• Journal of fish pathology
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• v.3 no.1
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• pp.11-19
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• 1990

Yellow tail (Seriola quinqueradiata) infected by Pasteurella piscicida have been occurred to mass mortality without showing apparent surface lesions in cage culture farms. In this case, it is necessary to consider countermeasure by rapid diagnosis of infected fish. The purpose of the present study was to investigate usefulness of the direct fluorescent antibody technique(FAT) for rapid diagnosis of pseudotuberclosis of cultured yellowtail caused by P. piscicida. Antibody produced by inoculating rabbit with formalin killed pseudotuberclosis bacteria antigen(strain KNP-2). Immunoglobulin-G(IgG) was purified from antisera by DEAE-cellulose column chromatography and conjugate with fluorescein isothiocyanate. Fluorescein-labeled antisera was purified by sephadex G-25 gel column chromatography. The fluorescein/protein molar ratio of labeled antisera was determined as 8.8-9.5. Diagnosis of cultured yellowtail was examined in cage culture farms which located in Tongyung, kyungnam from July to October 1990. The causative bacteria of pseudotuberclosis could be detected within two hours after the specimens were transferred to the laboratory for FAT, and it showed that FAT could be adapted as a rapid and accurate diagnostic method of pseudotuberclosis in yellowtail.

• Journal of Korean Society for Geospatial Information Science
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• v.17 no.4
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• pp.23-29
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• 2009

Recent growth of the geospatial information on the Web has made it possible to easily access a wide variety of geospatial information. An integration of different geospatial objects consists of the following three steps; extracting geospatial objects from the maps, converting the coordinate system and discovering pairs of objects that represent the same real-world entity in the two maps. This paper deals mainly with the third step to correspond conjugate objects and four matching types and criteria is presented. The techniques designed and developed can be utilized to efficiently integrate distributed heterogeneous spatial databases such as the digital maps and POIs from other data sources. To achieve the goal, we presented four types and criteria for the matching schema. The main contributions of this paper are as follows. A complete process of integrating data from maps on the Web is presented. Then, we show how attributes of the objects can be used in the integration process.

• Journal of Korean Society for Geospatial Information Science
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• v.22 no.3
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• pp.121-128
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• 2014

This study investigated the accuracy of DEM generated by heterogeneous stereo satellite images based on RPC. Heterogeneous sensor images with different spatial resolution are SPOT-5 panchromatic and IKONOS images. For the accuracy evaluation of the DEM, we compared the DEMs generated from two kinds of sensors and that produced using homogeneous SPOT-5 and IKONOS stereo images. As results of the evaluation, accuracy of 3D positioning by heterogeneous images was substantially similar to that of homogeneous stereo images for exact conjugate points. But, in terms of quality of the DEM, DEM generated by heterogeneous sensor showed a lower accuracy about twice in RMSE and about 3 times in LE90 than that of homogeneous sensors. As a result, DEM can be generated by using heterogenous satellite imagery. But if we use a stereo image with different spatial resolution, the performance of image matching was very important factor for the production of high-quality DEM.

• Journal of Food Hygiene and Safety
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• v.6 no.3
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• pp.111-117
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• 1991

We examined to develop the enzyme linked immunosorbent assay (ELISA) for determination of zearalenone in animal feeds. Zearalenone was first converted to 6'-(carboxymethyl) zearalenone oxime(zearalenone oxime) to get a coupling site and then conjugated to bovine serum albumin(BSA) for use as immunogen and to horseradish peroxidase(HRP) for use as enzyme marker. Antibody against zearalenone was obtained after 11 weeks of immunization of rabbit with zearalenone oxime-BSA. Cross reactivity of the antibody with ${\alpha}-zearalanol,\;{\beta}-zearalenol,\;{\alpha}-zearalanol\;and\;{\beta}-zearalanol$ were 168, 46, 26 and 20% respectiviely. A simple procedure was devised for the screening of zearalenone in feeds using ELISA. Feeds samples(5g) were extracted by blending with 25 ml of methanol-phospate butTered saline-dimethylformate(70 : 29 : 1) and the extract was filtered and aqueous filterate analyzed. It took only 1 hours to do whole procedure for the analysis of zearalenone in feeds by the direct competitive ELISA, and detectable limit was 1-100 ppb. Using this procedure, only 4 of 24 feed samples showed positive results with 3.93-7.43 ppb levels.

• KSBB Journal
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• v.22 no.4
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• pp.260-264
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• 2007

Peptides are frequently studied as candidates for new drug development. Recently, synthesized peptide library is screened for a certain functionality on a microarray biochip format. In this study, in order to replace the conventional cellulose membrane with glass for a microarray chip substrate for peptide library screening, we modified the glass surface from amines to thiols and covalently immobilized the peptides. Using trypsin-FITC (fluorescein isothiocyanate) conjugate that could specifically bind to a trypsin binding domain consisting of a 7-amino acid peptide, we checked the degree of surface modification. Because of the relatively lower hydrophilicity and reduced surface roughness, the conjugation reaction to the glass required a longer reaction time and a higher temperature. It took approximately 12 hr for the reaction to be completed. From the fluorescence signal intensity, we could differentiate between the target and the control peptides. This difference was confirmed by a separate experiment using QCM. Furthermore, a smaller volume and higher concentration of a spot showed a higher fluorescence intensity. These data would provide the basic conditions for the development of microarray peptide biochips.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.8C
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• pp.827-834
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• 2003

In this paper, we implemented an optical information security system using computer generated hologram based on the principle of interference and BaTiO$_3$that is photorefractive material. First of all, we would generate binary phase hologram which can reconstruct the original image perfectly, and regard this hologram as the image to be encrypted. And then applying the interference rule to the hologram, encrypted and reference (fkey information) images are generated. In the decrypting process, we can get an interference intensity by interfering the reference image and the encrypted image in the Mach-Zehnder interferometer. and transforming interference intensity information into phase information using LCD(liquid crystal display) and finally recover original image by inverse Fourier transforming the phase information. In this process, the Intensity information generated by interference of two images is very sensitive to external vibrations. So, we get a stable interference using the characteristic of SPPCM(self pumped phase conjugate mirror) of BaTiO$_3$that is photorefractive material. The proposed method has an advantage of double image encryption by encrypting the hologram of the image instead of original image.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.4
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• pp.523-533
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• 2000

In this paper, we present the hardware implementation of a smart antenna test-bed for a real -time performance analysis of the beam-forming algorithm operating in a wide-band CDMA environments of the WLL(Wireless Local Loop) standard. The test-bed introduced in this paper includes an external PC and signal generating module as well as the beam-forming module in order to perform, analyze, and evaluate the performance of the proposed smart antenna system. In the beam-forming module, the optimal weight vector is provided by the modified CGM algorithm. The computed weight vector is transferred back to the external PC for the performance analysis based on the off-line processing. From our analysis obtained in the hardware of the test-bed, it is concluded that the proposed smart antenna system for the WLL system is appropriate for enhancing the communication quality and capacity tremendously at the cell-site of the CDMA environment.

• Korean Journal of Optics and Photonics
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• v.13 no.6
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• pp.548-553
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• 2002

We have investigated the output characteristics of a Nd:YAG oscillator with a Stimulated Brillouin Scattering phase conjugation mirror (SBS-PCM). FC-75 is used as an SBS gain medium and Cr:YAG (T=50%) as a Q-switcher. The SBS-PCM consists of an SBS-cell and a simple plano-convex focusing lens system. For the case of not using a Q-switcher, an SBS-cell doesn't work as a phase conjugation mirror. For the case of using a Q-switcher, the energy characteristics remains nearly the same whether using the SBS-cell or not using it. The pulse width is reduced from 50 ns to 40 ns by an SBS-PCM. When the SBS-cell is used with a Q- switcher, the SBS-cell works very well as a phase conjugation mirror and its beam-profile is improved dramatically. And in order to explain the progression of beam-profile, we propose a simple model based on the nonlinear reflectivity of the SBS-PCM, whose reflectivity is dependent on the pumping intensity at the focal plane.

• Archives of Pharmacal Research
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• v.18 no.5
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• pp.336-339
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• 1995

Ephedra distachya cultures have been known to accumulate two major $\rho-coumaroylamino$ acids (p-coumaroylgicine and $\rho-coumaroylamino$ -D-alanine) by D-Ala treament. When D-Ala was added together with serial concentrations of yeast-derived elicitor, the accumulation of $\rho-coumaroylamino$ -D-Ala $(\rho-CDA)$ was geatly increased in an additive manner. In feeding experiments, $[1-^14C]$-D-Ala was incorporated into $\rho-CDA$at a rate of 2.2% or 2.3% of added radioactivity, indicating that exogenous D-Ala served as a precursor of the conjugate. $[1-^14C]$-L-Ala wasalso incorporated into p-CDA (0.23%) in the elicitor treated cultures. This fact suggested that at least a part of $(\rho-CDA)$ was produced from active conversion of L-Ala by the elecitation. In order to investigate a possible role of D-Ala as an elicitor of $\rpo-coumaroylamino$ acids $(\rpo-CAA)$, cold D-Ala was added together with labeled L-Ala. Although L-Ala seemed to be incorporated into $\rho-CDA$ by this treatment, the incorporation ratio was too small (0.054%) to draw a clear conclusion. However, the amount of $\rpo-coumaroylglycine$, which did not use D-Ala as a substrate, was also sightly increased by D-Ala treatment irrespective of the presence of elicitor, suggesting that exogenous D-Ala might act as an elicitor of$\rpo-CAA$ as well as a precusor substrate of $\rho-CDA$.

• Bulletin of the Korean Chemical Society
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• v.28 no.8
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• pp.1353-1357
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• 2007

Second-order rate constants (kN) have been determined spectrophotometrically for reactions of Y-substituted phenyl 2-furoates (1a-h) with piperidine and morpholine in 80 mol % H2O/20 mol % DMSO at 25.0 ± 0.1 oC. The Brønsted-type plot exhibits a downward curvature for the reactions with strongly basic piperidine but is linear for the reactions with weakly basic morpholine. The slope of the curved Brønsted-type plot changes from -1.25 to ?0.28 as the pKa of the conjugate acid of the leaving aryloxides decreases. The pKa at the center of the Brønsted curvature, defined as pKa°, was determined to be 6.4. The aminolysis of 1a-h has been concluded to proceed through a stepwise mechanism on the basis of the curved Brønsted-type plot. The reactions of Ysubstituted phenyl cinnamates (2a-g) with piperidine resulted in a curved Brønsted-type plot with a pKa° values of 6.4. However, the curved Brønsted-type plot has been suggested to be not due to a change in the RDS but due to a normal Hammond effect of a concerted mechanism, since the Brønsted-type plot for the corresponding reactions with morpholine results in also a curved Brønsted-type plot with a pKa° values of 6.1. The furoates with a basic leaving group (i.e., 1b-g) are less reactive than the corresponding cinnamates (i.e., 2b-g). The k2/ k-1 ratios for the reactions of 1b-h are much smaller than unity, which has been suggested to be responsible for their low reactivity.