• Analytical Science and Technology
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• v.8 no.4
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• pp.887-894
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• 1995

Toward the development of universal, sensitive, and convenient method of DNA (or RNA) detection, two kinds of electrochemically active DNA ligands. acridine - viologen and oligonucleotide - ferrocene conjugate, were prepared. Thermodynamic and electrochemical study revealed that these probes bound strongly to DNA, and showed a typical cyclic voltammograms, indicating a potential for use as a reversible electrochemical labelling agent for DNA. Especially, using the electrochemically active oligonucleotide, we have been able to demonstrate the detection of DNA at femtomole levels by HPLC equipped with ordinary electrochemical detector (ECD). These results lead to the conclusion that the redox-active probes are very useful for the microanalysis of nucleic acid due to the stabilily of the complexes, high detection sensitivity, and wide applicability to the target structures (single- and double strands) and sequences.

• Journal of ILASS-Korea
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• v.22 no.4
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• pp.175-184
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• 2017

The numerical investigation on the effects of water-mist characteristics has been carried out for the fire suppression mechanism. The FDS are used to simulate the interaction of fire plume and water mists, and program describes the fire-driven flows using LES turbulence model, the mixture fraction combustion model, the finite volume method of radiation transport for a non-scattering gray gas, and conjugate heat transfer between wall and gas flow. The numerical model is consisted of a rectangular enclosure of $L{\times}W{\times}H=1.5{\times}1.5{\times}2.0m^3$ and a water mist nozzle that be installed 1.8 m from fire pool. In the present study, the parameters of nozzle for simulation are the droplet size and the spray velocity. The droplet size influences to fire flume on fire suppression more than the spray velocity because of the effect of the terminal velocity. The optimal condition for fire suppression is that the droplet size and the spray velocity are $100{\mu}m$ and 20 m/s respectively.

• Bulletin of the Korean Chemical Society
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• v.24 no.3
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• pp.334-338
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• 2003

A simple synthetic method for haptens of organophosphorus (OP) pesticides with a spacer arm (aminocarboxylic acid) attached at the pesticide thiophosphate group was developed and was applied to the synthesis of haptens for the OP fungicide tolclofos-methyl. Using the haptens, a selective enzyme-linked immunosorbent assay (ELISA) for tolclofos-methyl was developed. One of the haptens was coupled to BSA to use as an immunogen. Rabbits were immunized with this conjugate to obtain polyclonal antibodies to tolclofos-methyl. The antisera were screened against another hapten coupled to ovalbumin (OVA). Using the serum with highest specificity, an antigen-coated ELISA was developed, which showed an $IC_{50}$ of 160 ng/mL with the detection limit of 20 ng/mL. The antibodies showed negligible cross-reactivity with other OP pesticides. An antibody-coated ELISA was also developed, which showed an $IC_{50}$ of 410 ng/mL with a detection limit of 130 ng/mL.

• ETRI Journal
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• v.5 no.3
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• pp.3-8
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• 1983

In the area of computer network planning, a location-allocation-size problem is involved. Since multi-facility location-allocation-size problems are very complex in formulating a mathematical model, it is a usual practise to adopt alternative approaches, which give no optimal results, instead of the optimal solution by mathematical approach. In this article, however, an attempt is made to formulate a mathematical model for the decision making problem of computer network design.

• Bulletin of the Korean Chemical Society
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• v.24 no.1
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• pp.70-74
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• 2003

The heterogeneous bioluminescence immunoassay for digoxin was developed using photoprotein, native aequorin as a label and the site-directed immobilization technique based on avidin/biotin interaction. Aequorin is a bioluminescence protein, originally isolated from the jellyfish Aequoria Victoria and an attractive label in analytical applications because of sensitive detection due to virtually no background bioluminescent signal. Digoxin is a cardioactive drug, and its therapeutic level in serum is at low concentration with very narrow therapeutic index. The aequorin-digoxigenin conjugates were synthesized by the N-hydroxysuccinimide ester method and characterized in terms of bioluminescent residual activity. The resulting dose-response curve shows that the detection limit is $1.0\;{\times}\;10^{-10}\;M$ and a dynamic range is three orders of magnitude, which was obtained by $1.0\;{times}\;10^{-10}\;M$ conjugate and 0.9 μg/mL anti-digoxin antibody. Three structurally similar molecules to digoxin were examined for their cross-reactivity. None of these three compounds showed any crossreactivity with digoxin antibody employed in this study. Standard amounts of digoxin corresponding to the therapeutic range were spiked into the each serum solution. Study of the serum matrix effect indicated that correlation coefficient shows good agreement between luminescence light intensity between in buffer and in serum.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.05a
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• pp.715-717
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• 2016

Conventional drug carriers such as liposomes, nanoparticles, polymer micelles, polymeric conjugate and lipid microemulsion for cancer chemotherapy shield normal tissues from toxic drugs to treat cancer cells in tumors. However, inaccurate tumor targeting uncontrolled drug release from the carriers and unwanted accumulation in healthy sites can limit treatment efficacy with current conventional drug carriers with insufficient concentrations of drugs in the tumors and unexpected side effects as a result. In this research, we examined the use of sickle red blood cells as a new drug carrier with novel tumor targeting and controlled release properties. Sickle red blood cells show natural tumor preferential accumulation without any manipulation and controlled drug release is possible using a hemolysis method with photosensitizers.

• ETRI Journal
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• v.33 no.4
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• pp.517-527
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• 2011

Currently, there is a considerable interest in 3D object reconstruction using terrestrial laser scanner (TLS) systems due to their ability to automatically generate a considerable amount of points in a very short time. To fully map an object, multiple scans are captured. The different scans need to be registered with the help of the point cloud in the overlap regions. To guarantee reliable registration, the scans should have large overlap ratio with good geometry for the estimation of the transformation parameters among these scans. The objective of this paper is to propose a registration method that relaxes/eliminates the overlap requirement through the utilization of photogrammetrically reconstructed features. More specifically, a point-based procedure, which utilizes non-conjugate points along corresponding linear features from photogrammetric and TLS data, will be used for the registration. The non-correspondence of the selected points along the linear features is compensated for by artificially modifying their weight matrices. The paper presents experimental results from simulated and real datasets to illustrate the feasibility of the proposed procedure.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.33 no.1
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• pp.17-27
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• 2009

Water jet impingement cooling is used to remove heat from high-temperature surfaces such as hot steel plates in the steel manufacturing process (thermo-mechanical cooling process; TMCP). In those processes, uniform cooling is the most critical factor to ensure high strength steel and good quality. In this study, experiments are performed to measure the heat transfer coefficient together with the inverse heat conduction problem (IHCP) analysis for a plate cooled by planar water jet. In the inverse heat transfer analysis, spatial and temporal variations of heat transfer coefficient, with no information regarding its functional form, are determined by employing the conjugate gradient method with an adjoint problem. To estimate the two dimensional distribution of heat transfer coefficient and heat flux for planar waterjet cooling, eight thermo-couple are installed inside the plate. The results show that heat transfer coefficient is approximately uniform in the span-wise direction in the early stage of cooling. In the later stage where the forced-convection effect is important, the heat transfer coefficient becomes larger in the edge region. The surface temperature vs. heat flux characteristics are also investigated for the entire boiling regimes. In addition, the heat transfer rate for the two different plate geometries are compared at the same Reynolds number.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.38 no.11
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• pp.861-869
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• 1989

The optimization problems, based on Pontryagin's Maximum Principle, for minimizing (damping) Xenon spatial oscillations in Load Following operations of Pressurized Water Reactor (PWR) is presented. The optimization model is formulated as an optimal tracking problem with quadratic objective functional. The oen-group diffusion equations and Xe-I dynamic equations are defined as equality constraints. By applying the maximum principle, the original problem is decomposed into a single time problem with no constraints. The resultant subproblems are optimized by using the conjugate Gradient Method. The computational results show that the Xenon spatial oscillation is minimized, and the reactor follows the load demand of the electrical power systems while maintaining the desired power distribution.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.6 no.3
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• pp.216-225
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• 1994

By use of laboratory experiment data set for an elliptic shoal by Berkhoff et al. (1982), both accuracy and Performance tests of numerical results between PCGM (Preconditioned Conjugate Gradient Method) and PA(Parabolic Approximation) are compared. Although both results show good agreement with the experimental data the PA model gives better reproduction of the relatively high amplitudes in the section 4-5 downwave of the shoal, in comparison with the PCGM. The PA model has been proved to be a useful tool for predicting wave transformationsin large shallow water region, but it can be applied only to the case of negligible reflection. On the other hand, there is a need to improve the computational efficiency of the PCGM model which is a finite difference scheme directly derived from the mild slope equation and can handle reflection. By taking the results of th PA model as an input data of the PCGM, the CPU time can be reduced by about 40%.