• 한국미생물·생명공학회지
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• 제43권3호
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• pp.296-299
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• 2015

Cowpea mild mottle virus (CPMMV)는 국내 미보고 바이러스로, 넓은 숙주범위를 가지며, 국내 관리급 검역바이러스로 지정되어 있다. 본 연구에서는 검역현장에서 신속하게 CPMMV를 진단할 수 있는 방법을 개발하였다. 이번 연구는 사용자를 위한 검사방법 개발을 위하여, 기존의 검역현장에서 활용하는 PCR 조성과 조건을 활용하였다. 본 연구에서는 CPMMV를 특이적으로 진단할 수 있는 2개의 RT-PCR 프라이머를 개발하였으며 각각 579와 638 bp를 증폭할 수 있다. 최종적으로 증폭되는 nested PCR 산물의 크기는 (579 → 298 bp)과 (638 → 252 bp)로 CPMMV의 특이 유전자를 진단할 수 있다. 또한, 제한효소 Xho I이 반응할 수 있는 염기 서열을 삽입하여 양성대조구 플라스미드를 개발하였다. 이것은 실험실 오염으로부터 거짓양성을 검증할 수 있게 하였다. 본 연구는 CPMMV와 관련된 수입 작물로부터 검역진단에 기여할 것이라고 사료된다.

• 한국식생활문화학회지
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• 제12권5호
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• pp.561-565
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• 1997

This study was done to develop the standard recipe for nutritious cowpea starch based grape jelly. The effects of grape juice concentration (4, 8, 12%) and 0.2% calcium addition were evaluated on textural and sensory properties of grape jelly through the instrumental analysis using rheometer and sensory evaluation. Instrumental analysis showed that hardness, chewiness and gumminess of grape jelly was incerased significantly In jelly made of higher concentration of grape juice, and also in 0.2% calcium containing jelly. According to sensory evaluation, higher grape juice contains jelly showed advantages in preference of color and overall quality. Calcium addition increased sourness in sensory score. Overall, 12% grape juice and 0.2% calcium containing jelly was most preferable, which means calcium fortified cowpea starch based grape jelly was successful try for nutritious and practical food for elementary school foodservice.

• 미생물학회지
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• 제3권2호
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• pp.22-26
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• 1965

In order to investigate the host range of the mosaic disease of black locust in the Chunchon area, the sap of the mosaic-diseased leaves of black locust itself and the cowpea leaves infected with the above mentioned sap, were inoculated to 53 species of plants belong to 12 families. As to the result, no difference in infection was found as related to the virus sources, and the infection was recognized in 4 species of the family Chenopodiaceae and 8 species of the family Leguminosae. The plants recognized as hosts are as follows: the plants which showed local infection are Chenopodium album, Ch. ambrosioides, Ch. quinoa; the plants which showed systemic infection are Chenopodium amaranticolor, Phaseolus vulgaris, Robinia pseudo-acacia, Vigna sinensis; and Astragalus sinicus, Melilotus indicus, Phaseolus angularis, Pisum sativum and Vicia faba were recognized as carriers. Through investigating its host ranges and symptoms, this mosaic virus of black locust seems not to be regarded as the group of the black locust mosaic virus in southeastern Europe reported by Milinko et al (1961). And, too, it is thought hardly to exist in combination with the cowpea mosaic virus. It appears, therefore, that this mosaic virus was confined to that of black locust.

• Mycobiology
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• 제33권1호
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• pp.51-60
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• 2005

Pot greenhouse experiments were carried out to attempt to increase the salinity tolerance of one of the most popular legume of the world; cowpea; by using dual inoculation of an Am fungus Glomus clarum and a nitrogen-fixer Azospirillum brasilense. The effect of these beneficial microbes, as single- or dual inoculation-treatments, was assessed in sterilized loamy sand soil at five NaCl levels ($0.0{\sim}7.\;2ds/m$) in irrigating water. The results of this study revealed that percentage of mycorrhizal infection, plant height, dry weight, nodule number, protein content, nitrogenase and phosphatase activities, as well as nutrient elements N, P, K, Ca, Mg were significantly decreased by increasing salinity level in non-mycorrhized plants in absence of NFB. Plants inoculated with NFB showed higher nodule numbers, protein content, nitrogen concentration and nitrogenase activities than those of non-inoculated at all salinity levels. Mycorrhized plants exhibited better improvement in all measurements than that of non-mycorrhized ones at all salinity levels, especially, in the presence of NFB. The concentration of $Na^+$ was significantly accumulated in cowpea plants by rising salinity except in shoots of mycorrhizal plants which had $K^+/Na^+$ ratios higher than other treatments. This study indicated that dual inoculation with Am fungi and N-fixer Azospirillum can support both needs for N and P, excess of NaCl and will be useful in terms of soil recovery in saline area.

• 한국작물학회지
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• 제30권4호
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• pp.419-426
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• 1985

동부의 파종적기와 한계기를 구명함과 동시에 Growing Degree Days(GDD)의 이용 가능성을 검토하였던 바 시험결과를 요약하면 다음과 같다. 1. 파종기 지연에 따라 파종후 개화 및 성숙시까지의 일수가 단축되었으며 8월 14일 이후 파종은 개화하지 못했다. 2. 파종이 늦어질 수록 생육초기의 생장속도가 빨라졌고, 중원재래가 그 경향이 뚜렷하였다. 3. 5월 1일부터 7월 15일까지의 파종에서는 주경장, 주경절수, 분지수 및 경직경의 차가 없었으나 7월 30일 이후 파종부터는 파종지연 정도에 따라 급격히 감소했다. 4. 주경화경수, 분지화경수, 화경당착협수, 개체당착협수 및 100입중과 수량간에는 고도의 정의 상관관계가 있었다. 5. VITA#5는 중원재래에 비해 화경수, 개체당착협수가 않고, 100입중도 무거워 다수성이며, 두품종 공히 조파할 수록 증수의 경향이었고, 파종 한계기는 VITA#5가 6월 30일, 중원재래가 6월 15일 이었다. 6. 개화·착협 당시의 생육조건이 불량하면 협당입수가 감소된다. 7. GDD에 의한 개화까지의 표시방법은 일반 날자로 표시하는 것보다 변이계수가 작아 동부에도 이용가능성을 보였다.

• 한국작물학회지
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• 제43권4호
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• pp.245-249
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• 1998

Soybean [Glycine max (L.) Merr.), mungbean [Vigna radiata (L.) Wilcz.], cowpea [V. unguiculata (L.) Walp.], adzuki bean [V. angularis (Willd.) Ohwi & Ohashi], maize [Zea mays L.], sorghum [Sorghum bicolor (L.) Moench], sorghum $\times$ sudangrass [So bicolor intraspecific hybrid], and Japanese millet [Echinochloa crusgalli var. frumentacea (Link) W.F. Wight] were grown at two planting dates (18 June and 15 July) at Cheju in 1997 to select the best forage legumes adapted to Cheju Island for grass-legume forage rotation. Averaged across planting dates and cultivars, dry matter (DM), crude protein (CP), and total digestible nutrient (TDN) yields were 5,646, 1,056, and 3,637 kg/ha for soybean, 4,458, 676, and 2,661 kg/ha for mungbean, 3,289, 553, and 2,055 kg/ha for cowpea, 3,931, 674, and 2,489 kg/ha for adzuki bean, 12,695, 969, and 7,642 kg/ha for maize, 17,071, 1,260, and 8,857 kg/ha for sorghum, 16,355, 1,163, and 8,543 kg/ha for sorghum $\times$ sudangrass hybrid, and 8,288, 929, and 4,091 kg/ha for Japanese millet. Soybean was higher in CP, ether extract (EE), and TON content but was lower in nitrogen free extract content compared with the three other legumes. The legumes had much higher CP (13.7 to 21.9%), EE (2.42 to 6.23%), and TDN (58.7 to 69.9%) content but lower in crude fiber (CF) content (17.3 to 25.3%) than did the grasses tested except maize which had relatively lower CF content but higher TDN content. These results suggest that soybean could be the best forage legume for grass-legume forage rotation in the Cheju region.

• 한국식품과학회지
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• 제50권5호
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• pp.504-510
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• 2018

본 연구에서는 4가지 가공방법(침지, 삶음, 볶음, 가압볶음)에 의해 처리한 두류가루로 쿠키를 제조한 후 그들의 이화학적 특성과 텍스처 특성을 살펴보았다. 품질특성과 영양적 가치를 높이기 위하여 두류-찹쌀 혼합가루를 이용하여 글루텐-프리 쌀쿠키를 제조하였다. 가압볶음과 삶음에 의해 두류가루는 호화가 일어나 페이스팅 점도를 나타나지 않았으며 볶음처리에 의한 두류가루는 열처리에 의해 상호작용이 유도되어 호화온도가 증가하고 치반점도가 거의 나타나지 않은 특성을 보였다. 볶음처리한 두류가루로 제조된 쿠키는 대조군과 거의 유사한 색상을 나타냈으며 가압볶음이나 삶음 처리한 두류가루 보다는 높은 경도를 나타냈다. 녹두가루보다는 동부가루로 제조한 쿠키가 더 우수한 색상을 나타냈으며 경도도 더 높은 결과를 보였다. 결과적으로 본 연구에서 두류의 종류와 두류의 가공방법에 의해 글루텐-프리 쌀쿠키의 품질에 영향을 미치는 것을 확인할 수 있었다.

• 한국토양비료학회지
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• 제25권1호
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• pp.89-98
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• 1992

국내에서 분리(分離)된 73개의 땅콩 근류균(根瘤菌)과 31개의 도입된 Cowpea 근류균(根瘤菌)의 국내 땅콩 장려 품종(品種)에 대한 접종효과를 온실실험으로 조사하였던바 근류형성(根瘤形成)과 건물생산량 및 총(總) 질소함량(窒素含量)에서 다양한 변화(變化)를 보였다. 총(總) 건물생산량과 총(總) 질소함량(質素含量)의 서열간에 가장 높은 상관관계가 있었다. 조사된 균주중(菌株中)에서 51.9%, 26.4%, 21.7%가 각각 우량균주(優良菌株), 보통균주(普通菌株), 및 불량균주(不良菌株)로 나타났으며, JB7을 포함한 6개 균주가 가장 우수했다. 조사된 균주(菌株)는 YMA배지상에서의 생장특성에 따라 5개의 군(群)으로 분리되었다.

• 한국식품조리과학회지
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• 제21권6호통권90호
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• pp.877-887
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• 2005

This study determined the effects of soybean oil$2\%$) and chitosan($1\%$) on the quality characteristics of Omija Jelly made of various starches (mungbean starch, cowpea starch and corn starch). RVA(Rapid Visco Analyzer) viscosity was measured for starches suspended in Omija aextract with $2\%$ soybean oil and $1\%$ chitosan. The color value, syneresis, texture(rupture test and TPA test) and sensory properties of the samples were measured. Gelatinization of cowpea starch was expedited by adding soybean oil and chitosan. Otherwise, gelatinization of mungbean starch and com starch was retarded by adding chitosan. The lightness(L) and the syneresis of Omija Jelly with soybean oil and chitosan were decreased, indicating the increased transparency and stability of Omija Jelly. Rupture stress and rupture energy of Omija Jelly were decreased by adding soybean oil. Rupture stress was increased and rupture energy was decreased by adding chitosan. The addition of soybean oil improved texture of Omija Jelly, indicating that the springiness, cohesiveness and chewiness of Omija Jelly were increased and adhesiveness was decreased. By adding chitosan, the springiness and hardness of Omija Jelly were increased and the cohesiveness and adhesiveness were decreased. The overall acceptability of Omija Jelly made of $6%$ or $7\%$ cowpea starch and com starch was increased by adding soybean oil and chitosan, but the quality characteristics of Omija Jelly made of mungbean starch were not influenced by additives such as soybean oil and chitosan.

• 한국초지조사료학회지
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• 제41권3호
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• pp.198-204
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• 2021

Intercropping cereals with legumes is known to improve forage production and crude protein yield. Sorghum × sudangrass hybrids (SSH) have excellent dry matter content and high cultivation temperatures. In this study, we investigated the growth characteristics, forage productivity, and feed value of intercropping SSH with different legumes in rice paddy fields. We used five treatments in this study SSH monocropping and four intercropping treatments of SSH with, lablab, cowpea, sesbania, and two cultivars of soybean (Chookdu 1 and 2). SSH plant height was not significantly different between the monocropping and intercropping treatments. However, the plant heights of lablab, cowpea, and sesbania were significantly higher than those of the two soybean cultivars. The total dry matter yield (kg/ha) was significantly higher in SSH monocropping than in intercropping; among the intercropping treatments, the one with SSH and Chookdu 2 yielded the highest total dry matter yield. The SSH feed value was significantly different between the monocropping and intercropping treatments, although there were no differences between the intercropping treatments. Among the intercropped legumes, lablab showed the highest neutral detergent fiber and acid detergent fiber contents, and cowpea had the highest crude protein content. These results reveal that intercropping SSH with legumes in paddy fields could be a promising cultivation technique to maintain stable forage productivity.