• 생약학회지
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• 제16권1호
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• pp.18-25
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• 1985

Experimental studies were conducted to investigate for the effect of Tongkyu-tang on analgesic, antipyretic, sedative, anti-inflammatory, isolated ileum and anti-histamine actions. Tongkyu-Tang was composed of sixteen crude drugs including Astragali Radix, which has been used for the treatment of common cold, headache, rhinitis, nasal obstruction, rhincospasm, etc. The following results have been obtained; analgesic and antipyretic actions were obtained. Prolonged action against the hypnotic duration of pentobarbital were significantly noted in mice. Anti-inflammatory effects in the paw edema induced by 1% carrageenin and 1% dextran were significantly shown in rats. Spontaneous motilities of isolated ileum of mice were strongly suppressed, and contractions of isolated ileum of mice and guinea-pigs induced by acetylcholine chloride, barium chloride and histamine were remarkably inhibited. Antihistamine actions were noted. Considering the above experimental results, it is suggested that effects of Tongkyu-tang based on the Oriental Medical References were similarly consistent with the actual experimental results.

• 생약학회지
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• 제13권3호
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• pp.122-128
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• 1982

In order to investigate the pharmacological action of combined preparation of crude drugs, 'Oyo-Tang' were studied. 'Oyo-Tang' consist of Glycyrrhizae Radix, Ephedrae Herba, Armenicae Semen, Platycodi Radix and Nepetae Herba. They have widely been prescribed in Oriental Medicine for controlling of cough, asthma, nasal abstraction, pharygeal pain and hoarseness. Experimental studies were implemented on analgesic, sedativc, anticonvulsive and ileum relaxing actions. The results of the studies were as follows; Significant anticonvulsive action was recognized by fraction IIII. Sedative and analgesic actions were noted by fraction I and III. Relaxing action was shown on the extracted ileum in mice, antagonistic actions were seen on $BaCl_2-induced$ contraction of the ileum of mice and Histamine-induced contraction of the ileum of guinea-pigs that the relaxing effect of the intestinal smooth muscle was recognized.

• 생약학회지
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• 제16권2호
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• pp.73-80
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• 1985

Experimental studies were undertaken to investigate for the effect of Yijin-tang on the digestive system and isolated uterus of rats. Yijin-tang was composed of five crude drugs; Pinelliae Tuber, Aurantii nobilis Pericarpium, Hoelen, Glycyrrhizae Radix and Zingiberis Rhizoma. The results obtained from experiments with water soluble fraction extracted from Yijin-tang were as follows; Spontaneous motilities of isolated ileum of mice and rabbits were suppressed, and contraction of isolated ileum of mice and guinea-pigs induced acetylcholine, barium chloride and histamine were inhibited. The anticathartic action of mice induced by castor oil were significantly observed. The spontaneous motility of isolated uterus of rats was suppressed. Inhibitory effect of gastric juice secretion and antiulcerogenic action were significantly shown in Shay rats.

• Natural Product Sciences
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• 제17권2호
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• pp.113-116
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• 2011

Arginase competitively inhibits nitric oxide synthase (NOS) via use of the common substrate L-arginine. Arginase II has recently reported as a novel therapeutic target for the treatment of cardiovascular diseases such as atherosclerosis. In our experiment, the EtOH extracts of four-hundreds extracts drugs were investigated for the arginase inhibitory activity. Among them, four extracts exhibited over 50% inhibition of arginase II activity compared to control at a concentration of 150${\mu}g/ml$. In particular, the seed of Arctium lappa, gum-resin of Boswellia carterii, aerial part of Artemisia apiacea and rhizome of Cyperus rotundus inhibited arginase II activity, with $IC_{50}$ values of 118.4, 135.4, 123.9 and 86.7${\mu}g/ml$, respectively. In addition, four plant extracts showed less than 20% inhibition of arginase I activity at 150${\mu}g/ml$. These plants might be the potential candidate materials in the development of the novel atherosclerosis drug.

• 대한수의학회지
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• 제2권2호
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• pp.15-24
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• 1962

In the previous report it was demonstrated that aqueous extracts of two Korean indigenous crude drugs, Radix Jingyu and Fructus ponciri, have an excellent tick repellent effect, and also the need for further analytical study of these crude drugs was suggested. In the experiments to be reported herein, attempts were made to find out the active ingredients from Radix Jingyu, since the aqueons extract of Radix Jingyu proved to be mone effective than that of Fructus ponciri. It will be shown that the active ingredient is indicative of Lycaconitine, a known alkaloid. An aqueons solution containing 1.5 Gms of Lycaconitine in tartrate when sprayed on a cow exerted tick repellent effect against Boophilus microplus which lasted approximately 25 days without any harmful influences on cattle. It will also be shown that the oil-fat components of Radix Jingyu act synergistically with Lycaconitine in accelerating and lengthening the effect, although the components alone have no effect under the conditions tested. It was of interest to note that the oil-fat components of Radix Jingyu showed definite repellent effect against the larvae of Boophilus microplus in laboratory experiments by the method newly devised. On the basis of these observations, it was concluded that Lycaconitine can be used as cattle tick repellent, and the evidence seems to warrant a large scale production of Lycaconitine for its widespread use. Further studies on the possible effect of the alkaloid on other genera or species of ticks and related vermins were also indicated.

• 대한한의학회지
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• 제20권4호
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• pp.11-15
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• 2000

Radix Angelicae Gigantis is sweet and pungent in flavor, warm in property. Its effects are tonifying the blood, promoting blood circulation, relieving pain and moistening the bowels. Its indications are blood deficiency syndrome characterized by sallow complexion, dizziness, irregular menstruation, amenorrhea, pains due to blood stasis, and rheumatic arthralgia. Using genes of A. gigas, A. acutiloba, and A. sinensis, the origin of which is identified, as criteria, we analysed many kinds of Angelica with RAPD and RFLP on ITS region, in order to compare and discriminate genes extracted from crude drugs ‘Dang-gui’, that are produced in Korea on the one hand and imported on the other hand. We reached the following conclusion. 1. We could extract DNA from both original plant and dried plant. 2. Especially Uniprimer #1, Uniprimer #2, Uniprimer #4 and Uniprimer ＃9 were useful. 3. Among the restriction enzymes Sma I, Msp I, Hae III, and Hinf I, used in this experiment, four restriction enzymes except Hinf I could be used properly in discriminating all samples used as A. gigas. We think that this result can be used as a method of discriminating crude drug of Angelica L. related drugs, and used in controlling quality and circulation.

• 생약학회지
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• 제13권1호
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• pp.33-38
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• 1982

In order to investigate the pharmacological action of the combined preparation of crude drugs, 'Saengkankunbi-Tang' were studied. These studies were conducted in an attempt to investigate the effect of 'Saengkankunbi-Tang' on activities of the liver enzyme, protein contents and the excretory action of bile juice in the serum of $CCl_4-intoxicated$ rabbits. The results of this investigate were summarized as follows; GOT, GPT activities and total cholesterol contents in the serum of rabbits induced by $CCl_4$ were shown to the remarkable decrease with the passage of the time in the oral administration of the sample. Albumin contents in the serum of rabbits induce by $CCl_4$ were noted to the significant increase with the passage of the time. Serum total protein contents were noted to the significant increase only the 4th and 6th day. BSP accumulation contents in the serum of rabbits induced by $CCl_4$ showed the remarkable decrease with the passage of the time.

• 생약학회지
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• 제40권4호
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• pp.370-375
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• 2009

Overproduction of peroxynitrite ($ONOO^-$) causes a variety of disease such as atherosclerosis, hypercholesterolemia, diabetes mellitus or obesity. Peroxynitrite scavenging activities and HPLC analysis on the five Oriental medicinal drugs belonging to the genus Citrus, Aurantium or Poncirus (Rutaceae family) and HPLC analysis were taken to evaluate flavanone glycosides with peroxynitrite scavenging activity. The $IC_{50}s$ of the five crude drugs were shown as follows: Aurantii nobilis Pericarpium (Jinpi, 18.3 ${\mu}g$/ml), Citrii unshiu Pericarpium (Chungpi, 7.50${\mu}g$/ml), Citrii unshiu Semen (Gyulhaek, >50.0${\mu}g$/ml), Aurantii Fructus (Jigak, 18.3${\mu}g$/ml), and Poncirii Fructus (Jisil, >50.0${\mu}g$/ml) where Korean crude drug's names are noted in the parenthesis. Peroxynitrite scavenging effect of flavanones or their glycosides usually contained in Citrus species were observed as follows: hesperetin (1.89 ${\mu}g$/ml), naringenin (7.77 ${\mu}g$/ml), hesperidin (8.44 ${\mu}g$/ml), poncirin (>50.0 ${\mu}g$/ml)and ponciretin(>50.0 ${\mu}g$/ml). The activities of naringin and poncirin with ${\alpha}$-L-rhamnopyranosyl($1{\rightarrow}2$)-${\beta}$-D-glucopyranosyl moiety were weak. HPLC analytical data revealed that Jinpi (the peels of mature fruits of Citrus unshiu) and Chungpi (the peels of immature fruits of C. unshiu) had high quantities of hesperidin as the value of 142.1${\pm}$0.21 and 104.51${\pm}$1.10 mg/g dried weight, respectively. Poncirin was clearly detected in only Jisil and naringenin and naringin were not observed on the HPLC chromatogram of the five crude drugs.

• 약학회지
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• 제35권3호
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• pp.203-215
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• 1991

Using the calorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT)assay, we evaluated the chemosensitivity of 8 anticancer drugs{vincristine(VCR), vinblastine(VBL), adriamycin(ADR), cisplatin(CPDD), etoposide(VP-16), cytosine arabinoside(ara-C), bleomycin (Bleo) and cyclophosphamide(CYC)} and the cytotoxicity-enhancing effects of ($\pm$)-ar-turmerone and the extracts of the crude drugs {Lithospermum eythrorhizon(LE) and Scutellaria baicalensis (SB)} on the above mentioned anticancer drugs against HL-60 and KG-1 cells among 8 anticancer drugs, VCR, VBL, ADR, and CPDD inhibited the growth of both cell lines by more than 50%, while VP-16, ara-C, Bleo, and CYC were less effective. ($\pm$)-ar-Turmerone had significant inhibitory effects against both cell lines, showing the ID$_{50}$ values of 11.730 $\mu\textrm{g}$/ml and 0.292 $\mu\textrm{g}$/ml for HL-60 and KG-1 cells. respectively. But the extracts of LE and SB roots showed no significant cytotoxic effects. According to ID$_{50}$ values, the cytotoxicities of VCR, VBL and ADR against HL-60 were enhanced two, eight and three times by mixing ($\pm$)-ar-turmerone, five, seven and three times by adding the extract of LE root, and twenty, six and three times by mixing the extract of SB root, respectively. The cytotoxicities of the above mentioned drugs against KG-1 cell were enhanced two, seven and three times by mixing ($\pm$)-ar-turmerone, two, three and three times by combining wilth the extract of LB root, and two, five and two times by adding the extract of SB root, respectively. The cytotoxicity-potentiating effects of ($\pm$)-ar-turmerone and the extracts of LE and SB roots against HL-60 cell were greater than KG-1 cell.

• 혜화의학회지
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• 제5권1호
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• pp.185-198
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• 1996

After evaluation of antithrombotic effect of 40 herbs on platelet aggregation induced by ADP(Adenosine diphosphate), these results were obtained as follows: 1. Crude drugs exerting over 30 % of in Chinemys reevesii (Gray)hibition on platelet aggregation induced by ADP were Ganoderma japonicum (Fr.) Lloyd., Panax ginseng C. A. Mey., Gastrodia elata Bl., Thea sinensis, Chinemys reevesii (Gray), Cuscuta chinensis Lam., Cervus nippon Temminck., Biota orientalis (L.) Endl., Coriolus versicolor, Cinnamomum cassia Presl., Sophora flavescens Ait., Amomum villosum Lour., Carthamus tinctorius L., Rubus chingii Hu., Poria cocos (Schw.) Wolf., Laminana japonica Aresch., Ligustrum lucidum Ait., Angelica sineusis (Oliv.), Cyperus rotundas L., Ginkgo biloba L., Zingiber officinale Rosc., Prunus persica (L.) Batsch., Schizandra chinensis (Turcz.) Baill. and Plantago asiatica L.. 2. Of crude drugs having showed over 50% of inhibitory effect on platelet aggregation, at the concentration of $100{\mu}g/m{\ell}$, the inhibitory rates were 82.2% in Ganoderma japonicum (Fr.) Lloyd., 55% in Panax ginseng C. A. Mey., 50.8% in Gastrodia elata Bl., while at the concentration of $200{\mu}g/m{\ell}$, antithrombotic rates were 89.4% in Ganoderma japonicum (Fr.) Lloyd., 59.2% in Panax ginseng C. A. Mey., 57.9% in Thea sinensis, 52.7% in Gastrodia elata Bl.. These results suggest that the study sholuld be necessary on antithrombotic effect of solvent fractions of Ganoderma japonicum (Fr.) Lloyd., Panax ginseng C. A. Mey., Gastrodia elaha B1. and Thea sinensis and isolation of effective compound from above drugs.