• Proceedings of the Korean Society of Veterinary Clinics Conference
• /
• 2007.10a
• /
• pp.621-621
• /
• 2007

• Food Science of Animal Resources
• /
• v.25 no.1
• /
• pp.98-102
• /
• 2005

The purpose of this study was to demonstrate biochemical properties of lactoferrin (Lf) obtained from the colostrum of Korea native cow. The molecular weight of the purified Korean native cow's Lf (K-Lf) was 81kDa, the isoelectric point was 9, and the content of iron was 0.56 mg/g, which is indicated that iron saturation of the lactoferrin was 40.6%. Amino acid composition and a-helix content were different K-Lf from bovine Lf (B-Lf). Immunological cross reactivity was observed between K-Lf and B-Lf but not between K-Lf and human Lf (H-Lf) by immunodiffusion test and Western blot analysis. Out results indicate that structure of K-Lf is different from that of B-Lf although K-Lf and B-Lf were immunologically cross-reactive.

• Asian-Australasian Journal of Animal Sciences
• /
• v.27 no.7
• /
• pp.951-959
• /
• 2014

The objective of this study was to determine the effects of linseed oil or whole linseed supplementation on performance and milk fatty acid composition of lactating dairy cows. Thirty six Holstein Friesian crossbred lactating dairy cows were blocked by milking days first and then stratified random balanced for milk yields and body weight into three groups of 12 cows each. The treatments consisted of basal ration (53:47; forage:concentrate ratio, on a dry matter [DM] basis, respectively) supplemented with 300 g/d of palm oil as a positive control diet (PO), or supplemented with 300 g/d of linseed oil (LSO), or supplemented with 688 g/d of top-dressed whole linseed (WLS). All cows were received ad libitum grass silage and individually fed according to the treatments. The experiment lasted for 10 weeks including the first 2 weeks as the adjustment period, followed by 8 weeks of measurement period. The results showed that LSO and WLS supplementation had no effects on total dry matter intake, milk yield, milk composition, and live weight change; however, the animals fed WLS had higher crude protein (CP) intake than those fed PO and LSO (p<0.05). To compare with the control diet, dairy cow's diets supplemented with LSO and WLS significantly increased milk concentrations of cis-9,trans-11-conjugated linoleic acid (CLA) (p<0.05) and n-3 fatty acids (FA) (p<0.01), particularly, cis-9,12,15-C18:3, C20:5n-3 and C22:6n-3. Supplementing LSO and WLS induced a reduction of medium chain FA, especially, C12:0-C16:0 FA (p<0.05) while increasing the concentration of milk unsaturated fatty acids (UFA) (p<0.05). Milk FA proportions of n-3 FA remarkably increased whereas the ratio of n-6 to n-3 decreased in the cows supplemented with WLS as compared with those fed the control diet and LSO (p<0.01). In conclusion, supplementing dairy cows' diet based on grass silage with WLS had no effect on milk yield and milk composition; however, trans-9-C18:1, cis-9,trans-11-CLA, n-3 FA and UFA were increased while saturated FA were decreased by WLS supplementation. Therefore, it is recommended that the addition 300 g/d of oil from whole linseed should be used to lactating dairy cows' diets.

• Journal of Dairy Science and Biotechnology
• /
• v.24 no.2
• /
• pp.39-45
• /
• 2006

There has been a successful re-entry in the form of infant foods and as a product concept of "well-being" milk by feeding goat natural medicinal plants in high mountain lands and goats eating natural feeds. Typical composition of cow's milk and goat's milk are not significantly differ in major nutritional constituents. However, the noticeable differences between milks of the bovine and caprine species concern in the dimensions of the micelles, in casein composition, in size of the micelles and in the mineral charge of the micelle, but the ratio Ca/Pi in the micelle is very close for the two species The potential market in Korea could be expected to expand by keeping its freshness and nutritional benefits. The supply of goat milk products all year around is also an important to the consumers. In order to increase its market scale of goat milk, product manufacturers need extensive advertising promotion. Domestically, goat milk is currently manufactured at small scale dairy goat milk companies and consumed mainly in the form of fresh or fermented goat milk, while imported goat milk powder is used to produce infant goat milk formula by major dairy companies. Decreasing the unpleasant goaty flavour for the Korean consumers would be essential for the researchers who work for dairy science and technology.

• Korean Journal of Veterinary Service
• /
• v.23 no.4
• /
• pp.313-320
• /
• 2000

Staphylococcus aureus is a causative pathogen of bovine mastitis. It is recognized as a common pathogen in human and animal and specially enterotoxin-producing strain of S aureus is a common cause of staphylococcal food poisoning in human. Various food originated raw milk, cheese, butter produced from mastitic cow causes staphylococcal food poisoning. It is difficult to treat the staphylococcal mastitis because of increasing resistance by using overdose of antibiotics. This study was conducted to investigate the enterotoxin-production and coagulase serotypes of S aureus in Chonnam province for 6 month, 1999. Also we studied the antibiotic resistant pattern with 14 types against isolates. 18(10.1%) S aureus were isolated from 178 raw milk samples in seven farms. and 8 strains(38%) were isolated in 21 raw milk samples which was below 500,000 somatic cells. We identify that 7(87.5%) of 8 isolates and 15(83.3%) 18 isolates produce enterotoxin. Their enterotoxin serotype was type B(66.7%), type A(33.3%) and type C(13.3%). Also 2 strains of isolates was positive to the type A and B. Coagulase serotype of isolates was 2, 3, 4, 7, and 8. Most stains(70.6%) were serotype 2. And most strains(17 isolates, 94.4%) except one isolate was multiple resistant to the tested antibiotics.

• Toxicological Research
• /
• v.18 no.1
• /
• pp.99-106
• /
• 2002

The United States Environmental Protection Agency (EPA) characterized the cancer hazard of di(2-ethylhexyl)-phthalate (DEHP) as a B2 group (probable human carcinogen) and proposed "Guide-lines for Carcinogen Risk Assessment". This guidelines proposed alternative methods for analyzing carcinogen dose-response data and for extrapolating the effects of observed at high dose to predict that might occur at lower doses relevant to human exposure. This proposed guidelines state that "If in a particular case, the evidence indicated a threshold, as in the case of carcinogenicity being secondary to another toxicity that has a threshold, the margin of exposure analysis for toxicity is the same as is done for a non-cancer endpoint". DEHP is excellent candidate for reconideration under the new guidelines for carcinogen risk assessment (John Doull et al., 1998). This study is conducted about risk assessment for infant exposure on DEHP in powdered milk wing methodology in EPA's new guideline on carcinogenic risk assessment. Estimated cancer risk of DEHP in powdered milk and cow milk is 2.83$\times$$10^5$ (using cancer potency: 1.4$\times$$10^2$/ (mg/kg/day)) as mean and MOE is 12075 (using selected NOEL 20 mg/kg/day) as mean. mg/kg/day) as mean.

• Journal of Veterinary Clinics
• /
• v.8 no.1
• /
• pp.53-57
• /
• 1991

Opportunistic mycotic infection of mammary gland due to Candida tropicalis is described in a 7-year-old Holstein Friesian cow with a history of prologned use of antibiotics and corticosteroids. C. tropicalis was repeatedly isolated from the mastitic milk on simplified sunflower seed medium at 30$^{\circ}C$. The pathogen was directly detected in the infected milk by 'PHOL' technique. Microscopic examination of Gram stained smears of milk sediment revealed numerous yeast cells and hyphae morphologically consistent with Candida spp. In vitro drug sensitivity test indicated that C. tropicalis was sensitive to nystatin. Chemotherapy with intramammary infusion of nystatin was successful as evidenced by clinical and mycological observations. It emphasizes the growing significance of Candida spp. and other opportunistic fungal pathogens in Veterinary medicine.

• Microbiology and Biotechnology Letters
• /
• v.46 no.3
• /
• pp.201-209
• /
• 2018

Streptococcus thermophilus, Lactobacillus delbrueckii, Lactobacillus fermentum and Lactobacillus casei were successfully isolated from indigenous Indonesian fresh milk based on the general morphological and biochemical classification as described in Bergey's manual. Verification was conducted by sequencing of 16S rRNA after selection using the classification method mentioned in the manual. All isolates exhibited antimicrobial activity against Escherichia coli and Staphylococcus aureus in the well diffusion test. The susceptibilities of the isolated S. thermophilus 24/S1 and L. delbrueckii 94/L4 against 22 different antibiotics were determined by the disc diffusion method and variable susceptibility patterns were observed. Both isolates were susceptible to amoxicillin, the most commonly prescribed antibiotic, and resistant to sulfonamide. The presence of a plasmid was not detected after extraction. S. thermophilus 24/S1 and L. delbrueckii 94/L4 starter cultures were prepared for yogurt production after 9.5 h of incubation and the yogurt was evaluated for its flavor and quality by 30 volunteers. A score of $4.93{\pm}0.45$ out of 7 was obtained as compared to the yogurt prepared using commercial starter cultures which yielded a score of $4.76{\pm}0.30$ out of 7.

• Korean Journal of Veterinary Research
• /
• v.6 no.1
• /
• pp.37-41
• /
• 1966

Observations were made on the total serum protein values of 532 Korean cattles(341 females and 191 males and 191 males) and 90 milk cows in order to determine the effects of age, sex and nutritional status upon serum protein. Results obtained in this studies were summerized as follows; 1. The concentration of total serum protein was higher in the adult than in the younger and was generally increased with the advance of age in Korean cattle. The rate of increase in total serum protein content was more significant in the young than that of adult. 2. The average concentration of total serum protein of male did not differ significaltly from that of females in Korean cattle. 3. The concentration of total serum protein in milk cows were significaltly higher than that of Korean cattle cows and this difference seemed to be influenced by rations high in protein 4. A general tendency showed that the concentration of total serum protein was significaltly higher in the Korean cattle keeping with good nutritional status than that of poor nutritional status.

• Korean Journal of Veterinary Research
• /
• v.51 no.1
• /
• pp.1-6
• /
• 2011

Sixteen steers were used to investigate the efficacy of the sustained-release implant of bovine somatotropin (bST) in improving growth and feed:gain ratio during 12 weeks. Administration of the 400 mg bST implant resulted in a 16.1% increase in growth rate, and this increase was significant (p<.05). The use of the sustained-release implant did not alter (p>.05) feed intake and feed:gain ratio. Thirty-four cows were used to investigate the efficacy of the sustained-release implant of bST in milk production during 4 weeks. Administration of the 200 mg bST implant resulted in an 8.7% increase in milk production, and this increase was significant (p<.05). Twenty-four barrows were used to investigate the efficacy of the sustained-release implant of porcine somatotropin (pST) in improving growth, feed:gain ratio and backfat thickness during 6 weeks. Administration of the 120 mg pST implant resulted in a 11.4% increase in feed:gain ratio and a 60% decrease in backfat thickness, and these results were significant (p<.05). But the use of the sustained-release implant did not alter (p>.05) growth rate and feed intake.