• 한국어류학회지
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• 제21권4호
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• pp.287-290
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• 2009

줄종개(Cobitis tetralineata)와 왕종개(Iksookimia longicorpa)간 자연잡종으로 추정되는 개체를 유전적으로 동정하기 위하여 핵 recombination activating gene 1 (RAG1)과 미토콘드리아 cytochrome c oxidase I (COI) 유전자들의 염기서열을 분석하였다. RAG1 염기서열을 분석한 결과 850 bp 중에서 두 친어종들 간에 총 23개의 치환이 관찰되었고, 자연잡종 개체의 electropherogram에서는 이들 치환이 관찰된 모든 위치들에서 double peak들이 관찰되어, 멘델의 유전법칙을 따랐다. 그리고 모계를 통해 자손에게 유전되는 특징을 가지는 미토콘드리아 유전자들 중에서 COI 염기서열을 비교한 결과 잡종 개체는 줄종개와 염기서열이 100% 일치하여 그 모계는 줄종개임이 명확히 밝혀졌다.

• 한국가금학회지
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• 제36권1호
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• pp.85-88
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• 2009

조류에서 미토콘드리아내 유전자인 cytochrome c oxidase I(COI)는 종 구분을 위한 바이오마커로 많이 이용이 되고 있다. 본 연구는 이 유전자의 변이를 이용하여 닭의 품종 구분이 가능한지를 실험하기 위하여 실시하였다. 그 결과 한국 재래닭은 공시축으로 사용한 산란계인 White Leghorn과 육계인 Cornish가 가지고 있는 단일염기다형을 모두 가지고 있는 것으로 판명되어 품종구분을 위한 마커로 사용하기에는 적합하지 않은 것으로 확인되었다. 그러나 본 연구 결과를 통하여 한국재래계가 육계와 산란계의 특성을 모두 가지고 있다는 기존의 학설을 뒷받침하였으며, 품종 구분을 위하여 미토콘드리아와 genomic DNA 유래 단일염기다형 선발의 중요성을 확인할 수 있었다.

• 한국수산과학회지
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• 제48권5호
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• pp.731-738
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• 2015

Nematode infection in the epithelial tissue of cultured rockfish Sebastes schlegeli was first reported in 2012. Since then, nematode infections have caused serious economic losses in rockfish aquaculture on the west coast of Korea. Taxonomic and life cycle information for this parasite are currently unknown. In this study, 18S rRNA and cytochrome c oxidase subunit I (COI) genes were used for molecular identification and polymerase chain reaction (PCR) to detect the invisible stages of this parasite. Nucleotide sequences of the 18S rRNA of the rockfish nematode showed 98% identity with that of Philometra morii. Therefore, this rockfish nematode was classified to the Philometridae family. However, we could not identify it to genus level using 18S rRNA. Its COI nucleotide sequences shared 85% and 82% identities with those of Bursaphelenchus sinensis and Philometra overstreeti, respectively. In addition, two gene-specific primer sets were designed based on the 18S rRNA gene to detect the intermediate host and nematode larvae. These primers were specific to this rockfish nematode without cross-reacting to other pathogens. The detection limit of the PCR assay using these primers was 1,000 copies of nematoda plasmid DNA. Therefore, the PCR assay described here is suitable for the detection of nematode DNA within rockfish. In addition, this PCR assay could be used to detect nematode larvae and the intermediate host.

• 한국어류학회지
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• 제29권4호
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• pp.244-251
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• 2017

본 연구는 2016년에 한국 남해에서 채집된 둥글넙치과 자어를 분자방법으로 동정하였으며, 자어의 크기별 외부 형태를 상세히 기술하였다. 채집된 자어 15개체 (3.53~19.49 mm 체장)의 mitochondrial DNA (mtDNA) cytochrome c oxidase subunit I gene (COI) 434 bp의 염기서열을 비교한 결과, 모두 사량넙치로 확인되었다. 사량넙치의 자어는 2개의 길게 연장된 등지느러미 기조를 가지며, 등지느러미와 뒷지느러미 기저에 흑색소포가 일렬로 나 있는 반면 체측에는 흑색소포가 없었다. 성장하면서 체장에 대한 두장 및 두고의 비율이 증가 추세에서 감소 추세로 바뀌는 변곡점이 체장 9.93 mm~10.73mm에서 확인되었다. 사량넙치 자어는 형태적으로 가장 유사한 동백가자미(Psettina iijimae)와 달리 등지느러미와 뒷지느러미의 기저 근처에 흑색소포군을 가지지 않는 점에서 잘 구분된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권2호
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• pp.181-189
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• 2003

We describe here the complete nucleotide sequence and the exon-intron structure of the luciferase gene of the firefly, Pyrocoelia rufa. The luciferase gene of the P. rufa firefly consisted of six introns and seven exons coding for 548 amino acid residues. From the translational start site to the end of last exon, however, the genomic DNA length of the P. rufa luciferase gene from the Korean and Chinese samples spans 1,968 bp and 1983 bp, respectively, and 3 amino acid residues were different to each other. Additionally, we also analyzed mitochondrial cytochrome oxidase I(COI) gene of the Chinese P. rufa fireflies. Analysis of DNA sequences from the mitochondrial COI protein-coding gene revealed 4 mitochondrial DNA sequence-based haplotypes with a maximum divergence of 0.7％. With the 20 P. rufa haplotypes found in Korea, phylogenetic analyses using PAUP and PHYLIP subdivided the P. rufa into three clades, termed clades A and B for the Korean sample, and clade C for the Chinese sample.

• 한국식품위생안전성학회지
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• 제35권1호
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• pp.45-50
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• 2020

본 연구는 원주시 내 대형 초밥 뷔페에서 제공되는 초밥, 회 등 26개 수산물 가공품을 대상으로 DNA 바코드를 분석하여 원재료의 종을 동정하였다. DNA 바코드 증폭을 위하여 미토콘드리아의 16S ribosomal RNA 및 cytochrome c oxidase subunit I 유전자 부위를 증폭하는 프라이머 세트를 이용하여 증폭된 PCR 산물의 염기서열을 분석하였다. 확보한 염기서열은 BLAST Search를 이용하여 미국국립보건원 GenBank에 등록되어있는 생물 종의 염기서열과 비교하여 염기서열 유사도와 매칭 점수를 고려하여 최종 종을 동정하였다. 모니터링 결과 분석 제품의 58%는 제품명과 사용된 원재료가 일치하였지만, 27%에서는 불일치가 관찰되었다. 초메기초밥에는 가이양(Pangasianodon hypophthalmus)이 꽃돔회에는 붉평치(Lampris guttatus)가 사용되었으며, 날치알군함 및 청어알무침에는 열빙어(Mallotus villosus) 알이 사용되었음을 확인하였다. 타코와사비군함 및 오징어간장소스에 사용된 원재료는 주꾸미(Amphioctopus fangsiao) 및 남방주꾸미(Amphioctopus membranaceus)로 각각 동정되었다.

• Genomics & Informatics
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• 제10권3호
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• pp.206-211
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• 2012

DNA barcoding has been widely used in species identification and biodiversity research. A short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence serves as a DNA bio-barcode. We collected DNA barcodes, based on COI sequences from 156 species (529 sequences) of fish, insects, and shellfish. We present results on phylogenetic relationships to assess biodiversity the in the Korean peninsula. Average GC% contents of the 68 fish species (46.9%), the 59 shellfish species (38.0%), and the 29 insect species (33.2%) are reported. Using the Kimura 2 parameter in all possible pairwise comparisons, the average interspecific distances were compared with the average intraspecific distances in fish (3.22 vs. 0.41), insects (2.06 vs. 0.25), and shellfish (3.58 vs. 0.14). Our results confirm that distance-based DNA barcoding provides sufficient information to identify and delineate fish, insect, and shellfish species by means of all possible pairwise comparisons. These results also confirm that the development of an effective molecular barcode identification system is possible. All DNA barcode sequences collected from our study will be useful for the interpretation of species-level identification and community-level patterns in fish, insects, and shellfish in Korea, although at the species level, the rate of correct identification in a diversified environment might be low.

• Molecules and Cells
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• 제22권3호
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• pp.323-327
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• 2006

DNA barcoding, an inventory of DNA sequences from a standardized genomic region, provides a bio-barcode for identifying and discovering species. Several recent studies suggest that the sequence diversity in a 648 bp region of the mitochondrial gene for cytochrome c oxidase I (COI) might serve as a DNA barcode for identifying animal species such as North American birds, insects and fishes. The present study tested the effectiveness of a COI barcode in discriminating Korean bird species. We determined the 5' terminus of the COI barcode for 92 species of Korean birds and found that species identification was unambiguous; the genetic differences between closely related species were, on average, 25 times higher than the differences within species. We identified only one misidentified species out of 239 specimens in a genetic resource bank, so confirming the accuracy of species identification in the banking system. We also identified two potential composite species, calling for further investigation using more samples. The finding of large COI sequence differences between species confirms the effectiveness of COI barcodes for identifying Korean bird species. To bring greater reliability to the identification of species, increased intra- and interspecies sampling, as well as supplementation of the mitochondrial barcodes with nuclear ones, is needed.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제4권3호
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• pp.104-114
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• 2023

In this study, the distribution, bait preference, hosts, symbionts, habitat status, and cytochrome c oxidase subunit-I sequences of the ant Linepithema humile were analyzed. This species habitat comprised approximately 1.5 km along stone crevices, flower gardens, roadsides, and container yards in the vicinity of Busan station, Korea. Feeding activity of this species was confirmed in 9 species of 7 families of plants. Which was approximately 14% of total flora (63 species of 37 families) in the studied area. Particularly, it was observed that Dendranthema indicum and Camellia japonica were the most frequently visited. Further, we verified that they interacted with four species of aphids. We identified 22 species of ants (17 genera, 4 subfamilies) inhabiting the Busan station area, including L. humile, Tetramorium tsushimae, Nylanderia flavipes and the alien species Paratrechina longicornis. In areas where L. humile was the dominant species, the habitat of local native ants was reduced by approximately 30%. Bait preference was ranked in the following order: jelly, water with glucose (25%), and yogurt, with the highest preference for baits containing sugar and protein. The 630-bp cytochrome c oxidase subunit-I sequences of the local L. humile populations were 99% identical to those of L. humile in the National Center for Biotechnology Information database, and the Argentine ants that invaded Korea had a sequence identical to that of other invasive populations from China, Japan, and New Zealand. As L. humile rapidly increases due to domestic adaptation, continuous monitoring and control strategy for eradication are needed to protect domestic biodiversity.

• 한국어병학회지
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• 제36권2호
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• pp.403-408
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• 2023

본 연구를 통해 한국의 양식 가숭어에서 최초로 분리된 등각류는 Nerocila japonica와 가장 근연한 종으로 확인되었으나, 향후 형태학적 분류 등 명확한 종 동정이 추가로 필요할 것으로 판단되며, 약물의 직접 노출에 의한 구충 효과를 확인한 결과 trichlorfon이 가장 효과가 있는 것으로 확인되었다.