• Archives of Pharmacal Research
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• v.25 no.6
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• pp.932-939
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• 2002

The aim of the present study was to determine the characteristics of cytisine on the secretion of catecholamines (CA) in isolated perfused rat adrenal glands, and to clarify its mechanism of action. The release of CA evoked by the continuous infusion of cytisine ($1.5{\times}10^{-5} M$) was time-dependently reduced from 15 min following the initiation of cytisine infusion. Furthermore, upon the repeated injection of cytisine ($5{\times}10^{-5}$), at 30 min intervals into an adrenal vein, the secretion of CA was rapidly decreased following the second injection. Tachyphylaxis to the release of CA was observed by the repeated administration of cytisine. The cytisine-induced secretion of CA was markedly inhibited by pretreatment with chlorisondamine, nicardipine, TMB-8, and the perfusion of $Ca^{2+}$-free Krebs solution, while it was not affected by pirenzepine or diphenhydramine. Moreover, the secretion of CA evoked by ACh was time-dependently inhibited by the prior perfusion of cytisine ($5{\times}10^{-6} M$). Taken together, these experimental data suggest that cytisine causes secretion of catecholamines from the perfused rat adrenal glands in a calcium-dependent fashion through the activation of neuronal nicotinic ACh receptors located in adrenomedullary chromaffin cells. It also seems that the cytisine-evoked release of catecholamine is not relevant to the activation of cholinergic M$_1$-muscarinic or histaminergic receptors.

• Journal of Pharmaceutical Investigation
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• v.36 no.2
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• pp.115-121
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• 2006

The aim of this study was to develop new protein/peptide depot system instead of poly(DL-lactic acid-coglycolic acid) (PLGA) microspheres. Pullulan was chemically modified by the addition of acetic anhydride (pullulan acetate; PA) and then investigated as new depot system for protein/peptide delivery. PA microspheres (PAM) with lysozyme as a model protein were prepared by w/o/w double emulsion method. The microspheres had a mean size of 10-50 mm with a spherical shape. The size distributions reduced with increasing the degree of acetylation. The loading efficiency of lysozyme was also increased. Lysozyme aggregation behavior in the microsphere was monitored to estimate the change of protein stability during preparation step. The ratios of protein aggregation in PAMs are lower than that of PLGA microsphere, in particular, PA 5 showed lowest as about 16%. The result indicated that the increase of acetylation suppressed the aggregation of protein. The release profiles of lysozyme from PAMs were significantly different. High acetylation effectively improved lysozyme release kinetics by reducing initial burst release and extending continuous release over a period of time. To check the effect of preservation for structural stability of lysozyme, the activity of lysozyme released from PA 5 was also observed. The activity of lysozyme was maintained almost 100% for 25 day. Therefore, PAM may become to a useful carrier for delivery of protein/peptide drugs, if it will be supported by biocompatibility and biodegradability results.

• Macromolecular Research
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• v.17 no.12
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• pp.1010-1014
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• 2009

Monodispersed microparticles with a poly(D,L-lactide-co-glycolide) (PLGA) core and a poly(ethyl 2-cyanoacrylate) (PE2CA) shell were prepared by Shirasu porous glass (SPG) membrane emulsification to reduce the initial burst release of doxorubicin (DOX). Solution mixtures with different weight ratios of PLGA polymer and E2CA monomer were permeated under pressure through an SPG membrane with $1.9\;{\mu}m$ pore size into a continuous water phase with sodium lauryl sulfate as a surfactant. Core-shell structured microparticles were formed by the mechanism of anionic interfacial polymerization of E2CA and precipitation of both polymers. The average diameter of the resulting microparticles with various PLGA:E2CA ratios ranged from 1.42 to $2.73\;{\mu}m$. The morphology and core-shell structure of the microparticles were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The DOX release profiles revealed that the microparticles with an equivalent PLGA:E2CA weight ratio of 1:1 exhibited the optimal condition to reduce the initial burst of DOX. The initial release rate of DOX was dependent on the PLGA:E2CA ratio, and was minimized at a 1:1 ratio.

• International Journal of Concrete Structures and Materials
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• v.18 no.3E
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• pp.213-219
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• 2006

Modified singular fracture process zone(S-FPZ) model is proposed in this paper to determine a fracture criterion for continuous crack propagation in concrete. The investigated fracture properties of the proposed fracture model are strain energy release rate at a micro-crack tip and the relationship between crack closure stress(CCS) and crack opening displacement(COD) in the FPZ. The proposed model can simulate the actual fracture energy of experimental results fairly well. The results of the experimental data analysis show that specimen geometry and loading condition did not affect the CCS-COD relation. However, the strain energy release rate is a function of not only specimen geometry but also crack extension. The strain energy release rate remained constantly at the minimum value up to the crack extension of 25 mm, and then it increased linearly to the maximum value. The maximum fracture criterion occurred at the peak load for specimens of large size. The fracture criterion remained at the maximum value after the peak load. The variation of the fracture criterion is caused by micro-cracking and micro-crack localization. The fracture criterion of strain energy release rate can simply be the size effect of concrete fracture, and it can be used to quantify the micro-cracking and micro-crack localizing behavior of concrete.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.10
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• pp.5244-5259
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• 2019

With the continuous development of LBS (Location Based Service) applications, privacy protection has become an urgent problem to be solved. Differential privacy technology is based on strict mathematical theory that provides strong privacy guarantees where it supposes that the attacker has the worst-case background knowledge and that knowledge has been applied to different research directions such as data query, release, and mining. The difficulty of this research is how to ensure data availability while protecting privacy. Spatial multidimensional data are usually released by partitioning the domain into disjointed subsets, then generating a hierarchical index. The traditional data-dependent partition methods need to allocate a part of the privacy budgets for the partitioning process and split the budget among all the steps, which is inefficient. To address such issues, a novel two-step partition algorithm is proposed. First, we partition the original dataset into fixed grids, inject noise and synthesize a dataset according to the noisy count. Second, we perform IH-Tree (Improved H-Tree) partition on the synthetic dataset and use the resulting partition keys to split the original dataset. The algorithm can save the privacy budget allocated to the partitioning process and obtain a more accurate release. The algorithm has been tested on three real-world datasets and compares the accuracy with the state-of-the-art algorithms. The experimental results show that the relative errors of the range query are considerably reduced, especially on the large scale dataset.

• Macromolecular Research
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• v.16 no.4
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• pp.303-307
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• 2008

A series of biodegradable polymeric scaffolds was prepared by using a combination of natural (collagen) and synthetic (poly(caprolactone)) (PCL) polymers in various compositions. These scaffolds were soft, spongy, porous and transparent in nature and were characterized by thermogravimetric analysis (TGA) and Fourier transform infrared (FT-IR) spectroscopy. The entrapment efficiency and drug release activity of the scaffolds were analyzed using penicillin and tetracycline as antimicrobial drugs. The drug release activity of the scaffolds with various combinations of collagen and PCL were studied by measuring the optical density in a spectrophotometer at the following time intervals: 1,4, 24, 48 and 60 h. These scaffolds showed better and continuous drug release for up to 60 h. Even after such a long duration, a portion of the drug remained entrapped in the scaffolds, indicating that they can be utilized for wound healing applications.

• Journal of the Korean Physical Society
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• v.73 no.9
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• pp.1279-1282
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• 2018

This paper reports the deposition of a metal line using a multilayer stack and laser-induced forward transfer (LIFT) using a low cost continuous wave blue laser with a wavelength of 450 nm. The donor structure was composed of a light-to-heat (LTH) layer, a release layer, and a transfer layer in series. Amorphous silicon as the LTH layer absorbs photon energy and converts it to heat. A release layer was melted so that a silver transfer layer would be transferred to the receiver substrate. The transferred silver layer showed reasonable physical and electrical characteristics. A low cost fine linewidth metal layer could be achieved using this modified LIFT technique and blue laser.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.6
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• pp.599-602
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• 1997

Fluctuation of fungal zoospores on agar strips were observed in the rumen of sheep fed three different levels of dietary concentrate, timothy hay: concentrate = 3:0 (AF diet), timothy hay: concentrate = 2:1 (MC diet), timothy hay : concentrate = 1:2 (HC diet) respectively. The number of zoospores on the strip was drastically decreased after morning feed with AF diet. The number was the highest at 0 h ($1.34{\times}10^2/cm^2$), then declined to $2.0{\times}10^3/cm^2$ at 9 h after feeding. In the rumen of animals fed MC diet, the number of zoospores decreased with time after feeding, although the decrement was slower than that with AF diet. During 0-3 h after feeding, number of zoospores was $1.6{\times}10^4/cm^2$. Although the number slightly decreased at 6 and 9 h, relatively high levels were maintained. It seems that the inducers for zoospore-release were maintained at relatively high concentration throughout incubation period. The fluctuation pattern of number of germinated zoospores was different in the rumen of animals fed HC diet from those of AF and MC diets. The number of zoospores was constantly maintained at lower level ($1.0{\times}10^3/cm^2$) than the other diets. For MC diet, continuous high number of germinated zoospores may be due to the continuous release of zoospores by hemes in timothy hay and concentrate feed, and by unknown mechanisms. Unlike AF diet which promoted relatively rapid decline of zoosporogenesis, supplementation of concentrate feed to the timothy hay did not promote such rapid decline of zoosporogenesis. It was suggested that release of inducers for zoosporogenesis from concentrate feed persisted longer time than from timothy hay. HC diet promoted the lowest zoospore production, suggested the lowest fungal population size in this experiment. These results show that an appropriate amount of concentrate may support fungal growth and stimulate zoosporogenesis in the rumen.

• Journal of the Institute of Convergence Signal Processing
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• v.14 no.4
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• pp.222-230
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• 2013

Retinal bipolar cells according to the light stimulus respond to potential slowly, emit neurotransmitter release(glutamine acid) to depend on membrane potential. In this paper, the several physiological information on neurotransmitter release mechanism in the presynaptic terminal of the ON-type bipolar cells are incorporated into the formula model. The source of fast components and slow components of neurotransmitter release was arranged in parallel, this model was able to reproduce the membrane potential and intracellular $Ca^{2+}$ concentration dependence of neurotransmitter release faithfully. In addition, because the fast releasable components of neurotransmitter was represented by the membrane potential dependence of trapezoid type, whereas the slow releasable components was represented by the membrane potential dependence of a bell type, $Ca^{2+}$ concentration rise in intracellular is suppressed by $Ca^{2+}$ buffer to reduce slow releasable components, it was confirmed that the membrane potential dependence of neurotransmitter release was characteristics of a trapezoid type. And, in the light response of ON type bipolar cell, the result of the simulation of the neurotransmitter release caused by the components of transient and persistent was that the start of light response occurred the fast release of neurotransmitter, it was confirmed that the transient component and persistent component of the light response occurred the slow release. It was confirmed that the later of persistent component of the light response occurred due to the continuous release by synaptic vesicle supplemented from the storage pool.

• Journal of the Korean Society of Costume
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• v.16
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• pp.173-185
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• 1991

We measured change of peripheral skin temperature and mean skin temperature when the upper arm and thigh pressured in order to know the effect of skin pressure applied by clothing on blood circulation. After release from pressure, we observed also recovery condition. At the same time, we examined relation between pressure and a feeling of tightness. Three physiques of healthy females, namely slender, standard and plump, served as subjects. We used intermittent method with skin pressure applied by experimental fabric at l-minute intervals. Besides we made a comparative study with results according to different restraint method (continuous method and intermittent method). As a result of this experiment, we obtained following findings. 1. The significant difference was marked at the pressure, measuring time, physique and measuring region with change of skin temperature under upper arm and thigh restraint by intermittent method. The peripheral skin temperature decreased with the lapse of restraint time. A remarkable tendency observed according to the. increase of restraint pressure. Recovery condition after release from pressure not yet recovered to original state, for all after a lapse of 10 min. The mean skin temperature decreased with the lapse of restraint time in case of upper arm restraint, it was not an obvious tendency except 60mmHg under thigh restraint. 2. Main factor affecting the evaluation of a feeling of tightness was restraint pressure, when the upper arm and thigh restraint by intermittent method. The respondence rate of 'very tight' grew larger according to the increase of restraint pressure. The value of pressure sensation declined after restraint ten times as compared with one time, but there was difference according to restraint pressure. 3. We reexamined change of skin temperature and feeling of tightness by different restraint method(continuous method and intermittent method). The results were as follows. 1) The skin temperature decreased more greatly during skin pressure by continuous method than intermittent method, especially in the peripheral. Without different restraint method, the skin temperature of slender plysique decreased more greatly than that of plump physique. 2) The value of pressure sensation by intermittent method was highly on both sites of upper arm and thigh.