The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Morphological, Molecular and Pathogenic Characteristics of Botrytis elliptica and Botrytis cinerea Associated with Leaf Blight of Lily

백합 잎마름병에 관여하는 Botrytis elliptica와 Botrytis cinerea의 형태적, 분자적 특성과 병원성

  • Kim, Won-Ki (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Park, Myung-Soo (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Hahm, Soo-Sang (Taean Lily Experiment Station, Chungnam Agricultural Research and Extension Services) ;
  • Yu, Seung-Hun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 김원기 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 박명수 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 함수상 (충남농업기술원 태안백합시험장) ;
  • 유승헌 (충남대학교 농업생명과학대학 응용생물학과)
  • Published : 2007.12.31
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Abstract

The seventy nine isolates of Botrytis spp. were obtained from leaf blight lesions of lily in Korea, Japan and Netherlands. Morphological and cultural characteristics of them were investigated and molecular characteristics of them were determined using sequence analysis of RNA polymerase II (RPB2) and heat-shock protein 60 (HSP60) gene. A selection of Botrytis isolates were evaluated for their pathogenicity to lily. Based on morphological and cultural characteristics, the Botrytis isolates were divided into two groups, and identified as B. elliptica (n = 54) and B. cinerea (n = 25). Based on analysis of RPB2 and HSP60 sequences, the Botrytis isolates were also divided into two groups and well supported morphological groupings. Spore suspensions of B, elliptica showed significant pathogenicity on lily leaves and flowers, however those of B. cinerea showed pathogenicity only on flowers but not on leaves. The latter showed pathogenicity on lily leaves only when spore suspensions amended with PDB were used as inocula.

본 연구는 백합 잎마름병에 관여하는 B. elliptica와 B. cinerea의 형태적, 배양적 특성을 조사하고, 유전자 염기서열에 기초한 분자계통분석을 하며 병원성을 비교하기 위하여 실시하였다. 한국, 일본, 네덜란드의 주요 백합 재배지역에서 잎마름병에 걸린 백합으로부터 79개의 Botrytis속 균주를 분리하였다. 분생자경과 분생포자의 형태적 특징에 의하여 Botrytis 균들은 2개의 그룹으로 분리되었으며 이들은 각각 B. elliptica와 B. cinerea로 동정되었다. 균사생장 속도는 B. cinerea가 B. elliptica에 비해 빨랐으며 이러한 형태적, 배양적 특성에 기초하여 79개의 균주 중 54균주(68%)가 B. elliptica로, 25균주(32%)가 B. cinerea로 동정되었다. RPB2와 HSP60의 염기서열 분석을 통해, 공시균주는 두 그룹으로 구분되었으며 이들은 형태적으로 동정한 그룹과 일치하였다. 포자 현탁액을 이용한 B. elliptica와 B. cinerea의 병원성 검정 결과 B. elliptica 균주들은 백합 잎과 꽃잎 모두에 병원성을 나타내었다. 그러나 B. cinerea 분생포자 현탁액은 꽃잎에만 병원성을 나타내었으며 PDB가 첨가된 포자현탁액을 접종원으로 사용했을 때에만 백합 잎에 병원성을 나타내었다.

Keywords

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