Early development of porcine oocytes fertilized in vitro was examined in different culture conditions. Porcine ovaries were collected from local slaughter-house. Cumulus-oocytes complexes were aspirated from 2 to 6 mm follicles. The collected oocytes were cultured for in vitro maturation in NCSU-23 medium with 5 mM hypotaurine, 0.57 mM cystein, 10% porcine follicle fluid, 10 IU/ml PMSG and 10 IU/ml hCG for 42~44 hrs. The frozen-thawed spermatozoa were washed by centrifigation 2 times at 1, 500 rpm in D-PBS with 5.56 mM glucose, 0.33 mM Na-pyruvate, 100 IU/ml penicillin, 1$\mu\textrm{g}$/ml streptomycin and 1ng/ml BSA. The fertilization medium used mTBM with 2 mM caffeine and 2 mg/ml BSA and adjusted to a pH of 7.2 to 7.4. The final concentration of spermatozoa was adjusted to 2.5$\times$10$^{6}$ cells/ml motile sperm during fertilization in vitro. At 8hrs h after insemination, the oocytes were transferred into NCSU-23 medium with 5.0 mM hypotaurine and 4 mg/ml BSA and cultured for 7 days. In first experiment, the mean numbers of oocytes collected from 20 ovaries were 674.4 oocytes, and 4.1(27.6), 12.5(84.0), 25.4(171.6) and 57.9%(390.8) for A, B, C and D grade in morphological classification. In the second experiment, when culture medium was supplemented with various concentrations of EGF, the proportions of oocytes cleaved were 56.9, 55.7, 61.9 and 54.7% for 0, 5, 10 and 20ng/ml EGF. The higher proportions(15.1%) of oocytes developed to morular stage were obtained at concentration of 10ng/ml than 0 and 5ng/ml EGF (P<0.05). However, the proportions of embryos developed to blastocyst stage were not significantly different among concentrations of EGF. In another experiment, when the medium supplemented with catalase was used, the proportions of oocytes cleaved were higher in the concentration of 0 unit (56.5%, 61/108) than 100 and 1, 000 unit/ml of catalase (P<0.05). Although the developmental capacity of embryos was improved by medium with 0 unit/ml compared with 100, 500 and 1, 000 units/ml of catalase in oocytes developed to morula and blastocyst stages, were not significantly different among concentrations of catalase.
SHIN, AYOUNG;KIM, DONGSUNG;KANG, TEAWOOK;OH, JE HYEOK
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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v.25
no.2
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pp.26-41
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2020
In order to culture a life for the physiological and ecological research of the meiofauna, this study aimed to identify the most ideal condition in which the meiofauna can be cultured within a laboratory by setting various environmental conditions. The sediment deposits and seawater were collected from the intertidal zone in Mallipo of the west coast. A aquarium in which the internal environment can be controlled by constantly maintaining the temperature and humidity was fabricated and the culture experiments of the collected meiofauna were conducted together with the sea water and sediment deposits collected. The experiment 1 was conducted after establishing the similar environment as the collecting location. Under the same condition as the experiment 1, the experiment 2 verified a difference between when live foods were supplied and were not. In the experiment 3, the changes in the meiofauna colony were checked according to with or without light and live foods. In the results of culturing experiments, the habitat density and the number of appeared classification groups of the meiofauna colony were relatively higher both in the water tank with supplying the live foods and under the condition of having light in 12-hour cycle than those in the aquarium without live foods and under no light condition. In addition, the habitat density of meiofauna cultured within a laboratory exhibited relatively higher value than that under the natural state.
Today, as individuals show their social identities and reflect their being as the members of society with a culture, an art style and communication function are stood out in fashion photographs. Accordingly, the meanings of images into text are expanded in its interpretative width through the acceptor's various terms. This researcher looked into four theories of both positions on the textuality of language and image, and considered the point of discussion on image of each theory through modern fashion photographs. First, the theory which divides language and image as auditory and visual recognitions in the textuality of language and image is limited from the view it focuses on only one side without considering the ambivalent elements of each field. For the textuality in modern fashion photographs, the observer attempts to turn it into text to give meaning to it as the recognition through five senses conforming to the acceptor's condition. Second, the theory dividing language and image into the text of time properties and spacial properties has limitation in the text, for acceptor's experience of the object appears as the structured form in time and space rather than being defined as two things like time and space. Third, the theory classifying the language and image text into conventional taste and natural taste has limitation from the view that image text is hardly an object of consistent classification in ease of recognition by the code accepted in society. Thus, this can't be fundamental approach for the understanding of the text of decoding trend represented in modern fashion photographs. Fourth, accordingly, this researcher focussed on contextual and arbitrary text of fashion photographs through the theory of Nelson Goodman which discusses image text through the differences in textuality. Basic mechanism of perceiving and recognizing and distinguish image is closely related to habit and custom like language. So, each acceptor perceives the image as a text through arbitrary interpretation obtained by individual, empirical, historical, and educational viewpoints. The textuality of modern fashion photographs aims to widen the range of diverse knowledge and understanding, transcending the regulations of simple function of existing fashion photographs. Consequently, this researcher puts forward the opinion of consistent and diverse follow-up studies on instilling meaning into fashion photographs for the understanding de-regulatory and de-constructive through various senses by avoiding only one sense-dependent fixed and regulatory properties of it.
Journal of the Korean Institute of Landscape Architecture
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v.38
no.1
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pp.107-118
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2010
Palaces of a country can be representative aggregate of the culture and arts of the country. Palaces were used not only as a living place to protect kings and royal families and to live cultural lives, but also a political place to govern the country. Kings in Choseon founded the country on the Sung Confucianism, their philosophical background. They built Bangji and Wondo as constant reminders of their philosophy. Bangji is the most apparent characteristic in the palace gardens of the Choseon Dynasty. Finding out the original form of Jidang, restoring the original and passing it on to future generations can be passing on the spiritual culture of our ancestors. This study is done to find out or locate well preserved Jidang, transformed Jidang and lost Jidang in Changdeokgung, which is the best conserved palace of Choseon. This study is composed of Jidang which has been kept in its original state, transformed Jidang, locating lost Jidang and Jidnang which has been unearthed. The total number of Donggwoldo's Jidangs is 17; those that are kept in their original state, 4; transformed Jidangs, 5; Jidangs that were lost and have been located, 8; and newly appeared Jidangs, 2. From the results, opinions on restoration are presented. This study can be a small drop in the thin stream of tradition passing onto future generations in this world where knowledge and information are transported momentarily and the classification of culture and border is mixed, yielding vagueness.
Among the cultural industries, the game industry is the most economically valuable industry. It has been about twenty years since the game policy has been implemented and the game laws have been enacted. If the law is a willing expression for the realization of the policy, the orientation of the game policy can be grasped through revision of the game laws. SOUND RECORDS, VIDEO PRODUCTS, AND GAME SOFTWARE ACT, established in 1999, and GAME INDUSTRY PROMOTION ACT, which was enacted in 2006, are regulated by many revisions. In this paper, I try to understand the direction and meaning of Korean game policy(classification, game dysfunction, gambling, industry growth) through the contents of the revision of the game law for 20 years. The game policy shown through the amendment of the game law is intended to protect the game by regulating the game, and to protect the game user by preventing the gambling and preventing the game dysfunction, and to increase autonomy of users and choice of producers by switching to self rating system, and based on this, an environment for continuous industrial growth is created. In the future, game policies should consider cooperation with social areas beyond game-specific areas. On the other hand, it needs to respond to new agendas such as polarization of industrial structure, fair environment, employment environment.
The exterior color of the building reflects its style. With the advancement of the 'Village Art' project in South Korea, many villages have gradually developed into tourist attractions. Therefore, it is also important to distinguish between residents' lifestyles and tourist needs through architectural color design. This article first investigates the literature and analyzes the use function of the building and the role of the exterior color in the villages. Secondly, the four types of buildings in Huinnyeoul Culture Village: residential, commercial, leisure, and public toilets are investigated as the research objects. Analyze through the KSCA color measurement analysis system and questionnaire survey, and propose a color plan for the exterior of the building. Research results: First, the main color area of the outer wall of the residential building should be the largest, with a tendency of high brightness and low saturation. Second, small buildings with high chroma in the recreation area can be used as visual guidance.Third, the buildings in the commercial area are mainly of high brightness, high chroma, and multi-hue. Fourth, the main color of the bathroom is the main color of the residential area and the leisure area, which can provide a sense of visual stability.
Journal of Korea Entertainment Industry Association
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v.14
no.6
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pp.125-137
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2020
The purpose of this study is to analyze new words in Korean and Chinese based on changes in popular culture. As China and Korea embrace increasingly close communication in recent years, their languages have influenced each other. A lot of new Korean and Chinese words have been discovered to have the same linguistic characteristics. New words are considered as new developments of a language. They are welcomed and widely used by young people in Korea and China. Therefore, in terms of the communicative function of languages, it is worthwhile to understand new words in Korean and Chinese from the perspective of academic research. This study takes Chinese words created in 2018 as the research object. Firstly, a morphological and semantic comparison of Chinese words created in 2018 and those created in 2017 is carried out to extract the characteristic indicators of Chinese words created in 2018, with emphasis on compound words, abbreviations, substitutions, patters and rhetorical expressions. Secondly, the similarities and differences of these Chinese words with Korean words created in 2018 in terms of morphology are analyzed. Finally, after conducting sample classification and comparison, the characteristics of new Chinese and Korean words and the interaction mechanism under mutual influence are concluded. According to the study, the majority of the new words are created on the basis of existing words. Thus, it is important to explore the morphology of new words as a standard language.
Presenting symbolic concepts consistent with the culture of the host country and international trends at international events will win the favor of the world and raise the image of the host country. The international event mascot symbolically represents the host country's unique culture, and is a good means to enhance the sense of belonging and pride of its members and to display the image of the host country in an outwardly. This study discussed the symbolic elements of the host country characteristics reflected in FIFA's World Cup event mascot. A total of 14 mascots of World Cup events were held from 1966 to 2018, and their materials can be divided into animals, plants, people and creations. The mascot was applied with the characteristic elements of regional specialties, the flag of the host country, symbolic attire, language of the hosting area, social issues and the mascot's dress, posture, props and expression characters of soccer events were used as symbolic elements. First of all, the implications of the research were that mascots were more strongly expressing the "football" signifying element, the theme of events, rather than regional characteristics. Second, the use of 'national flag' was highlighted among the elements of expressing regional characteristics. Third, 'animal' was preferred for mascot material. Fourth, mascots have become integrated with 'cultural perfumes' and play an extended role in raising social awareness. Implications derived from the classification of characteristics and symbol representation elements raised in this study will be used as a basis for the planning of international event mascots.
The purpose of this study was to find out the impact of psycho-social factors (social relationship and personal) as well as illness history and economic status on physical health and subjective well-being among the retired Koreans elderly. Data were collected from 1,315 elders (mean age = 72.70yrs) residing in Seoul and Chuncheon regional area via interviews(Time 1), and them were re-interviewed two year later(Time 2). Multiple regression analyses indicated that the retired elders' illness history, economic status, marital satisfaction, fulfillment of self-esteem need, drinking behavior, positive affectivity, negative affectivity and physical health to predict their subjective well-being at Time 1(R2=.705). The retired elders' economic status, marital satisfaction, positive affectivity, negative affectivity and physical health to predict their subjective well-being at Time 2(R2=.418). The retired elders' illness history, economic status, expectations for one's offspring, drinking behavior and subjective well-being to predict their physical health at Time 1(R2=.364). And the retired elders' illness history, economic status, marital satisfaction, positive affectivity and negative affectivity to predict their physical health at Time 2(R2=.265). In case of retired elderly, suggested for the psycho-social determenants of subjective well-being and physical health by occupational classification. The implications of this study and the suggestions for furture study were discussed.
A physical characteristics and chemical compositions change by weathering on the granite were examined for the conservation treatment of the Mireuksaji temple stone pagoda. The natural weathered granite was collected from the Mt. Mireuk, and divided into the classification standards based on weathering degrees and strength measured by rock-test hammer. The results from comparison of the strength measured by undestructive rock-test hammer and the strength values converted from ultrasonic velocity showed that each strength measurement value was proportionate. The water absorption of the sample was 1.68 to 0.20%. The F-type of fresh rock was not naturally saturated and the WW-type was naturally saturated but took quite a long time. The water absorption was increased gradually in order of SW-type, the MW-type and the HW-type according to weathering condition. The CW-type samples showed the highest water absorption among the weathered classification samples. Through dyeing test, it was found out that only the feldspar was dyed out of the F-type and the WW-type. The SW-type and the MW-type were distinguished by the fact that plagioclase being dyed. And dyed area was expanded to quartz crack in HW-type and CW-type. Physical change by weathering of the rock-forming minerals could be classified with 3 grades. Through the XRD analysis, albite among the rock-forming mineral showed remarkable decrease. SEM-EDX analysis of the component change in the rock-forming minerals such as biotite, plagioclase, and orthoclase, showed that in case of highly-weathered grade samples compared with fresh samples, contents of the $Al_2O_3$, $K_2O$, $Na_2O$ increase and CaO, MgO decrease in the biotite, the CaO, $K_2O$ increase and $Na_2O$ decrease in the plagioclase, the $Al_2O_3$ a little increase and $K_2O$, $Na_2O$ decrease in the orthoclase. The results of extracted cation analysis using the powder samples of each weathering grade, the CaO, $Na_2O$, $K_2O$ and MgO are highly chemical variations in rock forming minerals and positive variation show high in the weathering grade of the WW-type and CW-type. This research will be used as an importance data to establish a plan for conservation treatment of composed stone in the Mireuksaji temple stone pagoda.
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