• Journal of Mushroom
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• v.11 no.3
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• pp.154-158
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• 2013

To develop a new variety of oyster mushroom(Pleurotus ostreatus), parental strains was selected by the method of Mon-Mon crossing between monokaryotic strains derived from ASI 2596(Suhan No.3) and ASI 2782(Black pileus mutant). The SB-73(ASI 2596-11 x 2782-8) was shown the best cultural characteristics, selected to be a new variety and named as 'Dagul'. The 'Dagul' was formed incompatibility line distinctly in the confrontation growth of parental strains Suhan No.3 and ASI 2782. The optimum temperature for mycelial growth, fruiting body development and pH arrange were $25{\sim}30^{\circ}C$, $14{\sim}17^{\circ}C$ and pH5~8, respectively. Fruiting body production per bottle was about $68.0{\pm}24.1$ g which is almost 115% quantity compared to that of other variety Suhan No.3. And also the stipe is long and individual generation is multiple. Analysis of the genetic characteristics of the new variety 'Dagul' showed different DNA bands as that of the control strains, Suhan No.3 and ASI 2782, when RAPD(Random Amplified Polymorphic DNA) primers URP7 and Rcb1 were used. This new variety 'Dagul' of oyster mushroom is characterized by multiple of individual generation and the stipe is long. We therefore expect that this new strain will increase of the income by cultivation of field.

• Journal of the Korean Institute of Landscape Architecture
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• v.42 no.6
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• pp.72-88
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• 2014

This study was conducted in order to analyze user behavior and preference factors in the outdoor spaces of mental hospitals. Among hospitals with 250 or more beds, 5 hospitals were selected in consideration of size of garden and diversity of garden elements. The subject of the study was restricted to mild cases of schizophrenia while 30~50 patients were selected on the recommendation of their doctor from 5 hospitals, respectively. The physical environment was analyzed, focusing on space components, after visiting the sites of study. A face to face interview method was selected in consideration of patients' cognitive abilities, a total of 230 questionnaires were used for the analysis. The results of the study can be summarized as follows. Rest facilities occupy the largest numbers in the components of garden, and those are followed by landscape facilities, walking/exercise facilities, and experience facilities. Outdoor walking/exercise programs are classified into group walks and free walks with most patients taking group walks. Most of the patients visit these outdoor spaces every day but some of them rarely use the outdoor areas. In order to increase the efficiency of using these outdoor spaces, the percentage of space for ensuring a sense of control should properly harmonize with the percentage of space to facilitate patients in having social contact. With regard to the reasons for preferring the most widely-used outdoor spaces, landscape/environment property was the most important, followed by functionality and then accessibility. Major activities in the preferred space are mainly composed of walking/exercise and rest. The preferred facilities are waterscape facilities such as ponds, waterfalls and fountains, rest facilities such as pergolas and shade trees, and lawn. It was understood that naturalness should be considered to be the most important factor in constructing a new healing garden, followed by aesthetics and amenities. Single facilities rated by preference for introduction were flower beds, trails, and lawn. According to type, waterscape facilities such as fountains, ponds, waterfalls and waterwheels were most preferred. Space for natural distraction and programs for the cultivation of flower beds were also preferred. The ideal image of a healing garden should be bright, familiar, and orderly as a whole, having plenty of introduced facilities. Open spaces were preferred to enclosed spaces. Finally, the image of a garden that helps patients feel calm was thought to be that of the most ideal garden.

• Journal of the Korean Society of Radiology
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• v.4 no.1
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• pp.31-35
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• 2010

Recently, the incidents of direct or indirect radiation exposure due to increase of use of radiation or radioisotope are on the increase in medical and industrial circles. If cells are irradiated, free radicals are created through biological process, and cells are directly or indirectly damaged. This research intends to explore into the effect of saponin at the level of cell (in vitro) and entity (in vivo), using red ginseng extract "saponin", as radioprotective agent. In the experiment implemented at the level of cell (in vitro), degree of cell activity was measures by adding mouse mesenchymal stem cells "C3H/10T1/2 cells" into red ginseng extract "saponin(0, 0.05, 0.2, and 0.4 g/L)", and then the optimal concentration of saponin influencing cells was calculated, in 24, 48, 72, and 96 hours after gamma irradiation at the optimal concentration of saponin, each cell survival rate was observed through XTT assay. The best time period of cultivation for the optimal activity of C3H/10T1/2 cells was as 48 hours, and the degree of optimal activity was shown at 0.05 g/L. In 48 hours after irradiation of 5 Gy to C3H/10T1/2 cells at 0.05 g/L, the degree of activity of cells increased by 10%. In the experiment implemented at the level of entity (in vivo), red ginseng extract "saponin" at a dose of 100 mg/kg/day was injected into the abdominal cavity of six-week immature mouse for two weeks. Right after the last abdominal injection, total body irradiation of gamma rays was carried out at a dose of 5 Gy and 10 Gy. And after irradiation, the blood sample was taken, and then the number of red corpuscles was counted. In result, the decrement of experimental group treated with red ginseng extract "saponin" was 2.3 times larger than that of control group. In view of the results so far achieved, it was revealed that red ginseng extract "saponin" has a radiation exposure protection effect in the experiment implemented at the level of cell (in vitro). In case of animal experiment, the decrement of number of red corpuscles decreased. Finally, it is necessary to carry out more various researches continuously.

• FLOWER RESEARCH JOURNAL
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• v.16 no.1
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• pp.36-43
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• 2008

This purpose of this study was to examine the effect of planting density, growing medium and strength of a nutrient solution (National Horticultural Research Institute's nutrient solution: HRI's) on the growth and development of Oriental hybrid lily 'Le Reve' in a box cultivation. The planting density with 14, 18 and 22 bulbs had sprouting one day earlier than other treatments. Planting density of 22 bulbs flowered first, while six bulbs flowered the last, indicating that higher planting densities led earlier flowering. The increasing planting density increased stem length of cut flowers. On the other hand, cut flower quality was improved when the planting density was lower. The incidence of physiological disorders such as blasting was more frequent in planting density of 22, 18, and 14, indicating that higher planting densities caused higher incidences of physiological disorders. All planting densities except 22 bulbs displayed superior results in width, weight, number, and scale weight of the bulbs. Greater planting densities led to inferior bulb enlargement and an increased decomposition rate. pH decreased in all treatments after the bulb enlargement and decreased more as the planting density increased. Contents of P, K, Ca, and Mg increased, while contents of K and Ca decreased, as the planting density increased. The rice hull+coir (1:1, v/v) treatment was better than others, but did not show that much of a difference. Moreover, in bulbs enlargement after cut flower harvest, lily medium and perlite+peat moss treatments showed superior results, and decomposition rate was the greatest in the rice hull+coir (1:1, v/v) treatment. In the HRI's solution strength treatment from the period of flower bud emergence to flower harvest, higher solution strengths gave better cut flower quality in terns of length, weight, and number of flowers. The non-treated control and one third strength of a HRI's solution hastened flowering, indicating that lower strengths led to earlier flowering. According to the results of leaf analysis as affected by solution strength during the flower harvest, absorption rates of N and K were greater when the strength was higher, and Ca and Mg showed the same tendency. On the other hand, the absorption rate of P was the lowest in all treatments.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.28 no.1
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• pp.80-98
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• 2002

Taxus cuspidata Sieb selected cultivation as drug, food and decorative plant in Kyong-gi province in Korea. As a manufacturing method, there were extracted from 250g of dried-leaf and 300g of dried-stem with each 200g of BG, PG and water (to 100) mixing for 72 hour at 50$\pm$5$\^{C}$ and then they were filtered by 400-mesh filter. Appearance of extract of leaves was slight brown, pH=5.3$\pm$0.5, gravity was 1.012$\pm$0.05, and a reflective index was 1.375$\pm$0.05. And appearance of extract of stems was slightly dark brown, pH=5.4$\pm$0.5, gravity was 1.016$\pm$0.05, and a reflective index was 1.358$\pm$0.05. It was extracted oil from Taxus seed. Gravity was 0.922$\pm$0.05 and it should be obtained the 27.0$\pm$0.5% of yield. The molecular weight of polysaccharide was about 50,000 to 300,000 dalton and contained 5.0$\pm$1.2% of yield from Taxus fruit. The determinations of total polyphenols in measuring spectropotometer got 0.563% in leaves, and 0.325% in stems, whereas the quantitives of total tannins got 0.054% and 0.037%, respectively. As the effects in Cosmetics by DPPH-method, the antioxidative activities were very strong that the inhibitory ratio showed 75% in leaves and 64% in stems compared with 52% in greentea extract. These are more effective than other plant extracts. The increasing ratio of collagen synthesis rate on the activating fibroblast for extracts of Taxus cuspidata Sieb showed 54.16% (stems) and 33.18% (leaves), To improve the skin elasticity, PPE(porcine pancreatic elastase)-inhibitory activities were strongly effective as 13,7% (stems), 23.5% (leaves) and 66%(seed). Anti-inflammatory acitvity of seed oil was very the above 41% stronger than SG was 24% of anti-Inflammatory as a control sample.

• Korean Journal of Soil Science and Fertilizer
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• v.9 no.1
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• pp.25-31
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• 1976

The purpose of this investigation is to find out the effects on the changes of microflora and the effects on the inorganic nutrients of the fertilized soil and the non-fertilized soil under submerged condition with the treatment of butachlor at the levels of 250, 500, and 1000ppm respectively. The survey was made within the period of 72 days with 7-day intervals under the incubated condition. The result came out to be the following: 1. The Fluctuated changes of soil microflora A. Bacteria: The plots treated by 500ppm of butachlor in case of non-fertilized soil showed the decreasing tendency of bacteria until 21st day. But there were no effects on the other plots. The plots treated by 250ppm and 500ppm in case of fertilized soil showed decreasing tendency on the 7th day, and 1000ppm until 21st days. B. Actinomycetes: There was no effects on the actinotnycetes in case of the non-fertilized soil but the fertilized soil showed some decrease. In both cases, actinomycetes have generally shown a little increase according to the time passage. C. Fungi: In both cases of the fertilized soil and the non-fertilized soil, the plots treated by the media showed some decreasing tendency in comparison with the control plots. There was no change to the number of fungi according to the time passage. 2. The fluctuated changes of nutrient A. $NH_4-N$: The whole plots showed decrease of $NH_4-N$ by adding butachlor. The higher the intencity of butachiar showed the more decreasing tendency of $NH_4-N$. In case of the non-fertilized soil, the highest increase of $NH_4-N$ appeared from the 7th day to 14th day, but showed degrease thereafter. The increasing tendency was seen in case of the fertilized soil plots. B. $NO_3-N$: Decrease of $NO_3-N$ was shown in the whole plots by the treatment of media, and on the 44th day of cultivation almost none of $NO_3-N$ was detected. C. $NO_2-N$: Whole plots showed the number of $NO_2-N$ highest on the 35th day, and there were nothing measurable on the 44th day. D. Eh: On the fertilized soil, the condition of reduction went on strongly but on the non-fertilized soil, the condition of reduction kept on till 42nd day and oxidation appeared thereafter.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.852-857
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• 2010

This study was performed to explore the effect of the clay mineral illite on the improvement of bed soil and plant growth. Red pepper (Capsicum annuum L.) was used as a model vegetable crop. The experiment was performed during the whole six weeks in the glass house of the Chungbuk National University. Its seedlings were cultivated in the bed soil normally used for horticultural purpose. Of the seedlings cultured, the healthy and regular size of seed were selected and cultivated in the pots. They were treated with two forms of illite, particulate (PA) and powder (PW), at the following application rates: standard application[P1 (PA1, PW1), 1:20 (w/w)], two times[P2 (PA2, PW2), 1:10 (w/w)], and four times[P4 (PA4, PW4), 1:5 (w/w)] of standard application. Untreatment (P0) was used as a control pot. At six weeks of cultivation, their growth lengths were correspondingly increased as the application rate was increased ranging from P0, P1, P2, and to P4. Their growth length was a little greater on the application of powder illite (PW) than on the particulate illite (PA). Based on the plant analysis for the root, leaf, stem of red pepper, the uptake amounts of K, Ca, and Mg, were correspondingly increased, as the application rate was increased ranging from P0, P1, P2, and to P4, respectively. At the same application rate, their amounts taken up in the respective parts were higher on the application of PW illite than on the PA one. Especially the amounts of Ca and Mg were higher in the stem, leaf than root. Consequently, it appears that the illite treatment, especially, PW form of illite, enhance the growth of red pepper in the glass house during the whole six weeks of experiment.

• Korean Journal of Remote Sensing
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• v.37 no.6_1
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• pp.1559-1572
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• 2021

The response of vegetation under the crop stress condition was evaluated using structural, biochemical, and physiological vegetation indices based on unmanned aerial vehicle (UAV) images and field-spectrometer data. A high concentration of herbicide was sprayed at the different growth stages of garlic to process crop stress, the above ground dry matter of garlic at experimental area (EA) decreased about 46.2~84.5% compared to that at control area. The structural vegetation indices clearly responded to these crop damages. Spectral reflectance at near-infrared wavelength consistently decreased at EA. Most biochemical vegetation indices reflected the crop stress conditions, but the meaning of physiological vegetation indices is not clear due to the effect of vinyl mulching. The difference of the decreasing ratio of vegetation indices after the herbicide spray was 2.3% averagely in the case of structural vegetation indices and 1.3~4.1% in the case of normalization-based vegetation indices. These results meant that appropriate vegetation indices should be utilized depending on the types of crop stress and the cultivation environment and the normalization-based vegetation indices measured from the different spatial scale has the minimized difference.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.2
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• pp.63-69
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• 2019

Black-colored rice contains anthocyanin, which has an antioxidant function on the seed coat. Anthocyanin content is greatly affected by the cultivation environment, especially the average temperature during the ripening stage. Generally, low temperatures during the ripening stage increase anthocyanin content. To control the average temperature during ripening stage in the field, transplanting time has to be regulated. In this study, anthocyanin content variation was examined in relation to the transplanting time and the average temperature during the ripening stage. For the study, fourteen black-colored rice cultivars with different maturity types (four of early-maturing, five of medium-maturing, and five of medium-late maturing) were selected. The transplanting times used were May 20, June 5, June 20, and June 30. The field experiment was conducted in the Miryang, Kyoungsangnamdo province, Korea from 2014 to 2017. The anthocyanin content in all cultivars was higher when the transplanting time was delayed, and the highest anthocyanin content was observed in the transplanting on June 30. Variation in anthocyanin content according to the change in transplanting time is the greatest in the early maturing cultivars. The least change was observed in medium maturing cultivars. Regression analysis showed a significant correlation between temperature and anthocyanin content, but the degree of correlation was very low in the medium maturing cultivar. As a result, the optimal average temperature during the grain filling stage for increasing the anthocyanin content of black colored rice was $22{\sim}23^{\circ}C$. The rice yield increased in plants transplanted until June 20 and decreased thereafter owing to low temperature during the grain filling stage. The anthocyanin content increased with delaying the transplanting time up to June 30 but the rice yield decreased after June 20. Nevertheless, the rate of increase in anthocyanin content was higher than the rate of decrease in rice yield. As a result, the optimum transplanting time and an average temperature of grain filling stage for black-colored rice variety were June 30 and $23{\sim}24^{\circ}C$ considering both anthocyanin content and rice yield.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.287-296
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• 2021

Recently, the pace of global climate change has tremendously increased, causing extreme damage to crop production. Here, we aimed to examine the growth characteristics and useful components of Angelica gigas under extreme heat stress, providing fundamental data for its efficient cultivation. Plants were exposed to various experimental temperatures (28℃, 34℃, and 40℃), and their growth characteristics and content of useful components were analyzed. At the experimental site, the ambient and soil temperature were 19.38℃ and 21.34℃, ambient and soil humidity were 81.3 % and 0.18 m³/m³, solar radiation was 162.05 W/m². Moreover, the soil was sandy-clay-loam (pH 6.65), with 2.66% organic matter, 868.52 mg/kg soil available phosphate, and 0.14% nitrogen. Values of most growth characteristics, including the survival rate (85%), plant height (38.66cm), and fresh and dry weight (41.3 g and 14.24 g), were the highest at 28℃. Although the highest content of useful components was observed at 34℃ (3.24%), there were no significant differences across temperatures. Growth characteristics varied across temperatures due to detrimental effects of heat stress, such as accelerated tissue aging, reduced photosynthesis, and delay of growth. Similar content of useful components across temperatures may be due to poor accumulation of anabolic products caused by impaired growth at extremely high temperatures.