• Journal of Plant Biotechnology
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• v.48 no.3
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• pp.165-172
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• 2021

The solubilization of isolated proteins into the adequate buffer containing of surfactants is primary step for proteomic analysis. Particularly, sodium dodecyl sulfate (SDS) is the most widely used surfactant, however, it is not compatible with mass spectrometry (MS). Therefore, it must be removed prior to MS analysis through rigorous washing, which eventually results in inevitable protein loss. Recently, photocleavable surfactant, 4-hexylphenylazosulfonate (Azo), was reported which can be easily degraded by UV irradiation and is compatible with MS during proteomic approach using animal tissues. In this study, we employed comparative label-free proteomic analysis for evaluating the solubilization efficacies of the Azo and SDS surfactants using rice leave proteins. This approach led to identification of 3,365 proteins of which 682 proteins were determined as significantly modulated. Further, according to the subcellular localization prediction in SDS and Azo, proteins localized in the chloroplast were the major organelle accounting for 64% of the total organelle in the SDS sample, while only 37.5% of organelle proteins solubilized in the Azo were predicted to be localized in chloroplast. Taken together, this study validates the efficient solubilization of total protein isolated from plant material for bottom-up proteomics. Azo surfactant is suitable as substitute of SDS and promising for bottom-up proteomics as it facilitates robust protein extraction, rapid washing step during enzymatic digestion, and MS analysis.

• Journal of the Society of Naval Architects of Korea
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• v.41 no.2
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• pp.79-89
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• 2004

The viscous free surface flow around KRISO container ship (KCS) is computed using the finite volume based multi block RANS code, WAVIS developed at KRISO. The free surface is captured with the Level-Set method and the realizable k-$\varepsilon$ model is employed for turbulence closure. The computations are carried out at model scale. For accurate free surface solution and its stable convergence the computations are performed with a suitable grid refinement around the free surface by applying an implicit discretization method based on a finite volume method to the Level-Set formulation. In all computational cases the numerical results agree well with experimental measurements.

• Journal of information and communication convergence engineering
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• v.17 no.1
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• pp.60-66
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• 2019

In this paper, we propose a new free-view three-dimensional (3D) computational reconstruction of integral imaging to improve the visual quality of reconstructed 3D images when low-resolution elemental images are used. In a conventional free-view reconstruction, the visual quality of the reconstructed 3D images is insufficient to provide 3D information to applications because of the shift and sum process. In addition, its processing speed is slow. To solve these problems, our proposed method uses a pixel rearrangement technique (PERT) with locally selective elemental images. In general, PERT can reconstruct 3D images with a high visual quality at a fast processing speed. However, PERT cannot provide a free-view reconstruction. Therefore, using our proposed method, free-view reconstructed 3D images with high visual qualities can be generated when low-resolution elemental images are used. To show the feasibility of our proposed method, we applied it to optical experiments.

• 한국전산유체공학회:학술대회논문집
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• 2009.11a
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• pp.49-55
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• 2009

This paper evaluates performances of a recently developed divergence-free finite element method based on Hermite interpolated stream functions. Velocity bases are derived from Hermite interpolated stream functions to form divergence-free basis functions. These velocity basis functions constitute a solenoidal function space, and the simple gradient of the Hermite functions constitute an irrotational function space. The incompressible Navier-Stokes equation is orthogonally decomposed into a solenoidal and an irrotational parts, and the decoupled Navier-Stokes equations are projected onto their corresponding spaces to form proper variational formulations. To access accuracy and convergence of the present algorithm, three test problems are selected. They are lid-driven cavity flow, flow over a backward-facing step and buoyancy-driven flow within a square enclosure. Hermite interpolation functions from cubic to quintic are chosen to run the test problems. Numerical results are shown. In all cases it has shown that the present method has performed well in accuracies and convergences. Moreover, the present method does not require an upwinding or a stabilized term.

• Journal of the Optical Society of Korea
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• v.19 no.1
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• pp.45-54
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• 2015

In this study, a novel parallel wavefront correction system architecture is proposed, and a model-based tabu search (MBTS) algorithm is introduced for this new system to compensate wavefront aberration caused by atmospheric turbulence in a free-space optical (FSO) communication system. The algorithm flowchart is presented, and a simple hypothetical design for the parallel correction system with multiple adaptive optical (AO) subsystems is given. The simulated performance of MBTS for an AO-FSO system is analyzed. The results indicate that the proposed algorithm offers better performance in wavefront aberration compensation, coupling efficiency, and convergence speed than a stochastic parallel gradient descent (SPGD) algorithm.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1996.10a
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• pp.55-61
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• 1996

In the present study, a numerical formulation procedure fer free vibration analysis of thin-walled horizontally curved multi-girder bridges is presented. The presented finite element procedure consists of curved and straight beam elements including warping degree of freedom. The homogeneous solutions of curved beam equations were used for shape functions in numerical formulation to achieve good convergence. In the straight beam element, the third order hermite polynomials were used fer shape functions. The Gupta method was used to solve the eigenvalue problem efficiently. The developed numerical procedure was applied to investigate the characteristics of free vibration of horizontally curved multi-girder bridges with varing subtended angle.

• 한국기계연구소 소보
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• v.4 no.1
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• pp.29-42
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• 1981

In this paper, it is demonstrated that, with the distribution of lowestorder concentrated (discrete) singularities of delta function nature, the solution to the linear free surface problem can be obtaianed with a remarkable degree of accuracy. The linearized bounday valve problem is formulated subject to boundary conditions for the determination of strengths of singularities; the simple sources above (not on) the free surface and the vortices on the body surface. Three sample calculations were performed; the flow about a submerged vortex of known strength, the flow past a submerged circular cylinder, and the flow around a hydrofoil section. The convergence of the numerical procedure is achieved with a relatively small number of elements, The final results are compared with those of the publi¬shed works, and are considered very satisfactory.

• Journal of Mechanical Science and Technology
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• v.20 no.11
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• pp.1881-1890
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• 2006

In this paper, a Petrov-Galerkin natural element method (PG-NEM) based upon the natural neighbor concept is presented for the free vibration and dynamic response analyses of two-dimensional linear elastic structures. A problem domain is discretized with a finite number of nodes and the trial basis functions are defined with the help of the Voronoi diagram. Meanwhile, the test basis functions are supported by Delaunay triangles for the accurate and easy numerical integration with the conventional Gauss quadrature rule. The numerical accuracy and stability of the proposed method are verified through illustrative numerical tests.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.21 no.2
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• pp.186-193
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• 2011

The NDIF method(non-dimensional dynamic influence function method) for free vibration analysis of arbitrarily shaped plates with the simply supported edge is newly developed in the paper. In order to extract the system matrix that gives the natural frequencies and natural modes of the plate of interest, the difficulty of measuring higher differential terms involved in the simply supported boundary condition is successfully overcome. Finally, the excellence of the characteristics of convergence and accuracy of the proposed method is shown through two verification examples, which indicate that natural frequencies and natural modes obtained by the proposed method are very accurate and swiftly converged even though a small number of nodes are used compared with FEM.

• Journal of Ocean Engineering and Technology
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• v.25 no.5
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• pp.9-14
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• 2011

The divergence-free finite elements introduced in this paper are derived from Hermite functions, which interpolate stream functions. Velocity bases are derived from the curl of the Hermite functions. These velocity basis functions constitute a solenoidal function space, and the gradient of the Hermite functions constitute an irrotational function space. The incompressible Navier-Stokes equation is orthogonally decomposed into its solenoidal and irrotational parts, and the decoupled Navier-Stokes equations are then projected onto their corresponding spaces to form appropriate variational formulations. The degrees of the Hermite functions we introduce in this paper are bi-cubis, quartic, and quintic. To verify the accuracy and convergence of the present method, three well-known benchmark problems are chosen. These are lid-driven cavity flow, flow over a backward facing step, and buoyancy-driven flow within a square enclosure. The numerical results show good agreement with the previously published results in all cases.