Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Biochemical Properties of Lactoferrins from Korean Native Cow and Bovine Colostrum

한우와 젖소 초유로부터 분리한 Lactoferrin의 생화학적 특성

  • Yang Hee-Jin (Department of Food Biotechnology, Sungkyunkwan University) ;
  • Son Dong-Hwa (Korea Food Research Institute) ;
  • Ha Woel-Kyu (Food Research & Development Lab., Pasteur Milk Co., Ltd.) ;
  • Lee Soo-Won (Department of Food Biotechnology, Sungkyunkwan University)
  • Published : 2005.03.01
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Abstract

The purpose of this study was to demonstrate biochemical properties of lactoferrin (Lf) obtained from the colostrum of Korea native cow. The molecular weight of the purified Korean native cow's Lf (K-Lf) was 81kDa, the isoelectric point was 9, and the content of iron was 0.56 mg/g, which is indicated that iron saturation of the lactoferrin was 40.6%. Amino acid composition and a-helix content were different K-Lf from bovine Lf (B-Lf). Immunological cross reactivity was observed between K-Lf and B-Lf but not between K-Lf and human Lf (H-Lf) by immunodiffusion test and Western blot analysis. Out results indicate that structure of K-Lf is different from that of B-Lf although K-Lf and B-Lf were immunologically cross-reactive.

본 연구는 우리나라 재래종인 한우로부터 초유를 얻어 Lf을 분리·정제한 후 한우 Lf의 생화학적 특성을 확인하였다. 정제된 한우 Lf은 분자량이 81,000Da이고 등전점은 pI 9였으며, 철함량이 0.56 mg/g으로 철 포화도는 약 40.6%로 측정되었다. 한우 Lf과 젖소 Lf은 아미노산 조성과 a-helix 함량에서 서로 다르게 나타났다. 또한, 항원 항체 반응으로 확인한 결과, K-Lf과 B-Lf 간에는 교차반응을 보였으나 H-Lf과는 교차반응을 보이지 않았다.

Keywords

References

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