• Journal of the Korean Applied Science and Technology
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• v.38 no.3
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• pp.651-661
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• 2021

Eruca sativa, called arugula, is a perennial plant in the Brassicaceae family, an edible plant commonly used in Italian cuisine. To study as a cosmetic material application E. sativa was extracted with 70% ethanol (ES). Then ES was fractionated using n-hexane, chloroform, ethyl acetate, n-butyl alcohol and water (EHex, EEA, ECHCl₃, EBuOH and EDW). EEA showed mushroom tyrosinase inhibitory activity. ES, EEA and EBuOH showed inhibition of tyrosinase activity. As a result, ES is expected to have skin whitening efficacy. ES was applied to 0.05, 0.1% the toner and emulsion formulation to test the stability. The anti-microbial activity of eight bacteria and fungi including Staphylococcus aureus and Propionibacterium acnes which cause dermatitis and acne was evaluated. EEA showed effects in all of microorganisms. The toner and emulsion containing ES with 0.05, 0.1% were passed in the challenge test. At -20, 4, 25, 55 ℃ and daylight, there was no significant change on pH, viscosity for 4 months. However, emulsions had phase separation phenomenon at 55 ℃, so the base formulation needs improvement. In addition, through the skin penetration test, EEA penetrated 0.058% in 6 hr, predicting the clinical efficacy. This means that E. sativa can contribute whitening agent and the synergistic effect of preservatives.

• Journal of the Korea Convergence Society
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• v.12 no.11
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• pp.427-434
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• 2021

Modern people express their beauty through hair coloring, but hair can be damaged by repeated chemical treatments. In order to increase the durability of dyeing and minimize the hair damage, in this study, the acidic amino acids including aspartic acid (Asp) and glutamic acid (Glu) were used to post-treat hair during hair coloring. The post-treatment with 0.75% Asp and Glu solution was carried out at room temperature for 20 minutes after dyeing bleached hair with cherry red and blue silver colors. After repeated shampooing of 1, 5, 10, 15, 20, and 25 times, L^*a^*b^* value of dyed hair was measured to confirm the dyeing durability, and the changes in tensile strength, porosity, and surface properties of the hair were also analyzed to determine the condition of the hair. In the case of cherry red and blue silver staining, the Asp and Glu experimental group showed higher color persistence than the control group, and the Asp and Glu experimental group showed higher tensile strength, lower porosity and smooth surface properties than the control group. In particular, the Asp test group showed superior color persistence and lower hair damage than the Glu test group. This study, therefore, if damaged in dyeing and bleaching in the field of hair after treatment with asp glu a combination of hair cosmetics in the development of basic data look forward to be.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.4
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• pp.317-331
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• 2021

Essential oil is a volatile substance obtained by physically obtaining fragrant plant materials made by one single plant and plant species, and is widely used for cosmetics, fragrances, and aroma therapy due to its excellent preservation, sterilization, and antibacterial effects. When essential oil would undergo the extraction and concentration processes, the agricultural chemicals thereof would be extracted and concentrated only to be harmful to the human body. This study analyzes 320 residual agricultural chemicals concentrated in the essential oil, and to this end, LC-MS/MS and GC-MS/MS are used, while the freezing process is applied instead of the conventional refining process hexane, to improve the preprocessing method. As a result of analyzing the essential oil, such ingredients as chlorpyrifos, piperonyl butoxide and silafluofen have been detected in Basil oil and Clove leaf oil. Hence, it is perceived that the residual agricultural chemicals should continue to be monitored for the essential oil.

• Korean Journal of Agricultural Science
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• v.41 no.3
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• pp.193-203
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• 2014

Natural Monument is a designated cultural property as part of the country. According to Article 2 of the Cultural Properties Protection Act, a national, ethnic and global heritage artificially or naturally formed, with a great historical, artistic, scientific and landscape significance is defined as a cultural heritage. Animals, plants, topography, geology, minerals, caves, biological products and special natural phenomena, having a great of historic, scenic and scientific value, are defined as the monument. According to Article 3 of Cultural Properties Protection Act, the conservation, management and utilization of National Heritage should be kept intact in its original form. So, Natural monuments are managing as retained its original form under the Basic Principles of current law. The highest population of coniferous tree in natural monument plant is ginkgo tree including 22 objects, followed by pines, junipers that order. And in case of broadleaf tree, there are zelkova trees, retusa fringe trees, pagoda trees, cork oaks, silver magnolias and etc. There are many of reported efficacy in available natural monument plants. The efficacy of plant species on pharmaceutical like anti-cancer, anti-diabetic, anti-obesity, antioxidant activity, neuroprotective, improves cholesterol, anti-inflammatory, liver protection and anti-bacterial efficacy, on cosmetics and beauty like the inhibiting formation of skin wrinkles, whitening effect, variety of materials and the efficacy of the proposed utilization of its various papers and etc have been widely reported. Before the Nagoya Protocol enters into force, the future role of the National Research Institute for Cultural Properties Administration of Cultural Heritage should be obtain a legal right to manage the social, cultural and national natural monument with emotional value to the plant genetic resource as a natural monument efficient ways to study and preserve traditional knowledge biological resources by securing a claim to the sovereignty of the material will be ready.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.4
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• pp.67-74
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• 2017

This study analyzed the antioxidant activities and antibacterial effects of extracts from the fruit of Citrus junos. Theextracts were obtained in both 70% ethanol solution and distilled water at a distillation temperature of $50^{\circ}C$.Three experiments were carried out between November 2016 and March 2017. The averages and the standard deviations of the results were measured. The total polyphenol and tannin contents of the ethanol extracts were $7.8{\pm}0.02mgGAE/g$ and $9.9{\pm}0.01mgTAE/g$, respectively, which were higher than the concentrations in the water extracts. Furthermore, the ethanol extracts scavenged $46.1{\pm}4.76%$ of DPPH radicals and $37.1{\pm}1.23%$ of ABTS radicals. The scavenging capability of the ethanol extracts was also higher than that of the water extracts. However, the scavenging capability of both types of extracts depended on their concentrations.All the extracts showed active antibiosis effects against every bacteria tested except for C. albicans. E. coli at 25 mg/disc showed antibiosis with $18{\pm}1.73mm$ for the water extracts, while S. epidermidis and S. aureus showed antibiosis with $17{\pm}4.36mm$ and $19{\pm}2.86mm$ for the ethanol extracts, respectively. This antibiosis rate is considerably higher. The results suggest that fruit extract from Citrus junos could be useful as a primary material for beauty or skin-related products such as soaps, shampoos, and scalp enhancers.

• The Korea Journal of Herbology
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• v.29 no.6
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• pp.63-71
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• 2014

Objectives : Lonicerae japonicae Flos(LJF) has been reported to exhibit anti-oxidant, anti-inflammatory, anti-viral, anti-rheumatoid properties. However, it is still largely unknown whether LJF inhibits the ultraviolet(UV)B-induced oxidative damage in human HaCaT keratinocytes. Therefore in this paper, we investigated the anti-oxidative capacity and protective effect of LJF against UVB-induced oxidative demage in human HaCaT keratinocytes. Methods : To evaluate the anti-oxidative activity of LJF extracts, we measured total phenolic contents, total flavonoid contents, antioxidant capacity, and superoxide scavenging activity. To give an oxidative stress to HaCaT cells, UVB was irradiated with $200mJ/cm^2$ to HaCaT cells. To detect the protective effect of LJF against UVB, we measured cell viability, DNA fragmentation and reactive oxygen species (ROS) production. In addition, we performed high-performance liquid chromatography (HPLC) analysis to find a major component of LJF. Results : LJF contained phenolic and flavonoid contents, and showed the anti-oxidant and superoxide scavenging activity. The UVB-induced oxidative conditions led to the cell death, DNA fragmentation and reactive oxygen species (ROS) production. However, pretreatment with LJF reduced oxidative conditions, including inhibition of cell death, DNA fragmentation and ROS production. In addition, we found out chlorogenic acid as major component of LJF. Conclusions : These results could suggest that LJF contained anti-oxidative contents and exhibited protective effects against UVB on human HaCaT keratinocytes. And the effective compound of LJF which could show protective activities against UVB is chlorogenic acid. Thus, LJF and chlorogenic acid would be useful for the development of drug or cosmetics treating skin troubles.

• Journal of the Korean Applied Science and Technology
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• v.38 no.5
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• pp.1209-1218
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• 2021

The aim of this study was to assure the possiblility of Achyranthes japonica Nakai for a cosmetic material. For this we examined the Anti-oxidation, Anti-inflammation, Anti-wrinkle effects of Achyranthes japonica Nakai extract. Each material is extracted from Achyranthes japonica Nakai roots(AJNR) and Achyranthes japonica Nakai stalks(AJNS) using 70% ethanol. We carried out Nitric oxide assay of the extract with cultured RAW 264.7 macrophage cells, And we conducted MMP-1 assay, Type I procollagen synthesis assay of the extract with cultured CCD-986sk fibroblast cells. After we measured ABTS+ assay and SOD assay of AJNR and AJNS, we knew that effect of anti-oxidant increased as their concentration get higher. The result of this study was that the antioxidant activity of AJNR and AJNS was similarly excellent and the anti-inflammatory activity of AJNR was significantly higher than AJNS. The MMP-1 inhibitory activity and Type I procollagen synthesis of AJNR and AJNS was generally excellent, AJNS had slightly better than AJNR. Therefore I thought it has the chance to play an imfortant role as a functional cosmetic material for anti-oxidant, anti-inflammation and wrinkle reduction effects.

• Journal of the Korean Applied Science and Technology
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• v.40 no.6
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• pp.1567-1579
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• 2023

Sucralose is used as a sucrose alternative in the food sector and is a globally approved pyrogenic, high-intensity artificial sweetener. However, due to the lack of studies on the effects of sweeteners on the brain, this study confirmed whether short-term consumption of sucralose has cognitive and memory protective effects in scopolamine-induced memory-injured animal models. After oral administration of sucralose 2, 5, and 10 mg/kg, scopolamine (1 mg/kg) was administered to the control group and the drug group 30 minutes later, and saline was administered intraperitoneally to the normal group, followed by behavioral experiments As a result of the experiment, Y-Maze, passive avoidance, and Morris WaterMaze recovered more than 10% of cognitive function compared to the control group. In addition, as a result of measuring proinflammatory cytokines, sucralose was found to inhibit IL-6 and TNF-α by more than 30%, and we observed that the expression level of ERK-CREB with intracellular signaling mechanisms increased in a concentration-dependent manner. Therefore, it suggests that sucralose is associated with functional foods for the prevention of functional food patients.

• Journal of the Korean Applied Science and Technology
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• v.34 no.1
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• pp.41-49
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• 2017

In this study, to evaluate antioxidant activity and safety on skin of Arctium lappa L. root extract, antioxidant activity was understood through total content of polyphenol, total content of flavonoid and DPPH radical scavenging activity, and cytotoxicity for B16F10 melanoma and skin cell protection effect for ultraviolet rays A were confirmed. To verify the application as cosmetic material, the first skin patch test was performed. The result of this experiment showed that as the content of Arctium lappa L. root extract increased, the content of polyphenol and flavonoid increased, and DPPH radical scavenging activity was confirmed. The result of checking cytotoxicity for B16F10 melanoma cells showed that it had low toxicity, and over 80% cell protection effect for ultraviolet rays A was confirmed. In addition, through the first skin patch test, Arctium lappa L. root extract was confirmed to have almost no skin irritation. Through this result, Arctium lappa L. root extract is excellent in skin protection from ultraviolet rays, has low toxicity for skin cells and is safe on skin, so its possibility of being a cosmetic ingredient was verified.

• Journal of Society of Preventive Korean Medicine
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• v.3 no.2
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• pp.129-150
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• 1999

This study was carried out to analyse a perception on acne for the Junior college students to provide basic data for the suitable treatment of acne to the students and the specialists. For this study, self-administering questionnaires were distributed to 480 students from 13 departments at a suburban women's junior college from May 1 to May 3, 1996. Of the students, 399 were responded and included in the analysis. The questionnaire includes the general question of objects, the question of knowledge and attitude of acne, and its treatment. The results were as follows: 1. Age distribution for the group less than 20 years was 63.7% and over 20 years was 36.3%, the group with acne was 66.2% and the group without acne was 33.8%. Proportions of acquiring the knowledge on acne was 49.6% from newspaper or magazine, 47.15 from friends, and 26.85 from TV or radio. 2. With regard to the knowledge on the presence of acne, a proportion of correctly answered for the question on the cause of acne was $3.41{\pm}1.02$ for the group with acne compared with $3.16{\pm}0.89$ for those without acne. This difference was statistically significant (p<0.05). 3. With regard to the knowledge levels on the treatment of acne, a proportion answered' skin-care center' for the question on where to select for the profer treatment was $13.21{\pm}1.88$ compared with $12.09{\pm}1.88$ for the other group. This difference was statistically significant (p〈0.05). A proportion of answered 'squeezing out acne by themselves' for the question of how to treat was $12.26{\pm}1.91$ compared with $10.83{\pm}1.25$ for the other group. 4. In the treatment according to the attitude of the group with acne, the positive group replied 'YES' in the intention of treatment by themselves was 55.9%. In conclusion, the differences in the knowledge and attitude on the presence of acne are not significant and it was found that the knowledge and attitude for the group with acne did not significantly influenced the treatment behavior. Accordingly, it is important to understand the cause of acne, provoking factors, remedy etc. For this, it is necessary to a provide an accurate information to professionals to enhance the knowledge of acne and to treat it properly.