• 생약학회지
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• 제11권3_4호통권43호
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• pp.133-140
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• 1980

In order to develop the radio-immunoassay procedure for the ginsenoside $Rg_1$ we prepared the $Rg_1-BSA$ conjugate and $Rg_1-tyramine$ conjugate by condensing the $Rg_1-azide$, which was prepared by a series of six step chemical modification of the $Rg_1-side$ chain, with bovine serum albumin(BSA) or with tyramine. Rabbits were immunized by repeated injection of $Rg_1-BSA$ conjugate with Freund's Complete Adjuvant for 5 month long to obtain very potent $anti-Rg_1$ serum. The radio-labelled haptene was prepared by direct radio-iodination $(125_J)$ of $Rg_1-tyramine$ according to the chloramine-T method. The radio-immunoassay procedure was successfully furnished by using DCC method (dextran coated charcoal) and the anti-body titer of the anti-serum was found as being $1600{\sim}3200$ by using 15000cpm tracer per test. Calibration test using non-labelled $Rg_1$ showed linear competetive binding response in the $(8-300){\times}34pg$. range of non-labelled $Rg_1$. The cross reaction test using 19 ginsenoside analogues enabled us a full structure-activity analysis on the antigen-antibody reaction that the anti-body in the serum would recognize the full structure of ginsenoside $Rg_1$ except the side chain moiety.

• 한국해양공학회지
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• 제29권2호
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• pp.169-174
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• 2015

This paper addresses experimental methodologies to measure the temperature-dependent thermal conductivity of the insulation materials popularly used for LNG cargo containment systems. The measurement techniques considered in this paper are the guarded hot plate (GHP) method and heat flow method (HFM). The former is based on the power supplied to the hot plate to keep the temperature constant, and the latter is based on a direct heat flux measurement. In order to improve the accuracy of the HFM, the thermal conductivity obtained by GHP was cross-compared with the HFM results, and a calibration factor was derived. It was found that the thermal conductivities measured by the two methods corresponded well under room temperature, but the deviation tended to slightly increase as the temperature decreased. Because of the easy installation and operability of HFM, it can be used to measure thermal conductivity in a large scale mock-up test or unit insulation panel test, where the GHP method is difficult to apply.

• 전기학회논문지
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• 제62권6호
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• pp.833-839
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• 2013

Cardiopulmonary resuscitation (CPR) is performed by thoracic compression and artificial ventilation for the patient under emergent situation to maintain at least the minimum level of respiration and blood circulation for life survival. Good quality CPR requires monitoring respiration, however, traditional respiratory air flow transducers cannot be used because the transducer elements are facing the whole area perpendicular to the flow axis. The present study developed a new air flow transducer conveniently applicable to CPR. Specially designed "sensing rod" samples the air velocity at 3 different locations of the flow cross-section, then transforms into average dynamic pressure by the Bernoulli's law. The symmetric structure of the sensing holes of the sensing rod enables bi-directional measurement simply by taking the difference in pressure by a commercial differential pressure transducer. Both inspiratory and expiratory flows were obtained with symmetric measurement characteristics. Quadratic curve fitting provided excellent calibration formula with a correlation coefficient>0.999 (P<0.0001) and the mean relative error<1%. The present results can be usefully applied to accurately monitor the air flow rate during CPR.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 춘계학술대회
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• pp.1951-1956
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• 2004

The transient nature and complex flow geometries of two-phase gas-liquid flows cause fundamental difficulties when measuring flow velocity using an electromagnetic flowmeter. Recently, a current-sensing flowmeter was introduced to obtain measurements with high temporal resolution (Ahn et $al.^{(1)}$). In this study, current-sensing flowmeter theory was applied to measure the fast velocity transients in slug flows. To do this, the velocity fields of axisymmetric gas-liquid slug flow in a vertical pipe were obtained using Volume-of-Fluid (VOF) method and the virtual potential distributions for the electrodes of finite size were also computed using the finite volume method for the simulated slug flow. The output signal prediction for slug flow was carried out from the velocity and virtual potential (or weight function) fields. The flowmeter was numerically calibrated to obtain the cross-sectional liquid mean velocity at an electrode plane from the predicted output signal. Two calibration parameters are required for this procedure: a flow pattern coefficient and a localization parameter. The flow pattern coefficient was defined by the ratio of the liquid resistance between the electrodes for two-phase flow with respect to that for single-phase flow, and the localization parameter was introduced to avoid errors in the flowmeter readings caused by liquid acceleration or deceleration around the electrodes. These parameters were also calculated from the computed velocity and virtual potential fields. The results can be used to obtain the liquid mean velocity from the slug flow signal measured by a current-sensing flowmeter.

• 한국수자원학회논문집
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• 제43권3호
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• pp.283-294
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• 2010

본 연구의 목적은 저유량에서 발산하는 문제를 해결하여 다양한 흐름과 불규칙한 하도단면을 반영하는 안정적이고 정교한 동적수질해석모형을 개발하는데 있다. 이에 본 연구에서는 기존 모형들의 저유량에서 발산하는 문제를 해결하고자 확산파 운동방정식 기법을 이용하여 수리해석모형인 DyHYD를 개발하였으며, 수질해석모형은 자생 BOD와 수온, DO, TN, TP 등 12가지 수질농도를 계산할 수 있는 DyQUAL을 개발하였다. 개발된 모형의 적용성을 검토하기 위하여 가상하도 및 실제 낙동강유역에서 모의를 수행한 후 실측치와 비교 검토함으로써 모형의 적용성과 신뢰성을 검토하였다. 모형의 수행결과를 관측치와의 비교를 통해 Nash-Sutcliffe 계수를 산정하였으며, 보정과정에서는 유량과 BOD, TN, TP의 Nash-Sutcliffe 계수는 0.391에서 0.591의 범위를 나타내며, 검증과정에서는 0.704에서 0.902의 범위를 나타내었다.

• 한국전자파학회논문지
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• 제29권2호
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• pp.118-127
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• 2018

본 논문에서는 합성개구레이다(Synthetic Aperture Radar: SAR) 장비의 성능 분석을 위하여 레이다 표적모의장치를 설계, 제작한 결과를 선보인다. 먼저 표적모의장치의 기능과 성능에 대해 설명하고, SAR 장비와 연동하기 위한 표적 시나리오 생성방법에 대해 설명하였다. 또한 제작된 표적모의장치를 이용하여 SAR 장비의 시간지연 값을 간단하고 정확하게 측정하고 보상하는 방법을 개발하였다. 시간지연이 보상된 SAR 장비와 표적모의장치를 이용하여 SAR 영상에서 점표적을 획득하고, IRF(Impulse Response Function) 분석을 통해 성능을 분석하였다. 그 결과, 방위방향 IRF의 PSLR(Peak to Side Lobe Ratio)은 -13.25 dB, 해상도는 0.49 m로 이론값과 매우 유사한 값을 보였다.

• 한국작물학회지
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• 제57권1호
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• pp.29-34
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• 2012

The objective of this study was to rapidly evaluate fatty acids in a collection of millet (Panicum miliaceum subsp. miliaceum) of different origins so that this information could be disseminated to breeders to advance germplasm use and breeding. To develop the calibration equations for rapid and nondestructive evaluation of fatty acid content, near-infrared reflectance spectroscopy (NIRs) spectra (1104-2494 nm) of samples ground into flour ($n$=100) were obtained using a dispersive spectrometer. A modified partial least-squares model was developed to predict each component. For foxtail millet germplasm, our models returned coefficients of determination ($R^2$) of 0.89, 0.89, 0.89, and 0.92 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively. The prediction of the external validation set (n=10) showed significant correlation between references values and NIRs values ($r^2$=0.64, 0.90, 0.79, and 0.89 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively). Standard deviation/standard errors of cross-validation (SD/SECV) values were close to 3 (2.62, 2.40, 1.85, and 2.23 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively). These results indicate that these NIRs equations are functional for the mass screening and rapid quantification of the oleic and total fatty acids characterizing millet germplasm. Among the samples, IT153514 showed an especially high content of fatty acids ($48.14mg\;g^{-1}$), whereas IT123909 had a very low content ($34.44mg\;g^{-1}$).

• 한국지리정보학회지
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• 제12권3호
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• pp.21-34
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• 2009

홍수 시 흐름은 기본적으로 2차원 흐름으로서 이 때 하천 흐름의 수위와 유속 분포는 1차원 하천 모형으로 적절한 결과를 도출하기 어렵다. 이에 본 연구에서는 GIS를 이용하여 낙동강의 합류점에 대한 2차원 유한요소망을 구축하고 RMA-2모형을 이용하여 모의를 수행하였다. 이를 위해 모형의 매개변수들이 해석결과에 미치는 영향을 검토하였으며, 모형의 적용성을 검토하기 위해 금호강 합류부에 대해 흐름해석을 실시하고 검증하였다. 또한 하천의 단면 변화에 따른 흐름변화를 판단하기 위하여 과거 단면과 최근 단면에 대해 모의를 실시하고 비교 검토하였다. 그 결과 합류부에 대한 2차원 모형의 적용성을 검증하고 단변변화의 영향이 수위에 미치는 영향을 확인하여, 향후 합류부 처리, 하구부 처리, 제방축조 등에 효율적으로 활용될 수 있을 것으로 판단된다.

• 한국육종학회지
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• 제42권5호
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• pp.448-454
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• 2010

The objective of this study was to rapidly evaluate fatty acids in a collection of foxtail millet (Setaria italica (L.) P. Beauv) of different origins so that this information could be disseminated to breeders to advance germplasm use and breeding. To develop the calibration equations for rapid and nondestructive evaluation of fatty acid content, near-infrared reflectance spectroscopy (NIRs) spectra (1104-2494 nm) of samples ground into flour (n=100) were obtained using a dispersive spectrometer. A modified partial least-squares model was developed to predict each component. For foxtail millet germplasm, our models returned coefficients of determination ($R^2$) of 0.91, 0.89, 0.98 and 0.98 for strearic acid, oleic acid, linoleic acid, and total fatty acids, respectively. The prediction of the external validation set (n=10) showed significant correlation between references values and NIRs values ($r^2=0.97$, 0.91, 0.99 for oleic, linoleic, and total fatty acids, respectively). Standard deviation/standard error of cross-validation (SD/SECV) values were greater than 3 (3.11, 5.45, and 7.50 for oleic, linoleic, and total fatty acids, respectively). These results indicate that these NIRs equations are functional for the mass screening and rapid quantification of the oleic, linolenic, and total fatty acids characterizing foxtail millet germplasm. Among the samples, IT153491 showed an especially high content of fatty acids ($84.06mg\;g^{-1}$), whereas IT188096 had a very low content ($29.92mg\;g^{-1}$).

• 대한수의학회지
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• 제60권4호
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• pp.209-213
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• 2020

This study examined the residue of dl-methylephedrine hydrochloride (MEP) on the muscle of pigs administered orally with MEP 12 g/ton feed for seven consecutive days. Twenty healthy cross swine were administered MEP. Four treated animals were selected arbitrarily to be sacrificed at 1, 2, 3, 4, and 5 days after treatment. MEP residue concentrations in the muscle were determined by liquid chromatography coupled with tandem mass spectrometry. The drug was extracted from muscle samples using 10 mM ammonium formate in acetonitrile followed by clean-up with n-hexane. The analyte was separated on an XBridgeTM hydrophilic interaction liquid chromatography column using 10 mM ammonium formate in deionized distilled water and acetonitrile. The correlation coefficient (R2) of the calibration curve was 0.9974, and the limits of detection and quantification were 0.05 and 0.15 ㎍/kg, respectively. The recoveries at three spiking levels were 94.5-101.2%, and the relative Standard Deviations was less than 4.06%. In the MEP-treated group, MEP residues on one day post-treatment were below the maximum residue limit in the muscle. The developed method is sensitive and reliable for the detection of MEP in porcine muscle tissues. Furthermore, it exhibits low quantification limits for animal-derived food products destined for human consumption.