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Glycosyl glycerides from hydroponic Panax ginseng inhibited NO production in lipopolysaccharide-stimulated RAW264.7 cells

  • Cha, Byeong-Ju;Park, Ji-Hae;Shrestha, Sabina;Baek, Nam-In;Lee, Sang Min;Lee, Tae Hoon;Kim, Jiyoung;Kim, Geum-Soog;Kim, Seung-Yu;Lee, Dae-Young
    • Journal of Ginseng Research
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    • v.39 no.2
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    • pp.162-168
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    • 2015
  • Background: Although the aerial parts of hydroponic Panax ginseng are reported to contain higher contents of total ginsenosides than those of roots, the isolation and identification of active metabolites from the aerial parts of hydroponic P. ginseng have not been carried out so far. Methods: The aerial parts of hydroponic P. ginseng were applied on repeated silica gel and octadecylsilane columns to yield four glycosyl glycerides (Compounds 1-4), which were identified based on nuclear magnetic resonance, infrared, fast atom bombardment mass spectrometry, and gas chromatography/mass spectrometry data. Compounds 1-4 were evaluated for inhibition activity on NO production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Results and conclusion: The glycosyl glycerides were identified to be (2S)-1-O-7(Z),10(Z),13(Z)-hexadecatrienoyl-3-O-${\beta}$-$\small{D}$-galactopyranosyl-sn-glycerol (1), (2S)-1-O-linolenoyl-3-O-${\beta}$-$\small{D}$-galactopyranosyl-sn-glycerol (2), (2S)-1-O-linolenoyl-2-O-linolenoyl-3-O-${\beta}$-$\small{D}$-galactopyranosyl-sn-glycerol (3), and 2(S)-1-O-linoleoyl-2-O-linoleoyl-3-O-${\beta}$-$\small{D}$-galactopyranosyl-sn-glycerol (4). Compounds 1 and 2 showed moderate inhibition activity on NO production in LPS-stimulated RAW264.7 cells [half maximal inhibitory concentration ($IC_{50}$): $63.8{\pm}6.4{\mu}M$ and $59.4{\pm}6.8{\mu}M$, respectively] without cytotoxicity at concentrations < $100{\mu}M$, whereas Compounds 3 and 4 showed good inhibition effect ($IC_{50}$: $7.7{\pm}0.6{\mu}M$ and $8.0{\pm}0.9{\mu}M$, respectively) without cytotoxicity at concentrations < $20{\mu}M$. All isolated compounds showed reduced messenger RNA (mRNA) expression of interleukin-$1{\beta}$ (IL-$1{\beta}$), IL-6, and tumor necrosis factor-${\alpha}$ in LPS-induced macrophage cells with strong inhibition of mRNA activity observed for Compounds 3 and 4.

Study on the Fish Community in the Seagrass Belt around Cheju Island II. Growth, reproduction and food habit of Tubesnout, Aulichthys japonicus Brevoort (제주도 연안 해초지대의 어류군집에 관한 연구 II. 실비늘치 (Aulichthys japonicus Brevoort)의 성장, 산란 및 식성)

  • Go, You-Bong;Cho, Sung-Hwan;Go, Gyung-Min
    • Korean Journal of Ichthyology
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    • v.9 no.1
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    • pp.61-70
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    • 1997
  • The spawning season, growth and feeding habit of tubesnout, Aulichthys japonicus were examined from the samples collected by a small beam trawl in the Zostera marina belt at the coastal water off Hamduck, Cheju Island, from May 1993 to May 1994. Tubesnout was the dominant species of overall 58 species collected in the study area throughout the year. The larvae and juvenile of tubesnout in the frequency distributions of body length began to appear in the Z. marina belt in March, and adults disappeared after May in the following year. The gonad weight index (GWI) and the condition factor (K) were high from November 1993 to January 1994 for males, while females were high from February to May 1994. Although tubesnout is an oviparous fish, males have the urogenital papilla. These results suggest that they were fertilized between November and January, and then males seemed to be died or move into other places. Females were examined to spawn from February to May. The stomach contents of tubesnout were dominated by copepoda smaller than ca. 1mm, Paracalanus, Oithona, Acartia, Oncaea and Harpacticoidae, accounting for 97% of total food items. The feeding habit of tubesnout did not vary by size. The fishes fed on Calanus, Euchaeta and decapoda larvae larger than ca. 3mm in March and April during the spawning season.

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The Study of Evaluation of University Students' Health Behaviors (대학생의 건강행위 평가 연구)

  • Lee, Grace Changkeum;Park, Yeon-Suk
    • Journal of Digital Convergence
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    • v.15 no.12
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    • pp.517-529
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    • 2017
  • The purpose of this study is to develop and evaluate the scale for university students' health behaviors. This research is based on Roger's(1975) Protection Motivation Theory and Weinstein(1988)'s Precaution Adoption Process Model as theoretical frameworks. The contents of the scale were first abstracted from personal depth interviews of qualitative study on university students' health behavior experiences and review of its related literature. The initial items consisted of 76 behavior questions and 34 perception questions. After conducting the preliminary test, 33 behavior questions and 24 perception questions were selected for the final test by revisions on the basis of the result of the content validity test and the item understanding test. The questionnaires for the final test were distributed to 400 students enrolled in three universities. The questionnaires collected from 373 participants were analyzed by the SPSS program. From the examination of construct validity, item correlation, and factor relation turned out to be relevant. Five factors were determined from 27 items with a cumulative explained variance of 54.498 %. After examining reliability and validity of the collected dat, 27 items were finalized as the scale for university students' health behaviors.

Analysis on the development necessity of a safety health knowledge test for young children (유아 안전보건지식 측정도구 개발 필요성에 대한 분석)

  • Kwak, Eunbog;Choi, Gyu yil
    • Journal of Digital Convergence
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    • v.18 no.8
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    • pp.511-518
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    • 2020
  • The purpose of this study is to emphasize the importance of the safety and health ability to safely protect body in a risky situation in infancy, but due to the lack of a valid test tool for measuring infant safety knowledge, measure the safety and health knowledge of infants. In order to achieve the purpose of the study, opinions of education experts and early childhood education experts were collected, and suitable factors for measuring safety and health knowledge of children aged 3-5 years were extracted. The measurement questions developed based on this were surveyed on 186 children aged 3-5 years old to verify the relevance of the question composition and the reliability of the contents. In the distribution of the item response, values of 0.8 or less were deleted, and items with a correlation of r<.40 or less were also deleted, and the reliability test showed more than .70. After exploratory factor analysis, it was developed into 23 questions in 3 areas. The total score of safety and health knowledge for the final question and the degree of correlation were above .80. The tool for measuring child safety and health knowledge developed in this study was confirmed to be appropriate for item discrimination, item reliability, and content validity, and thus will be used as basic data for measuring child safety and health knowledge. After the early childhood safety and health education program is implemented, Further research should verify the effectiveness of the early childhood safety and health program.

AN EXPERIMENTAL STUDY ON THE BONE INDUCTION CAPACITY OF THE PORCINE BONE MATRIX-DERIVED BONE MORPHOGENETIC PROTEIN (돼지의 골기질유도 골형성단백질의 골유도능에 관한 실험적 연구)

  • Park, Young-Wook;Lee, Jong-Ho;Kim, Soo-Kyeong
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.19 no.3
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    • pp.265-286
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    • 1997
  • Bone morphogenetic proteins(BMPs) are a group of transforming growth factor beta(TGF-${\beta}$)-related factors and multifunctional proteins, especially the only known biologic factors capable of inducing endochondral bone formation at an extraskeletal site. This study was performed to investigate the effect of the partially purified porcine BMP(pBMP) at an ectopic site. PBMP was partially purified from porcine bone matrix and its activity was monitored by an in vivo bioassay. The purification method utilized extraction of the bone-inducing activity with 4M guanidine, followed by chromatography on heparin-Sepharose. Active fractions were assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. And the fractions were reconstituted with inactive insoluble collagenous bone matrix from rats, acid soluble type I collagen from rat tail and chondroitin-6-sulfate sodium salt and implanted into the pectroralis muscle pouches of Sprague-Dawley rats. And the carrier complex was implanted on the opposite side as control. The rats were sacrificed at the day of 1st, 3rd, 5th, 7th, 11th, 14th and 21st after implantation and examined histologically, radiologically and biochemically. And alkaline phosphatase activity and calcium content were used as indices of bone formation. The results were as follows ; 1. Active fractions were localized in a zone between 31 and 40 KDa on SDS-PAGE. 2. The implanted 3.0mg of the partially purified pBMP induced cartilage and bone in the muscle tissue of rats through an endochondral ossification process. 3. Inactive insoluble bone matrix, type I collagen and chondroitin-6-sulfate have functioned as carriers for pBMP, but revealed some foreign body reactions. 4. Soft X-ray didn't reveal significant change between the experimental and the control group. 5. The alkaline phosphatase activities in the experimental group of 5th, 7th, 11th, 14th and 21st were increased significantly compared with control (p<0.01) with the peak in the group of 11th day. 6. With time, the calcium content of the experimental group increased. And the calcium contents in the experimental group of 11th, 14th and 21st were increased significantly compared with control (p<0.01).

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Creep Behavior of Pultruded Ribbed GFRP Rebar and GFRP Reinforced Concrete Member (인발성형된 이형 GFRP 보강근과 GFRP 보강 콘크리트 부재의 크리프 거동)

  • You, Young-Jun;Park, Young-Hwan;Kim, Hyung-Yeol;Choi, Jin-Won;Kim, Jang-Ho Jay
    • Journal of the Korea Concrete Institute
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    • v.25 no.2
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    • pp.187-194
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    • 2013
  • Fiber reinforced polymer (FRP) has been gathering interest from designers and engineers for its possible usage as a replacement reinforcement of a steel reinforcing bar due to its advantageous characteristics such as high tensile strength, non-corrosive material, etc. Since it is manufactured with various contents ratios, fiber types, and shapes without any general specification, test results for concrete members reinforced with these FRP reinforcing bars could not be systematically used. Moreover, since investigations for FRP reinforced members have mainly focused on short-term behavior, the purpose of this study is to evaluate long-term behaviors of glass FRP (GFRP) reinforcing bar and concrete beams reinforced with GFRP. In this paper, test results of tensile and bond performance of GFRP reinforcing bar and creep behavior are presented. In the creep tests, results showed that 100 years of service time can be secured when sustained load level is below 55% of tensile strength of GFRP reinforcing bar. A modification factor of 0.73 used to calculate long-term deflection of GFRP reinforced beams was acquired from the creep tests for GFRP reinforced concrete beams. It is expected that these test results would give more useful information for design of FRP reinforced members.

Changes in Growth and Nutrient Contents of Populus ablba var. pyramidalis treated with Fertilizers and Absorbents in an Arid Region of China (중국 건조지역에서 시비와 보수제 처리에 의한 신강포플러의 생장 및 묘목의 양분 함량 변화)

  • Kyung, Ji Hyun;Son, Yohwan;Noh, Nam Jin;Yi, Myong Jong;Lee, Chun Yong;Youn, Ho Joong
    • Journal of Korean Society of Forest Science
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    • v.95 no.6
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    • pp.751-758
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    • 2006
  • To investigate soil management practices for increasing tree growth in arid regions of China, Xinjiang poplar (Populus alba var. pyramidalis) seedlings were planted in a nursery and windbreak and were treated with fertilizers (nitrogen and phosphorus) and absorbents (artificial; K-SAM and natural: Yuho (Artemisia ordosica)) at Dengkou in Inner Mongolia. Seedling height, diameter at breast height (DBH), biomass, and nitrogen (N) and phosphorus (P) concentrations were measured 1.5 years after the treatments. Soil texture was sandy loam for nursery and clay loam for windbreak, soil pH was 8.78 for nursery and 8.51 for windbreak, and total soil N concentration was 0.06% for nursey and 0.08% for windbreak. [n general, seedling height and DBH increased in the N fertilizer treatment compared to control or other treatments both in nursery and windbreak. Also seedling growth increased after the treatments of artificial and natural absorbents in windbreak. Total above- and belowground biomass increased after the N + P treatment. Foliage N and P concentrations following N and/or P treatments were same in the nursey, however, they significantly increased in the windbreak indicating N deficiency. It appeared that N was a limiting factor for early growth of Xinjiang poplar seedlings and artificial and natural absorbents (K-SAM and Yuho) would be useful to increase seedling growth in the arid region.

A Collision detection from division space for performance improvement of MMORPG game engine (MMORPG 게임엔진의 성능개선을 위한 분할공간에서의 충돌검출)

  • Lee, Sung-Ug
    • The KIPS Transactions:PartB
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    • v.10B no.5
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    • pp.567-574
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    • 2003
  • Application field of third dimension graphic is becoming diversification by the fast development of hardware recently. Various theory of details technology necessary to design game such as 3D MMORPG (Massive Multi-play Online Role Flaying Game) that do with third dimension. Cyber city should be absorbed. It is the detection speed that this treatise is necessary in game engine design. 3D MMORPG game engine has much factor that influence to speed as well as rendering processing because it express huge third dimension city´s grate many building and individual fast effectively by real time. This treatise nay get concept about the collision in 3D MMORPG and detection speed elevation of game engine through improved detection method. Space division is need to process fast dynamically wide outside that is 3D MMORPG´s main detection target. 3D is constructed with tree construct individual that need collision using processing geometry dataset that is given through new graph. We may search individual that need in collision detection and improve the collision detection speed as using hierarchical bounding box that use it with detection volume. Octree that will use by division octree is used mainly to express rightly static object but this paper use limited OSP by limited space division structure to use this in dynamic environment. Limited OSP space use limited space with method that divide square to classify typically complicated 3D space´s object. Through this detection, this paper propose follow contents, first, this detection may judge collision detection at early time without doing all polygon´s collision examination. Second, this paper may improve detection efficiency of game engine through and then reduce detection time because detection time of bounding box´s collision detection.

Entrepreneurship Education in Internet Business: Current Status & Future Direction (인터넷 창업교육 현황과 발전방안에 관한 연구)

  • Jeong, Jin-Su;Choi, Jong-In
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.3 no.1
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    • pp.29-55
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    • 2008
  • The objective of Starts-up education rears leading company that minimize a risk & connect them with successful idea. Internet Starts-up education is serviced in public & civil parts, it is insufficient to reach successful Starts-up support. This study is targeting on review the present condition of the Internet Starts-up education, research which it sees Internet Starts-up education actual condition investigation through investigation student of education satisfactorily and education contents and the plan to grope an Starts-up education progress coat problem and developmental plan it did. The current events point which has become research result escape first, age of the students of education, followed in educational background and, education motive and education experience presence education satisfactory boat song it appeared in these demand different, base grudge the form which is various it fixed and the education was demanded. Second, currently the department of pedagogy joining in establishment leader against the technical element which is advanced swip, the education reinforcement against an enterprise accomplishment ability back human factor, an enterprise propulsion environment and an enterprise item back enterprise environment element was demanded. Time after support and consulting, completion lifestyle community construction, completion lifestyle for item connection support and the trade name net after third, establishing an enterprise we the king, after toe ring back educating which carries on shoulder the importance of the civil official was embossed. The study which it sees led and like this investigation analysis the education progress for the successful support of the Internet Starts-up and time after connection setup construction it led and the plan which it will put out advancement of Starts-up education and it groped.

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Genome size of 15 Lamiaceae taxa in Korea (한국산 꿀풀과 15 분류군에 대한 유전체양 조사)

  • Lee, Yoonkyung;Kim, Sangtae
    • Korean Journal of Plant Taxonomy
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    • v.47 no.2
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    • pp.161-169
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    • 2017
  • The genome size is one of the basic characters of an organism, and it is widely applied in various fields of biology, such as systematics, breeding biology, population biology, and evolutionary biology. This factor was recently highlighted in genome studies because choosing a representative of a plant group having the smallest genome size is important for the efficiency of a genome project. For the estimation of the genome size, flow cytometry has recently been highlighted because it is a convenient, fast, and reliable method. In this study, we report the genome sizes of 15 taxa of Lamiaceae from nine genera distributed in Korea using flow cytometry. Data pertaining to the genome size for all of our species have not been reported thus far, and the data from Agastache, Clinopodium, Elsholtzia, and Isodon are the first reported for each genus. The genome sizes of 15 genera and 39 species were reported to the Plant DNA C-values Database (http://data.kew.org/cvalues/). Scutellaria indica L. has a genome size of 0.37 pg (1C). This is the fourth smallest value among the 98 Lamiaceae taxa in the Angiosperm DNA C-value Database, indicating that this taxon can be used as a reference species in the genome studies in Lamiaceae as a native Korean species. The largest genome size observed in this study is in Phlomis umbrosa Turcz. (1C=2.60 pg), representing the possible polyploidy origin of this species in the family.