Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Cloning and Expression of the dapD Gene from Brevibacterium lactofermentum in E. coli

Brevibacterium lactofermentum의 dapD 유전자의 Cloning 및 E. coli에서의 발현

  • 김옥미 (대경대학 호텔쿠크리과) ;
  • 박선희 (영남대학교 식품영양학과) ;
  • 박혜경 (영남대학교 식품영양학과) ;
  • 이승언 (영남대학교 식품영양학과) ;
  • 하대중 (대경대학 호텔쿠크리과) ;
  • 이갑랑 (영남대학교 식품영양학과)
  • Published : 2001.10.01
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Abstract

The dapD gene of Brevibacterium lactofermentum encoding tetrahydrodipicolinate N-succinyl transferase, one of the enzymes involved in lysine biosynthesis, was cloned by complementation of Escherichia coli dapD mutnat. The recombinant plasmid pLS1 was found to contain a 3.6 kb DNA fragment. Southern hybridization analysis confirmed that the cloned DNA fragment originated from B. lactofermentum. The data of L-lysine production showed that the B. lactofermentum dapD gene was expressed in E. coli.

산업적으로 lysine 발효 산업에 이용되고 있는 B. lactofermentum으로부터 lysine 생합성에 관여하는 tetrahyrodipicolinate N-succinyl transferase를 지령하는 dapD 유전자를 E. coli의 dapD 결손변이주와의 complementation test를 통하여 cloning하였다. 재조합 plamid는 3.6 kb의 DNA 단편을 함유하고 있었으며 Southern blot hybridization을 통하여 dapD 유전자는 B. lactofermentum으로부터 유래하였으며 염색체 DNA내에 single copy로 존재함을 알 수 있었다. 또한 lysine 생성량 분석을 통하여 E. coli에서 B. lactofermentum dapD 유전자의 발현을 확인하였다.

Keywords

References

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