• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.3
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• pp.239-248
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• 1997

This study was conducted to investigate the effects of antagonistic microorganisms, Bacillus spp., on growth of alfalfa(Medicag0 sativa L.) in repeated cultivation soil(RCS) and unrepeated cultivation soil(URCS). Alfalfa was established by seeding into pots 12 cm in diameter and 9 cm in depth containing 1 : 1 mixture of soil and vermiculite with antagonistic bacteria and pathogenic fungi. The growth experiment of alfalfa was conducted in pots in a vinyl house. The bacteria used in this study were Bacillus subtilis and hsants. B. subtilis was isolated and identified 60m forage rhizosphere soil and hsants isolated through cell fusion fiom B. subtilis 101 and B. thuringiensis. B. subtilis was named B. subtilis 101 and hsants named F -3 and F -8. From dark culture experimenf alfalfa was longer lived in treated soil with antagonistic bacteria than that in non-treated soil, and longer lived in URCS than that in RCS. However, alfalfa was shorter lived in RCS and URCS than that in autoclaved RCS. The number of leaves of alfalfa were positively affected by the inoculation of the antagonistic bacteria in both RCS and URCS. Dry weight of shoot and root was increased by the inoculation of the antagonistic bacteria(P< 0.05). However, the growth of alfalfa was decreased by the inoculation of the pathogenic hngi both RCS apd URCS.

• Korean Journal of Veterinary Research
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• v.42 no.1
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• pp.55-64
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• 2002

The envelope glycoprotein of HIV-1 forms an oligomeric complex resulting in playing a role to induce neutralizing antibody and cell-mediate immune responses. The oligomer exists as a trimer of gp120-gp41 heterodimer which mediates HIV-1 attachment and fusion. We made a cDNA clone of gp140 consisting of gp120 and ectodomain of gp41 from the primary African isolate. To express the oligomeric gp140 in mammalian cells, we adopted the Semliki Forest virus (SFV) based expression system. The oligomeric gp140 in the secretory form was expressed and purified from the cell culture supernatant and characterized. The antibody inducing activity of the purified gp140 was also examined in mice inoculation.

• Journal of Microbiology
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• v.38 no.4
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• pp.218-223
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• 2000

Using two drugs, tunicamycin and brefeldin A, which affect protein processing, we investigated the intracellular processing mechanism of secreted aspartyl proteinase 1 (SAPl) of Candide albicans. Three intracellular forms of SAPI were detected by immunoblotting using menoclonal antibody (MAb) CAPl. Their molecular weights were approximately 40, 41 and 45 kDa, respectively. The 41 kDa protein is a glycoprotein and may be the same as the extracellular form judging by its molecular mass. The 40 kDa protein was the unglycosylated form and its molecular mass coincided with deglycosylated SAPl and the 45 kDa protein was also the unglycosylated form. Neither the 40 and 45 kDa proteins were detected in the culture supernatant of C. albicans. These suggested that the 40 and 45 kDa proteins might be intracellular precursor forms of SAPI. These results show that SAPI is translated as a 45 kDa precusor form in the endoplasmic reticulum and the 45 kDa precursor farm undergoes proteolytic cleavage after translocation into the Golgi apparatus, generating the 40 kDa precursor form. This 40 kDa precursor is converted into a 41 kDa mature form through glycosylation in the Golgi apparatus. The mature form of the 41 kDa protein is sorted into secretary vesicles and finally released into the extracellular space through membrane fusion. When the glycan region of SAPl was digested with N-glycosidase F, both stability and activity of the enzyme decreased. These results indicate that the glycan attached to the enzyme may, at least in parti be related to enzyme stability and activity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.9
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• pp.3815-3826
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• 2011

TThis study was undertaken to make trial costumes of ball jointed dolls for promoting the beauty of Korean cultural product with historical research of upper class ladie's costume from late Goryeo to early Joseon, the very unknown period in the Korean costume history. The ladies' costume from late Goryeo to early Joseon are characterized by their full silhouette, fusion with clothings of Yuan and Ming, revival of early Goryeo styles, well-developed weaving skill of ramie, a showy costume from Buddhist civilization. After investigation of researcher with literature, painting, and relics in 13-14C, 8 clothing items were outsourced totally. Items were formed as a set concept, but consumer can choose each costume as an single item and can make varied mix also. Dart, small ties, and thick fabrics were used in doll costumes partly because ball jointed doll's body has different characteristics from that of human beings. Developing research of manufacturing company's system for ready-made product remains to be considered in the future.

• Journal of the Korean Institute of Intelligent Systems
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• v.15 no.5
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• pp.573-580
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• 2005

In this paper, we propose an emotion recognition using static and dynamic facial images to effectively design human interface. The proposed method is constructed by HMM(Hidden Markov Model), PCA(Principal Component) and wavelet transform. Facial database consists of six basic human emotions including happiness, sadness, anger, surprise, fear and dislike which have been known as common emotions regardless of nation and culture. Emotion recognition in the static images is performed by using the discrete wavelet. Here, the feature vectors are extracted by using PCA. Emotion recognition in the dynamic images is performed by using the wavelet transform and PCA. And then, those are modeled by the HMM. Finally, we obtained better performance result from merging the recognition results for the static images and dynamic images.

• Cross-Cultural Studies
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• v.29
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• pp.55-84
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• 2012

This paper is to study the Tang Dynasty monk poet Ling-yi's poem world and his significance on history of literature. In Chinese literature history, "the monk poets" is a very unique literature creation group. The means of a word "the monk poet", from the literal can see " he is the monk that Can write poems", which is dedicated to professionally writing poetry monk. Buddhism spread to China, from the Wei and Jin Dynasty beginning has been writing poetry of the monks, but the real meaning of "the monk poet" (i.e., professionally poetry monk) appeared to the Mid-Tang Dynasty period. The monk Ling-yi is the pioneer of the monk poets group and Buddist monk creative poem in Mid-tang Dynasty period. Although the Lingyi life is very short, only 35 had died, and his poetry has not been too much, only 44 songs, but he in this life of 35 years and 44 poems, for the development of classical Chinese poetry left noticeable imprinting. He is not just as monk's high practice and by advocating for great Buddhist scholar, also through the daily meditation in poetry creation practice were obtained at that time of many men of literature and writing respected. This paper from the poem monk Ling-yi double identity - the first is a Buddhist monk, the second is addicted to poetry poet to proceed, step by step, in-depth study as the poem monk Ling-yi's poetry creation characteristic and the creative mentality characteristics. This thesis also explores the poem monk by the creation of poetry pursuit, exploration, finally realized "poem" and "Zen" together as one "Zen poetry" creation mechanism.

• Journal of Convergence for Information Technology
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• v.8 no.6
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• pp.101-106
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• 2018

We examined refractive error, the corrected visual acuity, phoria and fusional reserve of 114 healthy myopes and hyperopia who had no strabismus, no ocular and physical diseases. We evaluated the occurrence of asthenopia according to phoria and fusional reserve. 41 out of 114 subjects which exophoria was 69% and esophoria was 5.5%, orthophoria was 26% made a complaint against asthenopia. The subjects who had exophoria of $0-6{\Delta}$ in the range of normal state was 61.4%, while the subjects who had exophoria of $7{\Delta}$ or more in the range of abnormal state was 38.6%. Reducing fusional reserve was associated with increasing phoria. The fusional reserve twice or more than phoria were 27.2%, and twice or under were 72.8%. Futhermore, AC/A ratio for heterophoria was found from 1.0 to 12.6 and its relationship to asthenopia of phoria could not be determined.

• Journal of Digital Convergence
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• v.20 no.3
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• pp.399-406
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• 2022

The purpose of this study is to increase the development possibility of K-pop music by analyzing K-pop music and analyzing the use of world music in it. As a research method, we will first look at the world music elements Puerto Rico's reggaeton, Jamaica's toasting, and the melody of Indian music. After that, we analyze two songs, Hyo-yeon's and Hyun-A & Dawn's , with rhythm and melody elements to find out the utilization of World Music. As a result of the study, it was found that it rhythmically showed the type of Dembow rhythm derived from Puerto Rico's reggaeton, and melody followed the form of Jamaican toasting and the melody of Arabic music. This study is meaningful in presenting the possibility of development and various directions of K-pop in the future by analyzing the use of world music used in K-pop music. Through the convergence of various world music and K-pop, I hope that K-pop music will become a culture that is supported by a wider public.

• Journal of Fashion Business
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• v.28 no.2
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• pp.50-63
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• 2024

Chinese traditional wedding dress is a unique cultural symbol. It had a deep historical background as it went through different productions in each dynasty. However, with the introduction of Western ideas, an increasing number of people are wearing Western-style wedding dresses when they are getting married. Recently, traditional Chinese culture has been increasingly valued. Demand for traditional Chinese wedding dresses among modern Chinese people is increasing. Following this trend, Chinese designer Guo Pei(1967~) is actively innovating elements of traditional Chinese wedding dresses. This study aimed to analyze changes in traditional wedding dress styles by dynasty in China from 1046 B.C. to 1911 A.D. based on museum data and previous research. Changes in traditional wedding dress styles by Chinese dynasty were analyzed and organized. The meaning of wedding dress patterns was analyzed by type using traditional Chinese patterns. This study focused on Guo Pei's 2012 "Chinese bride" series and "Legend of the dragon" series. Starting from the development and characteristics of traditional Chinese wedding dresses, this study analyzed fashion characteristics of Guo Pei's wedding dress series. Goals of this study were to preserve the essence of traditional Chinese wedding dresses; and to determine how to apply the fusion of traditional Chinese design elements into fashion and modern design to wedding dresses.

• The korean journal of orthodontics
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• v.25 no.6 s.53
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• pp.715-722
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• 1995

Orthodontic tooth movement in response to orthodontic force results from actions of osteoclasts and osteeoblasts in the cell level. Convincing evidence has now been provided to support the view that osteoclasts are derived from mononuclear cells that originate in the bone marrow or other hematopoietic organs and they migrate to the bones via vascular routes. Nitric oxide(NO), which accounts for the biological properties of endothelium-derived relaxing factor(EDRF), is the endogenous stimulator of soluble guanylate cylase. The discovery of the formation of nitric oxide(NO) from L-arginine in mammalian tissues and its biological roles has, in the last 7 years, thrown new light onto many areas of research. Data from experiments in vitro showed that N-metyl-L-arginine(L-NMA) and L-nitro-L- arginine(L-NAME) are competitive inhibitors of nitric oxide synthase. This study suggest that the multinucleated cells in our culture have characteristics of osteoclasts and that the potential bone cell activity of nitric oxide in vitro may be mediated in part by stimulation of marrow mononuclear cells to form osteoclast-like cells. Bone marrow cells were obtaineed from tibia of 19-days old chick embryo. After sacrifice, tibia was quickly dissected and the bone were then split to expose the medullary bone. The cells were attached for 4 hours and the nonadherent cells were collected. Marrow cells weere cultured in 96-well plate in medium 199. To examine the number of TRAP-positive multinucleated cells(MNCs), $10^{-8}\;M\;Vit=D_3$ and various concentration of L-NMA and L-NAME weere added at the beginning of cultures and with each medium change. After 7 days of culture. tartrate-resistant acid phosphatase(TRAP) staining was performed for microscopic evaluation. Cells haying more than three nuclei per cell were counted as MNCs. The obsrved results were as follows;1. 1,25-dihydroxyvitamine $D_3$ stimulated the osteoclast-like multinucleated cells in cultures of chick embryo bone marrow. 2. Nitric oxide synthase inhibitors(NOSI ; N-NMA, N-NAME) stimulated the osteoclast-like cells in cultures of chick embry bone marrow. 3. 1,25-dihydroxyvitamine$D_3$ and nitric oxide synthase inhibitors did not appear to have additive effect on the generation of TRAP-positive MNCs. These results suggest that nitric oxide synthase inhibitors may stimulate the osteoclast-like multinucleated cell formation and fusion in cultures of chick bone marrow.