• Title/Summary/Keyword: Cultural and morphological characteristics

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Characterization of a Brown Rot Fungus Isolated from Dwarf Flowering Almond in Korea

  • Shim, Myoung-Yong;Jeon, Young-Jae;Kim, Seong-Hwan
    • Mycobiology
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    • v.35 no.1
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    • pp.30-35
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    • 2007
  • The fruits showing brown rot symptom on dwarf flowering almond were found in Gongju, Chungchungnam-Do in Korea in July 2005. Small water-soaked lesions on the fruits were initiated, and gradually developed to soft rot covered with gray conidia. Then the diseased fruits were shrunk and became grayish-black mummies. A fungus was isolated from the diseased fruit and its morphological, cultural and molecular genetic characteristics were investigated. Typical blastospores of Monilinia spp. were observed under a light microscope both from tissues of the diseased fruits and from PDA-grown cultures. The fungus grew well at $25^{\circ}C$ and on PDA. The ITS ribosomal DNA region (650 bp) of the fungus was amplified by PCR and analyzed. Comparative data on ITS sequence homology among Monilinia spp., ITS sequence-based phylogram and morphological characteristics showed that the fungus is Monilinia fructicola. This is the first report on Monilinia fructicola causing brown rot on fruits of dwarf flowering almond in Korea.

Taxonomical Characteristics of Xylaria spp. Collected from Malaysia

  • Lee, Yang-Soo;Han, Sang-Sub;Jeong, In-Soo
    • Mycobiology
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    • v.30 no.4
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    • pp.193-196
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    • 2002
  • Five species of the genus Xylaria are recognized on Malaysia materials: X. hypoxylon(L. FR.) Grev, X. allantoidea(Berk.) Fr., X. cubensis(Mont.) Fr., X. curta Fr. and X. grammica(Mont.) Fr.. The collection of Xylaria allantoidea from Malaysia is compared to the collection from Taiwan in morphological characters. The species are described their the host and cultural characteristics by a bright microscope and a scanning electron microscope.

Cultural and Morphological Characteristics of a New White Button Mushroom Cultivar 'Saedo'

  • Oh, Youn-Lee;Jang, Kab-Yeul;Kong, Won-Sik;Shin, Pyung-Gyun;Oh, Min ji;Choi, In-Geol
    • The Korean Journal of Mycology
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    • v.44 no.2
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    • pp.94-99
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    • 2016
  • Since development of the first Korean button mushroom cultivar 'Sae-Ah' in 2010, mushroom farmers have demanded an improved cultivar. Although a new cultivar, 'Saejeong', was developed in 2011, this cultivar has thus far been difficult to cultivate in typical mushroom farms. To solve this problem, another new cultivar, 'Saedo', was developed in 2012. This new cultivar was selected by genetic analysis from crossing the heterokaryon line A175 and the homokaryon line ASI1346-15. The heterokaryon A175 was a crossed line between strains ASI1038-211 and ASI1346-20. The mycelium of 'Saedo' showed good growth on compost dextrose agar at $25^{\circ}C$. The morphological traits such as the pileus (cap) and stipe of the 'Saedo' cultivar were thicker than those of the reference control 'Saejeong', and the production yield was increased by 1.89-fold compared to that of the reference control. A field experiment of the 'Saedo' cultivar has been ongoing since 2013. The yield of the 'Saedo' cultivar was found to be substantially higher than that of the reference control during the $2^{nd}$ flush.

Cultural and Pathogenic Characteristics between Colletotrichum gloeosporioides and Glomerella cingulata Isolated from Strawberry in Korea (국내 딸기 탄저병균 Colletotrichum gloeosporioides와 Glomerella cingulata의 배양적, 병원학적 특성)

  • 남명현;정석기;유성준;서관석;김홍기
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.654-660
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    • 1998
  • Colletotrichum gloeosporioides and Glomerella cingulata are the most important pathogens causing anthracnose which may reduce the stand rate and yield on wide kinds of plants including strawberry. Average occurrence rate of anthracnose is 36.9% on major strawberry cropping areas in Korea. We newly found that C. gloeosporioides which does not forming a sexual stage, infects strawberry and differs in some characteristics concerning virulence, cultural and morphological properties to G. cingulata which has a sexual stage. C. gloeosporioides was mainly isolated from the crown with 35.2% rate, while G. cingulata was largely isolated from petiole, runner with 40.9% rate in infected strawberry plants. These two pathogens showed significant differences in cultural characteristics such as perfect stage formation, temperature response as well as benomyl resistance. It was demonstrated that C. gloeosporioides has significantly stronger pathogenicity than G. cingulata in pathogenicity test carried on strawberry plants to various strawberry cultivars. Akihime, Akaneko and Nyoho forcing cultured strawberry cultivars, considered to be susceptible, while semiforcing cultured cultivars, such as Suhong and Holowase, were shown resistant to both pathogens. In non-wound inoculation, C. gloeosporioides was shown pathogenicity on the apple fruit, but G. cingulata could not infect it.

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Genetic Diversity of Pleurotus eringii Strains in Korea Based on Morphological Characteristics and PCR Polymorphism (형태적 특성과 PCR다형성 분석에 의한 국내 큰느타리버섯 계통의 유전적 다양성 분석)

  • Jeon, Sun-Jeong;Kim, Jong-Kun;Kim, Gum-Hee;Chi, Jeong-Hyun;Seo, Geon-Sik;Kang, Hee-Wan
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.19-27
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    • 2009
  • This study was conducted to investigate genetic characteristics of 25 Pleurotus eringii strains that have been released in Korea based on cultural, morphological features and PCR fingerprints. Strains PER-007 and PER-012 showed distinct cultural characteristics in growth rate, morphological characteristics of mycelial colony and fruiting bodies when compared to those of other strains. Strain PER-007 did not form primordium initiation in sawdust medium and PER-012 also showed different phenotypes on fruiting bodies. Eleven URP primers were used to detect PCR polymophic bands in P. eringii strains. Primers URP1F, URP2R, URP2F, URP4R, URP6R, URP9F and URP17R were selected as useful primers for amplifying PCR polymorphic bands in P. eringii strains. The genetic similarity index was calculated by using PCR polymorphic bands amplified by eight URP primers among the 25 strains. The P. eringii strains were grouped by four distinct clusters on the UPGMA analysis. The genetic similarity values ranged from 100% to 76% were observed in three major groups, suggesting close genetic relatedness of them. Exceptionally, PER-007 and PER-017 were involved in outgroup.

Evaluation of Characteristics of Wax-treated Paper Depending on Coating Methods (도포방식에 따른 밀랍지 시제품의 특성 분석)

  • Seo, Jin-Ho;Choi, Kyoung-Hwa;Park, Ji-Hee;Kang, Yeong-Seok;Yoon, Kyoung-Dong
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.41 no.2
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    • pp.64-69
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    • 2009
  • Cultural heritages represent the histories and intellectual level of a country. The Annals of Joseon Dynasty are regarded as the most valuable resources containing the record of Korean history. In some periods, wax-treated paper was used as the material of the Annals of Joseon Dynasty. Many previous studies have focused on the development of paper used as the material of the Annals of Joseon Dynasty, but the manufacture method of wax-treated volumes and the degradation mechanism of wax have been not fully understood yet. The purposes of the present study are to produce various kinds of wax-treated paper using by different coating methods and to analyse the morphological properties of wax-treated paper. The analysis of SEM demonstrates that beeswax treated paper made by brushing method produces flatter surface and more excellent penetration. Wax layer is produced on the cross section of beeswax treated paper as the increase of coating amount.

Effect of pHs on Morphological and Cultural Characteristics of Alkalophilic Coryneform Bacteria TU-19 (호알칼리성 Coryneform Bacteria TU-19의 형태적, 배양적 특성에 미치는 pH효과)

  • Choi, Myoung-Chul;Yang, Jae-Sub;Hwang, Cher-Won;Kang, Sun-Chul
    • Applied Biological Chemistry
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    • v.41 no.5
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    • pp.337-341
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    • 1998
  • The morphological and cultural characteristics of alkalophilic Coryneform bacteria TU-19 were investigated at various pHs. This bacterium showed normal growth pattern at $pH\;9.0{\sim}10.0$, but the cell growth was completely inhibited at extreme pH (12.0 or more). Interestingly, at pH 8.0 the morphology of the bacterial cells seems to form convoluted filaments during the exponential growth phase while at pH 10.0, the optimal pH for the growth of this organism, the bacteria grew with variable paired or single forms, and straight rods during growth stages. Growing in alkaline media $(pH\;9.0{\sim}11.0)$, it adjusted the pH of the culture media to around pH 8.5 by itself.

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Research and Development of Cultural Products, Cultural Heritage has been Applied Design Elements Bekuje - Focusing on the pattern Jinmyosu - (백제권 문화재 디자인 요소를 적용한 문화상품 개발연구 - 진묘수 문양 중심으로 -)

  • Kim, Chung-Ho;Kang, Ho-Yang
    • Journal of the Korea Furniture Society
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    • v.23 no.3
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    • pp.252-260
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    • 2012
  • Cultural products, applying the design elements of cultural heritage Bekuje content of the product, we chose the useful material that can be used on a desk stand in the smart-phone Sticky Notes (Post-it) for use in modern life. The goal of the development design, developed in the design and excavation element modeling can be taking advantage of the characteristics of traditional decor come connected by a pattern of Baekje, eggplant in harmony with modern life, a new sense was harmony, design. He stressed the beauty of decorative molding to apply discriminatory morphological changes associated with each, a unique pattern of human pattern Jinmyosu Bekuje was elected to the motif of cultural property. You can also use a visual representation of the beauty obviously sophisticated pattern that appears frequently in Baekje, was also emphasized inheritance and traditions of Korea. We also attempted a new way to make sure we can diversify and whether it is possible as a new form, enhance the value of products, from concept to molt general existing tourism products.

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Taxonomical studies of Korean aspergilli (한국산 Aspergilli에 관한 분류학적 연구)

  • 김상재
    • Korean Journal of Microbiology
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    • v.9 no.1
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    • pp.1-26
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    • 1971
  • Intensive taxonomical studies of the Aspergilli have long been made. Altogether 132 species and 18 varieties are recognized in the book "The Genus Aspergillus" written by Raper and Fennell (1965), in contrast to 77 species, 8 varieties and 4 mutations in " A Manual of Aspergilli" written 20 years earlier by Thom and Raper (1945). Classification of the Asperilli by Thom and Raper (1945) and by Raper and Fenell (1965). Classification of the Aspergilli by Thom and Raper (1945) and by Rapher and Fenell (1965) have been based mainly upon morphological and cultural detail both physiological and biochemical activities. In Korean there are many kinds of foods fermented natrually without the employment of selected microorganisms, and there are, of course, many different microorganisms serving in the fermentation fermented foods than other countries, the distribution and biological properties of the Asperigilli, in Korea are more variable. Taxonominical studies with 36 strains of Asperilli were based upon the examinations of morphological, cultural, and physiological characteristics. nineteen strains indigenous to Korea were selected from a lot of strains which had been isolated from meju and kokja and one strain from soil. They were identified according to the group key of Raper and Fennell. Ten strains were donated by Dr.Hesseltine of the Northern Utilization Research and Development Division in the U.S.A. From the Asp. japonicus supplied by Dr.Hesseltine, a white mutant was isolated and also studied. Two strains were donated by Dr. Murakami of the Research Institute of Brewing in Japan, and four strains came from Korean industrial companies.ndustrial companies.

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Morphological, Molecular and Pathogenic Characteristics of Botrytis elliptica and Botrytis cinerea Associated with Leaf Blight of Lily (백합 잎마름병에 관여하는 Botrytis elliptica와 Botrytis cinerea의 형태적, 분자적 특성과 병원성)

  • Kim, Won-Ki;Park, Myung-Soo;Hahm, Soo-Sang;Yu, Seung-Hun
    • The Korean Journal of Mycology
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    • v.35 no.2
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    • pp.68-75
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    • 2007
  • The seventy nine isolates of Botrytis spp. were obtained from leaf blight lesions of lily in Korea, Japan and Netherlands. Morphological and cultural characteristics of them were investigated and molecular characteristics of them were determined using sequence analysis of RNA polymerase II (RPB2) and heat-shock protein 60 (HSP60) gene. A selection of Botrytis isolates were evaluated for their pathogenicity to lily. Based on morphological and cultural characteristics, the Botrytis isolates were divided into two groups, and identified as B. elliptica (n = 54) and B. cinerea (n = 25). Based on analysis of RPB2 and HSP60 sequences, the Botrytis isolates were also divided into two groups and well supported morphological groupings. Spore suspensions of B, elliptica showed significant pathogenicity on lily leaves and flowers, however those of B. cinerea showed pathogenicity only on flowers but not on leaves. The latter showed pathogenicity on lily leaves only when spore suspensions amended with PDB were used as inocula.