• Title/Summary/Keyword: Crystal Orientation

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Use of Molecular Replacement to Determine the Phases of Crystal Structure of Taq DNA Polymerase

  • Kim, Young-Soo;Suh, Se-Won
    • BMB Reports
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    • v.29 no.1
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    • pp.38-44
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    • 1996
  • Taq DNA polymerase from Thermus aquaticus has been shown to be very useful in the polymerase chain reaction method, which is being used for amplifying DNA. Not only does Taq DNA polymerase have high commercial value commercial value for the polymerase chain reaction application, but it is also important in studying DNA replication, because it is apparently an homologue to E. coli DNA polymerase I, which has long been used for DNA replication study (Lawyer et ai., 1993). The crystal structure determination of Taq DNA polymerase was initiated. An X-ray diffraction pattern breaks down a crystal structure into discrete sine waves in a Fourier series. The original shape of a crystal object in terms of electron density may be represented as the sum of those sine waves with varying amplitudes and phases in three dimensions. The molecular replacement method was initially employed to provide phase information for the structure of Taq DNA polymerase. The rotation search using the program MERLOT resulted in a solution peak with 5.4 r.m.s. PC-refinement of the X-PLOR program verified the result and also optimized the orientation angles. Next, the translation search using the X-PLOR program resulted in a unique solution peak with 7.35 r.m.s. In addition, the translation search indicated $P3_121$ to be the true space group out of two possible ones. The phase information from the molecular replacement was useful in the MIR phasing experiment.

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Transverse Wave Propagation in [ab0] Direction of Silicon Single Crystal

  • Yun, Sangjin;Kim, Hye-Jeong;Kwon, Seho;Kim, Young H.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.35 no.6
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    • pp.381-388
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    • 2015
  • The speed and oscillation directions of elastic waves propagating in the [ab0] direction of a silicon single crystal were obtained by solving Christoffel's equation. It was found that the quasi waves propagate in the off-principal axis, and hence, the directions of the phase and group velocities are not the same. The maximum deviation of the two directions was $7.2^{\circ}$. Two modes of the pure transverse waves propagate in the [110] direction with different speeds, and hence, two peaks were observed in the pulse echo signal. The amplitude ratio of the two peaks was dependent on the initial oscillating direction of the incident wave. The pure and quasi-transverse waves propagate in the [210] direction, and the oscillation directions of these waves are perpendicular to each other. The skewing angle of the quasi wave was calculated as $7.14^{\circ}$, and it was measured as $9.76^{\circ}$. The amplitude decomposition in the [210] direction was similar to that in the [110] direction, since the oscillation directions of these waves are perpendicular to each other. These results offer useful information in measuring the crystal orientation of the silicon single crystal.

Prediction of Deformation Texture in BCC Metals based on Rate-dependent Crystal Plasticity Finite Element Analysis (속도의존성 결정소성 모델 기반의 유한요소해석을 통한 BCC 금속의 변형 집합조직 예측)

  • Kim, D.K.;Kim, J.M.;Park, W.W.;Im, Y.T.;Lee, Y.S.
    • Transactions of Materials Processing
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    • v.23 no.4
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    • pp.231-237
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    • 2014
  • In the current study, a rate-dependent crystal plasticity finite element method (CPFEM) was used to simulate flow stress behavior and texture evolution of a body-centered cubic (BCC) crystalline material during plastic deformation at room temperature. To account for crystallographic slip and rotation, a rate-dependent crystal constitutive law with a hardening model was incorporated into an in-house finite element program, CAMPform3D. Microstructural heterogeneity and anisotropy were handled by assigning a crystallographic orientation to each integration point of the element and determining the stiffness matrix of the individual crystal. Uniaxial tensile tests of single crystals with different crystallographic orientations were simulated to determine the material parameters in the hardening model. The texture evolution during four different deformation modes - uniaxial tension, uniaxial compression, channel die compression, and simple shear deformation - was investigated based on the comparison with experimental data available in the literature.

Luminescent Properties of BaSi2O5:Eu2+ Phosphor Film Fabricated by Spin-Coating of Ba-Eu Precursor on SiO2 Glass

  • Park, Je Hong;Kim, Jong Su;Kim, Jong Tae
    • Journal of the Optical Society of Korea
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    • v.18 no.1
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    • pp.45-49
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    • 2014
  • Well-crystallized $BaSi_2O_5:Eu^{2+}$ phosphor films were synthesized by heat treatment of spin-coated BaO:Eu on $SiO_2$ glass. We investigated luminescence-structure properties of these phosphor films as a function of heat-treatment temperature. From x-ray diffraction patterns, our $BaSi_2O_5:Eu^{2+}$ phosphor films revealed that (111)- and (204)-crystal planes of $BaSi_2O_5$ crystal were dominantly increased with an increase of heat-treatment temperature. Photoluminescence intensities of $BaSi_2O_5:Eu^{2+}$ phosphor films were increased with amount of these crystal planes. It can be explained that $Eu^{2+}$ ions were stably occupied at specific crystal orientation of $BaSi_2O_5$ crystal, enhancing the luminescent intensities of $BaSi_2O_5:Eu^{2+}$ phosphor films. In addition, our $BaSi_2O_5:Eu^{2+}$ phosphor films had transmittance of 70% at 510 nm,.due to the dense morphology and specific crystallinity of $BaSi_2O_5:Eu^{2+}$ phosphor films.

The growth YMnO$_3$ single crystals using a floating zone method (부유대용융법에 의한 YMnO$_3$단결정 성장)

  • 권달회;강승구;김응수;김유택;심광보
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.10 no.4
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    • pp.279-285
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    • 2000
  • High quality crystals of $YMnO_3$, which is interested in non-volatile memory device application, were grown by the floating zone method. Optimum condition for powder synthesis was established to be $1200^{\circ}C$ for 10 hrs and optimum condition for sintering of $YMnO_3$feed-rod was established to be $1500^{\circ}C$ for 10hrs respectively. It was found from non-seeded growth experiment that $YMnO_3$crystal was grown preferentially to the [1010] orientation. The $YMnO_3$single crystal, which was grown to the direction of perpendicular to C-axis, was typically 5mm in diameter, 50 mm in length and showed dark-blue color.

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Thermal behavior of the layered structure of decanesulfonate intercalated into the hydrated nickel compound (데칸술폰이 삽입된 니켈 화합물의 층상 구조의 열적 성질)

  • 허영덕;전태현;박용준
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.9 no.6
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    • pp.580-584
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    • 1999
  • The synthesis and characterization of intercalated compound of decansulfonate into hydrated nickel is presented. The compound shows a layered structure as determined by high temperature powder X-ray diffraction (HTXRD). The layer distance of the product is increased from 24.7 $\AA$ to 30.5 $\AA$ by increasing the temperature which is in turn accomplished by changing the structure of the intercalated nickel compound. From the X-ray diffraction data and the decanesulfonate size, the orientation of the decanesulfonate onto the nickel layer is determined. The molecular axis of the decanesulfonate with bilayer structure is tilted to the perpendicular of the nickel layer.

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The structure and synthesis of intercalation compounds between layered basic copper acetate and alkyl sulfonates (알킬술폰이 삽입된 층상구조의 염기성 초산구리의 합성과 구조)

  • 조영식;허영덕
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.8 no.3
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    • pp.431-434
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    • 1998
  • Layered basic copper acetate, $Cu(OH)(CH_3COO){cdot}H_2O$ was synthesized. Intercalation compounds of alkyl sulfonates into layered basic copper acetate have been synthesized via anionic exchange. From the X-ray diffraction data and the alkyl sulfonate size, the orientation of the intercalated alkyl sulfonate into layered basic copper acetate was determined.

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Optically Detected Magnetic Resonance with Nitrogen-Vacancy Spin Ensemble in Diamond

  • Lee, Hyun Joon;Shim, Jeong Hyun
    • Journal of the Korean Magnetic Resonance Society
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    • v.22 no.2
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    • pp.40-45
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    • 2018
  • We report Optically-Detected Magnetic Resonance (ODMR) study on Nitrogen-Vacancy (NV) centers in diamond. The experiment can easily be conducted with basic optics and microwave components. A diamond crystal having a high-density NV center is suitable for the ODMR study. The magnetic field dependence of ODMR spectrum allowed us to determine the orientation of the diamond crystal. In addition, we measured the variation of the ODMR spectrum as a function of the excitation laser power. Thermal heating induced by optical absorption caused the monotonic decrease of zero field splitting. The contrast of the ODMR peak, however, increased and, then, began to decrease, indicating the optimal laser power for recording the ODMR spectrum.

Effect of Electric field on an Injection Velocity in a Vertically Aligned Nematic Liquid Crystal (수직배향 네마틱 액정셀에서의 주입속도에 미치는 전기장 효과)

  • Jeon, Yeon-Mun;Kim, Sang-Gyun;Kim, Youn-Sik;An, Myeong-Hwan;Lee, Seung-Hee
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.19 no.7
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    • pp.695-699
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    • 2006
  • Injection time of liquid crystal (LC) by capillary action in a vertically aligned (VA) nematic LC cell takes longer than that in a homogeneously aligned (HA) LC cell because Miesowicz viscosity in the former is bigger than that in the latter. To reduce liquid crystal injection time in the VA cell, we applied vertical electric field while injecting so that the orientation of LC molecules is changed from vertical alignment to homogeneous alignment. Consequently, the injection speed is improved by 25 % when compared with the cell without an applied field.