• Korean Journal of Pharmacognosy
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• v.11 no.3_4 s.43
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• pp.133-140
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• 1980

In order to develop the radio-immunoassay procedure for the ginsenoside $Rg_1$ we prepared the $Rg_1-BSA$ conjugate and $Rg_1-tyramine$ conjugate by condensing the $Rg_1-azide$, which was prepared by a series of six step chemical modification of the $Rg_1-side$ chain, with bovine serum albumin(BSA) or with tyramine. Rabbits were immunized by repeated injection of $Rg_1-BSA$ conjugate with Freund's Complete Adjuvant for 5 month long to obtain very potent $anti-Rg_1$ serum. The radio-labelled haptene was prepared by direct radio-iodination $(125_J)$ of $Rg_1-tyramine$ according to the chloramine-T method. The radio-immunoassay procedure was successfully furnished by using DCC method (dextran coated charcoal) and the anti-body titer of the anti-serum was found as being $1600{\sim}3200$ by using 15000cpm tracer per test. Calibration test using non-labelled $Rg_1$ showed linear competetive binding response in the $(8-300){\times}34pg$. range of non-labelled $Rg_1$. The cross reaction test using 19 ginsenoside analogues enabled us a full structure-activity analysis on the antigen-antibody reaction that the anti-body in the serum would recognize the full structure of ginsenoside $Rg_1$ except the side chain moiety.

• Journal of Ocean Engineering and Technology
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• v.29 no.2
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• pp.169-174
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• 2015

This paper addresses experimental methodologies to measure the temperature-dependent thermal conductivity of the insulation materials popularly used for LNG cargo containment systems. The measurement techniques considered in this paper are the guarded hot plate (GHP) method and heat flow method (HFM). The former is based on the power supplied to the hot plate to keep the temperature constant, and the latter is based on a direct heat flux measurement. In order to improve the accuracy of the HFM, the thermal conductivity obtained by GHP was cross-compared with the HFM results, and a calibration factor was derived. It was found that the thermal conductivities measured by the two methods corresponded well under room temperature, but the deviation tended to slightly increase as the temperature decreased. Because of the easy installation and operability of HFM, it can be used to measure thermal conductivity in a large scale mock-up test or unit insulation panel test, where the GHP method is difficult to apply.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.62 no.6
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• pp.833-839
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• 2013

Cardiopulmonary resuscitation (CPR) is performed by thoracic compression and artificial ventilation for the patient under emergent situation to maintain at least the minimum level of respiration and blood circulation for life survival. Good quality CPR requires monitoring respiration, however, traditional respiratory air flow transducers cannot be used because the transducer elements are facing the whole area perpendicular to the flow axis. The present study developed a new air flow transducer conveniently applicable to CPR. Specially designed "sensing rod" samples the air velocity at 3 different locations of the flow cross-section, then transforms into average dynamic pressure by the Bernoulli's law. The symmetric structure of the sensing holes of the sensing rod enables bi-directional measurement simply by taking the difference in pressure by a commercial differential pressure transducer. Both inspiratory and expiratory flows were obtained with symmetric measurement characteristics. Quadratic curve fitting provided excellent calibration formula with a correlation coefficient>0.999 (P<0.0001) and the mean relative error<1%. The present results can be usefully applied to accurately monitor the air flow rate during CPR.

• Proceedings of the KSME Conference
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• 2004.04a
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• pp.1951-1956
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• 2004

The transient nature and complex flow geometries of two-phase gas-liquid flows cause fundamental difficulties when measuring flow velocity using an electromagnetic flowmeter. Recently, a current-sensing flowmeter was introduced to obtain measurements with high temporal resolution (Ahn et $al.^{(1)}$). In this study, current-sensing flowmeter theory was applied to measure the fast velocity transients in slug flows. To do this, the velocity fields of axisymmetric gas-liquid slug flow in a vertical pipe were obtained using Volume-of-Fluid (VOF) method and the virtual potential distributions for the electrodes of finite size were also computed using the finite volume method for the simulated slug flow. The output signal prediction for slug flow was carried out from the velocity and virtual potential (or weight function) fields. The flowmeter was numerically calibrated to obtain the cross-sectional liquid mean velocity at an electrode plane from the predicted output signal. Two calibration parameters are required for this procedure: a flow pattern coefficient and a localization parameter. The flow pattern coefficient was defined by the ratio of the liquid resistance between the electrodes for two-phase flow with respect to that for single-phase flow, and the localization parameter was introduced to avoid errors in the flowmeter readings caused by liquid acceleration or deceleration around the electrodes. These parameters were also calculated from the computed velocity and virtual potential fields. The results can be used to obtain the liquid mean velocity from the slug flow signal measured by a current-sensing flowmeter.

• Journal of Korea Water Resources Association
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• v.43 no.3
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• pp.283-294
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• 2010

The objective of this study is to develop an accurate and stable dynamic water quality model which is capable of reflecting various flows and irregular cross sections and handling numerical oscillations under the low flow conditions. In order to solve the oscillation problem under the low flow conditions, diffusive wave method was applied to the low flow condition in developing a hydraulic model, DyHYD. DyQUAL is also developed as a water quality model to calculate up to 12 water quality variables including autochthonous BOD, water temperature, DO, TN and TP. The developed model is applied to both hypothetical river channels and actual Nakdong river watershed. Additionally, the applicability and reliability of the models are verified by comparing simulation results with observed values. Nash-Sutcliffe coefficients are estimated by comparison between simulation results and observed values. In the calibration and verification process, the coefficients varies from 0.391 to 0.591 and 0.704 to 0.902 for discharge, BOD, TN and TP, respectively.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.29 no.2
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• pp.118-127
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• 2018

In this work, we have designed and manufactured radar target simulation equipment for the performance analysis of synthetic aperture radar(SAR) systems. First, we have explained the function and performance specification of the target simulation equipment and point target scenario generation for validation of the SAR system. In addition, we have developed a simple and accurate calibration method for the time delay of the SAR system using the manufactured target simulation equipment. We have analyzed the point target impulse response function of the SAR image acquired using the SAR system and the target simulation equipment. It was observed that the measured peak to side lobe ratio(=-13.25 dB) and resolution(=0.49 m) are in good agreement with the corresponding theoretical values.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.1
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• pp.29-34
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• 2012

The objective of this study was to rapidly evaluate fatty acids in a collection of millet (Panicum miliaceum subsp. miliaceum) of different origins so that this information could be disseminated to breeders to advance germplasm use and breeding. To develop the calibration equations for rapid and nondestructive evaluation of fatty acid content, near-infrared reflectance spectroscopy (NIRs) spectra (1104-2494 nm) of samples ground into flour ($n$=100) were obtained using a dispersive spectrometer. A modified partial least-squares model was developed to predict each component. For foxtail millet germplasm, our models returned coefficients of determination ($R^2$) of 0.89, 0.89, 0.89, and 0.92 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively. The prediction of the external validation set (n=10) showed significant correlation between references values and NIRs values ($r^2$=0.64, 0.90, 0.79, and 0.89 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively). Standard deviation/standard errors of cross-validation (SD/SECV) values were close to 3 (2.62, 2.40, 1.85, and 2.23 for palmitic acid, oleic acid, linoleic acid, and total fatty acids, respectively). These results indicate that these NIRs equations are functional for the mass screening and rapid quantification of the oleic and total fatty acids characterizing millet germplasm. Among the samples, IT153514 showed an especially high content of fatty acids ($48.14mg\;g^{-1}$), whereas IT123909 had a very low content ($34.44mg\;g^{-1}$).

• Journal of the Korean Association of Geographic Information Studies
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• v.12 no.3
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• pp.21-34
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• 2009

Usually in flood flow problems, one-dimensional approach does not provide the required details of complex flow phenomena such as the flow in braided rivers and river junction. In this study, two-dimensional finite element mesh is constructed using DEM and GIS tool, and applied to RMA-2model. The purpose of this study is to investigate the applicability of the two dimensional model in natural rivers and to analyze characteristics of river flow due to the change of cross section. For model calibration, the result of unsteady flow analysis was compared with the observed data. Accordingly, the SMS model in this study prove to be very effective and reliable tool for the simulation of hydrodynamic characteristics under the various flow conditions.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.448-454
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• 2010

The objective of this study was to rapidly evaluate fatty acids in a collection of foxtail millet (Setaria italica (L.) P. Beauv) of different origins so that this information could be disseminated to breeders to advance germplasm use and breeding. To develop the calibration equations for rapid and nondestructive evaluation of fatty acid content, near-infrared reflectance spectroscopy (NIRs) spectra (1104-2494 nm) of samples ground into flour (n=100) were obtained using a dispersive spectrometer. A modified partial least-squares model was developed to predict each component. For foxtail millet germplasm, our models returned coefficients of determination ($R^2$) of 0.91, 0.89, 0.98 and 0.98 for strearic acid, oleic acid, linoleic acid, and total fatty acids, respectively. The prediction of the external validation set (n=10) showed significant correlation between references values and NIRs values ($r^2=0.97$, 0.91, 0.99 for oleic, linoleic, and total fatty acids, respectively). Standard deviation/standard error of cross-validation (SD/SECV) values were greater than 3 (3.11, 5.45, and 7.50 for oleic, linoleic, and total fatty acids, respectively). These results indicate that these NIRs equations are functional for the mass screening and rapid quantification of the oleic, linolenic, and total fatty acids characterizing foxtail millet germplasm. Among the samples, IT153491 showed an especially high content of fatty acids ($84.06mg\;g^{-1}$), whereas IT188096 had a very low content ($29.92mg\;g^{-1}$).

• Korean Journal of Veterinary Research
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• v.60 no.4
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• pp.209-213
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• 2020

This study examined the residue of dl-methylephedrine hydrochloride (MEP) on the muscle of pigs administered orally with MEP 12 g/ton feed for seven consecutive days. Twenty healthy cross swine were administered MEP. Four treated animals were selected arbitrarily to be sacrificed at 1, 2, 3, 4, and 5 days after treatment. MEP residue concentrations in the muscle were determined by liquid chromatography coupled with tandem mass spectrometry. The drug was extracted from muscle samples using 10 mM ammonium formate in acetonitrile followed by clean-up with n-hexane. The analyte was separated on an XBridgeTM hydrophilic interaction liquid chromatography column using 10 mM ammonium formate in deionized distilled water and acetonitrile. The correlation coefficient (R2) of the calibration curve was 0.9974, and the limits of detection and quantification were 0.05 and 0.15 ㎍/kg, respectively. The recoveries at three spiking levels were 94.5-101.2%, and the relative Standard Deviations was less than 4.06%. In the MEP-treated group, MEP residues on one day post-treatment were below the maximum residue limit in the muscle. The developed method is sensitive and reliable for the detection of MEP in porcine muscle tissues. Furthermore, it exhibits low quantification limits for animal-derived food products destined for human consumption.