• 한국미생물·생명공학회지
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• 제49권2호
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• pp.167-173
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• 2021

Reactive oxygen species (ROS) disrupt the cellular redox balance, exert cytotoxic effects, and consequently promote the development of various diseases in humans. Previous studies have reported that antioxidants counteract the adverse effects of ROS. Several studies examine the whitening effects of various agents based on their ability to inhibit tyrosinase activity. Tyrosinase is a critical enzyme involved in the synthesis of melanin, which protects the skin against radiation. Various agents exhibiting antioxidant and tyrosinase inhibitory activities have been synthesized. However, these synthetic drugs are associated with toxicity, decreased safety, and poor skin penetration in vivo, which has limited the clinical application of synthetic drugs. This study examined the antioxidant and tyrosinase inhibitory activities of some microalgae. The methanol, dichloromethane, and ethyl acetate extracts of four microalgal species (Tetraselmis tetrathele, Dunaliella tertiolecta, Platymonas sp., and Chaetoceros simplex) were prepared. The physiological and whitening effects of microalgal extracts were investigated by measuring the antioxidant and tyrosinase inhibitory activities. The ethyl acetate extract of D. tertiolecta exhibited the highest antioxidant and tyrosinase inhibitory activities. Future studies must focus on examining the whitening effects of microalgae on cell lines to facilitate the development of microalga-based therapeutics for skin diseases, functional health foods, and whitening agents. Thus, microalgae have potential applications in the pharmaceutical, food, and cosmetic industries.

• 한국염색가공학회지
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• 제31권4호
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• pp.323-331
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• 2019

Sorghum is a rich source in phytochemicals, such as tannins, phenolic acids, anthocyanins, phytosterols and policosanols. Sorghum has been known to have antimicrobial, antiinflammatory, antioxidant properties. In this study, poly(vinyl alcohol)(PVA)-sorghum extract(SE) composite nanoweb was produced by electrospinning and its characteristics including bioactivities were investigated. The SE had antimicrobial and antiinflammatory activities as well as a reduced cytotoxicity. The PVA-SE nanoweb had a highly enhanced antimicrobial activity compared to PVA nanoweb. The amount of proinflammatory cytokine released from macrophages treated with the PVA-SE nanoweb was reduced. The PVA-SE nanoweb can be a potential candidate for medical and cosmeceutical materials providing antimicrobial and antiinflammatory activities with a low degree of cytotoxicity.

• 생명과학회지
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• 제24권6호
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• pp.648-654
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• 2014

포도는 전 세계적으로 가장 많이 재배되는 과수 중의 하나로 포도 및 포도 가공제품에 대한 성분분석, 항산화, 항암 등 다양한 생물학적 효능 연구가 이루어지고 있으나 겨울처 전정되어 버려지는 전정가지에 대한 연구는 미미한 실정이다. 본 연구에서는 포도전정가지 추출물(GPSE)의 총 폴리페놀함량, 항산화활성, 미백 및 주름개선효과를 검증하였다. 먼저 HPLC분석에는 GPSE는 614.05 ug/g의 trans-resveratrol을 함유하고 있는 것으로 관찰되었다. DPPH 라디칼소거능, ABTS 라디칼소거능 등의 항산화 실험을 통해서 대조군으로 사용된 BHA와 L-ascrobic acid에 비교하여 GPSE은 높은 항산화 활성을 나타내었다. 미백활성 측정을 위한 tyrosinase 저해활성 실험에서도 GPSE는 15.35%, arbutin은 1.48%로 GPSE의 미백효과가 높게 나타났으며, 주름개선 실험에서는 대조군과 비슷한 효과를 가지고 있는 것을 확인 하였다. 이와 같은 결과로 미루어 보아 GPSE는 trans-resveratrol 함량이 높은 천연항산화제로 기능성 화장품 및 식품으로의 가능성을 확인하였다.

• 한국미생물·생명공학회지
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• 제40권4호
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• pp.364-370
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• 2012

본 연구는 도화를 70% 아세톤 추출하여 sephadex LH-20으로 분리한 후 8개의 분획물을 얻을 수 있었다. 미백효과 측정으로 tyrosinase 저해활성을 측정한 결과 분획물 8의 경우 1,000 ppm에서 92.2%를 나타내었으며, 멜라노마 세포에 의한 tyrosinase 저해활성은 100 ppm에서 63.4%, 멜라닌 생합성은 저해능은 100 ppm에서 71.7%의 효과를 나타내어 분획물 8의 효능이 가장 우수하였다. 주름개선 효과 측정으로 elastase 저해활성을 측정한 결과 분획물 5, 7이 1,000 ppm에서 각각 71.4, 74.5%의 저해활성을 나타내었으며, collagenase 저해활성과 collagen 생합성량을 측정한 결과 분획물 8이 100 ppm에서 80.0% 이상의 저해활성을 나타내었다. 이상의 결과로 미루어 보아 도화 분획물의 미백 및 주름개선 효과가 있음을 확인할 수 있었고, 기능성 화장품 소재로서의 가능성을 확인할 수 있었다.

• 한국수산과학회지
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• 제53권3호
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• pp.297-307
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• 2020

This study investigated the cosmetic effects of enzymatic hydrolytes of an aquatic by-product, fish skin. The skins of olive flounder Paralichthys olivaceus (PO) and Alaska pollock Gadus chalcogrammus (AP) were hydrolyzed using pepsin, Alcalase, and Protemax. Three enzymatic hydrolytes were obtained and the inhibitory effects of these hydrolytes on the aging-related enzymes tyrosinase, elastase, and collagenase were determined. The results indicated that the pepsin hydrolytes of PO and PA had stronger activities than the other hydrolytes. PO and PA also significantly reduced the intracellular reactive oxygen species levels in and improved the viability of H₂O₂-treated Vero cells; decreased nitric oxide production by and increased the cell viability of lipopolysaccharide-treated RAW 264.7 cells; and reduced intracellular reactive oxygen species levels and improved the viability of ultraviolet B irradiated HaCaT cells and human dermal fibroblasts. Furthermore, PO and PA remarkably reduced the intra- and extracellular melanin contents of alpha-melanocyte-stimulating hormone-stimulated B16F10 cells. These results demonstrate that PO and PA have potential for use in the cosmetic industry.

• 대한본초학회지
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• 제27권5호
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• pp.55-63
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• 2012

Objectives : The solvent extracts of Pruni persicae Flos were investigated for the activities of anti-oxidant and anti-inflammatory effects to apply as a functional ingredient for cosmetic products. Methods : In this study, the fractions of P. persicae Flos were extracted with 70.0% acetone and purified using Sephadex LH-20 column chromatography. As a result, eight fractions were isolated. We performed MTT assay, total polyphenol contents, DPPH free radical scavenging assay, SOD-like activity, xanthine oxidase inhibition assay, astringent activity assay, hyaluronidase inhibition assay and the production of nitric oxide. Results : For anti-oxidant effects, the electron donating ability of fraction (Fr.) 2-5, Fr.-8 isolated from P. persicae Flos was above 90.0% at 100 ppm respectively. The superoxide dismutase (SOD) - like activity of Fr.-5 isolated from P. persicae Flos was 92.1% at 1,000 ppm. The xanthine oxidase inhibitory effect of Fr.-6 isolated from P. persicae Flos was about 83.3% at 1,000 ppm. Hyaluronidase inhibition activity related to the anti-inflammation effect was 94.0% for Fr.-4 isolated from P. persicae Flos at 500 ppm. In the anti-inflammation effect, the Fr.-4 isolated from P. persicae Flos inhibited the generation of nitric oxide. Conclusions : All these findings suggested that the fractions of P. persicae Flos has a great potential as a cosmeceutical ingredient with a anti-oxidant and anti-inflammatory effects.

• Journal of Applied Biological Chemistry
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• 제54권2호
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• pp.89-93
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• 2011

오이풀 아세톤 추출물로부터 용매분획물의 기능성 화장품 소재로 활용하기 위하여 항산화 효과를 측정한 후 화장품 소재로서의 가능성을 검증하였다. 항산화 효과를 확인하기 위하여 electron donating ability을 측정한 결과 ethyl actate 층과 n-butyl alcohol 층이 전 농도에서 대조군 BHA와 유사한 활성을 나타내었으며, ABTS radical cation decolorization assay 결과 ethyl acetate 층, n-butyl alcohol 층, water 층 모두 전 농도에서 99% 이상의 효과를 나타내어 BHA와 유사하였다. hydrogen peroxide scavenging assay 결과 ethyl acetate 층, n-butyl alcohol 층이 대조군 ascorbic acid 보다 효과가 높았다. Superoxide dismutase (SOD) 유사 활성 측정 결과, n-butyl alcohol 층이 1,000 ${\mu}g/mL$ 농도에서 50% 이상의 효능이 있었으며, Superoxide anion 라디칼 소거능은 n-butyl alcohol 층의 1,000 ${\mu}g/mL$에서 45%의 효과가 있었다. 이상의 결과로 미루어 보아 오이풀 아세톤 추출물의 분획물 중 ethyl acetate 층과 n-butyl alcohol 층에서 항산화 효과가 있었으며, 새로운 항산화 화장품의 소재로서 가능성을 확인 할 수 있었다.

• Fisheries and Aquatic Sciences
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• 제23권3호
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• pp.6.1-6.9
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• 2020

Background: Halophyte plant (HPs), a salt-resistant flora, has been reported to provide several health benefits, but the knowledge of its cosmeceutical potential is still ambiguous. Here, 70% ethanol extracts of 22 HPs collected from along the coast of South Korea were investigated for their potentials of antioxidant, anti-aging, and whitening properties for use as materials in novel cosmeceuticals. Methods: Antioxidant activities were determined by DPPH (1,1-diphenyl-2-pricrylhydrazyl) free radical and hydrogen peroxide scavenging assays, and skin aging-related enzyme activities (anti-elastase, anti-collagenase, anti-hyaluronidase, and anti-tyrosinase) were evaluated by using the spectrophotometric method. Results: Among the 22 HPs, we found that Ischaemum antephoroides f. coreana and Atriplex gmelinii extracts presented the strongest scavenging effects against DPPH free radical and hydrogen peroxide, respectively. Our finding additionally suggested that Salicornia europaea extract might provide a major source of anti-elastase and anti-hyaluronidase; meanwhile, Rosa rugosa extract showed the highest anti-collagenase effect. Furthermore, the highest tyrosinase inhibitory activity was possessed by Spartina anglica extract. Conclusion: These findings may suggest that halophyte plants showing biological activities may be potent inhibitors of tyrosinase, elastase, collagenase, and hyaluronidase and could be useful for application in cosmeceuticals.

• 동의생리병리학회지
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• 제31권3호
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• pp.188-193
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• 2017

This study investigated the antioxidant activities of distilled water, ethanol and methanol extracts of Plunus mume flower buds (PFB). The various solvent extracts of PFB were evaluated for their total polyphenol, flavonoid, reducing power and free radical scavenging activities by FRAP and DPPH analysis. The ethanol extract of PFB contained significantly higher amounts of total polyphenols (145 mg GAE/g) and flavonoids (25.43 mg QE/g) than methanol (132 and 25.42) and distilled water (113.6 and 18.04). Among solvent extracts of PFB, the ethanol extract showed the highest antioxidant activities. The 100% ethanol extract of PFB contained significantly higher amounts of total polyphenols and flavonoids than 70% and 50% ethanol extracts. Moreover, the 100% ethanol extract of PFB showed high efficacy in DPPH radical scavenging activity and in collagenase inhibition activity. This results suggest that 100% ethanol extract of PFB has the most effective antioxidant capacity compared to the methanol and water extracts tested in the present study. Thus, it can be applied for the effective extraction of functional material from PFB for usage of cosmeceutical and/or food industries.

• 한국산림과학회지
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• 제97권3호
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• pp.215-220
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• 2008

최근 식물을 이용한 추출물이 화장품과 의약품에 많이 이용되고 있다. 이에 본 연구에서는 수목의 미활용 부위인 리기다소나무 내수피의 화장품소재로써 가능성을 검토하고자 연구하였다. 이를 위해 리기다소나무 내수피 추출물을 이용하여 아질산염 소거능, 항염효과, 항균효과를 분석하여 다음과 같은 결론을 얻었다. 아질산염 소거능 실험결과 100ppm에서 물가용부는 88.7%, EtOAc가용부는 99%로 대조군인 vitamin C와 유사한 소거작용을 나타내어 리기다소나무 내수피 추출물의 뛰어난 아질산염 소거능을 확인할 수 있었다. 시료의 NO 저해활성이 세포독성으로 인한 cell population의 저하에서 기인하는 것인지 관찰하기위해 MTT assay를 통해 cell의 생존율을 측정한 결과 80% 이상의 세포 생존율을 나타내어 이것을 토대로 NO 저해활성의 실험을 진행하였다. LPS로 활성화된 Raw 264.7 세포의 배양액 중에 생성된 nitrite양을 griess시약을 사용하여 NO생성 저해를 측정하였다. 물가용부와 EtOAc가용부 모든 농도에서 12시간부터 24시간까지 NO생성을 저해하였으며, 물가용부는 100ppm의 낮은 농도에서 뛰어난 NO생성 저해능을 나타내었다. 항균효과 측정 실험에서 그람양성균인 S. aureus에 대하여 물가용부 3mg/disc에서 14mm의 생육 저해환을 나타내었으며, EtOAc가용부는 3mg/disc에서 15mm의 생육 저해환을 나타내었다. 효모인 C. albicans에 대해서는 EtOAc가용부 3mg/disc에서 15mm의 생육 저해환을 나타내었으며, S. epidermidis와 그람음성균인 E. coli에서는 생육 저해환을 나타내지 않았다. 본 연구의 결과에서는 리기다소나무 내수피 추출물의 항염 및 항균효과와 천연 소재로서 화장품 산업에 활용 가능성을 확인할 수 있었다.