• Title/Summary/Keyword: Conjugate addition

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A BAYESIAN APPROACH TO THE IMPERFECT INSPECTION MODEL

  • Park, Choon-Il
    • Journal of applied mathematics & informatics
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    • v.6 no.2
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    • pp.589-598
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    • 1999
  • Classification errors are included in sampling -with -re-placement model where items are sampled from a Bernoulli process. Bayesian imperfect inspection model is considered. In addition con-jugate prior and predctive densities for imperfect inspection model are obtained.

CONJUGATE ACTION IN A LEFT ARTINIAN RING

  • Han, Jun cheol
    • Bulletin of the Korean Mathematical Society
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    • v.32 no.1
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    • pp.35-43
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    • 1995
  • IF R is a left Artinian ring with identity, G is the group of units of R and X is the set of nonzero, nonunits of R, then G acts naturally on X by conjugation. It is shown that if the conjugate action on X by G is trivial, that is, gx = xg for all $g \in G$ and all $x \in X$, then R is a commutative ring. It is also shown that if the conjegate action on X by G is transitive, then R is a local ring and $J^2 = (0)$ where J is the Jacobson radical of R. In addition, if G is a simple group, then R is isomorphic to $Z_2 [x]/(x^2 + 1) or Z_4$.

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Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Clostridium botulinum Type F Toxin (Clostridium botulinum Type F Toxin의 면역학적 효소방법에 의한 검출에 관한 연구)

  • Lee, Jeong-Kug;K. H. Yang
    • Microbiology and Biotechnology Letters
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    • v.10 no.3
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    • pp.205-209
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    • 1982
  • The enzyme-linked immunosorbent assay using the so-called "double-sandwich"technique was applied to determine Clostridium botulinum type F toxin. Polystyrene tubes were coated with horse anti-type F toxin serum and then toxin sample was added. The tubes were subsequently treated with rabbit anti-type F toxin IgG and sheep anti-rabbit serum IgG-horseradish peroxidase conjugate. By this technique, about 10 mouse intraperitoneal 50% lethal doses (ip LD/50/) of type F toxin could be detected. Low back-ground reading was achieved with the use of phosphate-buffered saline containing 0.05% Tween 20 and 1% bovine serum albumin as diluents of rabbit IgG and conjugate. Addition of EDTA in the diluents of toxin increased ELISA extinction value significantly. No cross-reaction was observed with botulinum type A and B toxin, but type E toxin gave sleight cross-reaction.

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Multi-tissue observation of the long non-coding RNA effects on sexually biased gene expression in cattle

  • Yoon, Joon;Kim, Heebal
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.7
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    • pp.1044-1051
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    • 2019
  • Objective: Recent studies have implied that gene expression has high tissue-specificity, and therefore it is essential to investigate gene expression in a variety of tissues when performing the transcriptomic analysis. In addition, the gradual increase of long non-coding RNA (lncRNA) annotation database has increased the importance and proportion of mapped reads accordingly. Methods: We employed simple statistical models to detect the sexually biased/dimorphic genes and their conjugate lncRNAs in 40 RNA-seq samples across two factors: sex and tissue. We employed two quantification pipeline: mRNA annotation only and mRNA+lncRNA annotation. Results: As a result, the tissue-specific sexually dimorphic genes are affected by the addition of lncRNA annotation at a non-negligible level. In addition, many lncRNAs are expressed in a more tissue-specific fashion and with greater variation between tissues compared to protein-coding genes. Due to the genic region lncRNAs, the differentially expressed gene list changes, which results in certain sexually biased genes to become ambiguous across the tissues. Conclusion: In a past study, it has been reported that tissue-specific patterns can be seen throughout the differentially expressed genes between sexes in cattle. Using the same dataset, this study used a more recent reference, and the addition of conjugate lncRNA information, which revealed alterations of differentially expressed gene lists that result in an apparent distinction in the downstream analysis and interpretation. We firmly believe such misquantification of genic lncRNAs can be vital in both future and past studies.

Synergistic Antibacterial Effect and Antibacterial Action Mode of Chitosan-Ferulic Acid Conjugate against Methicillin-Resistant Staphylococcus aureus

  • Eom, Sung-Hwan;Kang, Shin-Kook;Lee, Dae-Sung;Myeong, Jeong-In;Lee, Jinhwan;Kim, Hyun-Woo;Kim, Kyoung-Ho;Je, Jae-Young;Jung, Won-Kyo;Kim, Young-Mog
    • Journal of Microbiology and Biotechnology
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    • v.26 no.4
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    • pp.784-789
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    • 2016
  • We evaluated the synergistic antibacterial effect in combination with the chitosan-ferulic acid conjugate (CFA) and β-lactam antibiotics, such as ampicillin, penicillin, and oxacillin, against methicillin-resistant Staphylococcus aureus (MRSA) using fractional inhibitory concentration (FIC) indices. CFA clearly reversed the antibacterial activity of ampicillin, penicillin, and oxacillin against MRSA in the combination mode. Among these antibiotics, the combination of oxacillin-CFA resulted in a ΣFICmin range of 0.250 and ΣFICmax of 0.563, suggesting that the oxacillin-CFA combination resulted in an antibacterial synergy effect against MRSA. In addition, we determined that CFA inhibited the mRNA expression of gene mecA and the production of PBP2a, which is a key determinant for β-lactam antibiotic resistance, in a dose-dependent manner. Thus, the results obtained in this study supported the idea on the antibacterial action mechanism that oxacillin will restore the antibacterial activity against MRSA through the suppression of PBP2a production by CFA.

Application of the Preconditioned Conjugate Gradient Method to the Generalized Finite Element Method with Global-Local Enrichment Functions (전처리된 켤레구배법의 전체-국부 확장함수를 지닌 일반유한요소해석에의 응용)

  • Choi, Won-Jeong;Kim, Min-Sook;Kim, Dae-Jin;Lee, Young-Hak;Kim, Hee-Cheul
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.24 no.4
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    • pp.405-412
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    • 2011
  • This paper introduces the generalized finite element method with global-local enrichment functions using the preconditioned conjugate gradient method. The proposed methodology is able to generate enrichment functions for problems where limited a-priori knowledge on the solution is available and to utilize a preconditioner and initial guess of good quality with only small addition of computational cost. Thus, it is very effective to analyze problems where a complex behavior is locally exhibited. Several numerical experiments are performed to confirm its effectiveness and show that it is computationally more efficient than the analysis utilizing direct solvers such as Gauss elimination method.

Total Synthesis of (±)-Aspidospermidine Starting from 3-Ethyl-5-bromo-2-pyrone

  • Cho, Hyun-Kyu;Tam, Nguyen Thanh;Cho, Cheon-Gyu
    • Bulletin of the Korean Chemical Society
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    • v.31 no.11
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    • pp.3382-3384
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    • 2010
  • A new synthetic route to ($\pm$)-aspidospermidine was devised, starting from the cycloadduct of 3-ethyl-5-bromo-2-pyrone with vinyl sulfide through a tandem conjugate addition-alkylation sequence. The requisite 3-ethyl-5-bromo-2-pyrone was prepared via the C3-selective Pd-catalyzed coupling reaction with $Et_3Al$-dimethylaminoethanol complex.

The Mixed Finite Element Analysis for Saturated Porous Media using FETI Method

  • Lee, Kyung-Jae;Tak, Moon-Ho;Park, Tae-Hyo
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.23 no.6
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    • pp.693-702
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    • 2010
  • In this paper, FETI(Finite Element Tearing and Interconnecting) method is introduced in order to improve numerical efficiency of Staggered method. The porous media theory, the Staggered method and the FETI method are briefly introduced in this paper. In addition, we account for the MPI(Message Passing Interface) library for parallel analysis, and the proposed combined Staggered method with FETI method. Finally Lagrange multipliers and CG(Conjugate Gradient) algorithm to solve decomposed domain are proposed, and then the proposed method is verified to be numerically efficient by MPI library.