• Journal of Microbiology and Biotechnology
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• 제18권8호
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• pp.1353-1356
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• 2008

RNase E (Rne) plays a major role in the decay and processing of numerous RNAs in E. coli, and protein inhibitors of RNase E, RraA and RraB, have recently been discovered. Here, we report that coexpression of RraA or RraB reduces the ribonucleolytic activity in rne-deleted E. coli cells overproducing RNase ES, a Streptomyces coelicolor functional ortholog of RNase E, and consequently rescues these cells from growth arrest. These findings suggest that the regulators of ribonuclease activity have a conserved intrinsic property that effectively acts on an RNase E-like enzyme found in a distantly related bacterial species.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• 제12권6호
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• pp.616-622
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• 2000

This paper concerns the application of smoke and fire spread to road tunnel fire problems. When a road tunnel is on fire. a fire protection system of road tunnel have to offer an adequate escape space to human. Therefore, this study carried out a simulation for predicting a spreading path of smoke and fire. The evolution of the flow field is simulated with the low Reynolds number k-$\varepsilon$ turbulent model and SIMPLE algorithm based on the finite volume method.

• Proceedings of the Korean Institute of Interior Design Conference
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• 한국실내디자인학회 1999년도 춘계학술발표대회 논문집
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• pp.118-121
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• 1999

Traditional housing must be conserved simultaneously with not only the building itself, but the circumstances of the surroundings. Hence, traditional housing should be developed as a product of a culture environment. However, most cultural properties have recently been treated as only a piece of property by irresponsible maintenance practices and damage placed on private traditional housing. Apparently, it has not always been a result of dweller's negligence. Thus, various policies must be needed to effectively preserve traditional housing. To do so, this study will indicate a new direction for the circumstance of preservation through cultural employment of traditional high-class housing as well as reduce maintenance and damage for dweller's convenience. Furthermore, this study will propose a renovation for popularization of Korean cultural assets through the gradual implementation of both economic value and public worth.

• Journal of Life Science
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• 제9권2호
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• pp.44-48
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• 1999

The ionization of tyrosine residue is known to be involved in the stabilization of transition-state in catalysis of astacin based upon the astacin-transition state analog structure. Two tyrosine residues, Tyr-216 and Tyr-219, are conserved in all MMPs related with astacin family, We replaced Tyr-216 and Tyr-219 into phenylalanine, respectively and the zinc binding properties, kinetic parameters, and pH dependence of each mutant are determined in order to examine the role of tyrosine residue in matrilysin catalysis. Both mutants contain two zinc atoms per mol of enzyme, indicating that either tyrosime does not affect the zinc binding property of the enzyme. Y216F and Y219F mutants are highly active and the kcat/Km values are only decreased 1.1-1.5-fold compared to the wild-type enzyme. The decrease in the activity of the mutants is essentially due to the increase in Km value. The pH dependencies of the kcat/Km values for both mutants are similar to the corresponding dependencies obtained with the wild type enzyme. The pKa values at the alkaline side of both mutants are not changed. These kinetic and pH dependence results indicate that the ionization of active site tyrosine residue of matrilysin is not reflected in the kinetics of peptide hydrolysin as catalyzed by astacin.

• Journal of the Korean Home Economics Association
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• 제11권2호
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• pp.166-188
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• 1973

This thesis chiefly to investigate and study about the historic transition of the dress and ornament which is based on the costume of the folk dance. The folk dance is devide into the styled of the Court and common people I. Investigated and studied on the description of Hak-mu (the crane), Ryun-wha-dae (the lotus blossom) and chu-yong dance (a mask drama) which are the typical models among the folk dance on the view of the scale and history of playing. This research materials are made on abstract of Ak-Hak-Gyae-Bum (the pattern of the music which was compiled by Sung-hyun on the King of Sung-Jong of Yi-dynasdty) and selected from the conservation material and playing in the National Classical Music Bureau nowadays. I am sure the understanding the style of common people is to investigate and research the folk dance which is specified and conserved by cultural property control Bureau. Generally Bong-San mask dance is the typical mask drama on the view of the scale and transmitted, which occupies the wide range of the folk dance. So I selected it as the research material. I am interested in the historic origin and the structure of the dress and ormant conserved till the nowadays and so can catch the hidden conception about the colour and concern of religion in the Korean peculiar living consciousness. From this point, the study of the classical folk costume is activated in this part and I hope this poor article will become a small assistance for another study.

• Journal of the Korean Society of Manufacturing Process Engineers
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• 제21권9호
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• pp.85-91
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• 2022

The demand for electric vehicles has increased because of environmental regulations. The lithium-ion battery, the most widely used type of battery in electric vehicles, is composed of a cathode, an anode, and an electrolyte. It is manufactured according to the pole plate, assembly, and formation processes. To improve battery performance and increase manufacturing efficiency, the manufacturing process must be optimized. To do so, simulation can be used to reduce wasted resources and time, and a finite-element method can be utilized. For high simulation quality, it is essential to reflect the material properties of the electrode by considering the pores. However, the material properties of electrodes are difficult to derive through measurement. In this study, the representative volume element method, which is a homogenization method, was applied to estimate the representative material properties of the electrode considering the pores. The representative volume element method assumes that the strain energy before and after the conversion into a representative volume is conserved. The method can be converted into one representative property, even when nonhomogeneous materials are mixed in a unit volume. In this study, the material properties of the electrode considering the pores were derived. The results should be helpful in optimizing the electrode manufacturing process and related element technologies.

• Interdisciplinary Bio Central
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• 제4권4호
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• pp.11.1-11.8
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• 2012

Genes that are indispensable for survival are referred to as essential gene. Due to the momentous significance of these genes for cellular activity they can be selected potentially as drug targets. Here in this study, an essential gene for Streptococcus suis was predicted using coherent statistical analysis and powerful genome comparison computational method. At first the whole genome protein scatter plot was generated and subsequently, on the basis of statistical significance, a reference genome was chosen. The parameters set forth for selecting the reference genome was that the genome of the query (Streptococcus suis) and subject must fall in the same genus and yet they must vary to a good degree. Streptococcus pneumoniae was found to be suitable as the reference genome. A whole genome comparison was performed for the reference (Streptococcus pneumoniae) and the query genome (Streptococcus suis) and 14 conserved proteins from them were subjected to a screen for potential essential gene property. Among those 14 only one essential gene was found to be with impressive similarity score between reference and query. The essential gene encodes for a type of 'Clp protease'. Clp proteases play major roles in degrading misfolded proteins. Results found here should help formulating a drug against Strptococcus suis which is responsible for mild to severe clinical conditions in human. However, like many other computational studies, the study has to be validated furthermore through in vitro assays for concrete proof.

• Korean Institute of Interior Design Journal
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• 제29호
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• pp.58-66
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• 2001

Traditional housing must be conserved simultaneous1y with not only the building itself, but the circumstances of the surroundings. Hence, traditional housing should be developed as a product of a culture environment. However, most cultural properties have recently been treated as only a piece of property by irresponsible maintenance practices and damage placed on private traditional housing. Apparently, it has not always been a result of dweller's negligence. Thus, various policies must be needed to effectively preserve traditional housing. To do so, this study will indicate a new direction for the circumstance of preservation through cultural employment of traditional high-class housing as well as reduce maintenance and damage for dweller's convenience. Furthermore, this study will propose a renovation for popularization of Korean cultural assets through the gradual implementation of both economic vague and public worth.

• Progress in Superconductivity and Cryogenics
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• 제8권3호
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• pp.27-31
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• 2006

We propose a replication method to solve the problem of large percent failure in texturing processes of coated conductors. This problem is due to the individual texturing process for each coated conductor that is very long tape having no single defect spot. We tested two different methods of replication process that are based on a well-textured Ni template. First method has structure of Ni/BaO/STO/$CeO_2$/SmBCO/Ag. So BaO layer is dissolved in water that we can separate STO/$CeO_2$/SmBCO/Ag layers. In this processing, the property of STO is conserved but the superconductivity of SmBCO is deteriorated. So we can consider another method. Second method has structure of template(STO)/BaO/STO/Pt/STO. After dissolving BaO then we deposited YBCO. The result of these experiments showed the feasibility of replication.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• 제27권4호
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• pp.207-231
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• 2023

This paper aims to improve the alternative formulation of the fifth- and sixth-order accurate weighted essentially non-oscillatory (AWENO) finite difference schemes. The first is to derive the AWENO scheme with sixth-order accuracy in the smooth region of the solution. Second, a new weighted polynomial functions combining the perturbed forms with conserved variable to the AWENO is constructed; the new form of tunable functions are invented to maintain non-oscillatory property. Detailed numerical experiments are presented to illustrate the behavior of the new perturbational AWENO schemes. The performance of the present scheme is evaluated in terms of accuracy and resolution of discontinuities using a variety of one and two-dimensional test cases. We show that the resulted perturbational AWENO schemes can achieve fifth- and sixth-order accuracy in smooth regions while reducing numerical dissipation significantly near singularities.