한 디자인 연구)

  • Yeom, Soon Jeong;Kim, Eun Jung
    • Journal of the Korea Fashion and Costume Design Association
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    • v.21 no.1
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    • pp.87-101
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    • 2019
  • The sleeve is closely connected with activity in terms of costume. As a kind of overcoat, Dap-ho of the Joseon Dynasty is short-sleeved or sleeveless. Dap-ho, with simple sleeves is convenient for layering and taking off and since it is easily adjustable using a gusset, a slit, or coat string, and this can be applied to layered look-related designs. This study aims to suggest a design item, which sustains the existence of traditional costumes and facilitates diverse layered looks and co-ordination, using the formative elements of Dap-ho. For this study, theoretical backgrounds and relics of Dap-ho of the Joseon Dynasty were analyzed, based on the previous studies, ancient literature, "The Annals of the Joseon Dynasty", and relics preserved in museums. The analysis targeted nine relics that show the birth and death years and the constructive changes in shapes, colors, materials of Dap-ho. Formative elements of Dap-ho were applied to the designing and the manufacturing of clothes for middle-aged women in their fifties and the research conclusions are as follows. First, from a morphological perspective, the silhouette, gusset, slit, and the indirectly attached coat string of Dap-ho are good to be used as various design items for covering the body shapes of middle-aged women, and the short-sleeved or sleeveless type is convenient to be layered. Second, when it comes to the material, it is possible to emphasize a traditional image and practicality at the same time by mixing and matching the Hanbok cloth and cotton. Third, in relation to the colors, the coexistence-based color arrangement method considering the theory of Yin and Yang can be applied to modern clothes and this makes it possible to express a traditional image in a harmonious way.