Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Effects of sucralose on memory and cognitive function relief in a scopolamine-induced amnesia model

Scopolamine으로 인한 건망증 모델에서 sucralose의 기억력 및 인지기능 완화 효과

  • Eun-mi Jung (Department of Pharmaceutical Engineering, Daegu haany University) ;
  • Eunhong Lee (Department of Pharmaceutical Engineering, Daegu haany University) ;
  • Hyun-Ji Kwon (Advanced Translational Medicine, Konkuk University) ;
  • Jihye Lee (Daegu Haany University Industry Academic Cooperation Foundation) ;
  • Hye-jeong Kim (Department of Pharmaceutical Engineering, Daegu haany University) ;
  • Jinhan Park (Department of K-Beauty Business, College of Cosmetics and Pharm, Daegu Haany University) ;
  • Jongwon Lee (Department of Biochemistry, School of Medicine, Daegu Catholic University) ;
  • Ji Wook Jung (Department of Pharmaceutical Engineering, Daegu haany University)
  • 정은미 (대구한의대학교 일반대학원 제약공학과) ;
  • 이은홍 (대구한의대학교 일반대학원 제약공학과) ;
  • 권현지 (건국대학교 일반대학교원 첨단중개의학과) ;
  • 이지혜 (대구한의대학교 산학협력단) ;
  • 김혜정 (대구한의대학교 일반대학원 제약공학과) ;
  • 박진한 (대구한의대학교 화장품제약대학 K-뷰티학부) ;
  • 이종원 (대구가톨릭대학교 의과대학 생화학교실) ;
  • 정지욱 (대구한의대학교 일반대학원 제약공학과)
  • Received : 2023.12.27
  • Accepted : 2023.12.29
  • Published : 2023.12.30
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Abstract

Sucralose is used as a sucrose alternative in the food sector and is a globally approved pyrogenic, high-intensity artificial sweetener. However, due to the lack of studies on the effects of sweeteners on the brain, this study confirmed whether short-term consumption of sucralose has cognitive and memory protective effects in scopolamine-induced memory-injured animal models. After oral administration of sucralose 2, 5, and 10 mg/kg, scopolamine (1 mg/kg) was administered to the control group and the drug group 30 minutes later, and saline was administered intraperitoneally to the normal group, followed by behavioral experiments As a result of the experiment, Y-Maze, passive avoidance, and Morris WaterMaze recovered more than 10% of cognitive function compared to the control group. In addition, as a result of measuring proinflammatory cytokines, sucralose was found to inhibit IL-6 and TNF-α by more than 30%, and we observed that the expression level of ERK-CREB with intracellular signaling mechanisms increased in a concentration-dependent manner. Therefore, it suggests that sucralose is associated with functional foods for the prevention of functional food patients.

Sucralose는 식품 분야에서 sucrose 대체물로 사용되며 세계적으로 승인된 무열량 고강도 인공 감미료이다. 하지만 감미료가 뇌에 미치는 영향에 대한 조사는 미비하여 본 연구에서 scopolamine으로 유발된 기억력 손상 동물모델에서 sucralose 단기 섭취가 인지 및 기억 보호 효과를 가지는지 확인하였다. Sucralose 2, 5, 10 mg/kg를 경구투여하였으며 30분 후 대조군, 약물군에 scopolamine (1mg/kg) 복강투여를 진행하였고 정상군에는 식염수로 복강투여 후 행동실험을 수행하였다. 결과적으로 Y-maze, passive avoidance, Morris water maze에서 대조군과 비교해 10% 이상의 인지기능이 회복되는 결과를 도출하였다. 또한, 전염증성 사이토카인을 측정한 결과 sucralose가 IL-6와 TNF-α를 30% 이상 억제하는 것을 보였으며 세포 내 신호 메커니즘으로 ERK-CREB의 발현량을 관찰한 결과 농도 의존적으로 증가하였다. 따라서 sucralose는 건망증 환자의 예방과 치료를 위한 기능성 식품과 관련이 있다고 시사된다.

Keywords

Acknowledgement

본 과제(결과물)는 교육부와 한국연구재단의 재원으로 지원을 받아 수행된 3단계 산학연합력 선도대학 육성사업(LINC 3.0)의 연구 결과이며, 2022년도 정부(과학기술정보통신부)의 재원으로 한국연구재단 - 이공분야기초연구사업의 지원을 받아 수행된 연구임(No. NRF-022R1F1A1074902).

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