• Molecules and Cells
• /
• v.43 no.3
• /
• pp.286-297
• /
• 2020

Mammalian patatin-like phospholipase domain containing proteins (PNPLAs) play critical roles in triglyceride hydrolysis, phospholipids metabolism, and lipid droplet (LD) homeostasis. PNPLA7 is a lysophosphatidylcholine hydrolase anchored on the endoplasmic reticulum which associates with LDs through its catalytic region (PNPLA7-C) in response to increased cyclic nucleotide levels. However, the interaction of PNPLA7 with LDs through its catalytic region is unknown. Herein, we demonstrate that PNPLA7-C localizes to the mature LDs ex vivo and also colocalizes with pre-existing LDs. Localization of PNPLA7-C with LDs induces LDs clustering via non-enzymatic intermolecular associations, while PNPLA7 alone does not induce LD clustering. Residues 742-1016 contains four putative transmembrane domains which act as a LD targeting motif and are required for the localization of PNPLA7-C to LDs. Furthermore, the N-terminal flanking region of the LD targeting motif, residues 681-741, contributes to the LD targeting, whereas the C-terminal flanking region (1169-1326) has an anti-LD targeting effect. Interestingly, the LD targeting motif does not exhibit lysophosphatidylcholine hydrolase activity even though it associates with LDs phospholipid membranes. These findings characterize the specific functional domains of PNPLA7 mediating subcellular positioning and interactions with LDs, as wells as providing critical insights into the structure of this evolutionarily conserved phospholipid-metabolizing enzyme family.

• Smart Structures and Systems
• /
• v.15 no.1
• /
• pp.99-117
• /
• 2015

The Stonecutters Bridge (SCB) in Hong Kong is the third-longest cable-stayed bridge in the world with a main span stretching 1,018 m between two 298 m high single-leg tapering composite towers. A Wind and Structural Health Monitoring System (WASHMS) is being implemented on SCB by the Highways Department of The Hong Kong SAR Government, and the SCB-WASHMS is composed of more than 1,300 sensors in 15 types. In order to establish a linkage between structural health monitoring and maintenance management, a Structural Health Rating System (SHRS) with relevant rating tools and indices is devised. On the basis of a 3D space frame finite element model (FEM) of SCB and model updating, this paper presents the development of an SHR-oriented 3D multi-scale FEM for the purpose of load-resistance analysis and damage evaluation in structural element level, including modeling, refinement and validation of the multi-scale FEM. The refined 3D structural segments at deck and towers are established in critical segment positions corresponding to maximum cable forces. The components in the critical segment region are modeled as a full 3D FEM and fitted into the 3D space frame FEM. The boundary conditions between beam and shell elements are performed conforming to equivalent stiffness, effective mass and compatibility of deformation. The 3D multi-scale FEM is verified by the in-situ measured dynamic characteristics and static response. A good agreement between the FEM and measurement results indicates that the 3D multi-scale FEM is precise and efficient for WASHMS and SHRS of SCB. In addition, stress distribution and concentration of the critical segments in the 3D multi-scale FEM under temperature loads, static wind loads and equivalent seismic loads are investigated. Stress concentration elements under equivalent seismic loads exist in the anchor zone in steel/concrete beam and the anchor plate edge in steel anchor box of the towers.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.18 no.3
• /
• pp.37-42
• /
• 2010

Plastic region tightening is widely used in critical bolted joints in internal combustion engines in order to reduce the engine weight by maximizing the use of load-carrying capacity of bolt. Mechanical behavior of bolt tightened in plastic region under external axial tensile load is investigated for various friction conditions using three dimensional finite element analysis. The behavior of bolt tightened in elastic region as well as that in tensile test are investigated for comparison. Tightening process is simulated by rotating the bolt in order to examine the friction effect realistically. It is revealed that the bolt tightened in plastic region can carry more external load until the joint is opened, and yields at lower bolt load than the bolt tightened in elastic region. The friction coefficient has effect on the yield load, but not on the load-carrying capacity. Moreover, the scatter in the bolt preload due to friction begins with plastic deformation of bolt in the angle tightening control, whereas it begins with the onset of tightening in the torque tightening control. The observations are interpreted with the residual torsional stress in the bolt generated during the tightening.

• Journal of the Korean Institute of Telematics and Electronics C
• /
• v.36C no.10
• /
• pp.29-38
• /
• 1999

This paper presents a new algorithm RICP(Redundancy Identification using Critical-pair Paths) to identify untestable faults in combinational logic circuits. In a combinational logic circuit, untestable faults occurred by redundancy of circuits. The redundancy of a circuit can be detected by analyzing areas of fanout stem and reconvergent gates. The untestable faults are identified by analyzing stem area using Critical-Pair path which is an extended concept of critical path. It is showed that RICP is better than FIRE(Fault Independent REdundancy identification) algorithm in efficiency. The performance of both algorithms was compared using ISCAS85 bench mark testing circuits.

• Nuclear Engineering and Technology
• /
• v.55 no.9
• /
• pp.3313-3319
• /
• 2023

A phenomenological study on CHF in a bilaterally heated annulus with equal heat flux on both sides was experimentally performed. The working fluid of the present test was R-134a. Variation characteristics of CHF and transition of CHF occurrence location were investigated under different pressure, mass flux and quality conditions. With the increase of critical thermodynamic quality, it was found that CHF first occurred on the outer surface of the annulus, then simultaneously occurred on both sides, and finally occurred on the inner surface at relatively high critical quality. After the CHF location transitioned to the inner rod, the sharp fall of CHF in the limiting critical quality region was observed. The critical quality corresponding to the CHF location transition decreased with the increase of mass flux and pressure. Besides, CHF in tube, internally heated, externally heated and bilaterally heated annuli were compared under the same hydraulic diameter conditions. The present study is conducive to improving the understanding of complicated CHF mechanism in bilaterally heated annulus, enriching the experimental database, and providing evidence for developing accurate CHF mechanism model for annuli.

• Proceedings of the Korean Institute of Intelligent Systems Conference
• /
• 2006.05a
• /
• pp.199-202
• /
• 2006

We propose some properties of fuzzy p-value and fuzzy significance level to the test statistics for the fuzzy hypotheses testing. Appling the principle of agreement index, we suggest two method for fuzzy hypothesis testing by fuzzy rejection region and fuzzy p-value with fuzzy hypothesis $H_{f,0}$.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2006.08a
• /
• pp.393-398
• /
• 2006

This paper classifies driving technologies for AMOLEDs by the driving TFT conditions in pixels. A saturation region operation type driving TFT circuit provides good stability of OLED because of constant current drive. However, complicated compensation circuits are necessary to avoid effect of the TFT characteristics deviation. On the other hand, a linear region operation type driving TFT circuit provides better uniformity of the display image and lower power consumption. However, the stability of OLED is critical because of constant voltage drive.

• Proceedings of the KIPE Conference
• /
• 1998.10a
• /
• pp.847-850
• /
• 1998

This paper proposes a new scheme of FLR for improving corner breakdown voltage. The major difference from the conventional FLR is to build extra rings and floating field plates in the corner region. In this structure the additional field plate and ring have reduced th electric field at the junction in the corner region. Thus it improves the breakdown characteristics which are critical for obtaining high breakdown voltage.

• Journal of the Korean Geographical Society
• /
• v.39 no.1
• /
• pp.153-168
• /
• 2004

This study begins with the interests of the problems in learning a region under the Korean National Curriculum in geography field and the perceptions on the region which affects students 'formation of regional concept. The purposes of this study are to find problems in learning a region and to construct a new content for learning a region to improve the current problems. This study suggests that it is necessary for student to establish a clear concept on a region in order to effectively learn a region. Therefore, this study reviews how concepts on a region have changed and selects important elements in learning a region by considering a level of students' understanding. The important elements selected in this study are the definition of a region, the formation of a region, and the system of a region. By learning a real region established as a 'region for instruction' of a medium scale, students can reach an appropriate level of meaningful understanding of a region. This study found that an appropriate selection of scale in choosing the extent of a region was critical in success of effective organization of learning contents.

• Journal of Advanced Marine Engineering and Technology
• /
• v.24 no.5
• /
• pp.25-32
• /
• 2000

In this paper, experiments investigating the high-temperature region heat transfer coefficients for the spray cooling of hot flat plates were performed by down spray water using flat spray nozzles. The heat transfer surface is made of copper and is 100mm in length and 40mm in width and 15mm in thickness. The experimental condition of spray are as follows: temperatures of the water droplets are T=20~$80^{\circ}C$ and droplets volume fluxes are D=0.001565~0.010438$m^3/m^2s$. Next, correlating equations for the heat transfer characteristics of spray cooling in the high temperature region are developed from the effects of droplets volume flux and the surface temperature of heat transfer plate.