Differential Expression of Rice Lipid Transfer Protein Gene (LTP) Classes in Response to γ-irradiation Pattern

감마선 조사 패턴에 따른 벼의 Lipid Transfer Protein Gene (LTP)의 발현 차이

  • Kim, Sun-Hee (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Song, Mira (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jang, Duk-Soo (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Si-Yong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin-Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Sang Hoon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Ha, Bo-Keun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Yong Dae (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Dong Sub (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 김선희 (한국원자력연구원 정읍방사선과학연구소) ;
  • 송미라 (한국원자력연구원 정읍방사선과학연구소) ;
  • 장덕수 (한국원자력연구원 정읍방사선과학연구소) ;
  • 강시용 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김진백 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김상훈 (한국원자력연구원 정읍방사선과학연구소) ;
  • 하보근 (한국원자력연구원 정읍방사선과학연구소) ;
  • 박용대 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김동섭 (한국원자력연구원 정읍방사선과학연구소)
  • Received : 2011.02.22
  • Accepted : 2011.02.28
  • Published : 2011.03.30

Abstract

In this study, we investigated to evaluate differential expression of genes encoding lipid transfer proteins (LTP) by acute and chronic gamma irradiation in rice. After acute and chronic gamma irradiation by 100 Gy and 400 Gy to rice plant, necrotic lesion was observed in the leaf blade and anthocyanin contents were increased. We isolated a total of 21 rice lipid transfer protein (LTP) genes in the TIGR database, and these genes were divided into four different groups on the basis of nucleotide sequences. The LTP genes also were classified as different four classes according to expression pattern using RT-PCR. Group A, B contained genes with increased expression and decreased expression in acute and chronic, respectively. Group C contained genes with contrasted expression pattern. Group D wasn't a regular pattern. But the specific affinity was not obtained between two grouping.

Keywords

Acknowledgement

Supported by : 교육과학기술부