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http://dx.doi.org/10.6114/jkood.2019.32.3.001

Anti-tumor Effect of Amygdalin extracted from Armeniacae Amarum Semen on Human Cervical Cancer Cell ME-180  

Choi, Yong-Seok (Dept. of Anatomy-Pointology, College of Korean Medicine, Gachon University)
Kim, Youn-Sub (Dept. of Anatomy-Pointology, College of Korean Medicine, Gachon University)
Kim, Gyung-Jun (Dept. of Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Gachon University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.32, no.3, 2019 , pp. 1-12 More about this Journal
Abstract
Objectives: Amygdalin is abundant in the seeds of bitter almond and apricots of the Prunus genus and other rosaceous plants. Amygdalin is known to have antitussive and anticancer activities. Apoptosis, also known as programmed cell death, is an important mechanism in cancer treatment. Methods: In the present study, we investigated whether the aqueous extract of Amygdalin induces apoptotic cell death in ME-180 cervical cancer cells. For this study, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, terminal deoxynuclotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, flow cytometric analysis, DNA fragmentation assay, Western blot, and caspase-3 enzyme assay were performed on ME-180 cervical cancer cells. Results: Through morphological and biochemical analyses, it was demonstrated that ME-180 cells treated with Amygdalin exhibit several apoptotic features. The treatment of Amygdalin increased the Bax expression and caspase-3 enzyme activity and decreased Bcl-2 expression. Here, we have shown that Amygdalin induces apoptotic cell death in ME-180 cervical cancer cells through Bax-dependent caspase-3 activation. These results suggest the possibility that Amygdalin exerts anti-tumor effect on human cervical cancer.
Keywords
Amygdalin; Cervical Cancer; Apoptosis; Bcl-2; Caspase-3; Bax;
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