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Expression and Characterization of Human Immunodeficiency Virus-1 Oligomerized gp140 Protein in Mammalian Cells  

Kim, Eun-Ok (College of Veterinary Medicine, Chungnam National University)
Kim, Eun (College of Veterinary Medicine, Chungnam National University)
Kim, Hyun-Soo (College of Veterinary Medicine, Chungnam National University)
Shin, Kwang-Soon (College of Veterinary Medicine, Chungnam National University)
Kim, Chul-Joong (College of Veterinary Medicine, Chungnam National University)
Publication Information
Korean Journal of Veterinary Research / v.42, no.1, 2002 , pp. 55-64 More about this Journal
Abstract
The envelope glycoprotein of HIV-1 forms an oligomeric complex resulting in playing a role to induce neutralizing antibody and cell-mediate immune responses. The oligomer exists as a trimer of gp120-gp41 heterodimer which mediates HIV-1 attachment and fusion. We made a cDNA clone of gp140 consisting of gp120 and ectodomain of gp41 from the primary African isolate. To express the oligomeric gp140 in mammalian cells, we adopted the Semliki Forest virus (SFV) based expression system. The oligomeric gp140 in the secretory form was expressed and purified from the cell culture supernatant and characterized. The antibody inducing activity of the purified gp140 was also examined in mice inoculation.
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1 XinZhen Yang, Michael Farzan, Richard Wyatt, and Joseph Sodroski. 2000. Characterization of stable trimers containing complete ectodomains of Humanimmunodeficiency virus type 1 envelope glycoproteins. 2000. Journal of Virology. June. 5716-5725
2 Letvin, N. L. 1998. Progress in the development of an HIV-1 vaccine. Science 280:1875-1880
3 Peter Liljestrom and Henrik Garoff. 1991. A new generation of animal cell expression vectors based on the Semliki Forest Virus replicon. Nature Biotechnology. 9:1356-1361
4 Heilman, C. A., and D. Baltimore. 1998. HIV vaccineswhere are we going? Nature Medicine, 4:532-534
5 Christopher C. Broder, Patricia L. Earl, Deborah Long, Stephen T. Abedon, Bernard Moss, and Robert W. Doms. Antigenic implications of human immunodeficiency virus type 1 glycoprotein quarternary structure: oligomerspecific and -sensitive monoclonal antibodies. 1994. Proc. Natl. Acad. Sci. USA 91:11699-11703
6 N.L. Paul, M. Marsh, J.A. Mckeating, T.F. Schulz, P. Liljestrom, H. Garoff, and R.A. Weiss. 1993. Expression of HIV-1 envelope glycoproteins by Semliki ForestVirus vectors. AIDS RESEARCH AND HUMAN RETROVIRUSES. 9:963-970
7 Abraham Pinter, William J. Honnen, Shermaine A. Tilley, Constantin Bona, Habib Zaghouani, Miroslaw K. Gorny, and Susan Zolla-Pazner. 1989. Oligomericstructure of gp41, the transmembrane protein of Human immunodeficiency virus type 1. Journal of Virology. June. 2674-2679
8 Patricia L. Earl, Robert W. Doms, and Bernard Moss. 1990. Oligomeric structure of the human immunodeficiency virus type 1 envelope glycoprotein. Proc. Natl. Acad. Sci. USA 87:648-652
9 Hung F, Ross F. Conner, Luis P. Villarreal, AIDS in brief. 1999. The biology of AIDS(4th Edition):2-5
10 Trkola, A., T. Dragic, H. Arthos, J. M. Binley, W, C, Olson, G. P. Allaway, C. Cheng-Mayer, J. Robinson, P.J. Maddon, and J. P. Moore. 1996. CD4-dependent, antibody-sensitive interaction between HIV-1 and its co-receptor CCR-5. Nature 384:184-187
11 Thomas M. Richardson, JR., Brenda L. Stryjewski, Christopher C. Broder, James A. Hoxie, John R. Mascola, Patricia L. Earl, and Robert W. Doms. 1996.Humoral response to oligomeric Human immunodeficiency virus type 1 envelope glycoprotein. Journal of Virology. Feb. 753-762
12 Yang, X., L. Florin, M. Farzan, P. Kolchinsky, P. Kwong, J. Sodroski, and R. Wyatt. 2000. Modifications that stabilize human immunodeficiency virus envelopeglycoprotein trimers in solution. Journal of Virology. 74:4746-4754
13 James D. Watson, Michael Gilman, Jan Witkowski, Mark Zoller, Recombinant HIV proteins may be effective as immunogen for AIDS vaccines. Recombinant DNA (2nd Edition):501-503
14 Olshevsky, U., E. Helseth, C. Furman, J. Li, W. Haseltine, and J. Sodroski. 1990. Identification of individual human immunodeficiency virus type 1 gp120 aminoacids important for CD4 receptor binding. Journal of Virology. 64:5701-5707
15 Barnett, S. W., S. Rajasekar, H. Legg, B. Doe, D. H. Fuller, J. R. Haynesm C.M. Walker, and K. S. steimer. 1997. Vaccination with HIV-1 gp120 DNA inducesimmune responses that are boosted by a recombinant gp120 protein subunit. Vaccine 15:869-873
16 Pantelis Poumbourios, Walid el Ahmar, Dale A. Mcphee, and Bruce E. Kemp. 1995. Determinants of Human immunodeficiency virus type 1 envelope glycoprotein oligomeric structure. Journal of Virology. Feb. 1209-1218
17 Patricia L. Earl, Christopher C. Broder, Deborah Long, Susan A. Lee, Jeffrey Peterson, Sekhar Chakrabarty, Robert W. Doms and Bernard Moss. 1994. Nativeoligomeric Human immunodeficiency virus type 1 envelope glycoprotein eilcits diverse monoclonal antibody reactivities. Journal of Virology. May. 3015-3026
18 Rogier W. Sanders, Linnea Schiffner, Aditi Master, Francis Kajumo, Yong Guo, Tatjana Dragic, John P. Moore and James M. Binley. 2000. Variable-loopdeletedvariants of the Human immunodeficiency virus type 1 envelope glycoprotein can be stabilized by an intermolecular disulfide bond between the gp120 and gp41 subunits. Journal of Virology. June. 5091-5100
19 James M. Binley, Rogier W. Sanders, Brian Class, Norbert Schuelke, Aditi Master, Yong guo, Francis Kajumo, Deborah. Anselma, Paul J, Maddon, WillimC. Olson, and John P, Moore. 2000. A recombinant Human immunodeficiency virus type 1 envelope glycoprotein complex stabilized by an intermoleculardisulfide bond between the gp120 and gp41 subunits is an antigenic mimic of the trimeric virion- associated structure. Journal of Virology. Jan. 627-643
20 Miroslaw K. Gorny, Thomas C. Vancott, Constance Williams, Kathy Revesz, and Susan Zolla-Pazner. 2000. Effects of oligomerization on the epitopes of the Human immunodeficiency virus type 1 envelope glycoproteins. Virology. 267:220-228