• Title/Summary/Keyword: D-band

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Studies on the VP4 and VP7 Genes of Bovine Rotaviruses from Field Samples Using RT-PCR and RFLP Analysis (RT-PCR과 RFLP법을 이용한 국내 소 로타바이러스 VP4 및 VP7 유전자의 특성 규명)

  • Jeon, Seong-Jin;Chang, Chung-Ho;Chung, Chung-Won;Kim, Won-Yong;Kang, Shien-Young
    • The Journal of Korean Society of Virology
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    • v.28 no.2
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    • pp.165-174
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    • 1998
  • Characterizations of the VP4 (P type) and VP7 (G type) genes of Korean isolates of bovine rotavirus were performed using RT-PCR/RFLP and nucleotide sequencing analysis. After RT-PCR amplification of partial length (1094bp) of the VP4 and full length (1062bp) of the VP7 genes, amplified PCR products were digested with restriction endonucleases and digestion patterns were compared with those of reference rotaviruses. With the VP4 genes, four RFLP (A-D) profiles were observed; three (A, Band C) were the same as those of bovine rotavirus NCDV (P[1]), IND (P[5]) and B223 (P[11]), respectively. Profile D was the same as that of porcine rotavirus OSU (P[7]). With the VP7 genes, five RFLP profiles (I-V) were observed; three of them (I, II and III) were the same as those of bovine rotavirus NCDV (G6), Cody 1-801 (G8), and B223 (G10), respectively. Profile IV and V were atypical to those of reference bovine rotaviruses used in this study. These two profiles were identified as G6 and G5, respectively, after analyzing and comparing the nucleotide sequences. The G typing analysis revealed that 61.9% (26/42) were G6, which included G6 subtype; 28.6% (12/42) were G5; 7.1% (3/42) were G10; 2.4% (1/42) were G8. The P typing analysis revealed that 54.8% (23/42) were P[5]; 28.6% (12/42) were P[7]; 11.8% (5/42) were [11]; 4.8% (2/42) were P[1]. Our results showed that G6/P[5] were the most prevalent rotaviruses in diarrheic calves in Korea. Also, this is the first report that G5/P[7] rotaviruses were identified from cattle with diarrhea.

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Plant Regeneration by Anther Culture of Tetraploid Populus alba L.X P.glandulosa Uyeki (4배체 현사시나무 (Populus alba L. X P. gludulosa Uyeki)의 약배양에 의한 식물체 재분화)

  • Son, Sung-Ho;Kim, Jung-Hee;Moon, Heung-Kyu;No, Eun-Woon;Lee, Yoon-Hee;Kim, Mi-Hee;Park, Jin-Sun;Lee, Yong-Wook;Yoon, Yang;Lee, Seok-Gu
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.3
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    • pp.121-126
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    • 1995
  • Diploid plants were obtained by anther culture of tetraploid poplar(Populus alba L. X P.glandutosa Uyeki). The effect 2,4D on callus formation from anther culture was greater than any other auxins tested. The highest average number of multiple shoots per callus was obtained when zeatin was used at levels of 6-8 ${\mu}$M. Regenerated shoots were excised and transferred to MS basal medium. Rooted plantlets were subsequently transferred to pots containing artificial soil mix. Finally 100 plane were transplanted in nursery located in forest Genetics Research Institute. for the 300 anther clones growing in greenhouse for 6 months after transplanting, 33% were slow-growing, 47% were rapid-growing and 20% had huge leaf size with rapid-growing characteristics. Chromosome study showed a narrow range of variation from diploid to tetraploid. DNA polymorphism studies using various RAPD markers revealed some extend of differences among the anther-clones in their band pattern.

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A UHF-band Passive Temperature Sensor Tag Chip Fabricated in $0.18-{\mu}m$ CMOS Process ($0.18-{\mu}m$ CMOS 공정으로 제작된 UHF 대역 수동형 온도 센서 태그 칩)

  • Pham, Duy-Dong;Hwang, Sang-Kyun;Chung, Jin-Yong;Lee, Jong-Wook
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.45 no.10
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    • pp.45-52
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    • 2008
  • We investigated the design of an RF-powered, wireless temperature sensor tag chip using $0.18-{\mu}m$ CMOS technology. The transponder generates its own power supply from small incident RF signal using Schottky diodes in voltage multiplier. Ambient temperature is measured using a new low-power temperature-to-voltage converter, and an 8-bit single-slope ADC converts the measured voltage to digital data. ASK demodulator and digital control are combined to identify unique transponder (ID) sent by base station for multi-transponder applications. The measurement of the temperature sensor tag chip showed a resolution of $0.64^{\circ}C/LSB$ in the range from $20^{\circ}C$ to $100^{\circ}C$, which is suitable for environmental temperature monitoring. The chip size is $1.1{\times}0.34mm^2$, and operates at clock frequency of 100 kHz while consuming $64{\mu}W$ power. The temperature sensor required a -11 dBm RF input power, supported a conversion rate of 12.5 k-samples/sec, and a maximum error of $0.5^{\circ}C$.

A Design of Wideband Frequency Synthesizer for Mobile-DTV Applications (Mobile-DTV 응용을 위한 광대역 주파수 합성기의 설계)

  • Moon, Je-Cheol;Moon, Yong
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.45 no.5
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    • pp.40-49
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    • 2008
  • A Frequency synthesizer for mobile-DTV applications is implemented using $0.18{\mu}m$ CMOS process with 1.8V supply. PMOS transistors are chosen for VCO core to reduce phase noise. The measurement result of VCO frequency range is 800MHz-1.67GHz using switchable inductors, capacitors and varactors. We use varactor bias technique for the improvement of VCO gain linearity, and the number of varactor biasing are minimized as two. VCO gain deterioration is also improved by using the varactor switching technique. The VCO gain and interval of VCO gain are maintained as low and improved using the VCO frequency calibration block. The sigma-delta modulator for fractional divider is designed by the co-simualtion method for accuracy and efficiency improvement. The VCO, PFD, CP and LF are verified by Cadence Spectre, and the sigma-delta modulator is simulated using Matlab Simulink, ModelSim and HSPICE. The power consumption of the frequency synthesizer is 18mW, and the VCO has 52.1% tuning range according to the VCO maximum output frequency. The VCO phase noise is lower than -100dBc/Hz at 1MHz at 1MHz offset for 1GHz, 1.5GHz, and 2GHz output frequencies.

A Low-Voltage Low-Power Analog Front-End IC for Neural Recording Implant Devices (체내 이식 신경 신호 기록 장치를 위한 저전압 저전력 아날로그 Front-End 집적회로)

  • Cha, Hyouk-Kyu
    • Journal of the Institute of Electronics and Information Engineers
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    • v.53 no.10
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    • pp.34-39
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    • 2016
  • A low-voltage, low-power analog front-end IC for neural recording implant devices is presented. The proposed IC consists of a low-noise neural amplifier and a programmable active bandpass filter to process neural signals residing in the band of 1 Hz to 5 kHz. The neural amplifier is based on a source-degenerated folded-cascode operational transconductance amplifier (OTA) for good noise performance while the following bandpass filter utilizes a low-power current-mirror based OTA with programmable high-pass cutoff frequencies from 1 Hz to 300 Hz and low-pass cutoff frequencies from 300 Hz to 8 kHz. The total recording analog front-end provides 53.1 dB of voltage gain, $4.68{\mu}Vrms$ of integrated input referred noise within 1 Hz to 10 kHz, and noise efficiency factor of 3.67. The IC is designed using $18-{\mu}m$ CMOS process and consumes a total of $3.2{\mu}W$ at 1-V supply voltage. The layout area of the IC is $0.19 mm^2$.

Design of the Linked Patch Monopole Antenna and Its SAR Analysis along with Antenna Direction (연결된 패치 형태의 모노폴 안테나 설계 및 안테나 탑재 방향에 따른 SAR 분석)

  • Yang, Joo-Hun;Lee, Seungwoo;Kim, Nam
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.23 no.10
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    • pp.1117-1127
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    • 2012
  • In this paper, the monopole antenna for satisfying GSM900/DCS1800/PCS1900/UMTS2100 services is designed. We can get the characteristic of the low frequency bands by connecting the front patch to the back patch of the antenna and get the low frequency resonance band using a front patch slit. The proposed antenna total volume is $40{\times}98{\times}1.6\;mm^3$, and it is designed on the FR-4 substrate having a relative dielectric constant of 4.4. As measurement result after fabrication, showed that the resonant frequency bandwidths are 156 MHz(828~984 MHz), 708 MHz(1.476~2.184 GHz) based on the return loss of 10 dB, and the radiation patterns show as the omnidirectional shapes for the E-field and H-field. For analyzing the human effects, the proposed antenna is mounted on the mobile-phone case. The averaged peak SAR over 1 g and 10 g is simulated and measured when the input power is 0.25 W. We have checked the variation of the SAR values when the antenna is mounted 4 different directions, then checked the direction having a relatively higher SAR. The results also satisfied the limiting SAR values which are 1.6 W/kg and 2.0 W/kg averaged over 1 g and 10 g tissues respectively.

Design for a Tag Antenna Using License Plate Attached Vehicle Bumper (차량 범퍼에 부착된 번호판용 태그 안테나의 설계)

  • Park, Dea-Hwan;Min, Kyeong-Sik
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.20 no.6
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    • pp.535-543
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    • 2009
  • This paper describes a design for RFID(Radio Frequency Identification) tag antenna using license plate attached the vehicle bumper in 900 MHz band. The proposed tag antenna size which is located on upper center position of a vehicle license plate is 162.5${\times}$40${\times}$1 mm$^3$. A resonant frequency of design antenna and the bandwidth which has return loss of -10 dB below are 900 MHz and about 720 MHz(640${\sim}$1,360 MHz), respectively. The commercial chip impedance considered on design was 16- j131 ${\Omega}$ and the complex conjugate impedance of chip was used as input impedance of tag antenna. The measured return loss and radiation pattern were agreed well with the calculated results. The measured readable range of the proposed tag antenna designed on only the vehicle license plate was 11.5 m. Moreover, its range of the fabricated tag antenna that the license plate and the vehicle bumper were fixed by volt and nut was observed 10.4 m. These measured readable range showed about 5 m above far distance more than the average readable range of commercial tag antenna.

Avoidance of Internal Resonances in Hemispherical Resonator Assemblies from Fused Quartz Connected by Indium Solder

  • Sarapuloff, Sergii A.;Rhee, Huinam;Park, Sang-Jin
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2013.04a
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    • pp.835-841
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    • 2013
  • Modern solid-state gyroscopes (HRG) with hemispherical resonators from high-purity quartz glass and special surface superfinishing and ultrathin gold coating become the best instruments for precise-grade inertial reference units (IRU) targeting long-term space missions. Designing of these sensors could be a notable contribution into development of Korea as a space nation. In participial, 40mm diameter thin-shell resonator from high-purity fused quartz, fabricated as a single-piece with its supporting stem has been designed, machined, etched, tuned, tested, and delivered by STM Co. (ATS of Ukraine) several years ago; an extremely-high Q-factor (upto 10~20 millions) has been shown. Understanding of the best way how to match such a unique sensor with inner glass assembly of the gyro means how to use the high potential in a maximal extent; and this has become the urgent task. Inner quartz glass assembly has a very thin indium (In) layer soldered the resonator and its silica base (case), but effects of internal resonances between operational modal pair of the shell-cup and its side (parasitic) modes can notable degrade the potential of the sensor as a whole, instead of so low level of resonator's intrinsic losses. Unfortunately, there are special combinations of dimensions of the parts (so-called, "resonant sizes"), when intensive losses of energy occurs. The authors proposed to use the length of stem's fixture as an additional design parameter to avoid such cases. So-called, a cyclic scheme of finite element method (FEM) and ANSYS software were employed to estimate different combinations of gyro assembly parameters. This variant has no mismatches of numerical origin due to FEM's discrete mesh. The optimum length and dangerous "resonant lengths" have been found. The special attention has been paid to analyses of 3D effects in a cup-stem transient zone, including determination of a difference between the positions of geometrical Pole of the resonant hemisphere and of its "dynamical Pole", i.e., its real zone of oscillation node. Boundary effects between the shell (cup) and 3D short "beams" (inner and outer stems) have been ranged. The results of the numerical experiments have been compared with the classic model of a quasi-hemispherical shell band with inextensional midsurface, and the solution using Rayleigh's functions of the $1^{st}$ and $2^{nd}$ kinds. To guarantee the truth of the recommended sizes to a designer of the real device, the analytical and FEM results have been compared with experimental data for a party of real resonators. The consistency of the results obtained by different means has been shown with errors less than 5%. The results notably differ from the data published earlier by different researchers.

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Different Physiological Activity of Selected Rice Cultivars to Diphenylether Herbicide, Oxyfluorfen - VI. Responses of Oxyfluorfen - Similar Herbicides (Oxyfluorfen에 대한 내성(耐性) 및 감수성(感受性) 벼품종(品種)의 생리활성(生理活性) 기구(機構) - VI. 유사제초제(類似除草劑)에 대한 반응(反應))

  • Kuk, Y.I.;Guh, J.O.
    • Korean Journal of Weed Science
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    • v.16 no.4
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    • pp.337-345
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    • 1996
  • This study was conducted to investigate the protox activity, the PPIX accumulation and the activity of antioxidative enzymes of the oxyfluorfen-tolerant and -susceptible rice cultivars by oxyfluorfen and oxytluorfen-similar herbicides treatment. When treated with acifluorfen, bifenox or oxadiazon, the oxyfluorfen-tolerant rice cultivars showed less decreased in fresh weight than the susceptible rice cultivars. The inhibition of protox activity was in the order of acifluorfen > oxyfluorfen > bifenox > oxadiazon, and the PPIX accumulation was in the sequence of oxadiazon > acifluorfen > oxyfluorfen > bifenox. The inhibition of protox activity and the PPIX accumulation by the herbicide was greater in the susceptible rice cultivars than in the tolerant rice cultivars. The effect inhibiting the decrease of chlorophyll content resulting from the treatment of GC, tetrapyrrole biosynthesis inhibitor, was in the order of oxyfluorfen > acifluorfen > bifenox > oxadiazon, and the tolerant rice cultivar had more than the susceptible rice cultivar. In the treatment of DPE and oxadiazon, the activities of MDAR, POX and GR was higher in the tolerant rice cultivar than in the susceptible rice cultivar, and in the case of POX isozyme the activities of main D and E bands increased in the tolerant rice cultivars at tested herbicides but they didn't in the susceptible rice cultivar.

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A Case Report for a Korean Patient with Mucopolysaccharidosis IIIA Confirmed by Biochemical and Molecular Genetic Investigation (생화학적 검사 및 분자유전학적 검사에 의해 뮤코다당증 제3A형으로 진단된 한국인 환자의 증례 보고)

  • Kim, Borahm;Cho, Sung Yoon;Sohn, Young Bae;Park, Hyung-Doo;Lee, Soo-Youn;Song, Junghan;Jin, Dong-Kyu
    • Journal of The Korean Society of Inherited Metabolic disease
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    • v.15 no.1
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    • pp.44-48
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    • 2015
  • Mucopolysaccharidosis (MPS) IIIA is a lysosomal storage disorder caused by abnormalities of the enzyme Heparan N-sulfatase that is required for degradation of heparan sulfate. The patient in this study was a 5 year-old boy who presented with macrocephaly and developmental delay. Urinary excretion of glycosaminoglycan was increased (26 g/moL creatinine, reference range: <7 g/moL creatinine) and a distinct band of heparan sulfate was shown in electrophoresis. Heparan N-sulfatase activity was significantly decreased in skin fibroblasts (0.2 pmoL/min/mg protein, reference range: 9-64 pmoL/min/mg protein). PCR and direct sequencing analysis of the SGSH gene showed compound heterozygous mutations: c.1040C>T (p.S347F) and c.703G>A (p.D235N). This is the first report for a Korean patient with MPS IIIA who was confirmed by biochemical investigation and molecular genetic analyses.