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http://dx.doi.org/10.4014/jmb.2007.07012

Effects of Short-Term Soil Tillage Management on Activity and Community Structure of Denitrifiers under Double-Cropping Rice Field  

Tang, Haiming (Hunan Soil and Fertilizer Institute)
Li, Chao (Hunan Soil and Fertilizer Institute)
Cheng, Kaikai (Hunan Soil and Fertilizer Institute)
Shi, Lihong (Hunan Soil and Fertilizer Institute)
Wen, Li (Hunan Soil and Fertilizer Institute)
Xiao, Xiaoping (Hunan Soil and Fertilizer Institute)
Xu, Yilan (Hunan Biological and Electromechanical Polytechnic)
Li, Weiyan (Hunan Soil and Fertilizer Institute)
Wang, Ke (Hunan Soil and Fertilizer Institute)
Publication Information
Journal of Microbiology and Biotechnology / v.30, no.11, 2020 , pp. 1688-1696 More about this Journal
Abstract
Soil physical and chemical characteristics, soil potential denitrification rates (PDR), community composition and nirK-, nirS- and nosZ-encoding denitrifiers were studied by using MiSeq sequencing, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment polymorphism (T-RFLP) technologies base on short-term (5-year) tillage field experiment. The experiment included four tillage treatments: conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), no-tillage with crop residue retention (NT), and rotary tillage with crop residue removed as control (RTO). The results indicated that soil organic carbon, total nitrogen and NH4+-N contents were increased with CT, RT and NT treatments. Compared with RTO treatment, the copies number of nirK, nirS and nosZ in paddy soil with CT, RT and NT treatments were significantly increased. The principal coordinate analysis indicated that tillage management and crop residue returning management were the most and the second important factors for the change of denitrifying bacteria community, respectively. Meanwhile, this study indicated that activity and community composition of denitrifiers with CT, RT and NT treatments were increased, compared with RTO treatment. This result showed that nirK, nirS and nosZ-type denitrifiers communities in crop residue applied soil had higher species diversity compared with crop residue removed soil, and denitrifying bacteria community composition were dominated by Gammaproteobacteria, Deltaproteobacteria, and Betaproteobacteria. Therefore, it is a beneficial practice to increase soil PDR level, abundance and community composition of nitrogen-functional soil microorganism by combined application of tillage with crop residue management.
Keywords
Tillage; crop residue; paddy field; soil denitrification rate; community composition;
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  • Reference
1 Elder JW, Lal R. 2008. Tillage effects on gaseous emissions from an intensively farmed organic soil in North Central Ohio. Soil Tillage Res. 98: 45-55.   DOI
2 Rochette P. 2008. No-till only increases N2O emissions in poor lyaerated soils. Soil Tillage Res. 101: 97-100.   DOI
3 Wang WY, Yang M, Shen PF, Zhang RY, Qin XL, Han J, et al. 2019. Conservation tillage reduces nitrous oxide emissions by regulating functional genes for ammonia oxidation and denitrification in a winter wheat ecosystem. Soil Tillage Res. 194: 104347.   DOI
4 Tatti E, Goyer C, Burton DL, Wertz S, Zebarth BJ, Chantigny M, et al. 2015. Tillage management and seasonal effects on denitrifier community abundance, gene expression and structure over winter. Microb. Ecol. 70: 795-808.   DOI
5 Kandeler E, Deiglmayr K, Tscherko D, Bru D, Philippot L. 2006. Abundance of narG, nirS, nirK, and nosZ genes of denitrifying bacteria during primary successions of a glacier foreland. Appl. Environ. Microbiol. 72: 5957-5962.   DOI
6 Jones CM, Stres B, Rosenquist M, Hallin S. 2008. Phylogenetic analysis of nitrite, nitric oxide, and nitrous oxide respiratory enzymes reveal a complex evolutionary history for denitrification. Mol. Biol. Evol. 25: 1955-1966.   DOI
7 Avrahami S, Conrad R, Braker G. 2002. Effect of soil ammonium concentration on N2O release and on the community structure of ammonia oxidizers and denitrifiers. Appl. Environ. Microbiol. 68: 5685-5692.   DOI
8 Cheneby D, Brauman A, Rabary B, Philippot L. 2009. Differential responses of nitrate reducer community size, structure, and activity to tillage systems. Appl. Environ. Microbiol. 75: 3180-3186.   DOI
9 Masuda Y, Matsumoto T, Isobe K, Senoo K. 2019. Denitrification in paddy soil as a cooperative process of different nitrogen oxide reducers, revealed by metatranscriptomic analysis of denitrification-induced soil microcosm. Soil Sci. Plant Nutr. 65: 342-345.   DOI
10 Lehmann J, Rillig MC, Thies J, Masiello CA, Hockaday WC, Crowley D. 2011. Biochar effects on soil biota-a review. Soil Biol. Biochem. 43: 1812-1836.   DOI
11 SImek M, Cooper J. 2002. The influence of soil pH on denitrification: progress towards the understanding of this interaction over the last 50 years. Eur. J. Soil Biol. 53: 345-354.
12 Yang XY, Ren WD, Sun BH, Zhang SL. 2012. Effects of contrasting soil management regimes on total and labile soil organic carbon fractions in a loess soil in China. Geoderma 177-178: 49-56.   DOI
13 Yu ZH, Liu JJ, Li Y S, Jin J, Liu XB, Wang GH. 2018. Impact of land use, fertilization and seasonal variation on the abundance and diversity of nirS-type denitrifying bacterial communities in a Mollisol in Northeast China. Eur. J. Soil Biol. 85: 4-11.   DOI
14 Yoshida M, Ishii S, Otsuka S, Senoo, K. 2009. Temporal shifts in diversity and quantity of nirS and nirK in a rice paddy field soil. Soil Biol. Biochem. 41: 2044-2051.   DOI
15 Yin C, Fan F, Song A, Cui P, Li T, Liang Y. 2015. Denitrification potential under different fertilization regimes is closely coupled with changes in the denitrifying community in a black soil. Appl. Microbiol. Biotechnol. 99: 5719-5729.   DOI
16 Drury CF, Myrold DD, Beauchamp EG, Reynolds WD. 2007. Denitrification techniques for soils. In Carter MR, Gregorich ED (eds.), pp. 471-493. Soil sampling and methods of analysis, 2nd ed. CRC Press, Boca Raton.
17 Tang HM, Xiao XP, Li C, Tang WG, Cheng KK, Pan XC, et al. 2019. Effects of different soil tillage systems on soil carbon management index under double-cropping rice field in southern China. Agron. J. 111: 440-446.   DOI
18 Bao SD. 2000. Soil and Agricultural Chemistry Analysis. Pp. 49-56. China Agriculture Press, Beijing.
19 Molstad L, Dorsch P, Bakken LR. 2007. Robotized incubation system for monitoring gases (O2, NO, N2O and N2) in denitrifying cultures. J. Microbiol. Methdos 71: 202-211.   DOI
20 Throback IN, Enwall K, Jarvis A, Hallin S. 2004. Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE. FEMS Microbiol. Ecol. 49: 401-417.   DOI
21 Long X, Chen C, Xu Z, Linder S, He JZ. 2012. Abundance and community structure of ammonia oxidizing bacteria and archaea in a Sweden boreal forest soil under 19-year fertilization and 12-year warming. J. Soils Sediments 12: 1124-1133.   DOI
22 Jung J, Yeom J, Kim J, Han J, Lim HS, Park H, et al. 2011. Change in gene abundance in the nitrogen biogeochemical cycle with temperature and nitrogen addition in Antarctic soils. Res. Microbiol, 162: 1018-1026.   DOI
23 Zumft W. 1997. Cell biology and molecular basis of denitrification. Microbiol. Mol. Biol. Rev. 61: 533-616.   DOI
24 Zeng J, Liu XJ, Song L, Lin XG, Zhang HY, Shen CC, et al. 2016. Nitrogen fertilization directly affects soil bacterial diversity and indirectly affects bacterial community composition. Soil Biol. Biochem. 92: 41-49.   DOI
25 SAS Institute Inc. 2013. SAS 9.4 in-database products: user's guide. 3rd ed. SAS Institute Inc.; Cary, NC.
26 Enwall K, Philippot L, Hallin S. 2005. Activity and composition of the denitrifying bacterial community respond differently to longterm fertilization. Appl. Environ. Microbiol. 71: 8335-8343.   DOI
27 Cao YP, Green PG, Holden PA. 2008. Microbial community composition and denitrifying enzyme activities in salt marsh sediments. Appl. Environ. Microbiol. 74: 7585-7595.   DOI
28 Henry S, Baudoin E, Lopez-Gutierrez JC, Martin-Laurent F, Baumann A, Philippot L. 2004. Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR. J. Microbiol. Methods 59: 327-335.   DOI
29 Chen Z, Luo X, Hu R, Wu M,Wu J, Wei W. 2010. Impact of long-term fertilization on the composition of denitrifier communities based on nitrite reductase analyses in a paddy soil. Microb. Ecol. 60: 850-861.   DOI
30 Braker G, Zhou J, Wu L, Devol AH, Tiedje JM. 2000. Nitrite reductase genes (nirK and nirS) as functional markers to investigate diversity of denitrifying bacteria in Pacific northwest marine sediment communities. Appl. Environ. Microbiol. 66: 2096-2104.   DOI
31 Schulz S, Kolbl A, Ebli M, Buegger F, Schloter M, Fiedler S. 2017. Field-scale pattern of denitrifying microorganisms and N2O emission rates indicate a high potential for complete denitrification in an agriculturally used organic soil. Microb. Ecol. 74: 765-770.   DOI
32 Li S, Song L, Jin Y, Liu S, Shen Q, Zou JW. 2016. Linking N2O emission from biochar-amended composting process to the abundance of denitrify (nirK and nosZ) bacteria community. AMB Exp. 6: 37.   DOI
33 Yang L, Zhang X, Ju X. 2017. Linkage between N2O emission and functional gene abundance in an intensively managed calcareous fluvo-aquic soil. Sci. Rep. 7: 43283.   DOI