• Title/Summary/Keyword: Culture Complex

검색결과 985건 처리시간 0.021초

Generation of Miniaturized Ovaries by In Vitro Culture from Mouse Gonads

  • Jang, Si Won;Choi, Hyun Woo
    • 한국발생생물학회지:발생과생식
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    • 제25권3호
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    • pp.173-183
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    • 2021
  • The incidence of infertility among individuals of reproductive age has been growing due to genetic and environmental factors, and considerable research efforts are focused on solving this issue. Ovarian development is an overly complex process in the body, involving the interaction between primordial germ cells and gonad somatic cells. However, follicles located in the center of the in vitro ovary are poorly formed owing to ovarian complexity, nutrient deficiency, and signaling deficiency. In the present study, we optimized methods for dissociating gonads and culture conditions for the in vitro generation of miniaturized ovaries. The gonads from embryos were dissociated into cell masses and cultured on a Transwell-COL membrane for 3-5 weeks. Approximately 12 follicles were present per in vitro ovary. We observed that miniaturized ovaries successfully matured to MII oocytes in vitro from 150 to 100 ㎛ gonad masses. This method will be useful for investigating follicle development and oocyte production.

A Study on the Combination of Manned-unmanned Teaming for Future Ground Combat Victory

  • Sung-Kwon Kim;Sang-Hyuk Park
    • International Journal of Advanced Culture Technology
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    • 제11권1호
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    • pp.159-164
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    • 2023
  • This study is for manned-unmanned teaming battles for future ground combat victories. The composition of the study is as follows. The introduction to Chapter 1 presents the necessity of this study from a macro perspective, Chapter 2, the review of the complex combat system for both manned and unmanned introduced the paradigm shift of the future battlefield and the cyber area that is superconnected to the network in future wars. Chapter 3 analyzed the combined combat system of manned-unmanned teaming in advanced military countries through the cases of the United States and Israel. In Chapter 4, after discussing the direction of the development of combat performance of the Korean Army, was concluded in Chapter 5. In other words, the purpose of this study is that as the concept of fighting artificial intelligence robots and military innovation changes, the method of performing battles must be changed in order for our military to win the battle.

The Painting Language of American Neo-Expressionist Painter David Sall

  • Bi Xin Zhi
    • International Journal of Advanced Culture Technology
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    • 제11권1호
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    • pp.192-197
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    • 2023
  • David Salle, born in Norman, Oklahoma in 1952, is the most important and controversial artist of the new-expressionist painting in the United States. His paintings are featured by unique artistic language, and extremely diverse and very ground-breaking expression, which reflect the American society at that time. Besides, he often uses complex image combination with striking colors and forms to attract and touch viewers. In this paper, based on the American new-expressionism, the painting language of David Salle's works was analysed, in which I found that it can be more intuitively and more profoundly feel the development of new-expressionist painting context and David Salle's particularity, and more clearly realize under what the characteristics of the era David Salle created the unique artistic language. The study on David Salle's unique painting language is of important research value and reference significance for our current artistic creation.

Comprehensive Approaches to Shoulder Impingement Syndrome: From Diagnosis to Rehabilitation

  • Jung-Ho Lee
    • International Journal of Advanced Culture Technology
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    • 제12권2호
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    • pp.90-97
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    • 2024
  • Shoulder impingement syndrome (SIS) is a common musculoskeletal condition characterized by pain and functional limitation due to the impingement of subacromial structures. This comprehensive review elucidates the complex nature of SIS, covering its pathophysiology, diagnostic methodologies, treatment options, and preventive measures. Through an exhaustive examination of current literature and clinical practices, the review highlights the importance of a multifaceted approach to SIS management. Physical therapy plays a pivotal role, focusing on exercises to strengthen shoulder musculature, enhance scapular stability, and improve range of motion. The review also discusses the strategic use of medications such as NSAIDs and corticosteroid injections, emphasizing their effectiveness in pain and inflammation management. Additionally, it advocates for structured rehabilitation programs post-treatment to restore function and prevent recurrence, recommending preventive strategies like ergonomic adjustments, targeted exercises, and proper technique training. This paper underscores the need for personalized and evidence-based treatment strategies, integrating physical therapy and pharmacological management when necessary.

Engineering Brain Organoids: Toward Mature Neural Circuitry with an Intact Cytoarchitecture

  • Hyunsoo Jang;Seo Hyun Kim;Youmin Koh;Ki-Jun Yoon
    • International Journal of Stem Cells
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    • 제15권1호
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    • pp.41-59
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    • 2022
  • The emergence of brain organoids as a model system has been a tremendously exciting development in the field of neuroscience. Brain organoids are a gateway to exploring the intricacies of human-specific neurogenesis that have so far eluded the neuroscience community. Regardless, current culture methods have a long way to go in terms of accuracy and reproducibility. To perfectly mimic the human brain, we need to recapitulate the complex in vivo context of the human fetal brain and achieve mature neural circuitry with an intact cytoarchitecture. In this review, we explore the major challenges facing the current brain organoid systems, potential technical breakthroughs to advance brain organoid techniques up to levels similar to an in vivo human developing brain, and the future prospects of this technology.

중·고등학생의 성장과정 상처와 열등감, 성격장애, 정신건강과의 관계 연구 (A Study on the Relationship of Wounds in the Growth Period, Inferiority Complex, Personality Disorder and Mental-Health among Secondary Students)

  • 이만표
    • 한국심리학회지 : 문화 및 사회문제
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    • 제18권4호
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    • pp.505-532
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    • 2012
  • 이 연구는 청소년학생들의 정신건강에 영향을 주는 성장과정의 상처, 열등감, 성격장애 간 관계를 분석하고자 하였다. 최종 분석대상은 중·고교생 2,119명을 표집, 조사·수집된 자료를 분석했다. 차이분석, 중다회귀분석, 경로분석 결과, 성장과정의 상처, 열등감, 성격장애, 정신건강 요인 면에서 여학생들과 고등학생들이, 그리고 가정경제 환경이 곤란한 학생들과 학업성적 수준이 하위권 학생들이 상대적으로 문제가 있음이 의미 있게 밝혀졌다. 열등감에 영향을 주는 성장과정의 상처 요인은 행복감, 안정감, 인정감의 상처 순으로 유의미하게 영향을 주는 것으로 확인되었고, 성격장애에 영향을 미치는 성장과정의 상처 요인은 행복감, 안정감, 자신감, 사랑, 평등감의 상처 순으로 유의미하게 영향을 주는 것으로 밝혀졌다. 또한 정신건강에 유의미하게 영향을 주는 성장과정의 상처 요인은 행복감, 안정감, 자신감, 사랑, 평등감의 상처 순으로 밝혀졌다. 그리고 정신건강에 미치는 관련변인들의 영향력은 전반적으로 성장과정 상처의 직접효과보다 성격장애와 열등감을 통한 간접효과가 높게 나타났다. 이는 성장과정의 상처가 열등감과 성격장애로 이어지고 궁극적으로 정신건강으로 악화될 수 있다는 교육적 시사점과 함께 가정환경의 건강성과 교육적 성장환경의 중요성을 일깨워 준다.

Investigation of the Microbiological and Biochemical Properties of Kimchi in the Submerged Model System Designed for Fermented Sausages

  • Lee, Joo-Yeon;Kunz, Benno
    • 한국축산식품학회지
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    • 제29권4호
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    • pp.423-429
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    • 2009
  • The objective of this study was to investigate the potential of the application of lactic acid bacteria (LAB) from kimchi as a starter culture in the production of fermented sausages. To achieve this, a submerged model medium that contained LAB as part of a complex system of kimchi (0.5, 1.0, 1.0, 3.0, and 5.0%) and lyophilized kimchi powder (0.2 and 0.5%) was fermented for 120 h. During the fermentation period, the growth of total viable organisms and LAB, and the changes in the pH and the titratable acidity, were investigated. The initial LAB counts ranged from 6.4 to 7.7 Log CFU/mL for the kimchi media, and from 6.9 to 6.9 Log CFU/mL for the kimchi powder media. In all the kimchi batches, the LAB increased logarithmically, and the highest LAB counts (around 9 Log CFU/mL) were reached in 24 h. An evident lag phase of the LAB was observed in the kimchi powder samples and reached 8.8 Log CFU/mL in 8 h. The decrease in the pH and the formation of lactic acid were rapid in the kimchi batches, and reached pH values of 3.4-3.5 in 12 h. With these results, the LAB that was integrated with the addition of kimchi or kimchi powder demonstrated its potential utility as a substitute for starter culture.

지하공간의 문화적 활성화를 위한 실내 환경계획요소의 분석에 관한 연구 (A Study on the Analysis of Elements of Interior Environmental Planning for Cultural Vitality of Underground Space)

  • 이효창;한정호;하미경
    • 한국실내디자인학회논문집
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    • 제19권5호
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    • pp.234-242
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    • 2010
  • In order to make an effective use of spatial resources in the city, detailed environmental plan strategies based on 'culture' are needed. An integral part of city spaces-'underground space' serves as one of the spatial resources with much effective usage potentials. Hence, 'underground space' needs cultural vitality. The purpose of this study is to propose an thorough indoor environmental plan guideline for the cultural vitality of 'underground space'. The methods used to conduct research include precedent study reviews and survey. Through this research, following conclusions are drawn. First, to promote cultural vitality at the 'underground space', it must be transformed into the cultural public place to be utilized for cultural activities by residents. Second, the 'underground space' requires 'concept of eco-friendly space for clean environment'. Third, 'safe environment' concept must be installed in the 'underground space' to promote the cultural vitality. Fourth, the 'underground space' requires 'complex/block level network plans between cultural spaces in addition to horizontal/vertical walking network between cultural environments in ground level and underground spaces. Fifth, the 'underground space' requires underground public cultural space plan through 'reasonable underground development with considerations of the facilities related to education, culture and history'. Sixth, 'public cultural space plan for various cultural spaces' and 'supply of space for cultural activities for residents and design plan for mutual culture exchange' are necessary.

Relationship Between Fractal Dimension and Morphological Features of Cephalosporium acremonium M25 in a 30-1 Bioreactor Culture

  • Lim Jung-Soo;Kim Jung-Mo;Kim Jong-Chae;Kim Chang-Ho;Yang Dae-Ryook;Chang Hyo-Ihl;Kim Seung-Wook
    • Journal of Microbiology and Biotechnology
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    • 제15권5호
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    • pp.971-976
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    • 2005
  • In a 30-1 bioreactor culture, whole differentiation occurred from 48 h, and then proceeded rapidly. As swollen hyphal fragments and arthrospores increased, cephalosporin C (CPC) production increased exponentially to $1.85\;g/1^{-1}$ at 72 h. To explain the morphological changes of Cephalosporium acremonium M25 more quantitatively, specific differentiation rates and fractal analysis were employed. Specific differentiation rates of morphological factors varied greatly during the period of culture time from 48 h to 72 h, when CPC production increased significantly. Changes of fractal dimensions showed a pattern similar to that of the specific rate of arthrospores. Furthermore, it was inversely related to the specific rate of tips. Overall, it was suggested that the fractal dimension had potential for a new morphological parameter of fungal morphology, showing complex differentiation patterns.

Quantitative Polymerase Chain Reaction for Microbial Growth Kinetics of Mixed Culture System

  • Cotto, Ada;Looper, Jessica K.;Mota, Linda C.;Son, Ahjeong
    • Journal of Microbiology and Biotechnology
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    • 제25권11호
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    • pp.1928-1935
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    • 2015
  • Microbial growth kinetics is often used to optimize environmental processes owing to its relation to the breakdown of substrate (contaminants). However, the quantification of bacterial populations in the environment is difficult owing to the challenges of monitoring a specific bacterial population within a diverse microbial community. Conventional methods are unable to detect and quantify the growth of individual strains separately in the mixed culture reactor. This work describes a novel quantitative PCR (qPCR)-based genomic approach to quantify each species in mixed culture and interpret its growth kinetics in the mixed system. Batch experiments were performed for both single and dual cultures of Pseudomonas putida and Escherichia coli K12 to obtain Monod kinetic parameters (μmax and Ks). The growth curves and kinetics obtained by conventional methods (i.e., dry weight measurement and absorbance reading) were compared with that obtained by qPCR assay. We anticipate that the adoption of this qPCR-based genomic assay can contribute significantly to traditional microbial kinetics, modeling practice, and the operation of bioreactors, where handling of complex mixed cultures is required.