• Journal of Life Science
• /
• v.8 no.2
• /
• pp.126-130
• /
• 1998

A pair of designed primers (sequences from Gene Bank) amplified 16S fRNA gene of V. vulnificus within polymerase chain reaction (PCR) machine. This PCR product is about 1.3kb DNA fragment. Six enzymes (BamH I, Alu I, Sau3A I, Hind III, Sal I, Sma I) were used for restriction pattern analysis of amplified 16S rRNA gene of V. vulnificus ATCC 27562. Digested fragments are resolved by 3% agarose gel. BamH I did not show digested fragment so, there was no cutting site of BamH I in PCR product. Alu I produced three small fragments from 400 bp to 200 bp. Sau3A I produced three fragments larger than Alu I from 70 bp and 500 bp. One of fragments of Sal I was same with 500 bp of Hind III fragment and the other was 750 bp. Sma I showed two fragments of 800 bp and 470 bp. The profile of digested fragments of 16S rRNA of V.vulnificus ATCC 27562 will may be able to use standard profile for identification of V. vulnificus.

• Journal of the Korean Geosynthetics Society
• /
• v.16 no.4
• /
• pp.163-176
• /
• 2017

The Discrete Element Method (DEM) has been widely used in civil engineering as well as various industrial fields to simulate granular materials. In this study, DEM was adopted to predict the performance of the face plate-type earth pressure balance (EPB) shield TBM (Tunnel Boring Machine). An analysis of the TBM excavation performance was conducted according to two pre-defined excavation conditions with the different rotation speeds per minute (RPM) of the cutterhead. The TBM model which was used in this study has a 6.64 m of diameter and six spokes. Also, 37 precutters and 98 scrapers at an each spoke were modeled with a real-scale specification. From the analysis, compressive forces at the cutterhead face, shield and cutting tools, resistant torques at the cutterhead face, muck discharge rate and accumulated muck discharge by the screw auger were measured and compared.

• Archives of Pharmacal Research
• /
• v.28 no.5
• /
• pp.619-625
• /
• 2005

Beads loaded with the water-soluble drug, phenylpropanolamine HCl (PPA), were prepared using an extruder and double arm counter-rotating roller modified from a traditional pill machine. The mean diameter of the cylindrical rod-like extrudate from the ram extruder was 3 mm; that of the uncoated bead after cutting and spheronization by the modified double arm counter-rotating roller was 3.26~3.28 mm. Although the surface of the beads was moderately rough and irregular, some exhibited hump-shaped protrusions, the sphericity was acceptable (roundness 1.15) and adequate for the subsequent coating process. An increase in mean diameter of the coated beads and improvements in friability and sphericity were observed in proportion to the amount of coating material applied (ethylcellulose or Eudragit？？ RS 100). It was also found that the release rate of PPA from the coated beads could be controlled by the amount and type of coating materials applied or with the incorporation of Eudragit ？？ RS 100 into the core matrix. Further modifications to the double arm counter-rotating roller, including adjustment of the rotation speed and distance between the rollers, would yield smaller uncoated beads with improved roundness and surface roughness. In conclusion , the present method could be potentially applied to prepare controlled release drug delivery beads or pellet dosage forms.

• Journal of Technologic Dentistry
• /
• v.39 no.1
• /
• pp.35-42
• /
• 2017

Purpose: The purpose of this paper was to evaluate the occurrence of errors regarding adaptation by conducting a three-dimensional assessment comparing the bridge type dental restoration after the cutting process, which has multiple abutments, with a single type dental restoration. Methods: By using ten identical files obtained by scanning the master model, thirty designs were created consisting of ten maxillary right first premolars and ten maxillary right first molars with single crown abutments, along with ten bridge designs with the identical abutment. A 5-axis milling machine was used to produce the design file. The produced denture prostheses were scanned using a silicone replica for a STL file. An evaluation was conducted using 3D analysis software on the master model and each of the thirty data files. Results: The RMS value of the pre-molar (14) was $38.4{\pm}4{\mu}m$ for single and $54.7{\pm}6{\mu}m$ for bridge abutment; therefore, a statistically significant difference was observed for single and bridge designs although both shared the same abutment form (P<.05). Also, the RMS value of the molar (16) was $47.6{\pm}2{\mu}m$ and $56.6{\pm}5{\mu}m$ for the single and bridge designs, respectively, thereby presenting a statistically significant difference (P<.05). Conclusion: As a result, dental prosthesis fabricated using the single method presented better internal adaptation outcomes.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2016.05a
• /
• pp.731-733
• /
• 2016

The primary goal of the line tracer is to accurately and quickly detect the movement up to the target position given by the sensor juhaengseon. It has been used in applications in various fields such as the current unmanned transport vehicles, laser cutting machine, autonomous mobile robots and unmanned driving is possible, and is held annually at various universities in the competition field with the possibility of great progress, depending on the application. However, there arises a large difference in running performance, depending on the hardware design and control. In this paper, improving the characteristics of the tracer line and characters to design a PID controller is to apply the point on ways of improving the properties of the system.

• Journal of Computational Design and Engineering
• /
• v.1 no.1
• /
• pp.27-36
• /
• 2014

Similar to the essential components of many mechanical systems, the geometrical properties of the teeth of spiral bevel gears greatly influence the kinematic and dynamic behaviors of mechanical systems. Logarithmic spiral bevel gears show a unique advantage in transmission due to their constant spiral angle property. However, a mathematical model suitable for accurate digital modeling, differential geometrical characteristics, and related contact analysis methods for tooth surfaces have not been deeply investigated, since such gears are not convenient in traditional cutting manufacturing in the gear industry. Accurate mathematical modeling of the tooth surface geometry for logarithmic spiral bevel gears is developed in this study, based on the basic gearing kinematics and spherical involute geometry along with the tangent planes geometry; actually, the tooth surface is a parametric surface defined on a parallelogrammic domain. Equivalence proof of the tooth surface geometry is then given in order to greatly simplify the mathematical model. As major factors affecting the lubrication, surface fatigue, contact stress, wear, and manufacturability of gear teeth, the differential geometrical characteristics of the tooth surface are summarized using classical fundamental forms. By using the geometrical properties mentioned, manufacturability (and its limitation in logarithmic spiral bevel gears) is analyzed using precision forging and multiaxis freeform milling, rather than classical cradle-type machine tool based milling or hobbing. Geometry and manufacturability analysis results show that logarithmic spiral gears have many application advantages, but many urgent issues such as contact tooth analysis for precision plastic forming and multiaxis freeform milling also need to be solved in a further study.

• Journal of Convergence for Information Technology
• /
• v.7 no.4
• /
• pp.75-81
• /
• 2017

The design trend of information technology is quickly changed with the times. The design trend of information display is a bezel-less display, recently. The bezel-less display or edge-less display is a new trend of mobile phone display. In this study, the shading tape was manufactured for assembling process of touch panel display with the high screen-to-body ratio so-called bezel-less display. The shading tape was fabricated on PET film with the UV curable acrylic pressure sensitive adhesive(PSA) by roll-to-roll process. The UV curable PSA was synthesized with the eco-friendly toluene-less manufacturing method. The adhesive power of manufactured shading tape was investigated by motorized tensile testing machine. The thixotropic, maintaining property of cutting shape, was characterized by field emission scanning electron microscope. As results, the shading tape exhibits high adhesive power and good thixotropic performance suitable for assembly process of touch panel display. The functional shading tape will be expected to improve productivity of assembly process of touch panel display.

• Journal of the Korean Society of Safety
• /
• v.20 no.4 s.72
• /
• pp.14-19
• /
• 2005

One area in which composites have been used rather extensively is for fabricating pressure vessel. These structures can be readily manufactured by filament winding, which is, as far as composite fabrication techniques are concerned, a relatively inexpensive method for producing composite structures. Unfortunately, the higher strength material and fabrication costs are not the only disadvantages of fiber-reinforced polymer composites when they are compared to metals. Additionally, these materials tend to exhibit brittle behavior. This is of particular concern when they are subjected to a low-velocity impact during routine handling a significant amount of structural damage can be introduced into the composites. The goals of this paper are to understand the impact damage behavior and identify the effect of surface coating materials on impact resistance in filament wound composite pressure vessels. For these, a series of low velocity impact tests was performed on specimens cutting from the full scale pressure vessel by the instrumented impact testing machine. The specimens are classified into two types with and without surface protective material. The visualization for impact damage is made by metallurgical microscope. Based on the impact force history and damage, the resistance parameters were employed and its validity in identifying the damage resistance of pressure vessel was reviewed. As the results, the impact resistance of the filament wound composites and its dependency on the protective material were evaluated quantitatively.

• Journal of Fashion Business
• /
• v.17 no.1
• /
• pp.131-144
• /
• 2013

The purpose of this study was to analyse the structure and contents of clothing construction field in the middle-school textbook of technology and home economics with curriculum revised in 2007. 10 kinds of textbooks certified by Ministry of Education, Science and Technology in 2010 were selected and home economics field in them was the subject of this study with the external characteristics such as textbook system, instruction area and contents included. This study was content analysis-oriented and descriptive with data calculated by frequency and percentage. The findings are as follows. In 'Reality of Home-Life', food life area showed highest as 34.4% followed by clothing life(28.7%) and dwelling life(28.7%). The portion of present teachers in writing staffs was highest while there was no textbook where professors of each field were included in writing staffs. The contents of costume society, clothes material, clothes management from section 'Choice and Management of Clothes' in the chapter 'Family Life' and clothing construction from the section 'Making and Modifying Clothes' of the chapter 'Reality of Home-Life' were analysed in this study. Clothing construction-related fields were suggested separately by method of measurement, construction theory, drafting theory, drafting an original form, fabric trimming, utilization of the original form, length measure, cloth cutting, hand stitching, how to use a sewing machine, basting and correction, needlework process, completion, and evaluation. The contents of the unit 'Making Shorts' were about clothing construction theory, human body measurement, drafting an original form, process of making shorts and activity/research/experience. The contents of the unit 'Wearing Clothes after Fixing' in clothing construction-related field were about how to fix and recycling.

• Restorative Dentistry and Endodontics
• /
• v.39 no.4
• /
• pp.282-287
• /
• 2014

Objectives: The aim of the present study was to investigate the bond strength of RelyX Unicem (3M) to root canal dentin when used as an endodontic sealer. Materials and Methods: Samples of 24 single-rooted teeth were prepared with Gates Glidden drills and K3 files. After that, the roots were randomly assigned to three experimental groups (n = 8) according to the filling material, (1) AH Plus (Dentsply De Trey GmbH)/Gutta-Percha cone; (2) Epiphany SE (Pentron)/Resilon cone; (3) RelyX Unicem/Gutta-Percha cone. All roots were filled using a single cone technique associated to vertical condensation. After the filling procedures, each tooth was prepared for a push-out bond strenght test by cutting 1 mm-thick root slices. Loading was performed on a universal testing machine at a speed of 0.5 mm/min. One-way analysis of variance and Tukey test for multiple comparisons were used to compare the results among the experimental groups. Results: Epiphany SE/Resilon showed significantly lower push-out bond strength than both AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p < 0.05). There was no significant difference in bond strength between AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p > 0.05). Conclusions: Under the present in vitro conditions, bond strength to root dentin promoted by RelyX Unicem was similar to AH Plus. Epiphany SE/Resilon resulted in lower bond strength values when compared to both materials.