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Genomic Structure of the Luciferase Gene and Phylogenetic Analysis of the Firefly, Pyrocoelia rufa  

Jianhong Li (College of Natural Resources and Life Science, Dong-A University)
Park, Yong-Soo (College of Natural Resources and Life Science, Dong-A University)
Zhao Feng (Department of Plant Protection, College of Plant Science and Technology, Huazhong Agricultural University)
Kim, Iksoo (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology)
Lee, Sang-Mong (Department of Sericultural and Entomological Biology, Miryang national University)
Kim, Jong-Gill (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology)
Kim, Keun-Young (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology)
Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University)
Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.7, no.2, 2003 , pp. 181-189 More about this Journal
Abstract
We describe here the complete nucleotide sequence and the exon-intron structure of the luciferase gene of the firefly, Pyrocoelia rufa. The luciferase gene of the P. rufa firefly consisted of six introns and seven exons coding for 548 amino acid residues. From the translational start site to the end of last exon, however, the genomic DNA length of the P. rufa luciferase gene from the Korean and Chinese samples spans 1,968 bp and 1983 bp, respectively, and 3 amino acid residues were different to each other. Additionally, we also analyzed mitochondrial cytochrome oxidase I(COI) gene of the Chinese P. rufa fireflies. Analysis of DNA sequences from the mitochondrial COI protein-coding gene revealed 4 mitochondrial DNA sequence-based haplotypes with a maximum divergence of 0.7％. With the 20 P. rufa haplotypes found in Korea, phylogenetic analyses using PAUP and PHYLIP subdivided the P. rufa into three clades, termed clades A and B for the Korean sample, and clade C for the Chinese sample.
Keywords
Firefly; Pyrocoelia rufa; Luciferase gene; Mitochondrial DNA; Cytochrome oxidase I gene; Phylogenetic analysis;
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