• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.37-37
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• 2018

The genus Crepidiastrum (Asteraceae), containing ca. 20 species, is mainly distributed in Asia. Crepidiastrum denticulatum, an edible plant that commonly call "e-go-deulppae-gi" in Korean, distributes in Korea, Japan, and China. The complete chloroplast (cp) genome sequences of C. denticulatum was characterized from MiSeq2000 (Illumina Co.) pair-end sequencing data. The cp genome of C. denticulatum has a total sequence length of 152,689 bp and show a typical quadripartite structure. It consists of the large single copy (LSC: 84,022 bp), small single copy (SSC: 18,519 bp), separated by a pair of inverted repeats (IRs: 25,074 bp) and contains 110 unique genes and 18 genes duplicated in the IR regions. Our comparative analysis identified three cpDNA regions (matK, rbcL, and psbA-trnH) from three Crepidiastrum species, which may be useful for molecular identification of each species, and providing a guideline for its clear confirming about dried medical herb.

• Korean Journal of Plant Taxonomy
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• v.52 no.2
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• pp.85-96
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• 2022

The plant "Hong-do-go-deul-ppae-gi" has been considered as Crepidiastrum × muratagenii, a hybrid between C. denticulatum and C. lanceolatum, based on its morphological traits and geographical distribution. To reveal the hybrid origin of Hong-do-go-deul-ppae-gi, we examined additional morphological traits of this plant and its putative parents (C. denticulatum, C. lanceolatum, C. platyphyllum) and analyzed one nuclear ribosomal internal transcribed spacer (ITS) region and four chloroplast regions (trnT-L, trnL-F, rpl16 intron, and rps16 intron). As a result of examining the morphological traits, putative hybrid individuals were classified into three types based on the habit, cauline leaf, outer phyllary, and achene beak traits. A molecular analysis found that the ITS sequences of Type 1 and Type 2 individuals showed additive species-specific sites of C. denticulatum and C. lanceolatum. Plastid sequences of Type 1 and Type 2 individuals showed C. denticulatum and C. lanceolatum sequences, respectively. However, Type 3 individuals had ITS and plastid sequences corresponding to C. denticulatum. Accordingly, Type 1 and Type 2 individuals not only share morphological traits with C. denticulatum and C. lanceolatum but also show additive species-specific sites for C. denticulatum and C. lanceolatum, and not C. platyphyllum, supporting its origin as a hybrid between C. denticulatum and C. lanceolatum. Type 3 had morphological traits similar to other hybrid types but was distinguished with respect to outer phyllaries and demonstrated some resemblance to C. denticulatum. In a molecular analysis, Type 3 was found to be identical with regard to the sequence of C. denticulatum and was judged to be an ecological variation of C. denticulatum.

• Korean Journal of Plant Taxonomy
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• v.51 no.3
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• pp.198-204
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• 2021

The plant known as "Hong-do-go-deul-ppae-gi" on Hongdo Island, belonging to the genus Crepidiastrum, has been recorded as C. ×nakaii, a hybrid between C. denticulatum and C. platyphyllum. During a survey of the flora of Hongdo Island, we reexamined the taxonomic identity of "Hong-do-go-deul-ppae-gi". The morphological traits, geographical distribution, and internal transcribed spacer (ITS) sequences of "Hong-do-go-deul-ppae-gi" were compared to related species within the genus. A morphological examination revealed that the plant was similar to C. lanceolatum in that it has subshrub stems with caudex stout, 8 inner involucrals bracts, and 10 to 12 florets. In contrast, similarities to C. denticulatum were observed in terms of radical leaves deciduous in the flowering period, cauline leaves membranous with acute serrations, and beaked achenes. Furthermore, C. denticulatum and C. lanceolatum grew on Hongdo Island and nearby areas, but C. platyphyllum was not distributed in these areas. The results of morphological and distributional investigations revealed that the Hongdo Island plant should be regarded as C. ×muratagenii, a hybrid between C. denticulatum and C. lanceolatum, not C. ×nakaii, which is a hybrid between C. denticulatum and C. platyphyllum. However, the resolution of the ITS sequences was insufficient such that we could not separate the examined species.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.5 no.2
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• pp.68-70
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• 2024

The genus Charops is one of the small genera of the subfamily Campopleginae with 30 species reporded worldwide. All of the known host insects of Charops striatus belong to the lepidopteran family Zygaenidae, perceived as harmful. C. striatus, one of the endoparasitoids from Elcysma westwoodi, is the most damaging lepidopteran pest of Prunus yedoensis. In South Korea, host plants of C. striatus were reported by Choi & Lee in 2008. In this paper, two new host plants, Crepidiastrum denticulatum and Zea mays, of Charops striatus are reporded for the first time from South Korea. Digital images and redescription of this species are provided.

• Food Science and Preservation
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• v.31 no.2
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• pp.276-286
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• 2024

In the present study, we investigated whether a mixture of fucoidan and Crepidiastrum denticulatum extract (FCE) had the potential to improve the therapeutic efficacy of cancer treatment. The results demonstrated that FCE significantly reduced cell viability and induced the release of LDH (lactate dehydrogenase) and DNA fragmentation in HepG2 cells in a dose-dependent manner. In addition, FCE treatment also increased the protein expression level of p53, the release of cytochrome c, and the loss of mitochondrial membrane potential. Moreover, FCE dose-dependently increased protein expression levels of Bax, and cleaved caspase-3 and -9. However, FCE decreased the protein expression level of Bcl-2. These results suggest that FCE inhibits cell proliferation and induces apoptosis via the mitochondrial-mediated intrinsic pathway. The present study demonstrates that FCE can be used as an anti-cancer agent for liver cancer based on apoptosis mechanism.

• Korean Journal of Medicinal Crop Science
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• v.16 no.3
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• pp.161-167
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• 2008

New somatic chromosome numbers and karyotype analyses of 33 medicinal herbs (30 genera, 23 families) in Korea were investigated. The chromosome numbers of 4 taxa, Euryale ferox, Rodgersia podophylla, Cirsium japonicum var. ussurience, Eehinops setifer, showed results that are different from previous reports. Among 33 taxa, 23 taxa were reported for the first time, and karyotype analyses were newly conducted for 2 taxa (Tiarella polyphylla, Crepidiastrum denticulatum) in Korea. In addition, we observed for the first time the new chromosome numbers for 4 taxa distributed evenly over the world (Lindera erythrocarpa, Corylopsis glabrescens var. gotoana, Ardisia crenata, Callicarpa japonica var. luxurians).

• The Plant Pathology Journal
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• v.16 no.6
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• pp.297-305
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• 2000

Weeds are widely grown in the field and are infected by many viruses. A survey was conducted to identify viruses infecting weeds in Korea. Virus-infected weed samples including Rorippa indica (L.) Hiern, R. islandica (Oed.) Bord, Crepidiastrum denticulatum (Houtt.) Pak & Kawanno, Achyranthes japonica (Miq.) Nakai, and Chrysanthemum boreale (Makino) Makino were collected in Kyonggi Province. These weeds were grown in the greenhouse and were isolated on 10 test plants. Several virus isolates were isolated fron infected tissues and were further studied by host range assay, serological test, electron microscopy (EM), reverse transcription-polymerase chain reaction (RT-PCR) and sequencing. Each isolated virus strain was mechanically transmitted to weeds and various hosts including Nicotiana spp., Brassica spp., Vigna unguiculata, Capsicum annuum, and Cucumis sativus and showed systemic mosaic, vein clearing, necrosis, mottle, malformation, chlorosis, and/or death of host plants in some cases. Each virus was then purified using infected leaves and observed by EM. From these results three viruses were isolated and identified as Turnip mosaic virus (TuMV), Broad bean wilt virus (BBWV), and Cucumber mosaic virus (CMV). RT-PCR using virus-specific oligonucleotide primers and the cloning were conducted to determine the nucleotide sequences of coat proteins of the three viruses their amino acid sequence were deduced. The amino acid sequence homologies were about 92.7 to 99.7%, 96.2 to 97.7%, and 93.9 to 98.6% to other reported TuMV, BBWV, and CMV strains, respectively. These results suggest that many weeds may serve as primary inoculum source of diseases caused by TuMV, BBWV, CMV and that the management of these viral diseases can be achieved through weed control.

• Korean Journal of Medicinal Crop Science
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• v.23 no.3
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• pp.214-222
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• 2015

This study was conducted to evaluate the growth conditions and vegetation of Peucedanum japonicum habitat in uninhabited islands of Incheon Korea. The emergence area of Peucedanum japonicum was 4.9 m distance in coastline, and altitude and slope was 7.4m and 47.5%. Soil pH, organic matter and $P_2O_5$ of habitat were 7.1, 8.3% and 29.0 mg/kg, and contents of K, Ca, Mg and Na were 0.66, 4.9, 3.4 and $1.13cmol^+/kg$, respectively. Growth plants with Peucedanum japonicum were total 55 taxa, and Gakeuldo in uninhabited islands was the most as 25 taxa. In growth plants with Peucedanum japonicum, plants of over 25% frequence per plot were Miscanthus sinensis, Dendranthema boreale, Lilium lancifolium, Hemerocallis middendorffii, Elymus dahuricus, Cirsium japonicum var. maackii, Asparagus schoberioides, Gypsophila oldhamiana, Cocculus trilobus, Silene aprica var. oldhamiana, Artemisia capillaris, Crepidiastrum denticulatum and Sedum oryzifolium. The vegetation of Peucedanum japonicum habitat was classified into Aster spathulifolius community, Miscanthus sinensis community, Dendranthema boreale community, Setaria viridis var. pachystachys community, Cirsium japonicum var. maackii community and Hemerocallis middendorffii community. According to general condition of habitat, suitable growing areas was determined to slightly acidic from mild alkaline soils.

• Korean Journal of Medicinal Crop Science
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• v.20 no.1
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• pp.20-26
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• 2012

This study compared the differences between the Dendranthema boreale and Dendranthema indicum in their habitat, soil adaptability, species composition and community structure in Korea. More D. boreale distributed than D. indicum to in the place where high elevation and on the surface of low degree slope. Both D. boreale and D. indicum growed well in south-east direction of the slope. The soil pH of D. boreale and D. indicum was 6.1 and 7.1, respectively. Ca, Mg, Na and organic matter content of the soil of D. boreale habitat was significantly lower than that of the D. indicum habitat. There were 102 and 88 taxa, in D. boreale and D. indicum habitat, respectively. Both species generally distributed along with herbs than along with trees. The important species found in D. boreale habitat were Artemisia princeps (57.1%) and Humulus japonicus (33.3%), and the D. indicum habitat were Miscanthus sinensis (42.9%) and Lonicera japonica (38.1%). The D. boreale group was classified into Artemisia princeps, Crepidiastrum denticulatum, Miscanthus sinensis, Humulus japonicus, Pueraria lobata, Lespedeza bicolor, Lonicera japonica and Rubus crataegifolius community. The D. indicum group was classified into Artemisia capillaris, Peucedanum japonicum, Boehmeria pannosa, Pinus thunbergii, Lonicera japonica, Quercus acutissima and Robinia pseudoacacia community. There is a large difference bewteen D. boreale and D. indicum in their habitat, soil adaptability, species composition and community structure.