• Journal of Microbiology and Biotechnology
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• v.30 no.11
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• pp.1688-1696
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• 2020

Soil physical and chemical characteristics, soil potential denitrification rates (PDR), community composition and nirK-, nirS- and nosZ-encoding denitrifiers were studied by using MiSeq sequencing, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment polymorphism (T-RFLP) technologies base on short-term (5-year) tillage field experiment. The experiment included four tillage treatments: conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), no-tillage with crop residue retention (NT), and rotary tillage with crop residue removed as control (RTO). The results indicated that soil organic carbon, total nitrogen and NH₄⁺-N contents were increased with CT, RT and NT treatments. Compared with RTO treatment, the copies number of nirK, nirS and nosZ in paddy soil with CT, RT and NT treatments were significantly increased. The principal coordinate analysis indicated that tillage management and crop residue returning management were the most and the second important factors for the change of denitrifying bacteria community, respectively. Meanwhile, this study indicated that activity and community composition of denitrifiers with CT, RT and NT treatments were increased, compared with RTO treatment. This result showed that nirK, nirS and nosZ-type denitrifiers communities in crop residue applied soil had higher species diversity compared with crop residue removed soil, and denitrifying bacteria community composition were dominated by Gammaproteobacteria, Deltaproteobacteria, and Betaproteobacteria. Therefore, it is a beneficial practice to increase soil PDR level, abundance and community composition of nitrogen-functional soil microorganism by combined application of tillage with crop residue management.

• Korean Journal of Plant Resources
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• v.27 no.2
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• pp.202-208
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• 2014

This study was carried out to find the effect of sowing time on oil content and fatty acid composition of 6 domestic rapeseed cultivars, 'Sunmang', 'Tammiyuchae', 'Tamlayuchae', 'Naehanyuchae', 'Yongsanyuchae' and'Hallayuchae'. The delaying sowing date was negatively correlated with oil content ($-0.471^{**}$), indicating that oil content was higher with earlier sowing date. Fatty acid composition was similar in all cultivars. The delaying sowing date was positively correlated with the increment of stearic acid ($0.268^*$) and linoleic acid ($0.263^*$), while was inversely correlated with palmitic acid ($-0.278^*$) across all 6 cultivars.

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.168-168
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• 2019

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.04a
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• pp.132-132
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• 2019

• Proceedings of the Korean Society of Crop Science Conference
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• 2021.04a
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• pp.105-105
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• 2021

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.2
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• pp.141-147
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• 2004

This study was carried out to know the variation of soybean seed proteins, 11S and 7S globulins, and their amino acid compositions among different colored soybean varieties, 'Danbaegkong' (yellow), 'Pureunkong' (green) 'Jinyulkong' (brown), and 'Geoumjeongkong l' (black). Soybean seed proteins showed a wide range in molecular size, but the electrophoresis patterns of total seed protein subunits showed a similarity among different colored soybean varieties. Amino acid compositions of total seed proteins were similar for all soybean varieties tested. However, soybean varieties showed low composition rates in sulfur containing amino acids. The composition rates of cysteine and methionine in the 11S globulins were higher than those of total seed proteins and 7S globulins. Glutamic acid and glycine were higher in the 11S and 7S globulins than those of total seed proteins. However, the levels of methionine and phenylalanine are high in the 11S globulins, but those of valine and lysin are slightly lower than the 7S globulins. By using HPLC, we tried to analyse the soybean seed proteins. The 11S globulin was composed of 10 major peaks whereas the 7S globulin was composed of 4 major peaks. The composition rates of 11S related proteins have a tendency to increasing during the maturing whereas those of 7S related proteins have a tendency to decreasing. Composition rates of each peaks among different colored soybean varieties suggested that soybean seed proteins are varied, although they showed similarity in the electrophoresis patterns, and understanding of this characteristics is important for the utilization of soybeans.

• Proceedings of the Korean Society of Crop Science Conference
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• 1993.10a
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• pp.32-33
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• 1993

• Proceedings of the Korean Society of Crop Science Conference
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• 1993.10a
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• pp.34-35
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• 1993

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.289-289
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• 2017

The climate change impact has facilitated double cropping system on maize production in Korea. The objectives of this study were to investigate the chemical composition changes according to the sowing dates on double cropping in 8 dent type, 2 intermediate type, and 4 semiflint type of Korean maize (Zea mays L.) hybrids and evaluate its antioxidant characteristics. Fourteen maize hybrids were sown on April 5 and July 5 of 2015. The average crude protein contents in dent and semiflint type maize sown on April 5 were higher than those sown on July 5 (p<0.05). There was no significant difference in average of total amino acid contents in all types of maize according to the sowing dates. Major amino acid in maize hybrids were glutamic acid followed by proline, alanine, and aspartic acid, which has no significant difference according to the sowing dates. The average crude fat contents in semiflint and intermediate type maize sown on April 5 were higher than those sown on July 5 (p<0.05). The average composition of saturated fatty acid in dent type maize sown on April 5 was higher than those sown on July 5. However, the average unsaturated fatty acid composition showed the opposite result (p<0.05). Fatty acids were mainly composed of linoleic acid (C18:2) and oleic acid (C18:1) in maize hybrids. The average oleic acid percentage of dent and semiflint type maize sown on April 5 were higher than those sown on July 5, while the average linoleic acid was lower. The average amylose content of all types of maize sown on April 5 was higher than those sown on July 5. On the other hands, the average carotenoid contents had the opposite result (p<0.05). There were no significant differences in total average of polyphenol contents and DPPH and ABTS radical scavenging activities in all types of maize based on the sowing date. Total polyphenol contents had positive correlation with DPPH (r=0.33, p<0.01) and ABTS (r=0.50, p<0.0001) radical scavenging activities. In conclusion, the kernel composition affects maize quality. These data are useful for maize breeding program and cultivation and food processing industry.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.1
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• pp.32-38
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• 2005

A long-term study initiated in 1989 at San-born Field, Columbia, Missouri, was designed to evaluate the affect of environmental factors, nitrogen application, and crop rotation on soybean (Glycine max [L.] Merr.) seed composition. Soybeans were grown as part of a four- year rotation which included corn (Zea maize L.), wheat (Triticum aestivum L.), and red clover (Trifolium pratense L.). Results from soil tests made prior to initiation of the study and subsequently every five years, were used to calculate application rates of nitrogen, phosphorus, and potassium necessary for target yield of pursuant crops. In the experimental design, nitrogen was applied to one-half of the plot on which the non-leguminous crop, either corn or wheat was grown. Analysis of soybean seed by near infrared reflectance spectroscopy collected over an 11-year period revealed a linear increase in protein and decrease in oil content. Application of nitrogen fertilizer to non-leguminous crops did not have an apparent effect on total protein or oil content of subsequent soybean crop. Analysis of soybean seed proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis in conjunction with computer­assisted densitometry revealed subtle changes in the accumulation of seed proteins. Immunoblot analysis using antibodies raised against the $\beta-subunit$ of $\beta-conglycinin$ showed a gradual increase in the accumulation of the 7S components during successive years of the experiment. A linear increase in temperature and decrease in rainfall was observed from the onset of data· collection. Higher temperatures during the growing season have been linked to increased protein and diminished oil content of soybean, thus changes observed in this study are possibly related to climatic conditions. However, crop rotation and subsequent changes in soil ecology may contribute to these observed changes in the seed composition.