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http://dx.doi.org/10.5423/PPJ.2006.22.2.164

Development of Single-tube Multiplex Immunocapture RT-PCR Assay for Simultaneous Detection of Two Pepper Tobamoviruses  

Kim, Jae-Hyun (Bio-resources, Research Group, KT&G Central Research Institute)
Choi, Gug-Seoun (National Horticultural Research Institute, Rural Development Administration)
Kim, Jeong-Soo (National Horticultural Research Institute, Rural Development Administration)
Lee, Sin-Ho (National Horticultural Research Institute, Rural Development Administration)
Choi, Jang-Kyung (Division of Biological Environment, Kanwon National University)
Ryu, Ki-Ryun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University)
Publication Information
The Plant Pathology Journal / v.22, no.2, 2006 , pp. 164-167 More about this Journal
Abstract
An immunocapture reverse transcription-polymerase chain reaction (IC/RT-PCR) was developed for simultaneous detection of two pepper-infecting RNA viruses, Pepper mud mottle virus (PMMoV) and Tobacco mild green mosaic virus (TMGMV). The assay could be performed in a single tube for simultaneous and sensitive detection of these tobamoviruses. This detection system revealed thousand-fold increase in detection sensitivity compare to ELISA. This method could save time and reagent cost compare to common RT-PCR which needs several reactions and several procedures of viral RNA extractions for the same number of samples.
Keywords
Detection; ELISA; IC/RT-PCR; pepper; PMMoV; TMGMV; Tobamovirus;
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Times Cited By KSCI : 2  (Citation Analysis)
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