• Title/Summary/Keyword: Core Center

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Molecular and Functional Characterization of Monocot-specific Pex5p Splicing Variants, Using OsPex5pL and OsPex5pS from Rice (Oryza sativa)

  • Lee, Jung Ro;Jung, Ji Hyun;Kang, Jae Sook;Kim, Jong Cheol;Jung, In Jung;Seok, Min Sook;Kim, Ji Hye;Kim, Woe Yeon;Kim, Min Gab;Kim, Jae-Yean;Lim, Chae Oh;Lee, Kyun Oh;Lee, Sang Yeol
    • Molecules and Cells
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    • v.23 no.2
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    • pp.161-169
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    • 2007
  • We identified two alternatively spliced variants of the peroxisomal targeting signal 1 (PTS1) receptor protein Pex5ps in monocot (rice, wheat, and barley) but not in dicot (Arabidopsis and tobacco) plants. We characterized the molecular and functional differences between the rice (Oryza sativa) Pex5 splicing variants OsPex5pL and OsPex5pS. There is only a single-copy of OsPEX5 in the rice genome and RT-PCR analysis points to alternative splicing of the transcripts. Putative light-responsive cis-elements were identified in the 5' region flanking OsPEX5L and Northern blot analysis demonstrated that this region affected light-dependent expression of OsPEX5 transcription. Using the pex5-deficient yeast mutant Scpex5, we showed that OsPex5pL and OsPex5pS are able to restore translocation of a model PTS1 protein (GFP-SKL) into peroxisomes. OsPex5pL and OsPex5pS formed homo-complexes via specific interaction domains, and interacted with each other and OsPex14p to form hetero-complexes. Although overexpression of OsPex5pL in the Arabidopsis pex5 mutant (Atpex5) rescued the mutant phenotype, overexpression of OsPex5pS only resulted in partial recovery.

A Modified Current Differential Relaying Algorithm for Transformer Protection Considered by a Remanent Flux (잔류자속을 고려한 변압기 보호용 수정 전류차동 계전방식)

  • Kang, Y.C.;Jin, E.S.;Won, S.H.;Lim, U.J.;Kang, S.H.
    • Proceedings of the KIEE Conference
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    • 2003.11a
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    • pp.262-265
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    • 2003
  • During magnetic inrush or over-excitation saturation of the core in a transformer draws a large exciting current. This can cause mal-operation of a differential relay. This paper proposes a modified current differential relay for transformer protection. In order to cope with the remanent flux at the beginning. the start of saturation of the core is detected and the core flux at the instant is estimated by inserting the differential current into a magnetization curve. Then, this core flux value can be used to calculate the core flux. The proposed relay calculates the core-loss current from the induced voltage and the core-loss resistance; the relay calculates the magnetizing current from the core flux and the magnetization curve. Finally, the relay obtains the modified differential current by subtracting the core-loss current and the magnetizing current from the conventional differential current. The proposed technique not only discriminates magnetic inrush and over-excitation from an internal fault, but also improves the speed of the conventional relay.

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TC1 (C8orf4) is involved in ERK1/2 pathway-regulated G1- to S-phase transition

  • Wang, Yi-Dong;Bian, Guo-Hui;Lv, Xiao-Yan;Zheng, Rong;Sun, Huan;Zhang, Zheng;Chen, Ye;Li, Qin-Wei;Xiao, Yan;Yang, Qiu-Tan;Ai, Jian-Zhong;Wei, Yu-Quan;Zhou, Qin
    • BMB Reports
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    • v.41 no.10
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    • pp.733-738
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    • 2008
  • Although previous studies have implicated a role for TC1 (C8orf4) in cancer cell proliferation, the molecular mechanism of its action is still largely unclear. In this study, we showed, for the first time, that the mRNA levels of TC1 were upregulated by mitogens (FBS/thrombin) and at least partially, through the ERK1/2 signaling pathway. Interestingly, the over-expression of TC1 promoted the $G_1$- to S-phase transition of the cell cycle, which was delayed by the deficiency of ERK1/2 signaling in fibroblast cells. Furthermore, the luciferase reporter assay indicated that the over-expression of TC1 significantly increased Cyclin D1 promoter-driven luciferase activity. Taken together, our findings revealed that TC1 was involved in the mitogen-activated ERK1/2 signaling pathway and positively regulated $G_1$- to S-phase transition of the cell cycle. Our results may provide a novel mechanism of the role of TC1 in the regulation of cell proliferation.

Distributed Hierarchical Location Placement of Core Nodes in the OCBT Multicast Protocol (OCBT 멀티캐스트 프로토콜에서 core 노드의 분산 계층 위치 결정)

  • 황경호;조동호
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.1A
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    • pp.90-95
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    • 2000
  • In the Ordered Core Based Tree(OCBT) protocol, a core location is the most important feature to affect the performance. In this paper, the location placement of multiple level cores is studied. The proposed algorithm isthat each node in the network evaluates a sum of shortest path costs from all the other nodes and the entirenetwork is divided into a hierarchy region to have 3-logical level(Small, Medium, Large). The node to have thelowest cost in each S-Region is decided to be a core node. Then, the core nodes in the each S-Region evaluatea sum of shortest path costs from all the other core nodes in the same M-Region. The core node to have thelowest cost is decided to be the upper level core node. Similarly the highest level core node is decided in theL-Region. The proposed algoritthm is compared with conventional two methods to put the core nodes in thenetwork One is the random method to put the core nodes randomly. The other is the center method to locatethe core node at the nearest node from the center of each S-Region and then to locate the highest level corenode at the nearest core node from the center of the entire network. Extensive simulations are performed in theview of mean tree cost and join latency. Simulation results show that the proposed algorithm has betterperformance than random method or center method.

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