Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Effects of Bacillus spp. On Growth of Alfalfa ( Medicago sativa L. )

Bacillus속이 Alfalfa ( Medicago sativa L. ) 의 생장에 미치는 영향

  • Choi, Ki-Chun (College of Agriculture, Chonnam National University) ;
  • Youn, Chang (Dept. of Livestock Industry, Iri National College of Agriculture and Technology) ;
  • Chun, Woo-Bock (College of Agriculture, Chonnam National University)
  • 최기춘 (전남대학교 농과대학) ;
  • 윤창 (이리농공전문대학) ;
  • 전우복 (전남대학교 농과대학)
  • Published : 1997.09.30
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Abstract

This study was conducted to investigate the effects of antagonistic microorganisms, Bacillus spp., on growth of alfalfa(Medicag0 sativa L.) in repeated cultivation soil(RCS) and unrepeated cultivation soil(URCS). Alfalfa was established by seeding into pots 12 cm in diameter and 9 cm in depth containing 1 : 1 mixture of soil and vermiculite with antagonistic bacteria and pathogenic fungi. The growth experiment of alfalfa was conducted in pots in a vinyl house. The bacteria used in this study were Bacillus subtilis and hsants. B. subtilis was isolated and identified 60m forage rhizosphere soil and hsants isolated through cell fusion fiom B. subtilis 101 and B. thuringiensis. B. subtilis was named B. subtilis 101 and hsants named F -3 and F -8. From dark culture experimenf alfalfa was longer lived in treated soil with antagonistic bacteria than that in non-treated soil, and longer lived in URCS than that in RCS. However, alfalfa was shorter lived in RCS and URCS than that in autoclaved RCS. The number of leaves of alfalfa were positively affected by the inoculation of the antagonistic bacteria in both RCS and URCS. Dry weight of shoot and root was increased by the inoculation of the antagonistic bacteria(P< 0.05). However, the growth of alfalfa was decreased by the inoculation of the pathogenic hngi both RCS apd URCS.

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