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Improvement of Reproductive Efficiency of Artificial Insemination Following Estrus Induction in Dog II. Semen Freezing and Artificial Insemination in Dog

  • Lee, Y. R.;S. Y. Choe
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.92-92
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    • 2002
  • Considerable attention has been focused on the cryopreservation of semen and estrus induction in dog, as consequence of poor productivity caused by long anestrus period, in order to enhance the productivity of youngs and to preserve the breeds. The objectives of this study were to evaluate semen quality after cryopreservation and to evaluate the Pregnancy rate after insemination (AI). Fifty infertilie dogs (age 2∼3 years) were selected for the study and divided into three different estrus induction treatment groups. Group 1: dogs (n=15) were given clomifene (0.1 mg/kg) orally for five days at 12 hr intervals. Group 2: dogs (n=15) were given bromocriptine (50 $\mu\textrm{g}$/kg) orally for five days at 12 hr intervals, followed by single injection intravenously of 500 IU GnRH (Group 3, n=20) when pro-estrus occurred. The rates of pregnancy in estrus inducted dogs mated naturally compared to those inseminated artificially with ejaculated fresh semen and frozen-thawed semen. Estrus detection was performed using the method of vaginal smear and confirmed by the plasma progesterone assay. The ejaculated semen to freeze was exposed to a mixture of Tris extender with cryoprotectant (Trisma, 81 mM: TES, 209 mM: citric acid, 6 mM; glucose, 5 mM; glycerol, 8%) and cryopreserved gradually by slow-cooling at 17 cm above the surface of liquid nitrogen (LN$_2$) for 23 min. The motility of frozen-thawed spermatozoa was assessed by phase-contrast microscopy. To assess their viability and acrosome content, spermatozoa were stained with a vital stain and Fluorescence conjugated lectin Pisum Savitum Agglutinin (FITC/PAS), respectively. Pregnancy was confirmed by ultrasonograpy on day 25, 35 and 55 post insemination. The use of fresh semen, the pregnancy rates were observed 66.6, 66.6, 75.0 and 83.3% in natural estrus, clomifene induced, bromocriptine induced and a combination of GnRH and bromocriptine, respectively. The use of frozen-thawed semen, the pregnancy rates were observed 66.6, 33.3, 50.0 and 60.0% in natural estrus, clomifene induced, bromocriptine induced and a combination of GnRH and bromocriptine, respectively. No difference was observed in the number of offspring produced among natural estrus and treated groups inseminated with fresh or frozen-thawed semen. In conclusion, the pregnancy rate of dogs treated with a combination of GnRH and bromocriptine was more effective than use of clomifene or bromocriptine only. In addition, frozen-thawed semen can be used successfully far artificial insemination in dog.

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Genetic Environments at the Ssangjeon Tungsten-bearing Hydrothermal Vein Deposit (쌍전 함 텅스텐 열수 맥상광상의 생성환경)

  • Sunjin Lee;Sang-Hoon Choi
    • Economic and Environmental Geology
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    • v.55 no.6
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    • pp.689-699
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    • 2022
  • The Ssangjeon tungsten deposit is located within the Yeongnam Massif. Within the area a number of hydrothermal quartz veins were formed by narrow open-space filling of parallel and subparallel fractures in the metasedimentary rocks as Wonnam formation, Buncheon granite gneiss, amphibolite and/or pegmatite. Mineral paragenesis can be divided into two stages (stage I, ore-bearing quartz vein; stage II, barren quartz vein) by major tectonic fracturing. Stage I, at which the precipitation of major ore minerals occurred, is further divided into three substages (early, middle and late) with paragenetic time based on minor fractures and discernible mineral assemblages: early, marked by deposition of arsenopyrite with pyrite; middle, characterized by introduction of wolframite and scheelite with Ti-Fe-bearing oxides and base-metal sulfides; late, marked by Bi-sulfides. Fluid inclusion data show that stage I ore mineralization was deposited between initial high temperatures (≥370℃) and later lower temperatures (≈170℃) from H2O-CO2-NaCl fluids with salinities between 18.5 to 0.2 equiv. wt. % NaCl of Ssangjeon hydrothermal system. The relationship between salinity and homogenization temperature indicates a complex history of boiling, fluid unmixing (CO2 effervescence), cooling and dilution via influx of cooler, more dilute meteoric waters over the temperature range ≥370℃ to ≈170℃. Changes in stage I vein mineralogy reflect decreasing temperature and fugacity of sulfur by evolution of the Ssangjeon hydrothermal system with increasing paragenetic time.

Numerical Simulation of Standing Column Well Ground Heat Pump System Part II: Parametric Study for Evaluation of the Performance of Standing Column Well (단일심정 지열히트펌프의 수치적 모델링 Part II: 단일심정 지열히트펌프의 성능평가를 위한 매개변수 연구)

  • Park, Du-Hee;Kim, Kwang-Kyun;Kwak, Dong-Yeop;Chang, Jae-Hoon;Na, Sang-Min
    • Journal of the Korean Geotechnical Society
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    • v.26 no.2
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    • pp.45-54
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    • 2010
  • The SCW numerical model described in the companion paper was used to carry out a comprehensive parametric study to evaluate the performance of the SCW. The five ground related parameters, which are porosity, hydraulic conductivity, thermal conductivity, specific heat, geothermal gradient, and five SCW design parameters, which are pumping rate, well depth, well diameter, dip tube diameter, bleeding rate, were used in the study. Two types of numerical simulations were performed. The first type was used to perform short-term (24-hour) simulation, while the second type 14 day simulation. The study results indicate that the parameters that have important influence on the performance of SCW were hydraulic conductivity, thermal conductivity, geothermal gradient, pumping rate, and bleeding rate. The thermal conductivity had the most important influence on the performance of the SCW. With the increase in the geothermal gradient, the performance increased in the heat mode, but decreased in the cooling mode. The hydraulic conductivity influenced the performance when the value was larger than $10^{-4}m/s$. The depth of the well increased the performance, but at the cost of increased cost of boring. The bleeding had an important influence on SCW, greatly enhancing the performance at a limited increased cost of operation. Overall, this study showed that various factors had a cumulative influence on the performance of the SCW, and a numerical simulation can be used to accurately predict the performance of the SCW.

Effect of Deposition and Heat Treatment Conditions on the Electrical and Optical Properties of AZO/Cu/AZO Thin Film (증착 및 열처리 조건에 따른 AZO/Cu/AZO 박막의 전기적·광학적 특성 평가)

  • Chan-Young Kim;Ha-Eun Lim;Gaeun Yang;Sukjeang Kwon;Chan-Hee Kang;Sang-Chul Lim;Taek Yeong Lee
    • Korean Journal of Materials Research
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    • v.33 no.4
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    • pp.142-150
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    • 2023
  • AZO/Cu/AZO thin films were deposited on glass by RF magnetron sputtering. The specimens showed the preferred orientation of (0002) AZO and (111) Cu. The Cu crystal sizes increased from about 3.7 nm to about 8.5 nm with increasing Cu thickness, and from about 6.3 nm to about 9.5 nm with increasing heat treatment temperatures. The sizes of AZO crystals were almost independent of the Cu thickness, and increased slightly with heat treatment temperature. The residual stress of AZO after heat treatment also increased compressively from -4.6 GPa to -5.6 GPa with increasing heat treatment temperature. The increase in crystal size resulted from grain growth, and the increase in stress resulted from the decrease in defects that accompanied grain growth, and the thermal stress during cooling from heat treatment temperature to room temperature. From the PL spectra, the decrease in defects during heat treatment resulted in the increased intensity. The electrical resistivities of the 4 nm Cu film were 5.9×10-4 Ω·cm and about 1.0×10-4 Ω·cm for thicker Cu films. The resistivity decreased as the temperature of heat treatment increased. As the Cu thickness increased, an increase in carrier concentration resulted, as the fraction of AZO/Cu/AZO metal film increased. And the increase in carrier concentration with increasing heat treatment temperature might result from the diffusion of Cu ions into AZO. Transmittance decreased with increasing Cu thicknesses, and reached a maximum near the 500 nm wavelength after being heat treated at 200 ℃.

SNU 1.5MV Van de Graaff Accelerator (IV) -Fabrication and Aberration Analysis of Magnetic Quadrupole Lens- (SNU 1.5MV 반데그라프 가속기 (IV) -자기 4극 렌즈의 제작과 수차의 분석-)

  • Bak, H.I.;Choi, B.H.;Choi, H.D.
    • Nuclear Engineering and Technology
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    • v.18 no.1
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    • pp.1-8
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    • 1986
  • A magnetic quadrupole doublet was fabricated for use at the pre-target position of SNU 1.5MV Van de Graaff accelerator and then its optical characteristics were measured and analysed. The physical dimensions are: pole length 180mm, aperture radius 25mm, pole tip radius 28.75mm. Material for poles and return yokes is carbon steel KS-SM40C. Coils have 480 turns per one pole and air-cooling is adopted. Applying the d.c. current 2.99$\pm$0.03A to the lens, and using the Hall probe, magnetic field elements $B_{\theta}$ , $B_{\gamma}$, were measured at the selected Points along each coordinate direction r,$\theta$, z. From the area integration and orthogonal polynomial fitting for the measured data, the magnetic Field gradient G=566.3$\pm$2.1 gauss/cm at lens center, the effective length L=208.3$\pm$1.44mm along the lens axis have been obtained. The harmonic contents were determined up to 20-pole from the generalized least squares fitting. The results indicate that sextupole/quadrupole is below 1.4$\pm$0.9% and all the other multipoles are below 0.5% in the region within 18mm radius at the center of lens.

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Manufacturing and testing of flat-type divertor mockup with advanced materials

  • Nanyu Mou;Xiyang Zhang;Qianqian Lin;Xianke Yang;Le Han;Lei Cao;Damao Yao
    • Nuclear Engineering and Technology
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    • v.55 no.6
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    • pp.2139-2146
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    • 2023
  • During reactor operation, the divertor must withstand unprecedented simultaneous high heat fluxes and high-energy neutron irradiation. The extremely severe service environment of the divertor imposes a huge challenge to the bonding quality of divertor joints, i.e., the joints must withstand thermal, mechanical and neutron loads, as well as cyclic mode of operation. In this paper, potassium-doped tungsten (KW) is selected as the plasma facing material (PFM), oxygen-free copper (OFC) as the interlayer, oxide dispersion strengthened copper (ODS-Cu) alloy as the heat sink material, and reduced activation ferritic/martensitic (RAFM) steel as the structural material. In this study, a vacuum brazing technology is proposed and optimized to bond Cu and ODS-Cu alloy with the silver-free brazing material CuSnTi. The most appropriate brazing parameters are a brazing temperature of 940 ℃ and a holding time of 15 min. High-quality bonding interfaces have been successfully obtained by vacuum brazing technology, and the average shear strength of the as-obtained KW/Cu and ODS-Cu alloy joints is ~268 MPa. And a fabrication route for manufacturing the flat-type divertor target based on brazing technology is set. For evaluating the reliability of the fabrication technologies under the reactor relevant condition, the high heat flux test at 20 MW/m2 for the as-manufactured flat-type KW/Cu/ODS-Cu/RAFM mockup is carried out by using the Electron-beam Material testing Scenario (EMS-60) with water cooling. This paper reports the improved vacuum brazing technology to connect Cu to ODS-Cu alloy and summarizes the production route, high heat flux (HHF) test, the pre and post non-destructive examination, and the surface results of the flat-type KW/Cu/ODS-Cu/RAFM mockup after the HHF test. The test results demonstrate that the mockup manufactured according to the fabrication route still have structural and interfacial integrity under cyclic high heat loads.

Establishment of Winterizing Conditions and Analysis of Component Composition of Winterizing By-product in Corn Oil (옥수수기름의 탈납조건 확립 및 탈납부산물의 성분조성 분석)

  • Kim, Duk-Sook;Lee, Keun-Bo
    • Journal of the Korean Society of Food Culture
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    • v.22 no.5
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    • pp.603-608
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    • 2007
  • Optimal winterizing condition of com oil was cooling temperature of bleached oil from $105^{\circ}C$ to $40^{\circ}C$. Then, filtering it after keeping $1{\sim}2^{\circ}C$ by lowering the temperature gradually with treating perlite of 0.3%(w/w) amount about bleached oil and stirring. We could measure that triglyceride(TG) that extracted from lipid components from spent perlite(SP) obtained through filtration after winterizing by SACC method is major causing materials of clouding in com oil. The result of separating TG fraction by agentation TLC was that it classified into 4 kinds -U3, SU2, S2U, S3 type and the most were U3 type. From this, it's easy to identify cause of clouding in com oil is TG fraction and most of them form wax materials that can observed. The results were they kept clear appearance at $0^{\circ}C$ generally during 39.6 to 96.5 hours, especially the result of A sample that had the lowest temperature condition while they have some difference by condition of treating temperature.

An experimental study of smoke extraction efficiency along with ventilation building location in the mad tunnel (도로터널 내 환기소 위치별 방재 효율에 관한 실험적 연구)

  • Rie, Dong-Ho;Kim, Ha-Young;Yoon, Chan-Hoon;Kim, Jin
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.12 no.3
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    • pp.215-222
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    • 2010
  • An experimental study was carried out on a reduced scale model tunnel to investigate the efficiency of disaster prevention at underground and ground ventilation equipments for the fire in road tunnels. Based on Froude modeling, the 1/50 scaled model tunnel (20 m long) was manufactured. The vertical shafts that are used in the analysis of efficiency of disaster prevention are the two models that had considered when the real tunnels are designed and the amounts of smoke exhaust are applied the miniature of the real tunnels' smoke exhaust, 560 and $280\;m^3/s$. As the result of analysis, it is the possible the emissions of the entire quantity of CO gas through the vertical shafts. In the ground ventilation equipments, the concentration of CO is discharged 2.23~2,73 ppm smaller than the underground ventilation equipments. And the temperature rise in the ground ventilation equipments is $0.53{\sim}0.94^{\circ}C$ lower than in the underground ventilation equipments because of a cooling effect of the surface of the tunnel wall. As a result of analysis of CO concentration and the temperature rise in the modeling ventilation equipment, the position of ground ventilation equipment is more effective than the underground ventilation equipment in disaster prevention measures.

Recent Research Status of Postharvest Management of Broccoli (브로콜리 수확후 관리의 최근 연구 동향)

  • Choi, Ji-Weon;Lee, Woo-Moon;Kwak, Jung-Ho;Kim, Won-Bae;Kim, Ji-Gang;Lee, Seung-Ku;Cho, Mi-Ae
    • Journal of the Korean Society of International Agriculture
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    • v.23 no.5
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    • pp.497-502
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    • 2011
  • Broccoli is considered as one of the functional foods to offer a hearty supply of the nutrient-rich vegetable in the world. Broccoli contains high level of phytochemicals, and that is selected as one of the top 10 vegetables for human health promotion. Especially, glucosinolates and flavonoids are well known as anti free oxygen radicals in vegetables and fruits. In Korea, broccoli consumption has increased to well known on the health-beneficial vegetables since 2000. However, broccoli has many problems of postharvest management since the quality of harvested heads quickly declines. Major problems are the floret yellowing, wilting, off-odor, and decay. The multiple postharvest applications improve broccoli quality and cold treatment including pre-cooling extends on the shelf-life with circumstance of optimum storage which is 0℃ temperature and a range of 95-100% relative humidity. Controlled atmosphere or modified atmosphere packaging can be used as supplemental treatments to extend postharvest life. 1-2% O2 + 5-10% CO2 is currently recommended for broccoli. Postharvest management is important for broccoli because price fluctuations depend on harvest time and quality. In this study, we tried to review physiological change of broccoli after the harvest, storage method, and various techniques to optimize quality during distribution.

Applications of the Fast Grain Boundary Model to Cosmochemistry (빠른 입계 확산 수치 모델의 우주화학에의 적용)

  • Changkun Park
    • Korean Journal of Mineralogy and Petrology
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    • v.36 no.3
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    • pp.199-212
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    • 2023
  • Diffusion is a powerful tool to understand geological processes recorded in terrestrial rocks as well as extraterrestrial materials. Since the diffusive exchange of elements or isotopes may have occurred differently in the solar nebula (high temperature and rapid cooling) and on the parent bodies (fluid-assisted thermal metamorphism at relatively low temperature), it is particularly important to model elemental or isotopic diffusion profiles within the mineral grains to better understand the evolution of the early solar system. A numerical model with the finite difference method for the fast grain boundary diffusion was established for the exchange of elements or isotopes between constituent minerals in a closed system. The fast grain boundary diffusion numerical model was applied to 1) 26Mg variation in plagioclase of an amoeboid olivine aggregate (AOA) from a CH chondrite and 2) Fe-Mg interdiffusion between chondrules, AOA, and matrix minerals in a CO chondrite. Equilibrium isotopic fractionation and equilibrium partitioning were also included in the numerical model, based on the assumption that equilibrium can be reached at the interfaces of mineral crystals. The numerical model showed that diffusion profiles observed in chondrite samples likely resulted from the diffusive exchange of elements or isotopes between the constituent minerals. This study also showed that the closure temperature is determined not only by the mineral with the slowest diffusivity in the system, but also strongly depends on the constituent mineral abundances.