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Development of Single-tube Multiplex Immunocapture RT-PCR Assay for Simultaneous Detection of Two Pepper Tobamoviruses

  • Kim, Jae-Hyun;Choi, Gug-Seoun;Kim, Jeong-Soo;Lee, Sin-Ho;Choi, Jang-Kyung;Ryu, Ki-Ryun
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.164-167
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    • 2006
  • An immunocapture reverse transcription-polymerase chain reaction (IC/RT-PCR) was developed for simultaneous detection of two pepper-infecting RNA viruses, Pepper mud mottle virus (PMMoV) and Tobacco mild green mosaic virus (TMGMV). The assay could be performed in a single tube for simultaneous and sensitive detection of these tobamoviruses. This detection system revealed thousand-fold increase in detection sensitivity compare to ELISA. This method could save time and reagent cost compare to common RT-PCR which needs several reactions and several procedures of viral RNA extractions for the same number of samples.

Evaluation for Operational Efficiency of Road Management Equipment using Analytical Hierarchy Process (계층분석법을 이용한 도로관리장비 운영의 효율성 평가)

  • Yang, Choong-Heon;Kim, In-Su
    • International Journal of Highway Engineering
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    • v.14 no.5
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    • pp.157-164
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    • 2012
  • PURPOSES: Regional offices of the Ministry of Land, Transport and Maritime Affairs use a computerized system called KAMIS so as to manage road equipment systematically. Road agencies can record number of operating days by equipment, actual working hours, accumulated operating hours (or distance) by equipment, and operating cost. However, KAMIS does not provide critical information, although it is strongly related to efficient road management equipment operation. In other words, road agencies do not know whether they have sufficient equipment to handle their actual work. METHODS: Therefore, this study suggests a methodology to evaluate for operational efficiency of road management equipment using analytical hierarchy process(AHP). First of all, estimated weights related criteria can be produced by AHP, and then use operational history by pieces of equipment. RESULTS: Results show that importance of management work can differ from weather conditions through five areas. CONCLUSIONS: Commonly, this results can imply to help save money for the purchase and maintenance of road management equipment, and they would improve the functional performance of KAMIS.

A Study on the Active Balancing for High-Speed Rotors (I): Development of the Active Balancing System (고속 회전체의 능동 밸런싱에 관한 연구 (I): 능동 밸런싱 장치의 개발)

  • Kim, Jong-Soo;Park, Hyun-Kyu;Lee, Soo-Hun
    • Journal of the Korean Society for Precision Engineering
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    • v.19 no.4
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    • pp.140-146
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    • 2002
  • High speed rotating machines can be very sensitive to rotating mass unbalance that is a major source of harmful vibration for many types of rotating machinery. So, the balancing procedure is needed for all high-speed rotating system. To save the time and cost of off-line balancing, many researchers have developed the on-line balancing devices and methods. In this paper, an active balancing device, which is an electro-magnetic type, is developed and the active balancing algorithm using influence coefficient method is also proposed. The active balancing experiment for flexible rotors during operation is performed by an active balancing device. As a result, controlled unbalance responses are below the vibration limit at all rotating speed ranges with critical speed.

A research on the Automatic 3-D Blocker Design of Closed Die-Hot Forging (열간 형단조 공정의 3차원 중간 금형 자동 설계에 관한 연구)

  • Hwang, C.;Oh, S.I.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1998.03a
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    • pp.126-129
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    • 1998
  • Proper design of blocker dies is one of the most important aspect of impression and closed-die forging to achieve adequate metal distribution. Determination of the blocker configuration is a very difficult task and is art in itself, requiring skills achieved only by years of extensive experience. To save the cost and time of blocker design, many methods using computer were proposed. In this research, low pass filter method proposed by Oh etc. was applied to blocker die design of spoiler support, part of aircraft and plasticine model experiment of closed die forging of spoiler support was accomplished to verify the validity of the blocker designed.

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A Study on Weldability Criteria of Mash Seam Tailored Blank Welds in the Ultra-low Carbon Steel Applied on Automotive Body (극저탄소강의 Mash Seam TB 용접성 평가에 관한 연구)

  • 한창우;이창희;이명호
    • Journal of Welding and Joining
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    • v.20 no.4
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    • pp.538-543
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    • 2002
  • M/S TB(Mash Seam Tailored Blank) is a production method for blanks by welding together blanks of different material, thickness and coating, and is an attractive method for manufacturing car body because it makes parts lighter and can save the cost and time to manufacture. However, there have not been quantified criteria to evaluate the quality of TB weld. This study introduced FHR (failure height ratio) in order to assess formability or/and weldability of the M/S welds and the applicability of FHR was confirmed by actual auto body forming and FLD tests. Furthermore, a new parameter, HN(heat number) based on the heat input of "$Q=I^2Rt$" was proposed and assessed. It was found that the concept of HN could be utilized to evaluate the soundness of M/S welds without any destructive tests.ive tests.

Mix proportions of Concrete for Roller Compacted Concrete Dam Application (RCC 댐 콘크리트의 배합비 도출에 관한 연구)

  • 원종필;윤종환;황금식;장필성;김완영;정우성
    • Proceedings of the Korea Concrete Institute Conference
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    • 2003.05a
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    • pp.997-1002
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    • 2003
  • Roller-compacted concrete(RCC) dam is a new type that combines advantages of earthfill dam and concrete dam in construction, This method save cost due to their rapid method of construction. RCC is, used in RCC dams, no-slump concrete so it is different that measure method of consistency and mixture properties compare with conventional mass concrete, There are existing two major design method, which one used in USA the other used in Japan. The results obtained in this study would be useful in establishing mixture proportions for dam concrete for RCC dams by apply method of compound their merit.

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The Sialon Synthesis from Natural Silica and Al Powder Mixture by Using Home-style Microwave Oven (가정용 전자렌지를 사용한 천연규석분말과 Al분말 성형체로부터 사이알론상 합성에 관한 연구)

  • 안주삼
    • Journal of the Korean Ceramic Society
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    • v.34 no.1
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    • pp.1-6
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    • 1997
  • In home-style microwave oven, silica-Al powder mixture was ignited among pellets and combustion wave-front propagated to produce Si+AIN+Al2O3 as resultant phases under N2 atmosphere. Without cooling pro-cedure the resultant phases of Si+AIN+Al2O3 continously absorbed microwave and were heated to be syn-thesized into sialon phases. This synthesis rate of sialon phases from silica-Al powder mixture in home-style microwave oven was higher than that in conventional furnace, and total processing time was around 1 hour, which could save energy and cost.

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Study on the three phase dual GTO converter systems for reducing the harmonics using novel multi-steps method (새로운 멀티-스텝 방식을 적용한 3상 듀얼 GTO 콘버어터 시스템의 왜형율 개선에 관한 연구)

  • Baik, Chang-Jong;Mo, Chang-Ho;Kim, Yeong-Min;Park, Hyun-Chul;Yu, Chul-Ro
    • Proceedings of the KIEE Conference
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    • 1995.11a
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    • pp.250-254
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    • 1995
  • The proposed GTO converter system has the better harmonic characteristics, especially, in the distortion factor (55%), than the conventional 12-step converter system. The improved harmonic characteristics was achieved through the unsymmetrical angle control of the each converter. In addition, the proposed system can save the cost (20%), spase (50%) and weigh (8%). The proposed system was studied in simulation and experiment.

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A Study for CBL(Customer Baseline Load) utilization in Day Ahead Demand Response operation (상시수요응답(Day Ahead Demand Response) 운영에서의 CBL 활용방안 연구)

  • Ko, Jong-Min;Yang, Il-Kwon;Song, Jae-Ju;Jin, Sung-Il
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.58 no.1
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    • pp.28-34
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    • 2009
  • In this study firstly we survey the calculation method and the characteristics of the way of estimating CBL(Customer BaseLine Load) that is important calculation tool for DRP internationally. Also we analyze the power consumption pattern using the 15 minutes load profiles of about 120,000 customers in domestic. Based on this pattern, we provide the CBL calculation method that can be utilized in DRP to save the cost, and analyze the accuracy of the CBL calculation proposed in this paper through the simulation.

Experimental Study to measure Aerodynamical Wind Effects of KTX for Determining the Distance Between Track Centers (선로간격 설정시 KTX 열차풍 영향 측정에 관한 실험 연구)

  • Nam, Seong-Won;Kwon, Hyeok-Bin
    • 한국전산유체공학회:학술대회논문집
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    • 2008.03b
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    • pp.518-521
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    • 2008
  • When constructing a high-speed railroad, the reduction of the distance between track centers and the width of track bed will save the construction cost. However the shortening the distance between track centers may cause the stability problems due to higher wind pressure. Therefore the extensive technical review and aerodynamical study should be performed to determine the adequate distance between track centers. In this study, the impact that the increase in wind pressure due to the change of aerodynamic phenomena with the change of the distance between track centers may have on two trains passing by each other was predicted, and the stability of train operation was analyzed in order to review the distance between track centers suitable to Honam HSR trains. And we estimated the aerodynamical effects by the results of the real train experiments by using KTX.

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