• Journal of Radiation Industry
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• v.5 no.1
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• pp.47-54
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• 2011

In this study, we investigated to evaluate differential expression of genes encoding lipid transfer proteins (LTP) by acute and chronic gamma irradiation in rice. After acute and chronic gamma irradiation by 100 Gy and 400 Gy to rice plant, necrotic lesion was observed in the leaf blade and anthocyanin contents were increased. We isolated a total of 21 rice lipid transfer protein (LTP) genes in the TIGR database, and these genes were divided into four different groups on the basis of nucleotide sequences. The LTP genes also were classified as different four classes according to expression pattern using RT-PCR. Group A, B contained genes with increased expression and decreased expression in acute and chronic, respectively. Group C contained genes with contrasted expression pattern. Group D wasn't a regular pattern. But the specific affinity was not obtained between two grouping.

• Journal of the Korea Convergence Society
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• v.10 no.11
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• pp.85-93
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• 2019

Despite its matchless position in the global semiconductor industry, Korea has not distinguished itself in the semiconductor equipment sector. Semiconductor cleaning equipment is one of the semiconductor fabrication equipment, and it is expected to be more important along with the advancement of semiconductor fabrication processes. This study attempts to analyze technology competitiveness of major countries in the sector including Korea, and explore specialty sub-areas of the countries. For this purpose, we collected patents of semiconductor cleaning equipment during the last 10 years from the US patent database, and implemented quantitative patent analysis and co-classification network analysis. The result shows that, the US and Japan have been leading the technological progress in this sector, and Korea's competitiveness has lagged behind not only the leading countries but also its competitors and even latecomers. Therefore, intensive R&D and developing technological capabilities are needed for advancing the country's competitiveness in the sector.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.43-43
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• 2015

천수방정식을 사용하는 초기 수치모형은 프로드수($F_4$)가 변화하는 흐름 즉, 상류방향과 하류방향으로 전파하는 홍수파를 동시에 해석하기 위해 중앙 차분기법이 필요한 상류(sub-critical flow)와 흐름방향에 따른 상류이송(upwinding)기법이 필요한 사류(super-critical flow)가 나타나는 흐름해석에서 어려움이 있었다. 하지만, 근사 Riemann 해법의 등장으로 흐름방향에 관계없이 특성선을 따라 정확한 상향가중기법의 적용이 가능하게 되어, 천수방정식을 지배방정식으로 하는 수치모형이 더욱 실용적으로 적용될 수 있도록 하였다. 따라서, 현재 근사 Riemann 해법은 Godunov 형 유한체적 기법, 불연속 Galerkin 혹은 Petrov-Galerkin 유한요소기법 그리고 Boussinesq 기법에도 적용되고 있으며, 특히 Godunov 형 유한체적기법과 결합한 근사 Riemann 해법은 댐 붕괴, 하천 범람 그리고 도시 및 해안지역 침수에 이르기까지 여러 가지 문제에 폭넓게 적용되고 있다. 지금까지 홍수 모델링에 적용된 Godunov형 유한체적모형은 정형 사각격자나 비정형 삼각격자 중에서 한가지의 격자 종류만을 적용한 연구가 주로 수행되었으며, 유한요소모형과 같이 이 두 가지 격자를 동시에 적용한 연구는 거의 이루어지지 않고 있다. 일반적으로, 삼각격자는 사각격자와 는 달리 연구유역의 경계나 지형이 복잡한 경우에도 큰 노력없이 격자의 생성이 가능하나, 격자와 노드의 수가 사각격자보다 많아 계산시간이 많이 소요되는 단점이 있다. 반면, 사각격자는 하천과 같이 선형으로 변하는 지형에 대해서는 표현하기가 용이하며 계산시간의 효율성도 뛰어나다. 본 연구에서는 하천, 도시 그리고 해안지역에서의 효율적이고 정확한 홍수 모델링을 위해 삼각 및 사각격자 그리고 이 두 격자를 동시에 고려한 하이브리드 격자의 적용이 가능한 Godunov형 2차원 유한체적 모형을 개발하였다. 그리고 개발모형을 정확해가 있는 댐 붕괴 문제, 실측치가 존재하는 실험하도 및 실제하도에 삼각, 사각 그리고 혼합격자를 생성하여 모의를 수행하고, 각 적용 격자에 따른 정확성과 효율성 및 장점과 단점을 연구하였다.

• PNF and Movement
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• v.1 no.1
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• pp.43-57
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• 2003

목적: 병원치료가 필요하지 않을 만큼의 경부통증/증상과 관절가동범위, 경흉추의 다각적 면에서 관계를 조사하기 위해서이다. 방법: 연령은 19세에서 42세(평균연령 28세로 실험에 참여하기를 원하는 건강한 성인 40명을 대상으로 하였다. 경흉추의 척추 자세, 경추 능동 관절 가동범위, 경부 분절 길이 등의 다각적인 면을 측정하였다. 모든 측정들은 동일시에 각 대상자에게 서로 다른 측정자에 의해 두 번 실시되었다. 경부 근 지구력은 수정된 Biering-Sorensen 검사법에 의해 측정되었다. 마지막으로 대상자들에게 경부 통증/증상의 재발에 대한 질문을 하였다. 결과: 14명의 대상자들은 경미한 재발성 경부 통증/증상을 보고하였다. 경부 근 지구력 시간(F(1,38)=6.75, p=0.01)과 좌측 회전 가동 범위(F(1,38)=4.56, p=0.04)가 경부 통증을 가진 대상자들에서 유의하게 감소하였다. 신전 가동범위가 재 측정에서 특정군 변화 즉, 정상군 증가, 경부 통증/증상군 감소(F(1,38)=4.67, p=0.04)가 보였다. 경부 통증/증상군은 정상군과 비교 시 후인 가동 범위의 증가하였다(F(1,38) =4.56, p=0.04). 통증 유무에 관계없이 모든 대상자들에서 우측 회전보다 좌측 회전에서 가동범위가 더 크게 나타났고(F(1,38) =4.34, p=0.04), 반복 측정에서 좌측 측방굴곡 (F(1,38) =5.10, p=0.03)과 우측 측방 굴곡(F(1,38) = 5.27, p=0.03)의 감소가 나타났다. 결론: 경미한 증상의 경부통증 대상자군과 정상적인 대상자군을 비교할 때 그룹 간 차이는 경부 근 지구력 시간의 감소, 좌측 회전 가동 범위 감소와 특히, 두 번 째 측정에서 신전범위 감소가 나타났으나 후인의 가동범위는 증가로 관찰되었다. 이러한 결과들은 경부 통증의 발생과 관련된 초기 가동범위 변화를 제안한다.

• The Korean Journal of Food And Nutrition
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• v.34 no.2
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• pp.196-206
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• 2021

The main purpose of this study was to examine the correlation between the consumption of red ginseng-based 'SSR' for 30 days and the reduction in human fatigue, blood component changes, and immune cell activity in 35 human subjects. 'SSR' is composed of zinc oxide, folic acid, and D-α-tocopherol with red ginseng as the main component. According to the protocol criteria of the study, 35 subjects who understood the purpose of the study and signed an informed consent form were selected. The fatigue survey was conducted through a questionnaire, and after taking 'SSR', a decreased tendency of physical, mental, and neurosensory fatigue was observed. In hematological analysis, no significant changes were observed in the levels of WBC, RBC, and hemoglobin; however, AST (SGOT) and ALT (SGPT) levels were statistically significantly decreased. In immunological analysis, it was observed that the proliferative effect of T cells (CD3+CD4+) was greater than that of NK cells (CD16+CD56+). The collected data were subjected to t-test analysis using the SPSS 25.0 statistical program. The result from this study proposes that 'SSR' can be used as a functional food material as it reduces human fatigue and enhances immune function.

• Toxicological Research
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• v.35 no.1
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• pp.13-24
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• 2019

Numerous ethnomedicinal uses have been attributed to different parts of Annona senegalensis (ASE), including its uses as food and food additives. The present study investigated toxicological and antioxidant effects of 28 days administration of ethanol extracts of ASE stem bark to Wistar strain albino rats. Acute toxicity test was done to determine lethal dose in Wistar rats while sub-acute toxicity test was conducted on rats divided into four groups (A - control, B - 50 mg/kg, C - 100 mg/kg, D - 150 mg/kg, respectively and treated for 28 days. Oxidative stress markers in liver and kidney as well as hepatic succinate dehydrogenase activity in the mitochondrial and post mitochondrial fractions (PMF) were evaluated. The $LD_{50}$ value of ASE was > 2,000 mg/kg. White blood cell counts gradually increased, but red blood cell counts and haematocrits level decreased significantly (p < 0.05) by about 50%. Liver enzymes in the serum and mitochondrial succinate dehydrogenase activity increased significantly (p < 0.05). Superoxide dismutase and catalase activities also increased in liver mitochondria and PMF while malondialdehyde (MDA) and reduced glutathione levels increased only in the PMF. Furthermore, only MDA levels increased significantly in the kidney after 28 days extract administration. Histopathological examination showed hepatic necrosis and no obvious signs of nephrotoxicity. Anona senegalensis is relatively safe, but prolonged ingestion could induce oxidative stress and impair ATP synthesis through the modulation of the activity of mitochondrial succinate dehydrogenase.

• Journal of the Korea Society for Simulation
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• v.27 no.4
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• pp.69-77
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• 2018

Launching system is designed to store the payload, withstand the rigors, and prevent it from rusting and damaging. The behavior during initial deployment of the missile is determined by production, assembly and insertion condition of a launching tube and a missile. The purpose of this research is to confirm the safety of a launching tube by statistically analyzing behavior of the missile, during initial deployment stage. Error parameters which effect initial behavior of the missile are selected and analyzed through Monte-Carlo Simulation. Based on the result of simulation, tip-off and stress distribution between rail and shoe is predicted by using the commercial analysis program called Recurdyn. Lastly, the safety factor is calculated based on yield strength of the material and maximum stress of the rail during the process of launching. The safety of the launching system is verified from the result of the safety factors.

• BioChip Journal
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• v.12 no.4
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• pp.332-339
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• 2018

The future neuro-prosthetic devices would be required spikes data monitoring through sub-nanoscale transistors that enables to neuroscientists and clinicals for scalable, wireless and implantable applications. This research investigates the spikes monitoring through integrated CNT front-end amplifier for neuro-prosthetic diagnosis. The proposed carbon nanotube-based architecture consists of front-end amplifier (FEA), integrate fire neuron and pseudo resistor technique that observed high electrical performance through neural activity. A pseudo resistor technique ensures large input impedance for integrated FEA by compensating the input leakage current. While carbon nanotube based FEA provides low-voltage operation with directly impacts on the power consumption and also give detector size that demonstrates fidelity of the neural signals. The observed neural activity shows amplitude of spiking in terms of action potential up to $80{\mu}V$ while local field potentials up to 40 mV by using proposed architecture. This fully integrated architecture is implemented in Analog cadence virtuoso using design kit of CNT process. The fabricated chip consumes less power consumption of $2{\mu}W$ under the supply voltage of 0.7 V. The experimental and simulated results of the integrated FEA achieves $60G{\Omega}$ of input impedance and input referred noise of $8.5nv/{\sqrt{Hz}}$ over the wide bandwidth. Moreover, measured gain of the amplifier achieves 75 dB midband from range of 1 KHz to 35 KHz. The proposed research provides refreshing neural recording data through nanotube integrated circuit and which could be beneficial for the next generation neuroscientists.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.32 no.3
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• pp.1-12
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• 2019

Objectives: Amygdalin is abundant in the seeds of bitter almond and apricots of the Prunus genus and other rosaceous plants. Amygdalin is known to have antitussive and anticancer activities. Apoptosis, also known as programmed cell death, is an important mechanism in cancer treatment. Methods: In the present study, we investigated whether the aqueous extract of Amygdalin induces apoptotic cell death in ME-180 cervical cancer cells. For this study, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, terminal deoxynuclotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, flow cytometric analysis, DNA fragmentation assay, Western blot, and caspase-3 enzyme assay were performed on ME-180 cervical cancer cells. Results: Through morphological and biochemical analyses, it was demonstrated that ME-180 cells treated with Amygdalin exhibit several apoptotic features. The treatment of Amygdalin increased the Bax expression and caspase-3 enzyme activity and decreased Bcl-2 expression. Here, we have shown that Amygdalin induces apoptotic cell death in ME-180 cervical cancer cells through Bax-dependent caspase-3 activation. These results suggest the possibility that Amygdalin exerts anti-tumor effect on human cervical cancer.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.3
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• pp.350-356
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• 2019

Objective: To examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide. Methods: The mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skeletal muscle tissue was confirmed using quantitative-reverse transcriptase polymerase chain reactions (qRT-PCR). We then extracted horse muscle cells from the skeletal muscle tissue of a neonatal Thoroughbred. Myokine expression after hydrogen peroxide treatments was confirmed using qRT-PCR in horse skeletal muscle cells. Results: IL-6, IL-8, CXCL2, and CCL4 expression in Thoroughbred and Jeju horse skeletal muscles significantly increased after exercise. We stably maintained horse skeletal muscle cells in culture and confirmed the expression of the myogenic marker, myoblast determination protein (MyoD). Moreover, myokine expression was validated using hydrogen peroxide ($H_2O_2$)-treated horse skeletal muscle cells. The patterns of myokine expression in muscle cells were found to be similar to those observed in skeletal muscle tissue. Conclusion: We confirmed that several myokines involved in inflammation were induced by exercise in horse skeletal muscle tissue. In addition, we successfully cultured horse skeletal muscle cells and established an in vitro system to validate associated gene expression and function. This study will provide a valuable system for studying the function of exercise-related genes in the future.