• 한국식생활문화학회지
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• 제23권4호
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• pp.469-478
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• 2008

In this study, degree of rancidity and trans fatty acid formation were examined in fat and oils, including soybean oil (SB), canola oil (CA), corn germ oil (CO), olive oil (OL), palm oil (PO), and beef tallow (BT), during heating for 10-130 minutes at 160-200$^{\circ}C$. In order to determine the rancidity of the fat and oils, acid values (AV), iodine values (IV), viscosity, and color were measured. Changes in the amounts of fatty acids and the formation of trans fatty acids were measured using GC and HPLC. For all groups, AV increased, IV decreased, and coefficients of viscosity and color increased as the heating temperature and heating time increased, indicating there were positive correlations between the heating temperature and time and AV. In addition, all groups had similar amounts of trans fatty acids, with the exception of the beef tallow; however, its level only slightly increased with heating. The olive oil had the lowest trans fatty acid content and the lowest amount created by heating. The order of trans fatty acid amounts generated while heating was BT>PO>CO>CA>SB>OL. According to the study results, the deep frying temperature during cooking should be 160-180$^{\circ}C$ in order to reduce AV and the amount of trans fatty acids that are formed. In addition, it is better to remove beef tallow during cooking and avoid heating at high temperatures since it results in high levels of trans fatty acids. The correlation between the amount of trans fatty acids and AV was positive, while the correlation between the amount of trans fatty acids and IV was negative, indicating that AV and trans fatty acid levels increase while IV decreases as the deep frying temperature and time increase. From the results, it was found that reducing the deep frying temperature and time can lessen increases in AV and trans fatty acids, and decrease IV. Accordingly, to reduce AV and trans fatty acid formation, the ideal deep frying conditions would be to use olive oil or soybean oil rather than beef tallow or palm oil at a temperature of 160-180$^{\circ}C$.

• 문화기술의 융합
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• 제10권2호
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• pp.419-427
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• 2024

지하철 공사장 인근에서 천공작업 중 부주의로 지하에 매설된 도시가스 배관에 구멍을 발생시켜 새어 나온 가스가 우수관 및 하수관을 통하여 지하 공사장 내부로 유입 정전기 등의 점화원과 접촉 폭발할 경우 피해 영향범위 및 위협구역을 ALOHA 프로그램에 적용 산정하였다. 도시가스 배관의 길이,직경 및 압력 등 다양한 조건을 입력하여 위협구역 산정결과 증기구름 가연성지역의 Red Zone는 1.2~1.4km, 폭발지역의 Yellow Zone는 0.8~1.0km 및 제트화재의 Red Zone는 45~61m로 나타났다. 이 연구에서는 증기구름의 가연성 지역에서 농도와 조건이 적절히 조합된 상태이면 가연성을 증가시키고, 폭발지역 내부에서는 유리창이 깨질 수 있는 압력인 1.0psi로 폭발이 일어날 수 있으며, 제트화재인 경우에는 높은 온도와 열복사가 발생 주위 건물 밀집지역으로 화재가 빠르게 확산할 뿐만 아니라 열복사 영향으로 많은 인명피해가 발생할 수 있음을 영향범위 및 위협구역 범위로 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제21권1호
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• pp.71-80
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• 2011

Four diazinon-degrading bacteria were isolated from agricultural soil by using an enrichment technique. The biochemical analysis and molecular method including RFLP indicated that these isolates were identical, and one strain designated DI101 was selected for further study. Phylogenetic analysis based on 16S rDNA sequencing indicated that the strain DI101 clearly belongs to the Serratia marcescens group. The ability of the strain to utilize diazinon as a source of carbon and phosphorus was investigated under different culture conditions. The DI101 strain was able to completely degrade 50 mg/l diazinon in MSM within 11 days with a degradation rate of 0.226 $day^{-1}$. The inoculation of sterilized soil treated with 100 mg/kg of diazinon with $10^6$ CFU/g DI101 resulted in a faster degradation rate than was recorded in non-sterilized soil. The diazinon degradation rate by DI101 was efficient at temperatures from 25 to $30^{\circ}C$ and at pHs from 7.0 to 8.0. The degradation rate of diazinon was not affected by the absence of a phosphorus supplement, and addition of other carbon sources (glucose or succinate) resulted in the slowing down of the degradation rate. The maximum degradation rate ($V_{max}$) of diazinon was 0.292 $day^{-1}$ and its saturation constant ($K_s$) was 11 mg/l, as determined by a Michaelis-Menten curve. The strain was able to degrade diethylthiophosphate-containing organophosphates such as chlorpyrifos, coumaphos, parathion, and isazofos when provided as a source of carbon and phosphorus, but not ethoprophos, cadusafos, and fenamiphos. These results propose useful information for the potential application of the DI101 strain in bioremediation of pesticide-contaminated environments.

• Ocean and Polar Research
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• 제42권2호
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• pp.133-139
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• 2020

The aim of this study is to examine the physiological characteristics of an agarophyte Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N. Norris et Fredericq in the early life stage of tetrasporophytes (2n) and gametophytes (n) to select appropriate seedlings for mariculture. Growth experiments were carried out at the combinations of four temperatures (20, 25, 30, and 35℃) and three light intensity levels (20, 60, and 100 µmol photons m^-2 s^-1) in the two ontogenetic stages: discoid holdfasts and erect sporelings. Holdfast areas and sporeling lengths of tetrasporophytes and gametophytes were estimated after 14 days in culture. Relative growth rates (RGRs) for holdfast areas were 7.08-28.38% day^-1 for tetrasporophytes and 11.58-23.67% day^-1 for gametophytes. At 35℃, holdfasts of tetrasporophytes survived with RGRs of 7.08-23.28% day^-1 but those of gametophytes died. Maximal holdfast growth of tetrasporophytes occurred at 30℃ and 100 µmol photons m^-2 s^-1, which were different from gametophytes (25℃ and 100 µmol photons m^-2 s^-1). RGRs of tetrasporophytic sporelings were 2.93-11.11% day-1 and were between 0.78-10.82% day-1 for gametophytes. Maximal growth of A. vermiculophyllum sporelings occurred at 25℃ and 60 µmol photons m^-2 s^-1 for tetrasporophytes, and at 20℃ and 100 µmol photons m^-2 s^-1 for gametophytes. In conclusion, the present results indicate that carpospores could be used as resources of spore-seedling methods having genetic diversity for mass field cultivation because tetrasporophytes showed higher-temperature tolerance and faster-growing ability than gametophytes of A. vermiculophyllum in the discoid holdfast and sporeling stages.

• 한국지역사회생활과학회지
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• 제19권3호
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• pp.365-371
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• 2008

This study was conducted to determine the potent physiological activity of traditional wild tea ("Don tea"; coin-shaped tea) as affected by different harvesting times, aging periods and extracting temperatures, No difference in anti-oxidative activities in the harvesting time and extracting temperature of tea leaves was observed. However, short aging periods of Don tea showed high ABTS {2-2-azonobis (3-ethylbenzothiazolin-6-sulphonic acid)} activity, ranging from 71.52 to 79.96%. DPPH (${\alpha},\;{\alpha}-diphenyl-{\beta}-picryl-hydrazyl$) radical scavenging activity of Don tea was 71.10 to 91.40%. Especially, longer aging period and an extracting temperature of $100^{\circ}C$ showed higher DPPH radical scavenging activity. With longer aging periods and an extraction at $90^{\circ}C$, nitrite radical scavenging activity of Don tea ranged from 74.04 to 94.92%. On the other hand, angiotensin 1-converting enzyme (ACE) inhibition activity of Don tea was 59.77-81.97%. It showed higher activity when harvested in June and August, aged for longer periods, and extracted at $100^{\circ}C$. These results suggested Korean traditional Don tea exhibited the highest physiological activity when aged over 8 months.

• 한국버섯학회지
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• 제16권2호
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• pp.79-85
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• 2018

ICT 기반 스마트 표고재배시설을 활용하여 표고재배의 과정을 후숙관리, 발이관리, 생육관리 및 휴양관리 4과정으로 나누어 6월부터 10월까지 시험을 진행하였다. 여름철 재배사 외부 $20{\sim}35^{\circ}C$의 고온환경에서도 재배사 내부의 환경은 설정한 방향으로 제어되었다. 여름철 대표적인 재배용 품종 '산조701호'와 신품종 '산조717호'를 이용한 재배시험 결과 '산조701호'의 경우 353.7 g/봉, '산조717호'는 270.4 g/봉으로 '산조701호'가 높은 버섯생산성을 나타내었다. ICT 기반으로한 스마트 표고재배시설을 활용한 표고재배는 재배과정별 환경조건 설정으로 재배가 어려운 고온의 환경에서도 편리하게 재배환경 관리가 가능하였으며, 재배품종에 적합한 환경제어 범위를 연구한다면 버섯생산 안정화 및 생산성 향상에 크게 기여할 것으로 판단되었다.

• 한국어병학회지
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• 제20권3호
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• pp.281-289
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• 2007

어병세균인 Edwardsiella tarda, Vibrio ichthyoenteri, Streptococcus iniae의 강도다리에 대한 병원성, 염분농도가 어병세균의 성장 및 강도다리의 lysozyme 활성과 사육수온, 밀도가 강도다리에 미치는 영향을 조사하였다.연구 결과, E. tarda에 대해서는 병원성이 높았으나, V. ichthyoenteri와 S. iniae에 대해서는 항병력이 높았다.염분농도에 따른 균의 증식속도 조사 결과, E. tarda와 S. iniae는 저염분에서 증식속도가 빨랐으나, V. ichthyoenteri는 저염분에서 낮았으나, 유의적인 차이는 보이지 않았다. 저염분 사육에서 강도다리의 lysozyme 활성은 일반해수구에 비하여 다소 낮게 나타났으나 유의적인 차이는 보이지 않았다.사육수온, 밀도가 강도다리에 미치는 영향을 조사한 결과, 수온은 26℃ 이하, 밀도는 수조저면적의 100% 이하가 강도다리의 생리학적 성상 및 lysozyme 활성이 높게 나타났다.

• 식물조직배양학회지
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• 제27권4호
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• pp.339-343
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• 2000

When plants are exposed to subfreezing temperatures ice crystals are forming within extracelluar space in leaves. The growth of ice crystal is closely related to the degree of freezing injury. It was shown that an antifreeze protein binds to an ice nucleator through hydrogen bonds to prevent growth of ice crystal and also reduces freezing damage. The antifreeze proteins in plants are similar to PR proteins but only the PR proteins induced upon cold acclimation were shown to have dual functions in antifreezing as well as antifungal activities. Three of the genes encoded for CLP, GLP, and TLP were isolated from barley and Kentucky bluegrass based on amino acid sequence revealed after purification and low temperature-inducibility as shown in analysis of the protein. The deduced amino acid of the genes cloned showed a signal for secretion into extracellular space where the antifreezing activity sup-posed to work. The western analysis using the antisera raised against the antifreeze proteins showed a positive correlation between the amount of the protein and the level of freeze tolerance among different cultivars of barely. Besides it was revealed that TLP is responsible for a freeze tolerance induced by a treatment of trinexapac ethyl in Kentucky bluegrass. Analysis of an overwintering wild rice, Oryza rufipogon also showed that an acquisition of freeze tolerance relied on accumulation of the protein similar to CLP. The more direct evidence for the role of CLP in freeze tolerance was made with the analysis of the transgenic tobacco showing extracellular accumulation of CLP and enhanced freeze tolerance measured by amount of ion leakage and rate of photosynthetic electron transport upon freezing. These antifreeze proteins genes will be good candidates for transformation into crops such as lettuce and strawberry to develop into the new crops capable of freeze-storage and such as rose and grape to enhance a freeze tolerance for a safe survival during winter.

• 한국균학회지
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• 제27권5호
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• pp.319-321
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• 1999

느타리버섯 종균을 배양 후 저장온도와 저장기간에 따른 잡균오염을, 배양적 특성 및 생산성을 조사하였다. 종균의 잡균오염율은 저온저장은 90일까지 0%였으며 상온저장은 60일에 6.3%였으며 120일에는 86.5%로 증가하였다. 저장 종균을 병재배용 배지에 접종하여 병재배 하였던 바, 균사 배양일수는 상온저장 종균은 $21{\sim}26$일로 저장기간이 길수록 길었으며, 저온 저장종균은 $21{\sim}22$일로 별 차이가 없었다. 저장 종균의 초발이 소요일수는 저장 기간이 길수록 길었으며, 저온저장 종균이 상온 저장 종균보다 $1{\sim}2$일 늦었다. 저장 종균 접종 후 수확까지의 재배기간은 상온저장종균이 $34{\sim}376$일, 저온저장종균이 $32{\sim}33$일로 저장기간이 길수록 증가하였다. 자실체 생중은 저장기간이 길수록 감소하였으나 상온저장 종균은 120일 처리에서 높았다. 배양율과 잡균오염율은 상온, 저온저장 모두 저장기간이 길수록 증가하였으며, 이를 감안한 버섯 생산성은 저온저장 종균이 상온저장 종균보다 많았으며 저장기간이 길수록 감소하였다.

• Childhood Kidney Diseases
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• 제20권2호
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• pp.57-62
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• 2016

Purpose: We used technetium-99m dimercaptosuccinic acid (DMSA) scintigraphy to identify factors predictive of renal cortical defects in infants <3 months of age with urinary tract infections (UTIs). Methods: We retrospectively reviewed data on infants <3 months of age with culture-proven UTIs treated at a single center from March 2010 to February 2016. Blood samples were obtained for laboratory evaluation prior to commencement of antibiotic therapy. The therapeutic delay time (TDT) and therapeutic response time (TRT) were recorded. All patients were divided into two groups depending on features of their DMSA scans. We compared the demographic, clinical, and laboratory characteristics of the two groups. Results: A total of 119 infants (94 males and 25 females; mean age, $56.9{\pm}21.3days$) were included. Cortical defects were evident in the DMSA scans of 47 cases (39.5%). In infants with such defects, the peak temperatures ($38.9{\pm}0.57^{\circ}C$ vs. $38.4{\pm}0.81^{\circ}C$, P=0.001), the absolute neutrophil counts ($8,920{\pm}4,460/mm$ vs. $7,290{\pm}4,090/mm$, P=0.043), and the C-reactive protein (CRP) levels ($6.49{\pm}4.33mg/dL$ vs. $3.21{\pm}2.81mg/dL$, P=0.001) were significantly higher than those in infants without cortical defects. The TDT was also longer in those with cortical defects (P=0.037). Conclusion: We found that a TDT ${\geq}8.5hr$ (odds ratio [OR] 5.81), a peak temperature ${\geq}38.3^{\circ}C$ (OR 6.19), and a CRP level ${\geq}4.96mg/dL$ (OR 7.26) predicted abnormal DMSA scan results in infants <3 months of age with UTIs.