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Identification of a Natural Hybrid between the Striped Spine Loach Cobitis tetralineata and the King Spine Loach Iksookimia longicorpa by Analyzing Mitochondrial COI and Nuclear RAG1 Sequences  

Lee, Il-Ro (Department of Marine Biotechnology, Soonchunhyang University)
Yang, Hyun (Biological Diversity Research Institute)
Kim, Jong-Hwan (Department of Marine Biotechnology, Soonchunhyang University)
Kim, Keun-Yong (Department of Marine Biotechnology, Soonchunhyang University)
Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
Publication Information
Korean Journal of Ichthyology / v.21, no.4, 2009 , pp. 287-290 More about this Journal
Abstract
A natural hybrid between the striped spine loach Cobitis tetralineata and the king spine loach Iksookimia longicorpa was genetically identified by sequence analyses of nuclear recombination activating gene 1 (RAG1) and mitochondrial cytochrome c oxidase I (COI) genes. Out of 850 base positions of RAG1, a total of 23 nucleotide substitutions were detected between the two parental species, whereas the electropherogram of the natural hybrid displayed double peaks at all of the 23 positions, which reflects their simple Mendelian inheritance pattern. Meanwhile, comparison of partial sequences of mitochondrial genes (COI in this study), which are well characterized by the maternal inheritance pattern, revealed that the maternal species of the hybrid was C. tetralineata because of their 100% sequence identity.
Keywords
Natural hybrid; Cobitis tetralineata; Iksookimia longicorpa; COI; RAG1;
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Times Cited By KSCI : 1  (Citation Analysis)
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