• Journal of Embryo Transfer
• /
• v.9 no.1
• /
• pp.1-6
• /
• 1994

This experiment was investigated the effect of presence of granulosa cells from follicles of different size on bovine oocyte maturation, cleavage and development to late stage. The nuclear and cytoplasmic maturation of oocytes in the IVM-IVF system are critical for subsequent embryo development. Granulosa cells when the co-cultured with oocytes may interact with cumulus-oocytes complexes and influence the development competence of the oocytes. Granulosa cells from medium (2~6 mm) and large(>1O mm) size follicles were recovered by aspiration, washed 3 times by centrifugation at 500 x g for 5 min. and used for co-culture at a concentration of 2~3 x 106 cells/mi. The oocytes were matured in vitro (IVM) for 24 hrs. in TCM-199 supplemented with 35 $\mu$g/ml FSH, 10 $\mu$g/ml LH, 1 $\mu$g/ml estradiol-17$\beta$ and granulosa cells at 39$^{\circ}C$ under 5% $CO_2$ in air. They were fertilized in vitro (IVF) by epididymal spermatozoa treated with heparin for 24 hrs., and then the zygotes were co-cultured in vitro (I VC) with bovine oviductal epithelial cells for 7 to 9 days. The assessment of maturation revealed that Grade J oocytes showed significantly(P

• Journal of the Korean Society for Nondestructive Testing
• /
• v.32 no.2
• /
• pp.122-130
• /
• 2012

Adhesive bend and shear tests of micro-sized bonded component have been performed to clarify the relationship between effects of heat-treatment on the adhesive strength and the bonded specimen shape using Weibull analysis. Multiple micro-sized SU-8 columns with four different diameters were fabricated on a Si substrate under the same fabrication condition. Heat-treatment can improve both of the adhesive bend and shear strength. The improvement rate of the adhesive shear strength is much larger than that of the adhesive bend strength, because the residual stress, which must change by heat-treatment, should effect more strongly on the shear loading. In case of bend type test, the adhesive bend strength in the smaller diameters (50 and $75\;{\mu}m$) widely vary, because the critical size of the natural defect (micro-crack) should vary more widely in the smaller diameters. In contrast, in case of shear type test, the adhesive shear strengths in each diameter of the columns little vary. This suggests that the size of the natural defects may not strongly influence on the adhesive shear strength. All the result suggests that both of the adhesive bend and shear strengths should be complicatedly affected by heat-treatment and the bonded columnar diameter.

• Journal of the Korean Institute of Gas
• /
• v.14 no.2
• /
• pp.47-52
• /
• 2010

The development of energy saving system with pressure regulator has recently been received interests. Since the internal geometry in the pressure regulator is small and complex, the frictional loss in it is critical. In this study, the pressure loss with tip size, tip position, and mass flow was investigated using numerical approaches(CFD). The aimed reduction in pressure were achieved as the ratio of t/T was more than 0.8. In addition, there was no effect of the tip position.

• Ocean and Polar Research
• /
• v.27 no.3
• /
• pp.323-333
• /
• 2005

The paper is to serve as a guide for deciding on a desirable direction for the vessel buyback program, reviewing their performance and economic effects from fish harvest reduction due to not reaching MSY. Compared with maximum willingness to pay for vessel buyback programs designed to avoid economic losses occurring as a result of overfishing, the portions of investment costs for buyback program are so small comparatively to the range of 0.32% to 12.19% at the annual base. The economic loss effects occurred in terms of the reduction of fishing harvest are comprehensively estimated at the present value of 30,877 billion won since 1971, and exceeded the revenues for fish harvests from 1999. In order to resole fish stocks through a vessel buyback program, this paper recommends that the yield should be reduced to less than the intrinsic growth rate. Otherwise, the buyback program policy eventually fails regardless of the temporal effect of benefits. This paper further argues that technical policy tools such as fishing grounds, fishing seasons, size of fish and minimum size of meshes should be effectively utilized.

• International Journal of Highway Engineering
• /
• v.19 no.4
• /
• pp.37-44
• /
• 2017

PURPOSES : Conductive and convective heat transfer simulations for an asphalt mixture were made by using discrete element method (DEM) and similarity principle. METHODS : In this research, virtual specimens composed of discrete element method particles were generated according to four different predetermined particle size distribution curves. Temperature variations of the four different particles for a given condition were estimated and were compared with measurements and analytical solutions. RESULTS : The virtual specimen with mixed particles and with the smallest particle show very good agreement with laboratory test results and analytical solutions. As particle size decreases, better heat transfer simulation can be performed due to smaller void ratio and more contact points and areas. In addition, by utilizing the similarity principle of thermal properties and corresponding time unit, analytical time can be drastically reduced. CONCLUSIONS : It is concluded that the DEM asphalt mixture specimens with similarity principle could be used to predict the temperature variation for a given condition. It is observed that the void ratio has critical effect on prediction of temperature variation. Comparing the prediction for a 4 mm particle specimen with a mixed particle specimen, it is also concluded that predicting the mixed particle specimen temperature is much more efficient considering the number of particles that are directly associated with computational time in DEM analysis.

• Journal of Advanced Marine Engineering and Technology
• /
• v.35 no.4
• /
• pp.468-474
• /
• 2011

The flow field around baffle located sudden expansion and contraction channel was measured by PIV method and the effect according to height change of baffle built in the flow field was evaluated. The inlet flow velocity and the baffle height influence mutually to the size and flow pattern of the recycle flow of the back of the baffle and the size of the area of the water power jump passing the upper part of the baffle. In case of Reynolds number $Re=4{\times}10^3$, the critical value of baffle height is estimated around h/H=1.6 and there was a decreasing tendency as the inlet flow velocity was increased.

• Journal of the Korean Applied Science and Technology
• /
• v.10 no.1
• /
• pp.49-55
• /
• 1993

Ground Calcium Carbonate, among paper coating pigments, will influence less dispersant demand, less binder demand, increase coating solids from 58% to 70%, which means high speed coating, less shrinkage during drying, less energy consumption, more uniform coverage of fibers. The quality point of view of Ground Calcium Carbonate, brightness, particle size, Particle size distribution, hardness, impurities content are important. More important factors of Ground Calcium Carbonate which influence the paper coating process are dispersion mechanisms and their effects. The study was made to investigate the effect of Ground Calcium Carbonate dispersion by sodium salt of polyacrylate dispersant composition and dispersion condition. Basic tests such as physical, optical and chemical were perfumed, and dispersion effects were investigated by different conditions. The results showed that the type of dispersant affected the dispersion effects, and the Ground Calcium Carbonate has critical dispersant demand.

• IMMUNE NETWORK
• /
• v.12 no.3
• /
• pp.104-112
• /
• 2012

Silica is one of the most abundant compounds found in nature. Immoderate exposure to crystalline silica has been linked to pulmonary disease and crystalline silica has been classified as a Group I carcinogen. Ultrafine (diameter <100 nm) silica particles may have different toxicological properties compared to larger particles. We evaluated the effect of ultrafine silica nanoparticles on mouse bone marrow-derived dendritic cells (BMDC) and murine dendritic cell line, DC2.4. The exposure of dendritic cells (DCs) to ultrafine silica nanoparticles showed a decrease in cell viability and an induction of cell death in size- and concentration-dependent manners. In addition, in order to examine the phenotypic changes of DCs following co-culture with silica nanoparticles, we added each sized-silica nanoparticle along with GM-CSF and IL-4 during and after DC differentiation. Expression of CD11c, a typical DC marker, and multiple surface molecules such as CD54, CD80, CD86, MHC class II, was changed by silica nanoparticles in a size-dependent manner. We also found that silica nanoparticles affect inflammatory response in DCs in vitro and in vivo. Finally, we found that p38 and NF-${\kappa}B$ activation may be critical for the inflammatory response by silica nanoparticles. Our data demonstrate that ultrafine silica nanoparticles have cytotoxic effects on dendritic cells and immune modulation effects in vitro and in vivo.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2003.06a
• /
• pp.620-623
• /
• 2003

The material processing by using ultrashort pulse laser, in recently, is actively applying into the micro machining and nano-machining technology since ultrashort pulse has so faster than the time which the electrons energy absorbing photon energy is transmitted to surrounding lattice-phonon that it has many advantages in point of machining. The micro machining of metallic thin film on the plain glass is widely used in the fields such as mask repairing for semiconductor, fabrication of photonic crystal, MEMS devices and data storage devices. Therefore, it is important to secure the machining technology of the sub-micron size. In this research, we set up the machining system by using ultrashort pulse laser and conduct on the Cr 200nm thin film ablation experiments of spot and line with the variables such as energy, pulse number, speed, and so on. And we observed the characteristics of surrounding heat-affected zone and by-products appeared in critical energy density and higher energy density through SEM, and also examined the machining features between in He gas atmosphere which make pulse change minimized by nonlinear effect and in the air. Finally, the pit size of 0.8${\mu}{\textrm}{m}$ diameter and the line width of 1${\mu}{\textrm}{m}$ could be obtained.

• Journal of Korea Foundry Society
• /
• v.14 no.5
• /
• pp.438-446
• /
• 1994

The effect of various thermomechanical treatments on the structure and rheological behaviour of Al-6.2wt%Si alloy in its solidification range were investigated using a Searle type high temperature viscometer. During continuous cooling, the viscosity increases gradually with increasing fraction of solidified alloy, until a critical fraction of solidified alloy is reached above which the viscosity sharply increases. The viscosity of the slurry, at a given volume fraction wolid, decreased with increasing shear rate. The size and morphology of primary solid particles during stirring is influenced strongly by shear rates, cooling rates, volume fraction and stirring time of solid. Morphological changes during stirring as a function of solid volume fractions, shear rate and processing time were also reported. In this study, the size of primary solid particles in these alloys consistently increases and the it`s aspect ratio decrease with the increase in fraction solid and decrease in shear rate. Crystal morphology changes from rosette type to spheroid type with the increase in shear rate and solid fraction.