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http://dx.doi.org/10.4014/mbl.1507.07003

Development of a Diagnostic System for the Detection of the Cowpea mild mottle virus Specific Gene in Quarantine  

Lee, Siwon (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
Lee, Jin-Young (Department of Microbiology, College of Natural Sciences, Dankook University)
Moon, Bo Yeong (Experiment & Analysis Division, Honam Regional Office, Animal and Plant Quarantine Agency)
Kim, Chang Soo (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
Shin, Yong-Gil (Experiment & Analysis Division, Incheon International Airport Regional Office, Animal and Plant Quarantine Agency)
Rho, Jae-Young (Department of Microbiology, College of Natural Sciences, Dankook University)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.3, 2015 , pp. 296-299 More about this Journal
Abstract
Cowpea mild mottle virus (CPMMV) has a wide range of hosts, such as the pea family and tomato. CPMMV is a non-reported virus in Korea, and is domestically designated as a controlled virus associated with plant quarantine. In this study, a rapid diagnostic method for the detection of CPMMV at quarantine sites was developed. For the development of a user-based system, the PCR compositions and conditions use existing methods of quarantine for the viruses. Two sets of RT-PCR and nested PCR were developed in this study that could be amplified from 579 → 298 dp and 638 → 252 bp, respectively. Furthermore, a sequence inserted positive control plasmid was developed, which is able to identify false-positives resulting from laboratory contamination. The findings of this study are important for the diagnosis of CPMMV in imported crops held in plant quarantine.
Keywords
Cowpea mild mottle virus; CPMMV; quarantine;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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1 Lee S, Kang EH, Heo NY, Kim SM, Kim YJ, Shin YG. 2013. Detection of Carnation necrotic fleck virus and Carnation ringspot virus using RT-PCR. Res. Plant Dis. 19: 36−44.   DOI
2 Lee S, Kang EH, Shin YG, Lee SH. 2013. Development of RT-PCR and nested PCR for detection of four quarantine plant viruses belonging to Nepovirus. Res. Plant Dis. 19: 220−225.   DOI
3 Lee S, Lee JY, Shin YG, Lee SH, Ahn TY. 2015. Development and verification of nested PCR assay for detection of Tobacco rattle virus in plant quarantine. J. Bacteriol. Virol. 45: 54−61.   DOI
4 Lee S, Rho JY. 2015. Development of a PCR diagnostic system for detecting Andean potato mottle virus asociated with potato quarantine in Korea. Am. J. Potato Res. In press.
5 Lee S, Shin YG. 2014. Development and practical use of RT-PCR for seed-transmitted Prune dwarf virus in quarantine. Plant Pathol. J. 30: 178−182.   DOI
6 Mink GI, Keswani CL. 1987. First report of Cowpea mild mottle virus on bean and mung bean in Tanzania. Plant Dis. 71: 557.
7 Shin YG. 2009. An advanced quarantine system for the inspection of seed-borne viruses in Korea. Ph. D. thesis. Kyungpook National University, Daegu, Gyeongsangbuk-do, Korea.
8 Stein A, Loebensten G, Koenic R. 1979. Detection of Cucumber mosaic virus and Bean yellow mosaic virus in gladiolus by enzymelinked immunosobent assay (ELISA). Plant Dis. Rep. 63: 185−188.
9 Animal, Plant and Fisheries Quarantine and Inspection Agency. 2013. List of Plant Quarantine Viruses in Korea in Newly Revised in 2013. Res. Plant Dis. 19: 67−75.
10 Brunt AA, Kenten RH. 1973. Cowpea mild mottle, a newly recognized virus infecting cowpea (Vigna unguiculata) in Ghana. Ann. Appl. Biol. 74: 67−74.   DOI
11 Brunt AA, Phillips S. 1981. “Fuzzy vein”, a disease of tomato (Lycopersicon esculentum) in western Nigeria induced by Cowpea mild mottle virus. Tropical Agriculture 58: 177−180.
12 Caruso P, Bertolini E, Ca M, López MM. 2003. A new and sensitive co-operational polymerase chain reaction for rapid detection of Ralstonia solanacearum in water. J. Microbiol. Methods 55: 257−272.   DOI
13 Jeyanandarajah P, Brunt AA. 1993. The natural occurrence, transmission, properties and possible affinities of Cowpea mild mottle virus. J. Phytopathol. 137: 148−156.   DOI
14 Lee S. 2013. A study of molecular biological detection methods for seed-transmitted viruses in quarantine. Ph. D. thesis. Dankook University, Cheonan, Chungcheongnam-do, Korea.
15 Lee S, Kang EH, Chu YM, Shin YG, Ahn TY. 2013. Development of PCR diagnosis system for plant quarantine seed-borne Wheat streak mosaic virus. Korean J. Microbiol. 49: 112−117.   DOI